Thermo Scientific Nicolet iS5 LS User Manual page 60

4
Troubleshooting the Instrument
56 Nicolet iS5 LS User Guide
2. Click the arrow button to continue.
The workflow collects a single-beam spectrum. When data collection is completed, the
single-beam spectrum is displayed along with an image of a reference single-beam
spectrum from a typical background measurement (using clean sample windows) along
with information about the expected energy ratio and the measured intensities at the
labeled hydrocarbon and water vapor peak locations.
3. Click the arrow button to continue.
The workflow collects another single-beam spectrum and ratios it against the one
collected previously to produce a 100% transmission spectrum (basically a straight line)
to show the level of random noise in the data. When data collection is completed, the
spectrum is displayed along with information about the expected peak-to-peak noise
-1
value at 1000 cm and the expected slope of the 100% transmission line.
4. Click the arrow button to continue.
The workflow asks if you want to measure wavelength accuracy and sample pathlength.
5. Select Run hexadecane and click continue.
6. Add two drops of 99% hexadecane to the sample cup, wait 2 seconds for the sample to
settle, gently close the sampling arm and then click continue.
Do not touch the sampling arm until the measurement is completed.
When data collection is completed, the spectrum is displayed along with information
about the expected peak locations and maximum peak deviation and the expected
pathlength range.
7. Click continue and thoroughly clean both sample windows.
8. Click continue to end the workflow.
Note To retrieve data from a Spectra Check workflow, use Data Hub.
Thermo Scientific