Thermo Scientific Nicolet iS5 LS User Manual page 32

3
Maintaining the Instrument
Calibrating the Sample Stage
28 Nicolet iS5 LS User Guide
Stage calibration verifies the distance between the two sample windows during measurements.
The distance is controlled by the motorized sample stage (lower window), which moves
vertically before and after each measurement. After you power up the instrument, allow
approximately 6 hours for the instrument to reach thermal equilibrium before you calibrate
the sample stage. Repeat stage calibration every three months.
Calibrate the sample stage immediately:
• After you have calibrated the laser frequency
• If the instrument failed the Pathlength portion of the
Before you calibrate the sample stage, make sure the sample windows are clean and dry. The
sampling arm should be closed.
There are two stage calibration tools:
• Quick Calibration (verifies or finely adjusts stage position)
• Full Calibration (recalibrates the stage)
NOTICE
• Run Full Calibration only if Quick Calibration fails or during preventive
maintenance for the instrument.
• Always calibrate the laser frequency
run Full Calibration.
To continue from one screen to the next in the workflow, click the
 To calibrate the sample stage
1. From the qPro main window, click System Tools and then click Quick Calibration (or
Full Calibration).
The workflow shows its historical results.
2. Make sure the sampling arm is closed and then click continue.
3. Make sure Start a new quick (or full) calibration is selected and then click continue.
4. Follow the instructions in the workflow. When the workflow is completed, a pass or fail
result is displayed. If stage calibration fails, consult the
Troubleshooting section.
5. Click continue to return to System Tools.
6. Click the Home button at the top of the qPro window to return to the home screen.
Calibration Check
and then align the instrument optics
Stage calibration failed
before you
button.
topic in the
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