Thermo Scientific Nicolet iS5 LS User Manual page 31

Table of Contents

Calibrating the Laser Frequency

Thermo Scientific

Laser calibration verifies the known frequency of the instrument laser. The laser frequency

may drift slightly over time, particularly in locations that have large temperature swings or

excessive vibration. After you power up the instrument, allow approximately 6 hours for the

instrument to reach thermal equilibrium before you calibrate the laser frequency. Repeat laser

calibration every three months.

NOTICE Always

align the instrument optics

calibrate the laser frequency.

Calibrate the laser frequency immediately:

• after you have changed the source

• if the instrument failed the Wavelength Accuracy portion of the

• Before you align the instrument or calibrate the sample stage

Before you calibrate the laser frequency, make sure the sample windows are clean and dry.

The sampling arm should be down.

To continue from one screen to the next in the workflow, click the



To calibrate the laser frequency

1. From the qPro main window, click System Tools and then click Laser Calibration.

The workflow shows its historical results.

2. Make sure the sampling arm is closed and click continue.

3. Make sure Start a new laser calibration is selected and then click continue.

Follow the instructions in the workflow. When the workflow is completed, a pass or fail

result is displayed. If laser calibration fails, consult the

topic in the Troubleshooting section.

4. Click continue to return to System Tools.

5. Click the Home button at the top of the qPro window to return to the home screen.

After laser calibration is completed,

Troubleshooting