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Teledyne ACCQPrep HP150 Operation Manual
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Teledyne ACCQPrep HP150 Operation Manual

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ACCQPrep HP150
Operation Guide
69-5233-899
Rev B, October 2024
© 2019 Teledyne Instruments, Inc. All rights reserved.
Use and Disclosure of Data: Information contained herein is classified as EAR99 under the U.S. Export
Administration Regulations. Export, reexport or diversion contrary to U.S. law is prohibited.

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  • Page 1 69-5233-899 Rev B, October 2024 © 2019 Teledyne Instruments, Inc. All rights reserved. Use and Disclosure of Data: Information contained herein is classified as EAR99 under the U.S. Export Administration Regulations. Export, reexport or diversion contrary to U.S. law is prohibited.
  • Page 3 OSHA Guide 1910.1000. To reduce those levels to a safe exposure, Teledyne ISCO recommends that you place the system in a laboratory hood designed for the purpose of ventilation. This hood should be constructed and operated in accordance with federal state and local regulations.
  • Page 4 ACCQPrep HP150 Installation and Operation Guide Safety Warnings DANGER DANGER – limited to the most extreme situations to identify an imminent hazard, which if not avoided, will result in death or serious injury. Hazard Symbols The equipment and this manual use symbols used to warn of hazards.
  • Page 5 ACCQPrep HP150 Installation and Operation Guide Safety Warnings For Additional Technical assistance for the Teledyne ISCO ACCQPrep HP150 Information can be obtained from: Teledyne ISCO 4700 Superior St. Lincoln NE 68504 Phone: (800) 775-2965 or (402) 464-0231 Fax: (402) 465-3001 E-mail: IscoService@teledyne.com...
  • Page 6 ACCQPrep HP150 Installation and Operation Guide Safety Warnings EAR99 Technology Subject to Restrictions Contained on the Cover Page...
  • Page 7 ACCQPrep HP150 Table of Contents Section 1 Introduction 1.1 Overview ............1-1 1.2 Product Overview .
  • Page 8 5.5 ACCQPrep HP150 Maintenance ........
  • Page 9 ACCQPrep HP150 Table of Contents 5.8.11 Capillary Inlet Cone Installation ....... 5-26 5.8.12 PurIon Troubleshooting .
  • Page 10 ACCQPrep HP150 Table of Contents EAR99 Technology Subject to Restrictions Contained on the Cover Page...
  • Page 11 The ACCQPrep HP150 is available in different configurations to meet your needs. An optional Solvent Selector Valve Module sup- ports up to three choices for solvent A and three different choices for solvent B.
  • Page 12 ACCQPrep HP150 with optional ELSD – Th is sy stem h as the same high performance features as the ACCQPrep HP150 system, but includes an internal evaporative light scattering detector (ELSD).
  • Page 13 The system also supports TCP/IP communication. This allows direct control of the system by an external computer between Ethernet ports of the ACCQPrep HP150 system and the com- puter. TCP/IP communication also allows remote control of the system via an established network.
  • Page 14 ACCQPrep HP150 Section 1 Introduction Figure 1-2 Location of power cord for the ACCQPrep 1.5 Specification Table 1-1 ACCQPrep HP150 Dimensions of ACCQPrep 27.5 x 14.0 x 20.0 in (69.9 x 35.6 x 50.8 cm) (Footprint without column installed) Dimensions of ACCQPrep AS 2x1 18.9 x 14.0 x 19.3 in (48.0 x 35.6 x 49.0 cm)
  • Page 15 Maximum Altitude 2000 meters Note 1. All specifications are subject to change. Table 1-2 ACCQPrep HP150 with PurIon System Dimensions (H x W x D) ACCQPrep: 27.5 x 14.0 x 20.0 in (69.9 x 35.6 x 50.8 cm) Mass Spectrometer 26 x 11 x 22 in (66 x 28 x 56 cm)
  • Page 16 ACCQPrep HP150 Section 1 Introduction Table 1-3 Component Materials List Pressure Transducer 316 and 17-4 PH stainless steels and perfluorelastomer (FFKM) AutoSampler Wash Pharm-A-Linepolypropylene EAR99 Technology Subject to Restrictions Contained on the Cover Page...
  • Page 17 ACCQPrep HP150 Section 2 Configuration 2.1 Configuration of the The C window (T > C ) groups ONFIGURATION OOLS ONFIGURATION its settings under tabs placed along the top of it. ACCQPrep Solvent settings, the default tube volume, the system name, and the time zone setting (Figure 2-1) can be found under T >...
  • Page 18 You can also R OLVENT EMOVE names that you no longer use. OLVENT Modifier Type (ACCQPrep HP150 with 3rd Solvent Modifier Module) – This allows you to choose which solvent your modifier is dissolved in. Solvent Level Sensing – • E...
  • Page 19 IT department. For more infor- mation about networking, see TN28 Networking Guidelines for CombiFlash Products available at www.teledynelabs.com. See 4.3.12 Network Configuration options for more information about using PeakTrak to network the ACCQPrep HP150. 2.3 User Preferences > C > U...
  • Page 20 ACCQPrep HP150 Section 2 Configuration The pressure trace doesn’t have a vertical scale with pressure. To view the actual pressure at a point in time, refer to the status bar in the upper right corner of the screen during a separation. If you are reviewing a previous separation, this information isn’t available on...
  • Page 21 ACCQPrep HP150 Section 2 Configuration name of the column used for the separation is recorded on the report. This name can be descriptive for the column, or identify it exactly using the column serial number. Probe Type – This defaults to the standard PTFE probe sup- plied with the instrument.
  • Page 22 ACCQPrep HP150 Section 2 Configuration To delete a column at C > P HPLC: 2.4.2 Delete a Column ONFIGURATION 1. Ensure the column to be deleted is not selected in the col- umn position field(s). 2. Select the column you would like to delete from the C list.
  • Page 23 ACCQPrep HP150 Section 2 Configuration 5. The newly created method uses the default method parameters. To modify the new method: a. Select E . The M opens. ETHOD DITOR b. Make changes as desired in the M ETHOD DITOR c. E when completed.
  • Page 24 ACCQPrep HP150 Section 2 Configuration Figure 2-6 Scouting Method definition window Once a scouting gradient has been defined, load the column and the scouting method: 1. In the Main Screen, select the column that you want to use for the run.
  • Page 25 ACCQPrep HP150 Section 2 Configuration Figure 2-8 Scouting Run screen screen will appear upon completion of the run: Figure 2-9 Run screen at end of run Next, define the focused gradient: 1. Select F from the bottom right of the Run...
  • Page 26 See Section 3.4 Operation Troubleshooting (Peaks elute too early and Peaks elute too late) and the Teledyne’s Technical Note Focused Gradients— What Do You Mean the Compound Eluted Too Early or Too Late?! (TN61).
  • Page 27 ACCQPrep HP150 Section 2 Configuration Scheduled Operations are useful to • wash all installed columns at the end of the day to remove buffers and modifiers, potentially improving column life, • wash strongly retained samples from columns, and • periodically run reference standards to test retention times and peak shapes.
  • Page 28 ACCQPrep HP150 Section 2 Configuration Before a scheduled operation is run, make sure that the required solvents and samples are in place. Samples need to be placed in the sample rack at the location listed in the saved sample queue.
  • Page 29 ACCQPrep HP150 Section 3 Operation 3.1 Introduction The ACCQPrep HP150 is unique in the Preparative HPLC market. There are several methods of operation possible that can be selected to fit your needs. Methods contain all of the information used during a separation of a single sample, such as gradient profile, detection and peak cutting parameters, and fraction size.
  • Page 30 ACCQPrep HP150 Section 3 Operation 3.3 Starting a Separation To separate a single sample, select S to start a separation. TART The M window opens. This allows INIMUM EQUIREMENTS you to select an injection technique and select where the frac- tions will be collected (the starting rack and tube position).
  • Page 31 ACCQPrep HP150 Section 3 Operation 2. Select S to start a separation as you would for an indi- TART vidual separation. The M win- INIMUM EQUIREMENTS dow opens; in front of that, the S window AMPLE PTIONS opens. 3. If you want to automatically perform multiple injections...
  • Page 32 ACCQPrep HP150 Section 3 Operation Figure 3-2 Selecting the sample volume and number of injections If the I exceeds 50% of the loop volume, a NJECTION OLUME warning message will appear recommending a change in the parameters. This message can be ignored at your discretion.
  • Page 33 ACCQPrep HP150 Section 3 Operation 7. Select F > O to view previous separations. If a sample had multiple injections, the run sequence will be dis- played under a single name preceded by a “+” symbol. This symbol indicates there are multiple injections with the same base file name.
  • Page 34 ACCQPrep HP150 Section 3 Operation 3. Near the end of the equilibration, the inject valve moves to the sample load position. 4. The AutoInjector Module aspirates enough sample to fill the loop with the programmed amount and to compensate for the volume of the sample probe.
  • Page 35 Once installed the following direction can be followed for using the Dilute Sample Load Pump. To Prime the Dilute Sample Load Pump: 1. Ensure that your ACCQPrep HP150 is primed. 2. Place the Dilute Sample Loading Pumps inlet line into a weak solvent.
  • Page 36 ACCQPrep HP150 Section 3 Operation Figure 3-4 Loading Pump P button The Dilute Sample Load Pump can be ran manually through the pump face interface or set up to run automatically (with user prompts) with programmed volume and flow rate. With the manual valve, the user does need to be present for user-prompted action to turn the valve as needed.
  • Page 37 ACCQPrep HP150 Section 3 Operation Figure 3-5 Dilute Sample Loading Pump Automatic Loading window Figure 3-6 The Large Sample Injection window By default, the flow rate is the same as that for the column. However, the HPLC pumps can also be run at a different...
  • Page 38 ACCQPrep HP150 Section 3 Operation • P – Type or select the pump’s volume in OLUME mL, or choose U NLIMITED · U – The pump runs until you stop it. The NLIMITED pumps can be restarted to allow for flask washings to be loaded.
  • Page 39 ACCQPrep HP150 Section 3 Operation To run a queue, do one of the following: • Select the R tab, then select S TART • Select the “≡” menu on the first unfinished run, then select S TART In either case, M opens.
  • Page 40 ACCQPrep HP150 Section 3 Operation columns at the end of the workday. This removes strongly retained compounds and washes buffers and solvent modifiers from the system. To reuse an existing run: On the Q tab, open the run you Other queue operations...
  • Page 41 ACCQPrep HP150 Section 3 Operation ≡ – A menu to edit a sample. Menu options only apply to the row from which they are chosen.. • S or R starts the separation. R TART starts a separation regardless of its order in the queue.
  • Page 42 ACCQPrep HP150 Section 3 Operation Sample Position – The location of the sample for this sepa- ration. If the sample size is too large for a single vial, create a second line in the queue and access the second vial.
  • Page 43 ACCQPrep HP150 Section 3 Operation injection minimizes the risk of sample crash by placing a small amount of a user selected solvent on each end of the sample fluid. All sample injection steps are listed below for reference. 1. During column equilibrium, the sample loop is first placed Default Injection Sequence into the run position.
  • Page 44 ACCQPrep HP150 Section 3 Operation 1. The inject valve is moved to bypass to prevent contamina- tion of the loop during the cleaning process. 2. Air is drawn into the probe to eliminate any remaining sol- vent in the probe.
  • Page 45 ACCQPrep HP150 Section 3 Operation volume of the sample probe. Since the loop is still in the separation position, the wash solvent is sent to waste. 10. The loop is switched to the load position. 11. The programmed injection volume is aspirated into the sample loop.
  • Page 46 ACCQPrep HP150 Section 3 Operation 4. AutoSampler right rack (D). If racks are placed in both the ACCQPrep and AutoSampler 4x2, available racks will be used in the following order: 1. ACCQPrep left rack (A), 2. ACCQPrep right rack (B), 3.
  • Page 47 ACCQPrep HP150 Section 3 Operation Note Verify that there are no impurities eluting after the desired compound(s). a. Start with a scouting gradient run either from analyti- cal HPLC data or from scouting data from an ACCQPrep HPLC system. b. Select F...
  • Page 48 ACCQPrep HP150 Section 3 Operation h. Select S on the R window. TACK NJECTIONS IEWER The S window opens. The system dis- TACKED NJECTION plays a simulated stacked injection based on the isocra- tic run. The time windows displayed are based on the isocratic peak collection.
  • Page 49 ACCQPrep HP150 Section 3 Operation Figure 3-10 The Stacked Injections Window Show Single Injection – When selected, the chromatogram displays a single injection. This view is useful for adjusting the time windows. For example, adjusting the time window to appear a bit larger than the collected peak can maximize sample recovery by collecting the peak’s tail.
  • Page 50 ACCQPrep HP150 Section 3 Operation Collection – Determines which peaks are collected, if any. Col- lection interacts with Fraction Pooling to determine peak col- lection behavior, as summarized in Table 3-1. Table 3-1 Time window collection Fraction Pooling on Fraction Pooling off...
  • Page 51 ACCQPrep HP150 Section 3 Operation · Inadequate priming of the pump may cause the A or B solvent to flow at lower rates. This may be due to sticking check valves. Open the front panel and tap the check valves with a wrench while priming to assist in bubble passage through the pump.
  • Page 52 ACCQPrep HP150 Section 3 Operation should be visible with a small gap between the rods. · This is not a lamp or detector hardware problem. These failures have corresponding error messages. Peaks elute too early – • Solvent A not being delivered properly. Check solvent...
  • Page 53 ACCQPrep HP150 Section 3 Operation • Impurities in solvent; evaluate with runs with no sample injection to determine if impurity is from the sample. Make a run with a standard injection volume, then double the equilibration time. If the peak in question become larger, it is solvent related.
  • Page 54 ACCQPrep HP150 Section 3 Operation · Semivolatile compounds may be difficult to detect or may benefit from modification of the ELSD sensi- tivity, gain or operating temperatures. Refer to the discussion on ELSD operation in section 4.3.4 before adjusting the ELSD operating temperatures.
  • Page 55 ACCQPrep HP150 Section 3 Operation e. Remove guard column (if installed) and check back pressure. If this corrects the problem, replace the guard column or following the wash- ing procedure below. f. Remove preparative column and check back pressure. g. If the column is clogged, try flushing with stron- ger solvent than mobile phase: (this procedure may damage polymeric reverse phases).
  • Page 56 ACCQPrep HP150 Section 3 Operation • Air leak in inlet lines: · Remove front panel and examine inlet lines while operating. If small air bubbles exist, follow tubing back to source, examining for source of air bubbles. Injection valve dripping –...
  • Page 57 ACCQPrep HP150 Section 4 PeakTrak 4.1 Overview PeakTrak is the software that controls the ACCQPrep. This section discusses the basic functionality of PeakTrak when operated via the touchscreen or a remote browser window. The M screen displays the current M .
  • Page 58 ACCQPrep HP150 Section 4 PeakTrak Figure 4-1 The Main screen Note The available commands and options will change according to the active window, the state of the system, and the current file. PeakTrak controls the separations performed by the system 4.1.2 Method Files...
  • Page 59 Method files use an *.pmtd filename extension. Default Methods When PeakTrak is started, the system loads a D EFAULT ETHOD contains Teledyne ISCO’s recommended EFAULT ETHOD basic and advanced settings for the use of a default column (as selected in the C screen).
  • Page 60 ACCQPrep HP150 Section 4 PeakTrak 4.2.1 File Figure 4-2 The File Menu The File menu lists the following commands: New – Select the N command to open a new method file. PeakTrak will open the M window using the default program settings for the default configured column size.
  • Page 61 ACCQPrep HP150 Section 4 PeakTrak Note are an exception and cannot be overwritten EFAULT ETHODS by the S command. If a D is open ETHOD EFAULT ETHOD and you attempt to save any modifications to that method, the window appears so you can rename the method file. This ILES preserves the default method.
  • Page 62 ACCQPrep HP150 Section 4 PeakTrak computer, PeakTrak displays a window from which you can save the M ETHOD Save Queue As – Saves the queue on the system’s internal hard drive for later use. The file name will have the .queue extension.
  • Page 63 ACCQPrep HP150 Section 4 PeakTrak Ionization settings – This command opens the I ONIZATION window to allow creation of custom ionization ETTINGS parameters that can be saved for various compound classes and projects. This command is not available from a remotely connected computer via a web browser.
  • Page 64 ACCQPrep HP150 Section 4 PeakTrak The Tools menu provides options to configure and manually control your ACCQPrep system: Manual Control – Choose this command to open the window from which you can operate the ANUAL ONTROL system manually. Manual control can assist with priming or purging the internal plumbing and aid system troubleshooting.
  • Page 65 If you are accessing the system over a network, the file will be saved to your PC; otherwise, they will be saved to a connected USB drive. Teledyne ISCO service personnel can interpret this data to optimize system performance or to troubleshoot difficulties.
  • Page 66 ACCQPrep HP150 Section 4 PeakTrak · Icon Finder (Section 4.3.6) · MS Manual Control (Section 4.3.7) · MS Manual Ionization Settings (Section 4.3.8) • T menu windows OOLS · Manual Control (Section 4.3.9) · Automation Manual Control (Section 4.3.10) · Large Sample Manual Control (Section 4.3.11) ·...
  • Page 67 ACCQPrep HP150 Section 4 PeakTrak Figure 4-7 The Run Screen at the end of a run Gradient Plot – The gradient plot area depicts the current gradient that will be used for the run. Depending on the setting to the CV...
  • Page 68 ACCQPrep HP150 Section 4 PeakTrak When the spectrum is displayed, touching the chromatogram at any point prior to the current elution time displays a spectrum at that point in time; the elution time corresponding to the spectrum is displayed under the spectrum.
  • Page 69 ACCQPrep HP150 Section 4 PeakTrak Solvent Selector Valve Module is installed, this control allows you to select a solvent for use in the current separation. Run Control Buttons – From left to right, the control buttons are: • S (the “play” symbol) — Starts or resumes the run.
  • Page 70 ACCQPrep HP150 Section 4 PeakTrak using the new run length. This modified method is ready for the next run, or can be saved for future runs Peak Collection Buttons – Three peak collection buttons are located at the bottom of the M window.
  • Page 71 ACCQPrep HP150 Section 4 PeakTrak • File and Folder operation buttons: · C — Copies a highlighted file to the system’s clipboard. · P — Pastes a file from the clipboard. If the file ASTE already exists in the current folder, the system will ignore the Paste command to prevent the original file from being overwritten.
  • Page 72 ACCQPrep HP150 Section 4 PeakTrak File > Set Data Path is primarily used with the U ANAGEMENT feature. U will automatically create a folder for ANAGEMENT each user. When using the system, set the data path to your folder or a subfolder within. Each user has a different data path.
  • Page 73 ACCQPrep HP150 Section 4 PeakTrak Figure 4-9 Method Editor screen with Edit Gradient panel Button bar – Allows the opening, editing, and saving of methods. Saved methods can be retrieved for further editing or use. Figure 4-10 Method Editor Button Bar...
  • Page 74 ACCQPrep HP150 Section 4 PeakTrak used” will help you determine if any solvent remaining in the column will be miscible with the solvent currently used in the system. To view the Column Data window, open the M window, then select C...
  • Page 75 ACCQPrep HP150 Section 4 PeakTrak For the I , type or select the volume of eluent NITIAL ASTE that is allowed to pass through the column to the waste port after the sample is injected. Use this feature to con- serve collection tubes by diverting all fluids during this time period, even if a peak is detected.
  • Page 76 ACCQPrep HP150 Section 4 PeakTrak Gradient Plot Area – T he gr adi en t plot a rea depic ts t he current gradient profile that will be used for the run. The X-axis of the plot displays the run time in minutes or in column volumes, depending on the setting to the CV CV toggle.
  • Page 77 ACCQPrep HP150 Section 4 PeakTrak depending on the setting to the CV CV toggle. (See Button bar.) IME TO Note This will change the scale of the X-axis on the gradient. Points that define the gradient will be automatically scaled to fit the new run length.
  • Page 78 ACCQPrep HP150 Section 4 PeakTrak Peak Collection – Sets the collection mode. Figure 4-14 Method Editor Peak Collection • A — Collects all fluids in the fraction collection tubes during a run. • P — Ccollects only eluted peaks in the fraction EAKS collection tubes during a run.
  • Page 79 ACCQPrep HP150 Section 4 PeakTrak Note You can select up to four peak detection options on the ACCQPrep systems,. If using more than one option, such as  with  , the system considers a peak to be present when an option is true (a logical OR).
  • Page 80 ACCQPrep HP150 Section 4 PeakTrak •  and  Wavelength Peak Detection (wavelength 1 and 2) — Enable these options to use and configure primary and secondary wavelength detection.  is set to monitor (displayed but not used for peak detection and cutting) by default.
  • Page 81 ACCQPrep HP150 Section 4 PeakTrak settings. These settings include the slope-based and peak width options described for  and  above. Here, is used to determine solvent absorbance IDTH suppression. Peaks that are twice the peak width are deemed to be solvent or other baseline drift and their absorbance spectrum will be subtracted as an assumed baseline spectrum.
  • Page 82 ACCQPrep HP150 Section 4 PeakTrak Mass Spectrometer Peak • Mass Spectrometer – Monitors or detect compounds Detection with a PurIon mass spectrometer system (PurIon systems only). Mass-directed peak detection can be set for up to 6 masses or 5 masses and 1 range.
  • Page 83 ACCQPrep HP150 Section 4 PeakTrak instructs peak collection to use the S noise TART threshold. Selecting T deselects M HRESHOLD ONITOR · Monitor – When checked, prevents fractionation based on the mass spectrometer signal. Selecting M deselects T ONITOR HRESHOLD ·...
  • Page 84 ACCQPrep HP150 Section 4 PeakTrak · MS Detection Start/End – Sets the start and end times at which the MRA valve is active during the method. This avoids salts and other contaminates at the start of a run and prevents unwanted compound from being sent to the mass spectrometer during a column wash.
  • Page 85 ACCQPrep HP150 Section 4 PeakTrak Figure 4-18 Gradient Table – Add a time point before the point whose row NSERT OINT is highlighted on the table. The new point will appear at the same location as the highlighted row’s point. You can move it along the X-axis by changing the or CV entry.
  • Page 86 ACCQPrep HP150 Section 4 PeakTrak MS > Method Development MS M enables a user to test and verify ion- ETHOD EVELOPMENT ization conditions for their compound. This command is not available from a remote connection. Figure 4-19 Mass Spectrometer Method Development...
  • Page 87 ACCQPrep HP150 Section 4 PeakTrak sample. To inject a sample, move the PurIon injection valve to the “inject Sample position” • Inject the sample (>20 uL) and move the valve to the “Scan Mass” position. Note Only use 22 gauge square tip needles (PN 29-9001-911) to avoid scratching the internal surfaces of the injection valve! Fil- ter the sample with a 0.45 µ...
  • Page 88 ACCQPrep HP150 Section 4 PeakTrak and report multimers, solvent adducts, and charge carriers that replace protons such as sodium and potassium. It can detect pos- itive, negative, and multiply charged ions. Once ions are detected, you can select any such ions that corre- spond to the mass of interest and use them for detection.
  • Page 89 ACCQPrep HP150 Section 4 PeakTrak Figure 4-20 The Ion Finder window • D – The molecular weight of your ESIRED compound, rounded to the nearest integer. • D – The list of possible adducts, ETECTION fragments, or multiply charged ion species detected.
  • Page 90 ACCQPrep HP150 Section 4 PeakTrak Figure 4-21 Mass Spectrometer Manual Control window • Start Carrier Pump- Turns the carrier pump on for priming or to replace one carrier solvent with another one. A purge is complete in ~5 minutes. This is replaced by S when the pump is running.
  • Page 91 ACCQPrep HP150 Section 4 PeakTrak Figure 4-22 Mass Spectrometer Ionization Settings window • Mass Spectrum Graph - Displays the mass spectrum. There are two mass spectra displayed. Blue displays the maximum of the current injection. Red displays the maximum of the previous injection for comparison.
  • Page 92 ACCQPrep HP150 Section 4 PeakTrak (PurIon and PurIon S) or 2000 Da (PurIon L). Changing the mass range defined by these controls may cause the Y-axis (ion count) scaling to change based on the tallest peak within the range. • Fluid Interface Pressure - The carrier solvent pressure is displayed in a ribbon gauge.
  • Page 93 ACCQPrep HP150 Section 4 PeakTrak • Span - Span voltage defines an increased voltage applied as the mass increases. As with the offset, larger values increase fragmentation. • Close - Closes the window. can assist with method development, mainte- 4.3.9 Manual Control...
  • Page 94 ACCQPrep HP150 Section 4 PeakTrak This opens the Automation Manual Control window, which can 4.3.10 Automation Manual be u se fu l in flus hi ng differen t por tion s of th e Aut oIn- Control jector/AutoSampler flow paths by choosing he flow path, fluid source, and the amount of fluid to be pumped by the AutoInjector.
  • Page 95 ACCQPrep HP150 Section 4 PeakTrak • Flow Rate – Type or select the pump’s flow rate in mL/min. • Pump Volume – Type or select the pump’s volume in mL, or choose U NLIMITED · U – The pump runs until you stop it; you...
  • Page 96 ACCQPrep HP150 Section 4 PeakTrak When this information is known, you can proceed with con- figuring system for network printing. Follow the on-screen instructions for entering the address, queue, and printer type information. After successfully printing a test page, the network printer will be available for printing using the F >...
  • Page 97 ACCQPrep HP150 Section 4 PeakTrak be reset when logging out. Use F > S select your folder. To prevent unauthorized changes to user management, this function is password protected. • To delete a user, select the D button next to the ELETE user’s name and confirm the action.
  • Page 98 ACCQPrep HP150 Section 4 PeakTrak Editing a method allows you to tailor the operation of the 4.4.1 Editing a method ACCQPrep system to best separate or purify the compounds of interest. PeakTrak has two types of method settings: basic and advanced.
  • Page 99 ACCQPrep HP150 Section 4 PeakTrak When using the A or P options, you can specify the EAKS maximum peak and non-peak volumes to be collected in the tubes. Be sure to enter volumes less than the maximum tube capacity. Different peak and non-peak tube volumes can be used...
  • Page 100 ACCQPrep HP150 Section 4 PeakTrak • All Wavelength Detection: Enable this option to detect peaks within a user-selected range of wavelengths. When enabled, select D to configure additional ETAILS settings. These settings include the slope-based and peak width options described in 1 and 2 above.
  • Page 101 ACCQPrep HP150 Section 4 PeakTrak touchscreen, this storage device is a USB Flash Drive con- nected to the USB port below the display. If connected to the system via an Internet browser, the storage device may be any device that can be accessed by the local computer.
  • Page 102 ACCQPrep HP150 Section 4 PeakTrak As a simple example, assume that a separation is developed with 2 compounds that elute at 5 and 10 minutes. At the 5 minute point of the separation, the sample loop can be removed from the flow path and loaded with another sample of the same materials.
  • Page 103 ACCQPrep HP150 Section 4 PeakTrak fractionation based on spectral purity. The spectral purity algo- rithm doesn't will fail on saturated peaks (flat on top due to detector saturation). To display the ratio, open the M window and select ETHOD DITOR the S option.
  • Page 104 ACCQPrep HP150 Section 4 PeakTrak device. If you are attempting to save a TXT file from the touch panel display, insert a USB Flash drive in the USB port below the display panel. • Use pinch zoom or pan to view the results screen in greater detail.
  • Page 105 ACCQPrep HP150 Section 4 PeakTrak Reading the Chromatogram – The plot area displays the following: · The red absorbance trace produced by the system’s peak detector. Absorbance units (also shown in red) that correspond to this trace are shown on the left Y-axis.
  • Page 106 ACCQPrep HP150 Section 4 PeakTrak Raw Lamp Energy The left side of the M window shows a R ANUAL ONTROL gauge. This provides an indication of the UV light mea- NERGY sured by the optical detection system at 254 nm. High lamp energy (green) means that the flow cell easily passes through a sufficient UV light source.
  • Page 107 Section 5 Maintenance 5.1 Introduction This section will cover some common maintenance routines for the ACCQPrep HP150. To clean the exterior surfaces, use a cleaning cloth dampened 5.1.1 Cleaning with a mixture of distilled water and a mild detergent. Use iso- propyl alcohol for tougher stains.
  • Page 108 ACCQPrep HP150 Section 5 Maintenance cord can be unplugged, or use a power strip where the plug can quickly be removed from the outlet in the event of an emergency. When you first place the system in S , internal compo-...
  • Page 109 ACCQPrep HP150 Section 5 Maintenance Vacuum or pressurized air applied to the outlet end of the drain tube must be present at the collection tray drain hole. Figure 5-1 Fraction collection tray drain hole 5.3 Preventive The system requires preventive maintenance for safe and reliable operation.
  • Page 110 ACCQPrep HP150 Section 5 Maintenance 5.4 Flow Cell Cleaning As a preventive measure, all default column methods finish the 5.4.1 Post Separation separation run with a high percentage of solvent B (Figure 5-2). This brief time (one to six column volumes) of strong solvent flushes residual compounds from the column, flow cell, and internal tubing.
  • Page 111 ACCQPrep HP150 Section 5 Maintenance When the lamp energy is lower than normal, the system will rec- 5.4.2 Quick Cleaning when ommend flow cell cleaning (Figure 5-3) before starting a sepa- Recommended ration run. You can then choose one of the following: •...
  • Page 112 ACCQPrep HP150 Section 5 Maintenance Figure 5-4 Manual Control Settings - Quick Cleaning 5. Select T HPLC C for the F option. HROUGH OLUMN 6. Select N for the Pump into Tube # option. By pumping into a collection tube, the diverter valve is also cleaned of any residue during this operation.
  • Page 113 EQUIRED and monitor the Raw Lamp Energy. (Figure 5-3) If the lamp energy is in the green range return the system to operation. If the lamp energy is red, contact Teledyne ISCO’s Technical Service department for assistance. 5.5 ACCQPrep HP150...
  • Page 114 ACCQPrep HP150 Section 5 Maintenance Gap will appear here Screws Figure 5-5 Location of the stator retaining screws 2. Remove the screws and pull the stator outward. This may require a side-to-side motion until removed. 3. When the stator is removed, use your finger or a small tool to pry the black plastic rotor out of its recess (Figure 5-6).
  • Page 115 ACCQPrep HP150 Section 5 Maintenance 5. Clean and inspect the stator sealing surface (which con- tacts the rotor) for scratches. If this part is scratched it may need replacement also. Installing the New 1. Place the new rotor into the recess with the Injection Valve Rotor passages facing outward.
  • Page 116 ACCQPrep HP150 Section 5 Maintenance Figure 5-9 Removing the fasteners 9. Carefully pull the pump head forward and off the guide pins. Pull straight and slowly to prevent damage to the pis- ton (Figure 5-10). Note The seal back-up washer may remain on the piston. Remove the washer from the piston if it did not stay in the pump head.
  • Page 117 ACCQPrep HP150 Section 5 Maintenance Figure 5-11 Removing the pump spacer block 11. Insert the flanged end of the seal insertion/removal tool into the seal cavity on the pump head. Tilt it slightly so that the flange is under the seal, and pull out the seal (Figure 5-12).
  • Page 118 ACCQPrep HP150 Section 5 Maintenance Figure 5-13 Cleaning the piston 13. Place a replacement seal on the rod-shaped end of the seal insertion/removal tool so that the spring (energizer) is visi- ble when the seal is fully seated on the tool. Insert the tool into the pump head (Figure 5-14).
  • Page 119 ACCQPrep HP150 Section 5 Maintenance Figure 5-15 Reinstalling the pump spacer block 15. Place the seal back-up washer on the piston. Replace the pump head. Make sure that the inlet check valves are on the bottom and the outlet check valves are on top (Figure 5-16).
  • Page 120 3000 psi. The following instructions are for the removal and replacement 5.5.4 Check Valve of the pump check valves for the ACCQPrep HP150 system. Replacement 1. Turn the power O 2. Access the pump compartment by grabbing the edges of front panel and pulling forward on both sides (Figure 5-18).
  • Page 121 ACCQPrep HP150 Section 5 Maintenance 3. The solvent supply lines to the pumps have fluid in them that will be drained during this procedure. If it is accept- able to drain this fluid back into the supply bottles, follow step ‘a’ below, if not, go to step ‘b’.
  • Page 122 ACCQPrep HP150 Section 5 Maintenance Inlet lines Outlet lines Pump drive connector Figure 5-20 Pump assembly 7. Pull the pump assembly forward to the position shown in Figure 5-21. Figure 5-21 Pump assembly 8. With the pump assembly pulled out, remove the inlet fit- tings connected to the bottom of the pump head.
  • Page 123 ACCQPrep HP150 Section 5 Maintenance 9. Using a Phillips screwdriver, remove the ‘Y’ fitting located above pump heads (Figure 5-23). Figure 5-23 Location of tee mounting screws 10.Using a " wrench, remove the check valve housings from the top and bottom of the pump heads (Figure 5-24).
  • Page 124 ACCQPrep HP150 Section 5 Maintenance 5.6 AutoSampler The Peristaltic pump on the back of the AutoSampler may need replacement. Maintenance Peristaltic Tubing Peristaltic tubing part number: SP7477. Replacement Figure 5-25 Back of the AutoSampler 1. Remove the outer band of the peristaltic pump (Figure 5-26).
  • Page 125 ACCQPrep HP150 Section 5 Maintenance Figure 5-27 Removing the tubing 3. Slide the new peristaltic tubing with the thicker tubing towards the AutoSampler (Figure 5-28). Figure 5-28 Attaching the new tubing 4. Replace the outer band of the peristaltic pump (Figure 5-29).
  • Page 126 ACCQPrep HP150 Section 5 Maintenance Figure 5-29 Replace the outer band 5.7 ELSD Maintenance Periodic cleaning of the spray chamber will keep the ACCQPrep operating at maximum performance. Follow the steps below if spray chamber cleaning is recommended: 1. From the R , open the M 5.7.1 Cleaning the ELSD...
  • Page 127 ACCQPrep HP150 Section 5 Maintenance tone. Lift the end of the tubing as needed to transfer most of the liquid into the spray chamber. Make sure the fluid level in the tubing doesn’t exceed the level of the instru- ment case top. If the tubing is raised too fast, fluid may flow out the top of the vent tube causing a spill.
  • Page 128 ACCQPrep HP150 Section 5 Maintenance 10. Allow the isopropyl alcohol to drain out of the unit by drop- ping the end of the P-trap drain line, while it is in the 100 mL beaker, below the P-trap drain line level.
  • Page 129 ACCQPrep HP150 Section 5 Maintenance 5.8 PurIon Maintenance Refer to Figure 5-33. 5.8.1 ESI and APCI Removal from PurIon S and 1. Place the mass spectrometer in S mode. TANDBY PurIon L 2. Unscrew the ¼-28 PEEK fitting at the top of the ion source housing.
  • Page 130 ACCQPrep HP150 Section 5 Maintenance 1. Place the ion source housing on top of the base plate and 5.8.4 Replacement of Ion line up with the rear electrical connection. Push down until Source Housing source chamber is seated evenly across the base plate.
  • Page 131 ACCQPrep HP150 Section 5 Maintenance Figure 5-34 Check valve holder 2. Remove check valves from pump head and place in a bea- ker of methanol. Sonicate check valves for 15 to 20 min- utes. 3. Reinstall check valves into pump head making sure that the ends of the check valves (with multiple holes) are fac- ing up towards the outlet of the pump.
  • Page 132 Even with the easy cleaning capability, Teledyne LABS recommends keeping a spare capillary inlet cone assembly (PN 25-0000-085) to allow rapid replacement to minimize downtime while cleaning the plugged capillary.
  • Page 133 ACCQPrep HP150 Section 5 Maintenance If the line cord is connected properly, check circuit breaker on the system’s rear panel to ensure it is switched to the O position. Table 5-1 Common PurIon Error Codes and Resolutions PurIon temperatures are stabilizing.
  • Page 134 ACCQPrep HP150 Section 5 Maintenance EAR99 Technology Subject to Restrictions Contained on the Cover Page 5-28...
  • Page 135 ACCQPrep HP150 Appendix A A.1 Diagrams for the The following diagrams are for connecting the AutoSampler to the ACCQPrep system. These diagrams include: AutoSampler and ACCQPrep • A tubing diagram of the connections from the AutoSampler to the ACCQPrep (Figure A-1) •...
  • Page 136 ACCQPrep HP150 Appendix A Figure A-1 Tubing diagram EAR99 Technology Subject to Restrictions Contained on the Cover Page...
  • Page 137 ACCQPrep HP150 Appendix A Figure A-2 Wiring diagram EAR99 Technology Subject to Restrictions Contained on the Cover Page...
  • Page 138 ACCQPrep HP150 Appendix A EAR99 Technology Subject to Restrictions Contained on the Cover Page...