Chapter 5 Basic Acquisition Methods; Create Acquisition Methods; Add An Experiment; Add A Period - AB Sciex 4500 Series System User's Manual

Basic Acquisition Methods
An acquisition method consists of experiments and periods. Use the Acquisition Method Editor to
create a sequence of periods and experiments for the instrument and devices.

Create Acquisition Methods

Only devices configured in the active hardware profile appear in the Acquisition Method Browser
pane. Any devices added to the hardware profile must also be added to existing acquisition
methods. For more information about devices, refer to the Peripheral Devices Setup Guide.
1. In the Navigation Bar, under Acquire, double-click Build Acquisition Method.
2. In the Acquisition Method Properties tab, select a Synchronization Mode.
3. Select the Auto-Equilibration check box. (Optional)
4. Enter the desired equilibration time in minutes, if Auto-Equilibration is selected.
5. In the Acquisition method pane, click the Mass Spec icon.
6. In the MS tab, select a scan type.
7. Type values in the fields as required. For more information refer to
page 46.
8. In the Advanced MS tab, type values in the fields as required. For more information
refer to Parameters on page
9. Click a device icon.
10. Select the parameters for the devices as required.
11. Add any additional periods and experiments.
12. Click File > Save.

Add an Experiment

1. Right-click the period and then click Add experiment.
An experiment is added below the last experiment in the period.
2. In the Acquisition Method Editor pane, select the appropriate device or instrument
parameters.

Add a Period

• In the Acquisition method pane, right-click the Mass Spec icon, and then click Add
period.
A period is added below the last period created.
System User Guide
Document Number: D5030863 A
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Parameters on
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