Show Dad Data; Generate Twcs - AB Sciex 4500 Series System User's Manual

Analyzing and Processing Data
Figure 7-3
3. Type start and stop values.
4. Click OK.
The XWC opens in a pane below the DAD spectrum.

Show DAD Data

The user can view DAD data in chromatogram or spectrum form, the same as mass
spectrometer data.
1. Open a data file containing data acquired with a DAD.
2. The TWC, which is analogous to a TIC, opens in a pane below the TIC.
3. In the TWC pane, click a point to select a single point in time, or highlight an area of
the spectrum to select a range of time.
4. Click Explore > Show > Show DAD Spectrum.
The DAD spectrum opens in a pane below the TWC. The y-axis shows absorbance
and the x-axis shows wavelength.
Tip!
to open it again. Click Explore > Show > Show DAD TWC.

Generate TWCs

A TWC shows total absorbance (mAU) on the y-axis plotted against time on the x-axis. For more
information about using the available icons, refer to
1. Open a data file that contains a DAD spectrum.
2. Click Explore > Show > Show DAD TWC.
The TWC opens in a pane below the DAD spectrum.
Tip!
click Show DAD TWC.
4500 Series of Instruments
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Extract Wavelengths dialog
If the pane with the TWC is closed, click a point anywhere in the TWC
Right-click inside the pane containing the DAD spectrum and then
Table 7-8 Graph Options on page
Document Number: D5030863 A
81.
System User Guide