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Hardware Guide 4000 QTRAP System ® D1000092200 B May 2010...
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This document is provided to customers who have purchased AB SCIEX equipment to use in the operation of such AB SCIEX equipment. This document is copyright protected and any reproduction of this document or any part of this document is strictly prohibited, except as AB SCIEX may authorize in writing.
The signal in the test sample is compared to the standard curve to determine the concentration. The typical scan type used for this application in the 4000 QTRAP system (as well as all triple quadrupole instruments) is MRM (Multiple Reaction Monitoring).
The typical scan type used for this application in the 4000 QTRAP system is an EMS (Enhanced MS) scan for intact molecular weight analysis. For more detailed structural analysis using tandem mass spectrometry, EPI (Enhanced Product Ion) and MS3 (MS/MS/MS) scans are performed.
DC voltages. The 4000 QTRAP system offers the following benefits over a standard triple quadrupole and a traditional 3-D ion trap mass spectrometer: •...
System Overview Turbo Pump Turbo Pump RF FEEDBACK RF FEEDBACK I/O Panel Gas and Vacuum Panel Figure 1-2 Back view of the instrument Component Descriptions This section contains general descriptions of the components of the instrument. Table 1-1 Component Descriptions Component Description I/O Panel...
Hardware Guide Curtain Gas™ flow LINAC® Collision Exit Cell Lens Deflector Skimmer Aux RF Orifice Curtain Plate Figure 1-3 Ion Optics Shutting Down and Turning on the System Use the following procedures if you need to shut down or turn on the system. To shut down the system 1.
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System Overview CAUTION! If the vacuum chamber is not going to be vented while the instrument is shut down, we recommend the roughing pump remain turned on to prevent the pump exhaust from being sucked back into the vacuum chamber. If you do not want to vent the vacuum chamber, skip steps 6 and 7.
Ammonium acetate (0 to 1%) Note: This list is not complete. If you are uncertain about a specific fluid, do not use the fluid until confirmation is received from AB SCIEX that it will not present a hazard. Source/Gas Parameters...
System Overview Compound Parameters In general, you can use the preset values for most of the parameters in Table 1-4. For more information about the parameters, see the Analyst software Help ® system. Table 1-4 Compound Parameters Parameter Value CAD Gas Use the preset value and optimize for your compound.
® installed automatically with the software and are available from the Start menu: All Programs > AB SCIEX. A complete list of the available documentation can be found in the online Help. To view the Analyst software Help, press F1.
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System Overview Document Number: D1000092200 B...
4000 Series Parameters The following tables contains generic parameters for the 4000 series of instruments. The first number under each scan type is the preset value; the range of numbers is the accessible range for each parameter. Table 2-1 4000 Series Instrument Parameters Positive Ion Mode Negative Ion Mode Parameter...
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Hardware Guide Table 2-2 4000 QTRAP System Parameters for LIT Scan Types Only ® Parameter ID Access ID Positive Ion Mode Negative Ion Mode High High Low–High Low–High AF2** 0 to 200 0 to 200 Mass-Speed Mass-Speed Dependent Dependent 0 to 5...
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4000 Series Parameters Document Number: D1000092200 B...