Verify Or Adjust Performance; Results Summary - AB Sciex 4500 Series System User's Manual

Instrument Tuning and Calibrating
iv. If available, click MS/MS/MS options and then click Next.
9. If the Select a mode page opens, select Automatic and then click Next.
10. Click GO.
The Verifying or Adjusting Performance screen opens. After the process has
completed, the Results Summary opens. For more information, refer to the Help.
11. Depending on the fields selected, the user will be prompted to change solutions for
the various scan types and polarities.

Verify or Adjust Performance

The top left corner shows the part of the instrument that is being tuned.
Current Spectrum: This graph shows the spectrum of the current scan, the optimal scan
selected by the software, or the scan at the current parameter value when the software results
are viewed in interactive mode.
The Instrument Optimization Decision Plots, in the top right graph, dynamically show the intensity
versus voltage curves of the parameters that are currently being optimized.

Results Summary

The Results Summary, shown in
were made by the Instrument Optimization software. This includes the location of data files and
instrument settings backups, as well as step-by-step changes and results during optimization. In
addition, the Results Summary shows a verification report. This report contains a snapshot of the
mass spectrum for each relevant mass for the scan modes being verified. The spectrum is
labelled with the target mass, where the mass was found, mass shift, peak width, and peak
intensity. The spectrum can be used as a visual record of peak shape or scan mode
performance. A summary table of results follows the spectra.
The Results Summary is saved as a document in the folder indicated at the top of the report.
Users can print the Results Summary or open a previously saved Results Summary.
4500 Series of Instruments
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Figure 4-1, is a record of any instrument settings changes that
System User Guide
Document Number: D5030863 A