Create A Results Table Using The Quantitation Wizard - AB Sciex 4500 Series System User's Manual

i. In the Name column, select reserpine.
ii. In the Internal Standard column, from the list, select the internal standard to
be associated with each analyte.
iii. In the Q1/Q3 column, select 609.400/195.039.
iv. If required, add one or more of the other compounds for a more complex
analysis.
Note: If the Compound ID field was populated for the samples and internal
standards in the acquisition method, then in the Analytes table, the Name
field and Q1/Q3 field are populated.
7. Click the Integration tab.
8. In general, the preset integration parameters are suitable for most peaks. If the
integration is not suitable, then change the algorithm.
9. Click the Show or Hide Parameters icon to show the additional integration
algorithms.
10. Click the Calibration tab. The preset parameters are suitable for these samples.
11. Save the quantitation method.
The new method can be used when a batch is created in the Batch Editor or when
the Quantitation Wizard is used to generate a Results Table.
Note: The quantitation method can only be used in the current project
unless it is copied to another project. To do this, click Tools > Project >
Copy Data. A new project must be created and selected to be available for
use.

Create a Results Table using the Quantitation Wizard

Prerequisites
• The Example project is selected.
• The Analyst Classic algorithm is used.
1. In the Navigation Bar, under Quantitate, double-click Quantitation Wizard.
The Create Quantitation Set - Select Samples page opens.
2. In the Available Data Files list, double-click the Triple Quad folder.
3. Select Mix_Batch_2.wiff.
4. Click Add All Files.
5. Click Next.
The Create Quantitation Set - Select Settings & Query page opens.
6. In the Default Query section, click Select Existing: Query.
7. In the Query list, select Accuracy 15%.
8. Click Next.
System User Guide
Document Number: D5030863 A
Analyzing and Processing Quantitative Data
4500 Series of Instruments
87 of 142