Show A Spectrum From A Tic; Generate Xics - AB Sciex 4500 Series System User's Manual

Analyzing and Processing Data

Show a Spectrum from a TIC

1. In a pane containing a TIC, select a range.
2. Click Explore > Show > Show Spectrum.
The spectrum opens in a new pane.
Tip!
spectrum.

Generate XICs

The user can generate XICs only from single period, single experiment chromatograms or
spectra. To obtain an XIC from multi-period or multi-experiment data, split the data into separate
panes by clicking the triangle that is under the x-axis. For more information about using the
available icons, refer to
There are several methods for extracting ions to generate an XIC, depending on whether
chromatographic or spectral data is used.
used with chromatograms and spectra.
Table 7-5 Summary of XIC Generation Methods
Method Use with
chromatogram
Selected range No
Maximum No
Base peak Yes
masses
Specified masses Yes
4500 Series of Instruments
74 of 142
Double-click in the TIC pane at a particular time to show the
Table 7-8 Graph Options on page
Table 7-5
Use with
spectrum
Yes
Yes
No
Yes
81.
contains a summary of methods that can be
Extraction
The selected range method extracts
ions from a selected area in a
spectrum.
The maximum method extracts ions
from a selected area in a spectrum
using the most intense peak in the
selected area. This creates an XIC
using the maximum mass from the
selected spectral range.
The base peak masses method can be
used only with BPCs (Base Peak
Chromatograms.) Use the Use Base
Peak Masses command to extract ions
results in an XIC with a different
colored trace for each mass. If the
selection includes multiple peaks, the
resulting XIC will have an equal
number of colored traces representing
each mass.
The specified masses method extracts
ions from any type of spectrum or
chromatogram. Select up to ten start
and stop masses for which to generate
XICs.
Document Number: D5030863 A
System User Guide