Table of Contents
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Table of Contents
Related Manuals for AB Sciex API 3200 System
Summary of Contents for AB Sciex API 3200 System
- Page 1 Hardware Guide API 3200 System ™ D1000092204 B May 2010...
- Page 2 This document is provided to customers who have purchased AB SCIEX equipment to use in the operation of such AB SCIEX equipment. This document is copyright protected and any reproduction of this document or any part of this document is strictly prohibited, except as AB SCIEX may authorize in writing.
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Page 3: Table Of Contents
Contents Chapter 1 System Overview ....... 5 Principles of the System ........6 Applications for the System . - Page 4 Contents Document Number: D1000092204 B...
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Page 5: Chapter 1 System Overview
System Overview The API 3200™ LC/MS/MS system includes a triple quadrupole mass spectrometer, a Turbo V™ ion source, a computer, and the Analyst software. ® WARNING! If you need to move the system, contact an FSE to assist you. Risk of personal injury or instrument damage. -
Page 6: Principles Of The System
Applications for the System The API 3200 system is designed for quantitative analysis of small molecules. This application involves measurement of specific molecular weight compounds, usually a drug or metabolite in a liquid sample, and their resulting fragment ions for determining the exact quantity of the compound in the sample of interest. -
Page 7: Parts Of The Instrument
CUR (Curtain Gas™ flow) Figure 1-2 Ion optics path The API 3200 system uses the Turbo V ion source to produce ions from liquid samples. The Turbo V source can use either the TurboIonSpray probe or the APCI (atmospheric pressure chemical ionization) probe. The instrument is configured to perform complex MS/MS analysis, but for less rigorous analytical requirements it can perform single MS scans. -
Page 8: Power Controls
System Overview Mass Spectrometer Ion Source CAD Gas Adjustment I/O Panel Valve AC Power CAD GAS SOURCES AUX I/O IEEE-4888 EXHAUST SUPPLY SHEATH SERIAL MIN 55 PSIG MAX 60 PSIG MAX 105 PSIG CURTAIN GAS Supply SUPPLY BACKING PUMP Max 60 PSIG MAX 60 PSIG EXHAUST WASTE OUT... -
Page 9: I/O Panel
Hardware Guide The Instrument Status lights indicate the status of the instrument vacuum. When the operational vacuum conditions are satisfied and the instrument is in analysis mode, the Ready light (green) is illuminated and the Fault light (red) is extinguished. The Fault light flashes when a vacuum fault is detected. In Pump- Down mode, the Ready light flashes for the duration of the sequence. -
Page 10: Gas Panel
System Overview • The sources port facilitates communication to optional ion sources, such as the control valve for the DuoSpray™ ion source, or provision of 24 VDC for the PhotoSpray ion source. ® • The AUX I/O outlet allows for communication with necessary peripheral devices, such as autosamplers or UV detectors. -
Page 11: Source Exhaust System
Hardware Guide EXHAUST SUPPLY SHEATH MIN 55 PSIG MAX 60 PSIG MAX 105 PSIG CURTAIN GAS SUPPLY MAX 60 PSIG MAX 60 PSIG EXHAUST WASTE OUT GAS 1 / GAS 2 MAX 105 PSIG VALVE WASTE OUT Gas Panel PP LY PS IG EX H M AX... -
Page 12: Vacuum Chamber
System Overview Ion Source Front Bulkhead Assembly Exhaust Supply Pressure Switch EXHAUST SUPPLY SHEATH MIN 55 PSIG MAX 60 PSIG MAX 105 PSIG CURTAIN GAS Venturi SUPPLY MAX 60 PSIG Pump MAX 60 PSIG EXHAUST WASTE OUT GAS 1 / GAS 2 Exhaust Waste Out MAX 105 PSIG VALVE... - Page 13 Hardware Guide CAUTION! Potential Instrument Damage: Shut off the sample flow before you shut down the instrument. 3. In the Analyst software, deactivate the hardware profile and then close the application software. 4. Stop the Analyst Service. (See the Software Reference Guide for the ®...
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Page 14: Instrument Safe Fluids
Ammonium acetate (0 to 1%) Note: This list is not complete. If you are uncertain about a specific fluid, do not use the fluid until confirmation is received from AB SCIEX that it will not present a hazard. Source/Gas Parameters... -
Page 15: Turbo V™ Ion Source Settings
Hardware Guide Table 1-1 Source/Gas Parameters Parameter Value Curtain Gas (CUR) IonSpray Voltage (IS) 5000 Temperature (TEM) Ion Source Gas (GS1) Ion Source Gas 2 (GS2) Turbo V™ Ion Source Settings Table 1-2 shows the recommended starting values for the X- and Y-axis parameters. -
Page 16: Related Documentation
The guides and tutorials for the instrument and the Analyst software are installed automatically with the software and are available from the Start menu: All Programs > AB SCIEX. A complete list of the available documentation can be found in the online Help. To view the Analyst software Help, press F1. -
Page 17: Chapter 2 3200 Series Parameters
3200 Series Parameters The following tables contain generic parameters for the 3200 series of instruments. The first number under each scan type is the preset value; the range of numbers is the accessible range for each parameter. Table 2-1 3200 Series Instrument Parameters Positive ion mode Negative ion mode Parameter... - Page 18 3200 Series Parameters Table 2-1 3200 Series Instrument Parameters (cont’d) Positive ion mode Negative ion mode Parameter Access MS/MS Q1 MS/MS Q0 +(–5) Q0 Q0 +(–5) Q0 + 5 Q0 + 5 Q0 + 5 +(–5) (ST = Q0 + offset) –8 to –2 –8 to –2 –8 to –2 2 to 8...
- Page 19 Hardware Guide Table 2-1 3200 Series Instrument Parameters (cont’d) Positive ion mode Negative ion mode Parameter Access MS/MS Q1 MS/MS –100 –100 –100 –400 to 0 –400 to –400 to 0 0 to 400 0 to 400 0 to 400 1800 1800 1800...
- Page 20 3200 Series Parameters Table 2-2 3200 QTRAP System Parameters For LIT Scan Types Only (cont’d) ® Parameter ID Access ID Positive ion mode Negative ion mode Mass-Speed Dependent Mass-Speed Dependent 0 to 5 0 to 5 Mass-Speed Dependent Mass-Speed Dependent –200 to 0 0 to 200 –400...