AB Sciex 4500 Series System User's Manual page 113

Typical values are between 30 psi and 50 psi for GS2.
Note: Gas 2 is used with higher flow rates typical with an LC system and in
conjunction with increased temperature.
3. Type a starting value for IonSpray Voltage (IS) or IonSpray Voltage Floating
(ISVF).
4. Adjust GS1 and GS2 in increments of 5 psi until the best signal or signal-to-noise
ratio is achieved.
5. In the Curtain Gas (CUR) field, type 20.
6. Increase CUR to the highest possible gas flow rate without a noticeable signal drop.
7. Adjust IS or ISVF in increments of 100 V to maximize signal to noise.
Optimize the Turbo Heater Temperature
The quantity and type of sample affects the optimal TurboIonSpray probe temperature. At higher
flow rates the optimal temperature increases. Normal optimization is usually performed in
increments of 50°C.
WARNING! Toxic Chemical Hazard: Vent the source exhaust system to an
external fume hood or an external vent to prevent hazardous vapors from being
released into the laboratory environment.
1. On the Source/Gas tab in the Tune Method Editor, type a starting value for
Temperature (TEM).
2. Wait for the signal to stabilize, to make sure that the ion source is at the correct
temperature.
3. Adjust the temperature parameter as required until the best signal or signal-to-noise
ratio is achieved.
Optimization Tips
• Use the highest temperature possible when optimizing compounds. A temperature
of 700°C is common for many compounds. High temperatures help keep the ion
source clean and reduce background noise.
• Use the highest Curtain Gas flow rate (CUR) possible without decreasing the signal.
This helps to:
• Prevent penetration of the Curtain Gas flow, which can produce a noisy signal
• Prevent contamination of the orifice
• Increase the overall signal-to-noise ratio
• Direct the liquid spray from the TurboIonSpray probe away from the orifice in order
to:
• Prevent contamination of the orifice
• Prevent piercing of the Curtain Gas flow, which can create an unstable signal
• Prevent electrical shorting due to the presence of the liquid
System User Guide
Document Number: D5030863 A
Turbo V™ Ion Source User Reference
4500 Series of Instruments
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