Chapter 8 Operating Instructions - Acquisition Methods; Create Acquisition Methods; Configure The Syringe Pump; Add An Experiment - AB Sciex QTRAP 4500 LC/MS/MS system System User's Manual

Operating Instructions — Acquisition
Methods
An acquisition method consists of experiments and periods. Use the Acquisition Method editor
to create a sequence of periods and experiments for the instrument and devices.

Create Acquisition Methods

Only devices configured in the active hardware profile appear in the Acquisition method pane.
Any devices added to the hardware profile must also be added to existing acquisition methods.
For more information about devices, refer to the Peripheral Devices Setup Guide.
1. In the navigation bar, under Acquire, double-click Build Acquisition Method.
2. In the Acquisition Method Properties tab, select a Synchronization Mode.
3. (Optional) Select the Auto-Equilibration check box and then type the desired
equilibration time, in minutes.
4. In the Acquisition method pane, click the Mass Spec icon.
5. On the MS tab, select a scan type.
6. Type values in the fields as required. Refer to
7. On the Advanced MS tab, type values in the fields as required. Refer to
on page 56.
8. Click a device icon and then select the parameters for the device. To configure the
syringe pump, refer to
9. Add any additional periods and experiments. Refer to
page 53 and
10. Click File > Save.

Configure the Syringe Pump

1. In the Acquisition method pane, click the Syringe Pump icon.
The Syringe Pump Method Properties tab opens in the Acquisition Method
editor pane.
2. In the Syringe Diameter (mm) field, type the syringe diameter.
3. In the Flow Rate field, type the flow rate.
4. In the Unit list, select the units of flow.

Add an Experiment

1. Right-click the period and then click Add experiment.
An experiment is added below the last experiment in the period.
2. In the Acquisition Method Editor pane, select the appropriate device or instrument
parameters.
System User Guide
RUO-IDV-05-0264-A
Configure the Syringe Pump on page
Add a Period on page 54
Parameters on page 56.
53.
Add an Experiment on
4500 Series of Instruments
8
Parameters
53 of 138