Probes - AB Sciex 4500 Series System User's Manual

Turbo V™ Ion Source User Reference
Figure A-1 Ion source components (Continued)
Item Description
2 Probe tower
3 X-axis adjustment knob used to position the probe on the horizontal axis for ion source
sensitivity adjustments
4 Grounding union
5 One of two source latches that secure the ion source to the mass spectrometer
6 Guide pin
7 Window port
8 Turbo heater
9 Bronze retaining ring
10 Y-axis adjustment knob used to position the probe on the vertical axis for ion source
sensitivity adjustments
11 Electrode adjustment nut
12 Sample tubing nut

Probes

Choose the probe and method most suitable for the compound in the sample stream flow.
Table A-1 Specifications of the Ion Source
Parameter
Ion source temperature
range
Liquid chromatography Interfaces to any liquid chromatography system
Nebulizer gas (Gas 1)
Heater gas (Gas 2)
The TurboIonSpray probe produces ions through ion evaporation. The APCI probe vaporizes the
sample before inducing ionization through atmospheric pressure chemical ionization. This
process is induced by a corona discharge needle as the ions pass through the ion source
housing to the interface region.
All of the data acquired using the ion source is identified with an abbreviation representing the
probe used to acquire the data (TIS for the TurboIonSpray probe, HN for the APCI probe).
®
TurboIonSpray Probe
The TurboIonSpray probe is suited for LC/MS/MS analyses. The sensitivity that is achieved with
this technique is dependent on both flow rate and analyte. At higher flow rates, ionization
efficiency increases, resulting in improved sensitivity. Compounds with extremely high polarity
and low surface activity usually show the greatest sensitivity increases. The TurboIonSpray
4500 Series of Instruments
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TurboIonSpray ® probe
Probe temperature from ambient
temperature to 750°C, depending on
liquid flow
Refer to the Site Planning Guide for the mass spectrometer.
APCI probe
Probe temperature from
50°C to 750°C,
depending on liquid flow
System User Guide
Document Number: D5030863 A