Temperature Control - Thermo Scientific thermoscientific Vanquish UHPLC System Operating Manual

Possible Cause
UV/Vis lamp defective
or not properly
installed
Piston calibration value
mismatch
8.2.8

Temperature Control

Column compartment temperature does not change although the set point has not been
reached
Possible Cause
Ambient temperature
too high or setpoint
lower than the
specified difference to
the ambient
temperature
Temperature control
turned off
Ventilation grid
obstructed
Ambient air enters
column chamber
Column compartment
defective
Pre-heater does not reach set temperature
Possible Cause
Pre-heater not
installed correctly
Pre-heater defective
UHPLC System
Operating Manual
Remedial Action
Check that the lamp is correctly installed. Replace the lamp (refer to Detector
Operating Manual).
Verify that the calibration value of the piston (imprinted on the rear of the piston)
corresponds to the related piston calibration value in the Chromeleon software.
Adapt the value in the Chromeleon software if necessary.
Remedial Action
Reduce the ambient temperature (for example, by ventilating the room).
Check the setting for TempCtrl in the Chromeleon software (TempCtrl=On) turns
temperature control on).
Make sure that the ventilation grids of the column compartment are not obstructed
in any way.
• Improper sealing of the column chamber may impair the heating and cooling
performance of the column compartment and lead to a considerable amount of
condensed water.
• When guiding capillaries through the column chamber cover seal, make sure that
the cover seals properly. If the chamber cover seal is damaged, replace the
chamber cover (refer to Column Compartment Operating Manual).
• Also, make sure that the front door is properly closed.
Contact Technical Support.
Remedial Action
The performance may be impaired if the thermal contact is imperfect, or if the pre-
heater inlet and outlet are exchanged. Reinstall the pre-heater (refer to Column
Compartment Operating Manual).
Replace the pre-heater (refer to Column Compartment Operating Manual).
8 • Troubleshooting
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