Thermo Scientific thermoscientific Vanquish UHPLC System Operating Manual page 98

6 • Operation
Interupting Operation of the System for a Longer Period
Page 98
To interrupt operation for a longer period, follow the instructions below.
Also observe the instructions and guidelines for the long-term shutdown
of the in the Operating Manual for each module.
1. Remove the column.
2. Flush the system with an appropriate solvent (minimum HPLC-
grade). Observe the following:
TIP With a Vanquish Core system that has been modified for using
normal-phase compatible solvents and additives, see the information
about the flushing liquid in the Considerations with Normal-Phase
Compatible Solvents and Additives section in Operating Manual for the
pump.
Situation after Shutdown
System remains in the
laboratory after shutdown
System shall be
transported or shipped
after shutdown
If no additive is used
Flush the system, for
example with methanol.
100% acetonitrile should
not be used.
Flush the system with
isopropanol.
If an additive is used
Flush the system with
several volumes of
methanol and water
(50:50) (for example,
1.0 mL/min for
10 minutes with the
standard system) to
prevent salt buildup in the
fluidics. If the solvents in
the device are not
miscible with water, use
an appropriate
intermediate solvent.
Flush the system first with
several volumes of
methanol and water
(50:50) (for example,
1.0 mL/min for
10 minutes with the
standard system) to
prevent salt buildup in the
fluidics. If the solvents in
the device are not
miscible with water, use
an appropriate
intermediate solvent.
Afterward, flush the
system with isopropanol.
UHPLC System
Operating Manual