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• Changes to run parameters will be ignored unless STEP RUN is deselected before START is
pressed.
• The only changes that could be made without deselecting would be if a different step run was
recalled from program memory (program numbers 10-15).
• After STEP RUN selections are completed, SAVE TO PROGRAM MEMORY? will appear in the
SET DISPLAY.
SAVE TO PROGRAM MEMORY?
• SAVE RUN allows pre-programming of up to 15 different sets of run parameters (9 standard runs,
and 6 step runs) for future simple recall and error-free run reproducibility.
• In addition to basic parameters, all option selections and settings (excluding rotor name and
overtemperature limit) will also be saved to program memory.
• To eliminate inadvertent loss of existing programs, the save run sequence alerts users before
overwriting.
• The RECALL key allows browsing through existing programs for selection or reference.
Note If you plan to use the QC RUN feature, specific guidelines should be considered when
saving parameters to memory.
• To save a run, all run parameter selections should be made before selecting this option.
• After SAVE RUN selection and assignment of a program number, the SET DISPLAY will return
to the HOME screen.
Temperature Control
The centrifuge controls calculated sample temperature during a run based on the rotor selected, rotor
speed, run time, SET temperature and measured chamber temperature. The complex calculation the
centrifuge performs must assume, however, that the sample and the rotor are at SET temperature at the
start of the run. If temperatures are not equilibrated, adjustments made by the centrifuge's control
system may not be appropriate (although, over time, temperature control should come into range).
Note When temperature control is critical, the sample, SET temperature, rotor (body, buckets,
adapters, and cover), and rotor chamber should all be at the same temperature when START is
pressed. Also, remove the sample soon after a run has ended, so that it will not be affected if
chamber temperature drops slightly when the rotor is no longer generating heat due to air friction.
Either store the rotor in a controlled temperature environment (such as refrigerator or cold room), or
precool/preheat the rotor in the centrifuge chamber, until the rotor is the same temperature as the
sample and the required SET temperature.
Temperature control needs can vary with the application. In many cases, the centrifuge will be used to
run large volumes of sample for short amounts of time. With such a run, if all components have been
equilibrated, it would be difficult to significantly change sample temperature during the run - even if
the centrifuge's temperature control performance was reduced.
Thermo Scientific
4
Operation
Temperature Control
RC 12BP+
4-13
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