Thermo Scientific TX-750 Instruction Manual
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Thermo Scientific TX-750 Instruction Manual

Thermo Scientific TX-750 Instruction Manual

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Thermo Fisher Scientific
TX-750
Instruction Manual
50119938-4
October 2009

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Summary of Contents for Thermo Scientific TX-750

  • Page 1 Thermo Fisher Scientific TX-750 Instruction Manual 50119938-4 October 2009...
  • Page 2 © 2009 Thermo Fisher Scientific Inc. All rights reserved. AutoLock is either a registered trademark or a trademark of Thermo Fisher Scientific. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the product operation.

  • Page 5: Table Of Contents

    Sorvall 100V 50/60Hz Round bucket 75003608 ..........1-10 Sorvall 100V 50/60Hz rectangular bucket 75003614 .........1-11 Thermo Scientific ....................1-12 Thermo Scientific 230V 50Hz/60Hz Round bucket 75003608 ......1-12 Thermo Scientific 230V 50Hz/60Hz rectangular bucket 75003614 ....1-13 Thermo Scientific 120V 60Hz Round bucket 75003608 ........1-14 Thermo Scientific 120V 60Hz rectangular bucket 75003614 ......1-15...
  • Page 6 Decontamination ..................... 6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-6 RCF-Values .......................A-1 Rectangular buckets 75003614 ................A-1 Round bucket 75003608 ..................A-2 Chemical Compatibility Chart ................B-1 Warranty and recommended Service Life ............C-1 Contact Information ....................D-1 75003607 TX-750 Thermo Scientific...

  • Page 7: Preface

    If any parts are missing, please contact the nearest Thermo Fisher Scientific representative. Precautions In order to ensure safe operation of the TX-750, the following general safety regulations must be followed: • Do not remove the magnet at the rotor bottom •...
  • Page 8 ATTENTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. 75003607 TX-750 Thermo Scientific...
  • Page 9 Rotor Specifications Contents “Heraeus” page 1-2 • “Sorvall” page 1-6 • “Thermo Scientific” page 1-12 • Thermo Scientific 75003607 TX-750...

  • Page 10: Chapter 1 Rotor Specifications

    19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 50 / 55 50 / 55 50 / 55 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...

  • Page 11: 4X750Heraeus 230V 50Hz/60Hz Rectangular Bucket 75003614

    19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 40 / 50 40 / 50 40 / 50 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...

  • Page 12: Heraeus 120V 60Hz Round Bucket 75003608

    19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 75 / 60 75 / 60 75 / 60 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...

  • Page 13: Heraeus 120V 60Hz Rectangular Bucket 75003614

    19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 65 / 50 65 / 50 65 / 50 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...

  • Page 14: Sorvall

    19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 50 / 55 50 / 55 50 / 55 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...

  • Page 15: Sorvall 230V 50Hz/60Hz Rectangular Bucket 75003614

    19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 40 / 50 40 / 50 40 / 50 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...

  • Page 16: Sorvall 120V 60Hz Round Bucket 75003608

    19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 75 / 60 75 / 60 75 / 60 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 75003607 TX-750 Thermo Scientific...

  • Page 17: Sorvall 120V 60Hz Rectangular Bucket 75003614

    19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 65 / 50 65 / 50 65 / 50 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750...

  • Page 18: Sorvall 100V 50/60Hz Round Bucket 75003608

    19.5 / 8.3 19.5 / 8.3 19.5 / 8.3 Pitch angle [°] Accel. / braking time [s] 80 / 60 80 / 60 80 / 60 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK 1-10 75003607 TX-750 Thermo Scientific...

  • Page 19: Sorvall 100V 50/60Hz Rectangular Bucket 75003614

    19.5 / 8.9 19.5 / 8.9 19.5 / 8.9 Pitch angle [°] Accel. / braking time [s] 65 / 50 65 / 50 65 / 50 aerosol-tight permissible temperature range autoclavable °C Tested by HPA, Porton-down, UK Thermo Scientific 75003607 TX-750 1-11...

  • Page 20: Thermo Scientific

    Rotor Specifications Thermo Scientific Thermo Scientific Table 1-11. Thermo Scientific 230V 50Hz/60Hz Round bucket 75003608 Centrifuge Thermo Scientific SL 40 Thermo Scientific SL 40 F Catalog # 75004512 75004542 Weight empty [kg] max. cycle number 50,000 50,000 Maximum permissible load [g]...

  • Page 21: Thermo Scientific 230V 50Hz/60Hz Rectangular Bucket 75003614

    Rotor Specifications Thermo Scientific Table 1-12. Thermo Scientific 230V 50Hz/60Hz rectangular bucket 75003614 Centrifuge Thermo Scientific SL 40 Thermo Scientific SL 40 F Catalog # 75004512 75004542 Weight empty [kg] max. cycle number 50,000 50,000 Maximum permissible load [g] 4x750...

  • Page 22: Thermo Scientific 120V 60Hz Round Bucket 75003608

    Rotor Specifications Thermo Scientific Table 1-13. Thermo Scientific 120V 60Hz Round bucket 75003608 Centrifuge Thermo Scientific SL 40 Catalog # 75004513 Weight empty [kg] max. cycle number 50,000 Maximum permissible load [g] 4x800 maximum speed n [rpm] 4,700 maximum RCF value at n 4,816 max.

  • Page 23: Thermo Scientific 120V 60Hz Rectangular Bucket 75003614

    Rotor Specifications Thermo Scientific Table 1-14. Thermo Scientific 120V 60Hz rectangular bucket 75003614 Centrifuge Thermo Scientific SL 40 Catalog # 75004513 Weight empty [kg] max. cycle number 50,000 Maximum permissible load [g] 4x750 maximum speed n [rpm] 4,300 maximum RCF value at n 4,031 max.
  • Page 24 Accessories Contents „Rotor data“ on page 2-2 • „Accessories for round bucket“ on page 2-2 • „Rotor data“ on page 2-4 • „Accessories for rectangular bucket“ on page 2-4 • Thermo Scientific 75003607 TX-750...
  • Page 25 Accessories Thermo Scientific General Purpose Rotors TX-750 Round Buckets TX-750 Swinging Bucket Aluminum Rotor with Round Buckets Rotor Specifications Capacity (ml) 4 x 750 Tube Dimensions (mm) 98 x 133 Angle Maximum Speed (rpm) 4.700 K Factor 9783 Net Weight (kg)

  • Page 26: Chapter 2 Accessories

    Accessories Thermo Scientific General Purpose Rotors -750 Round Buckets (cont'd) Tube Ordering Information Sealing Assembly Required: Adapters Required: Accessories Required: Tube Vol. Max. Dims ØxL Cat. No. Vol. Fill Description Qty. Speed No. of (mm) Cat. No. Description Cat. No.
  • Page 27 Accessories Thermo Scientific General Purpose Rotors TX-750 Rectangular Buckets TX-750 Swinging Bucket Aluminum Rotor with Rectangular Buckets Rotor Specifications Capacity (ml) 4 x 250 Tube Dimensions (mm) 63 x 130 Angle Maximum Speed (rpm) K Factor 8,983 Net Weight (kg)
  • Page 28 Accessories Thermo Scientific General Purpose Rotors TX-750 Rectangular Buckets (cont'd) Tube Ordering Information Sealing Assembly Required: Adapters Required: Accessories Required: Tube Vol. Max. Dims ØxL Cat. No. Vol. Fill Description Qty. Speed No. of (mm) Cat. No. Description Cat. No.

  • Page 29: Autolock Tm

    AutoLock Contents “Rotor Installation” page 3-2 • “Removing the Rotor” page 3-3 • Thermo Scientific 75003607 TX-750...

  • Page 30: Rotor Installation

    WARNING If the rotor cannot be properly locked in place after several attempts, then the AutoLock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor, damaged rotors must not be used. Keep the hub area clear of objects. 75003607 TX-750 Thermo Scientific...

  • Page 31: Removing The Rotor

    2. Grab the rotor handle with both hands and press against the green AutoLock button. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to jam the rotor while doing this. Figure 4. Handling of AutoLock Thermo Scientific 75003607 TX-750...
  • Page 32 Rotor Loading Contents “Before a Run” page 4-2 • “Proper Loading” page 4-2 • “Improper Loading” page 4-3 • “Maximum Loading” page 4-3 • “Cycle Counter” page 4-4 • Thermo Scientific 75003607 TX-750...

  • Page 33: Chapter 4 Rotor Loading

    To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times. You can use two rectangular buckets or two round buckets or two microplate carriers. Make sure that the opposing receptacles are holding tubes of the same weight. 75003607 TX-750 Thermo Scientific...

  • Page 34: Improper Loading

    There are two options available for centrifuging samples whose weight, including adaptor, exceeds the maximum permissible load: • Reduce the fill level. Reduce the speed. Use the table or the formula: n max Maximum permissible load n adm --------------------------------------------------------------------- - Effective load = admissible speed = maximum speed Thermo Scientific 75003607 TX-750...

  • Page 35: Cycle Counter

    Replace the buckets when the specified number of cycles is reached. Service life examples Usage profile Maximum service life at 50,000 cycles frequent use 7 years 20 runs / day 220 days / year 75003607 TX-750 Thermo Scientific...

  • Page 36: Aerosol-Tight Applications

    Aerosol-tight Applications Contents “Basic Principles” page 5-2 • “Fill level” page 5-3 • “Checking the aerosol Tightness” page 5-2 • Thermo Scientific 75003607 TX-750...

  • Page 37: Basic Principles

    This releases the carbonic acid gas which is bound in the water, resulting in excess pressure. Do not apply pressure to the lid when doing so! Leaks can be detected by escaping water or the sound of escaping gas. 75003607 TX-750 Thermo Scientific...
  • Page 38 Bucket with closed lid CAUTION If the latch is not flipped down, the caps could be damaged during centrifuging. If the latch has not clicked into place, the bucket is not aerosol-tight. Never raise the bucket at its latch. Thermo Scientific 75003607 TX-750...
  • Page 39 Maintenance and Care Contents “Cleaning Intervalls” page 6-2 • “Cleaning” page 6-2 • “Disinfection” page 6-3 • “Decontamination” page 6-4 • “Autoclaving” page 6-5 • “Service of Thermo Fisher Scientific” page 6-6 • Thermo Scientific 75003607 TX-750...

  • Page 40: Chapter 6 Maintenance And Care

    • Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. 75003607 TX-750 Thermo Scientific...

  • Page 41: Disinfection

    CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific 75003607 TX-750...

  • Page 42: Decontamination

    For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and water. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 75003607 TX-750 Thermo Scientific...

  • Page 43: Autoclaving

    Place the rotor on a flat surface. Note No chemical additives are permitted in the steam. CAUTION Never exceed the permitted temperature and duration when autoclaving. If the rotor shows signs of corrosion or wear, it must be replaced. Thermo Scientific 75003607 TX-750...

  • Page 44: Service Of Thermo Fisher Scientific

    • the suitability of the set-up site • the lid lock and the safety system • the rotor • the fixation of the rotor and the drive shaft Thermo Fisher Scientific offers inspection and service contracts for this work. 75003607 TX-750 Thermo Scientific...

  • Page 45: Rcf-Values

    1600 19.5 1700 19.5 1800 19.5 1900 19.5 2000 19.5 2100 19.5 2200 19.5 1055 2300 19.5 1153 2400 19.5 1256 2500 19.5 1363 2600 19.5 1474 2700 19.5 1589 2800 19.5 1709 2900 19.5 1833 Thermo Scientific 75003607 TX-750...

  • Page 46: Round Bucket 75003608

    Table A-2.Round bucket 75003608 Speed (rpm) Min. radius Max. radius RCF Rmin RCF Rmax 19.5 19.5 19.5 19.5 19.5 19.5 19.5 1000 19.5 1100 19.5 1200 19.5 1300 19.5 1400 19.5 1500 19.5 1600 19.5 1700 19.5 1800 19.5 75003607 TX-750 Thermo Scientific...
  • Page 47 3900 19.5 1411 3316 4000 19.5 1485 3488 4100 19.5 1560 3665 4200 19.5 1637 3846 4300 19.5 1716 4031 4400 19.5 1796 4221 4500 19.5 1879 4415 4600 19.5 1964 4613 4700 19.5 2050 4816 Thermo Scientific 75003607 TX-750...

  • Page 48: Chemical Compatibility Chart

    S U U U U U U U - M S M - Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S Thermo Scientific 75003607 TX-750...
  • Page 49 S S S U S U S S S U ® Ficoll-Hypaque M S S - S S S - S S S S - S S - S S S S S S S M S S S 75003607 TX-750 Thermo Scientific...
  • Page 50 S S S U S S M S S S S S U S U M S S S S S S S M S M U Methylene Chloride U U U U M S S U S U U S U U U U U M U U U S S M U S U Thermo Scientific 75003607 TX-750...
  • Page 51 S S S S S S S S S S S S S S S S S S S Picric Acid S S U - S M S S S M S U S S S U S S S S U S U M S M S 75003607 TX-750 Thermo Scientific...
  • Page 52 S S S S S S S S S S S S S S S S S S S S S S S Citric Acid (10%) M S S M S S M S S S S S S S S S M S S S S S S S S S S Thermo Scientific 75003607 TX-750...
  • Page 53 Performance unknown; suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. Because no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. 75003607 TX-750 Thermo Scientific...

  • Page 54: Contact Information

    +49 6184 90 6940 France +33 2 2803 2180 +33 2 2803 2000 Italy +39 02 02 95059 341 Netherlands +31 76 571 4440 Nordic / Baltic Countries +35 89 329 100 Russia +7 (812) 703 42 15 Thermo Scientific 75003607 TX-750...
  • Page 55 +86 10 8419 3588 India +91 22 6716 2200 Japan +81 45 453 9220 Other Asian Countries +852 2885 4613 Latin America +1 866 984 3766 Other Countries +49 6184 90 6940 +33 2 2803 2180 75003607 TX-750 Thermo Scientific...
  • Page 56 Cleaning Intervalls .......... 6-2 Service Life ............. C-1 CLICKSeal ............5-3 Sorvall .............1-6 Contact Information ........D-1 Cycle Counter ..........4-5 Thermo Scientific ..........1-12 Decontamination ..........6-4 Disinfection ............ 6-3 Warranty ............C-1 Fill Level ............5-2 Heraeus ............1-2 Improper Loading ........... 4-3 Maintenance ...........

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