Peak Area Precision - Thermo Scientific thermoscientific Vanquish UHPLC System Operating Manual

Possible Cause
Electrical interferences
from other instruments
Column temperature
significantly above
boiling point of mobile
phase
8.2.3

Peak Area Precision

Contamination or carry-over
Possible Cause
Contamination in the
system
Autosampler carry-over
Environmental conditions
Possible Cause
Unstable
environmental
conditions
Capillary problems
Possible Cause
Capillary connections
not installed properly
or not tight, dead
volumes in capillary
connections
UHPLC System
Operating Manual
Remedial Action
Avoid operation of equipment generating strong electric or magnetic fields in close
proximity. Do not connect equipment that may cause power disturbances to the
same power network used to supply the system.
• Install a restrictor at the flow cell outlet, observing the pressure specification of
the respective flow cell and the general guidelines for the flow cell.
Note: Excessive backpressure will destroy flow cells!
• Install a post-column cooler (refer to Column Compartment Operating Manual).
Remedial Action
Flush the system using an appropriate solvent.
The needle may be contaminated. Wash the needle in the wash port. If carry-over
persists, replace the needle seat (refer to Split Sampler Operating Manual).
Remedial Action
Make sure that the temperature and air humidity are constant. Use column
thermostatting. Avoid draft. Use insulation on the capillary from the column
compartment to the detector.
Remedial Action
Properly install the capillary connections. Tighten the capillary connections. Replace
capillary.
8 • Troubleshooting
Page 117