Priming The Sample Manager - Waters ACQUITY UPLC Overview And Maintenance Manual

Table 2–2: Wash solvent effects (continued)
Property
Sample diluent
Cycle times

2.14 Priming the sample manager

Priming fills the wash system with wash solvent or the injection pathway with purge solvent. You
prime the system to accomplish these tasks:
• Preparing a new sample manager for operation.
• Preparing a sample manager for operation after it has been idle for more than 24 hours.
• Preparing the purge solvent.
• Changing the wash solvent.
• Removing bubbles from the lines.
Ensure that the purge and wash solvents are correctly composed, that they are LC/MS-grade,
and that they are miscible with other solvents used in the system. Use filters in all solvent
reservoirs, and ensure that the volumes of solvents suffice for priming.
To prime the sample syringe and wash solvent:
1. From the system view of the ACQUITY UPLC Console, select the sample manager, and
then click Control > Prime .
Alternative:
click and then click Prime.
2. In the Prime dialog box, click the boxes on the left-hand side to select a priming function.
3. Specify a duration, in seconds, for priming the wash solvent and the number of cycles for
priming the purge solvent, and then click OK.
Table 2–3: Priming parameter values
Parameter
Priming range
Default priming
Recommended priming: dry inlet tube
Effect
The purge solvent (diluent) will contact the sample, so match the sample
matrix as closely as possible. To offset adverse effects on peak shape
caused by the matrix's composition, adjust the purge solvent composition.
Higher viscosity wash solvents lengthen wash cycles.
In the data application, in the control panel of the sample manager, right-
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Wash solvent Purge solvent
1 to 600 seconds 1 to 100 cycles
15 seconds 5 cycles
180 seconds 100-µL syringe: 60 cycles
250-µL syringe: 24 cycles
500-µL syringe: 12 cycles