Sample Chamber Considerations; Choosing Sample Needles; Choosing Extension Loops - Waters ACQUITY UPLC Overview And Maintenance Manual

• Use cap-mats on sample plates whenever possible.
• Use pre-slit cap mats/seals and vial caps. Use of non-pre-slit cap mats and vial caps can
cause clogging in the wash drainage lines.
• To prevent sample spillage or needle damage, use only Waters-approved covers on the
sample vials.
See also:
For more information about plates and vials, see the Waters Sample Vials and
Accessories Brochure (720001818EN) or visit the
website.

2.18 Sample chamber considerations

Warning:
needle assembly mechanism while it is moving. Note that the sample manager beeps
three times whenever the sample compartment door is open, and the needle assembly
mechanism is about to move.

2.19 Choosing sample needles

Waters offers multiple needle sizes, so you can choose the best option for your injection volume
and sample viscosity. The 15-µL needle provides the best injection volume accuracy, while the
30-µL needle allows for sample aspiration rates up to four times faster.
Tip:
Using a smaller needle increases cycle times.
Table 2–5: Supported sample needles for the sample manager
Needle size
15-µL – Default
30-µL

2.20 Choosing extension loops

Extension loops, which increase the volume of sample that can be drawn and held for injection,
are an optional part of the injection system. You install them between the needle and the
injection-valve port. The following extension loop sizes are supported:
• 50 µL (default)
• 100 µL
To avoid puncture wounds, keep hands and loose clothing clear of the
Recommended maximum injection volume
10 µL
25 µL
June 11, 2019, 715005051 Rev. D
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plate selector and vial selector on the Waters