Waters ACQUITY UPLC Overview And Maintenance Manual page 124

Item Specification
Sample carryover - <0.005% sulphadimethoxine (MS)
MS Test conditions:
• Solvent A: water with 0.1% formic acid
• Solvent B: acetonitrile with 0.1% formic acid
• Weak wash: 5:95 acetonitrile/water
• Strong wash: 50:50 acetonitrile/water
• Mobile phase: 80% solvent A:20% solvent B
• Flow rate: 0.3 mL/min
• Sample: sulphadimethoxine at 5 pg/µL (standard) and 1 µg/µL
• Injection volume: 5 µL
• Column: ACQUITY UPLC BEH C
• Column temperature: 40 °C
• Sample temperature: 10 °C
• Detection: MS SIR at 311.3 Da, 0.5 s dwell or MRM at 156.0 to 310.0
• Ion mode: ES+
• Run time: 5 min
• Data system: Empower or MassLynx software
Basis of calculation: Any peaks in the blanks following the challenge
sample are compared with the known (0.005%) standard. Carryover peak
areas <80% of the standard area are within specification.
(challenge) in 10:90 acetonitrile/water +0.1% formic acid, compared
with blanks of 10:90 acetonitrile/water +0.1% formic acid
June 11, 2019, 715005051 Rev. D
Page 124
1.7 µm, 2.1 × 50 mm
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