Waters ACQUITY UPLC Overview And Maintenance Manual page 121

Item Specification
Accuracy ±0.2 µL, measured by fluid weight removed from vial by means of 10-µL
(aspiration) injections, averaged over 20 injections, using a 100-µL syringe.
Linearity >0.999 (standard needle)
Test conditions:
• Chromatography: isocratic
• Mobile phase: 10:90 acetonitrile/water
• Flow rate: 0.6 mL/min
• Needle volume: 1 to 70%
• Sample mix: caffeine 0.03 mg/mL (0.2 to 10.0 µL, with 15 µL needle
• Column: ACQUITY UPLC BEH C
• Detector: UV, 273-nm wavelength
• Column temperature: 40 °C ±0.3 °C
Precision <1% area RSD, 0.2 to 1.9 µL (0.25 to 0.50 mg/mL caffeine),
<0.5% area RSD, 2.0 to 10.0 µL (0.03 mg/mL caffeine)
Test conditions:
• Chromatography: isocratic
• Replicates: 6
• Mobile phase: 10:90 acetonitrile/water
• Flow rate: 0.6 mL/min
• Column: ACQUITY UPLC BEH C
• Detector: UV, 273-nm wavelength
• Column temperature: 40 °C ±0.3 °C
Number of sample Any two of the following Waters-certified plates:
plates
• 96 and 384 microtiter plates
• 48-position, 2.00-mL vial plates
• 48-position, 0.65-mL micro-centrifuge tube plates
• 24-position, 1.50-mL micro-centrifuge tube plates
For more information, see the Waters Sample Vials and Accessories
Brochure (720001818EN) or visit the
Waters website.
installed, no extension loop)
June 11, 2019, 715005051 Rev. D
Page 121
, 1.7 µm, 2.1 × 50 mm
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, 1.7 µm, 2.1 × 50 mm
18
plate selector and vial selector on the