Waters ACQUITY UPLC Overview And Maintenance Manual page 97

26. Using a rounded tool (such as the shaft of the T20 TORX driver), make a bend and
connect the needle tubing to the extension-loop port or, if the extension loop is not
installed, to port 4 on the injection valve.
Figure 3–52: Needle tubing installed on injection valve
Needle tubing
27. Ensure that the needle tubing is fully inserted into port 4 on the injection valve, and then
thread the fitting into the port.
28. While holding the needle tubing against the bottom of the port, finger-tighten the
compression screw, and then use the 1/4-inch open-end wrench to tighten an additional
3/4-turn.
Note:
Failure to fully bottom the needle tubing and ferrule could cause carryover and poor
chromatography.
See also:
If you are using an extension loop, refer to
properly connect to port 4.
29. Close the sample compartment door and the fluidics compartment door.
30. Calibrate the needle (see the console online Help).
Recommendation:
31. Characterize the needle seal (see the console online Help).
32. Perform the needle-seal readiness test (see the console online Help).
33. Click Reset.
Result:
The sample manager returns to normal operation.
34. Specify the needle volume in the Volume configuration dialog box, if it changed.
Replace the needle seal whenever you replace the sample needle.
June 11, 2019, 715005051 Rev. D
Page 97
Installing extension loops to