Audience And Purpose; Intended Use Of The Acquity Refractive Index Detector; Calibrating; Quality-Control - Waters ACQUITY Refractive Index Detector Overview And Maintenance Manual

Audience and purpose

This guide is intended for use by individuals who operate and maintain the
ACQUITY refractive index detector.

Intended use of the ACQUITY refractive index detector

Waters designed the ACQUITY Refractive Index Detector for use in
ultra-performance liquid chromatography and advanced polymer
chromatography applications. It provides sensitivity, stability, and
reproducibility for the analysis of components, with limited or no UV
absorption. The refractive index detector is for research use only and is not
intended for use in diagnostic applications.

Calibrating

To calibrate LC systems, follow acceptable calibration methods using at least
five standards to generate a standard curve. The concentration range for
standards should include the entire range of QC samples, typical specimens,
and atypical specimens.

Quality-control

Routinely run three QC samples that represent subnormal, normal, and
above-normal levels of a compound. Ensure that QC sample results fall within
an acceptable range, and evaluate precision from day to day and run to run.
Data collected when QC samples are out of range might not be valid. Do not
report these data until you are certain that the instrument performs
satisfactorily.
October 13, 2014, 715003547 Rev. C vii