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4 Maintenance Procedures
•
HPLC-grade water
•
A strong cleaning solvent suitable for the system and application (6N
nitric acid is used frequently)
•
A separate waste container for acid waste
•
If you use an acid as a cleaning solvent, a means of measuring the pH of
the acid effluent
Caution:
sensitivities, flush the system extensively with water to remove all
traces of the acid.
To decontaminate the fluid path:
Strictly adhere to the following procedure.
Note:
1.
Stop the solvent manager, and replace the column with a union.
2.
Replace the mobile phase with an intermediary solvent miscible in both
the current solvent and water.
3.
Restart the solvent manager, and then set the flow rate to 1 mL/min,
maintaining the purge for at least 10 minutes.
See also:
ACQUITY Console online Help.
4.
Set the detector to purge mode for approximately 10 minutes, and run
for an additional 10 minutes in normal mode.
5.
Switch the solvent manager to HPLC-grade water, and flush the
detector for 10 minutes.
Recommendation:
cleaning solution. Do not allow organic waste to mix with acid waste.
6.
Switch the solvent manager to the cleaning solvent, and flush the
detector for 10 minutes.
7.
Switch the solvent manager back to HPLC-grade water and flush until
the pH of the waste effluent is neutral (a pH value of 6.0 to 7.0).
8.
Switch the solvent manager back to the water-miscible intermediate
solvent, and flush for 10 minutes.
78
October 13, 2014, 715003547 Rev. C
If you use 6N nitric acid and operate the detector at high
For information about purging the detector, consult the
Provide a separate, clean, waste container for the
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