Solvent Degassing; Gas Solubility - Waters ACQUITY Refractive Index Detector Overview And Maintenance Manual

2 Preparing the detector
Refractive indices of common solvents: (Continued)
Solvent
Isopropyl chloride
Isopropanol
n-Propanol
Methylethylketone
Diethyl amine
n-Propyl chloride
Methylisobutylketone
Nitromethane
1-Nitropropane
Isooctane
Cyclopentane

Solvent degassing

Using degassed solvents is the most important step in solvent preparation.
Degassing provides these benefits:
• Stable baselines and enhanced sensitivity
• Reproducible retention times
• Stable pump or solvent delivery system operation

Gas solubility

The amount of gas that can dissolve in a given volume of liquid depends on
these factors:
• The chemical affinity of the gas for the liquid
• The temperature of the liquid
• The pressure applied to the liquid
Changes in the composition, temperature, or pressure of the mobile phase can
lead to changes in dissolved gas content.
52 October 13, 2014, 715003547 Rev. C
RI Solvent
1.378 Ethylene dichloride
1.38 Carbon tetrachloride
1.38 Dimethyl sulfoxide
(DMSO)
1.381 Toluene
1.387 Xylene
1.389 Benzene
1.394 Pyridine
1.394 Chlorobenzene
1.400 o-Chlorophenol
1.404 Aniline
1.406 Carbon disulfide
RI
1.445
1.466
1.477
1.496
~1.50
1.501
1.510
1.525
1.547
1.586
1.626