Bubble Trap - Thermo Scientific Gibco CTS Rotea User Manual

Bubble Trap

To manage air in the system, the CTS
capacity of approximately 5 mL.
1
Figure 9 CTS ™ Rotea
™ ™
CTS Rotea
1
Close-up of Bubble Trap
2
CAUTION! Once the Bubble Trap volume is consumed, any additional air in the system will now
be able to enter the CFC Chamber and ultimately cause an over-pressure fault, shutting down the
instrument.
CAUTION! Protocols created for the Rotea
to ensure air in fluid supply lines and the Bubble Trap is replaced with fluid prior to commencing
processing.
Note: If the length of tubing from the product supply bag has a volume greater than the Bubble Trap,
then air can enter the circuit. It is therefore preferred that the length of tubing for each connection (1/8"
ID assumed) is less than 12" (or 300 mm). In some cases where the supply vessel is large, e.g. remote
bioreactor, additional process strategies can be included to re-fill the supply tube when needed.
CTS ™ Rotea ™ Counterflow Centrifugation System User Guide
™
Rotea
™ Single-Use Kit and close-up of Bubble Trap
Single-Use Kit
Chapter 3 Basic instrument operation
™
Single-Use Kit includes a small bubble trap with a
2
™
instrument must include a suitable priming sequence
Kit preparation, loading and removal
3
47