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Ion Torrent A45727
Thermo Scientific Ion Torrent A45727 Manuals
Manuals and User Guides for Thermo Scientific Ion Torrent A45727. We have
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Thermo Scientific Ion Torrent A45727 manual available for free PDF download: User Manual
Thermo Scientific Ion Torrent A45727 User Manual (163 pages)
Integrated Sequencer
Brand:
Thermo Scientific
| Category:
Laboratory Equipment
| Size: 7.79 MB
Table of Contents
Table of Contents
3
CHAPTER 1 Product Information
8
Product Description
8
Genexus ™ Integrated Sequencer
9
Library Chemistry
10
Genexus Library Strips 1 and 2-AS
10
Genexus ™ Barcodes as
10
Reagents and Supplies-Ion Ampliseq
10
Genexus GX5 Starter Pack-AS
11
HD Library Chemistry
12
Genexus Library Strips 1 and 2-HD
12
Genexus ™ Barcodes 1-32 HD
12
Reagents and Supplies-Ion Ampliseq
12
Genexus GX5 Starter Pack-HD
13
Shared Reagents and Supplies
14
Genexus Templating Strips 3 and 4-GX5
14
GX5 Chip and Genexus Coupler
15
Genexus ™ Sequencing Kit
15
Genexus ™ Controls
16
Genexus ™ Filter
16
Oncomine ™ GX Assays
16
Required Materials Not Supplied
17
Recommended Materials for Nucleic Acid Isolation and Quantification
18
Genexus ™ Integrated Sequencer Components
19
Genexus ™ Integrated Sequencer Deck Stations
20
Genexus Integrated Sequencer Input and Output Connections
21
Workflow
22
CHAPTER 2 Before You Begin
23
Precautions
23
Avoid Nucleic Acid Contamination
23
Avoid Chip Damage
23
Avoid Strong Electromagnetic Radiation
23
Protection by Equipment
23
Guidelines for Genexus
24
Power the Genexus ™ Integrated Sequencer on or off
25
Power on
25
Power off
26
Get Started with Genexus ™ Software
26
About the Genexus ™ Software User Interface
26
User-Access Levels
27
System Tracking
27
Request and Sign in to a New Account
27
Sign in
28
CHAPTER 3 Create and Manage
29
Assays (Manager/Administrator)
29
About Assays in Genexus ™ Software
29
System-Installed Assays
30
Manage Assays (Manager/Administrator)
30
Create a New Assay (Manager/Administrator)
32
Copy an Assay (Manager/Administrator)
40
Import an Assay (Manager/Administrator)
41
CHAPTER 4 Enter Samples and Libraries
42
Add a New Sample
42
System-Installed Sample Attributes
43
Create a Custom Sample Attribute (Manager/ Administrator)
44
Import Samples
45
Manage Samples
46
Sort, Search, and Filter Samples
46
Export and Print Samples
47
View Notes or Add a Note to a Sample
48
Edit a Sample (Manager/ Administrator)
48
Edit a Sample and Amend a Report after a Run (Manager/ Administrator)
49
Review Sample Edit History
49
Delete Samples
50
Prepare or Import a Library Batch
50
Prepare a Library Batch
50
Import a Library Batch
53
CHAPTER 5 Create and Manage Run Plans
55
Create a Run Plan for a Sample Run
56
Create a Run Plan for a Library Run
63
CHAPTER 6 Dilute the Samples and Load the Sample Plate
68
Guidelines for Nucleic Acid Isolation and Quantification-Sample Runs
68
Dilute the Samples (if Needed) and Load the Sample Plate-Sample Run
69
Guidelines for Library Quantification-Library Runs
71
Dilute and Pool Libraries, and Load the Sample Plate-Library Run
71
CHAPTER 7 Load the Sequencer and Start a Run
72
Before You Begin
72
Fill Genexus ™ Primer Pool Tubes (Custom Assays Only)
74
Load the Sequencer and Start a Run
75
Clear the Instrument Deck and Perform a UV Clean
81
CHAPTER 8 Monitor the Run
84
View Run Progress on the Instrument
84
CHAPTER 9 Review Data and Results
85
Run Results Screen
86
Lab Report
88
Download the Lab Report
89
View Sequencing Results and Run Metrics
89
Summary of the Sample Results
91
Run Metrics
92
QC Results
94
SNV/INDEL Results
95
Fusion Results
97
CNV Results
99
View Annotation Sources
101
Create and Assign Variant Classifications
102
Filter Results
102
View Oncomine ™ TCR Beta-LR Assay GX Run Results
105
Sign off on the Run Results (Manager/ Administrator)
107
Sign off on the Run Results (Manager/ a Dministrator)
107
Generate Customized Reports
108
Results Files
109
Review Coverageanalysis Plugin Results
111
Upload Sample Results Files to Ion Reporter ™ Software
111
Software
111
Verification Runs
113
View and Sign off on the Verification Run Results (Field Service Engineer)
114
APPENDIX A Troubleshooting
117
Genexus ™ Software
117
Genexus ™ Integrated Sequencer
119
APPENDIX B Touchscreen Reference
120
Touchscreen Icons
120
Settings
121
Network Settings
122
Perform a Clean Instrument Operation
124
System Tools
124
Data Management
129
Instrument Settings
131
APPENDIX C Supplemental Information
134
Maintain the Sequencer
134
Materials Required
134
Clean or Decontaminate the Sequencer
134
Replace the Genexus
135
Quantify FFPE DNA with the Qubit
135
Filter
135
Fluorometer
135
Guidelines for Using Custom Assays with the Genexus
136
Integrated Sequencer
136
Planning Sequencing Runs for Efficient Use of Consumables
138
Configure an Ion Reporter
139
Server Account (Administrator)
139
Tag an Ion Reporter
140
Ionreporteruploader Plugin
140
Configure Thermo Fisher (Connect) Accounts (Manager/Administrator)
141
APPENDIX D Coverageanalysis Plugin in Genexussoftware
143
Reads Statistics
143
Software
143
Example Coverage Analysis Report
145
Example Charts Generated by the Coverageanalysis Plugin
147
Output Files Generated by the Coverageanalysis Plugin
148
APPENDIX E Safety
150
Symbols on this Instrument
150
Standard Safety Symbols
150
Additional Safety Symbols
152
Location of Safety Labels
152
Control and Connection Symbols
153
Conformity Symbols
153
Instrument Safety
154
General
154
Physical Injury
154
Electrical Safety
155
Cleaning and Decontamination
156
Instrument Component and Accessory Disposal
156
Safety and Electromagnetic Compatibility (EMC) Standards
156
Safety Standards
157
EMC Standards
157
Environmental Design Standards
158
Radio Compliance Standards
158
Chemical Safety
159
Biological Hazard Safety
161
APPENDIX F Documentation and Support
162
Related Documentation
162
Customer and Technical Support
162
Limited Product Warranty
162
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