Gradient Delay Volume, Dwell Volume, And Ripple - Thermo Scientific Vanquish VH-P10 Operating Manual

6 • Operation
6.9.2

Gradient Delay Volume, Dwell Volume, and Ripple

Page 100
The gradient delay volume of an HPLC system is defined as the volume
of the entire flow path between the point where the solvent streams
from the pump heads are combined and the point where the mobile
phase enters the column. Thus, the gradient delay volume of an HPLC
system is the volume that the pump must deliver until a change in
solvent composition reaches the column inlet.
Figure 28: Gradient delay volume of an HPLC system
No. Description
1 Pump with
1a Point where the solvent streams from both pump heads are combined
(purge unit)
2 Autosampler
3 Column
4 Detector
5 Gradient delay volume of the system
The pump contributes to the system gradient delay volume by the dwell
volume, which is the calculated volume between the point where the
solvent streams are combined and the pump outlet. For the dwell
volume of the pump in the standard configuration, see
Performance Specifications, page 204.
Vanquish Pumps (VH-P10) Operating Manual
section 9.1