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Thermo Scientific Surveyor LC Pump Plus Hardware Manual

Thermo Scientific Surveyor LC Pump Plus Hardware Manual

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Thermo
Surveyor LC Pump Plus
Hardware Manual
60053-97120 Revision G
December 2013

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Summary of Contents for Thermo Scientific Surveyor LC Pump Plus

  • Page 1 Thermo Surveyor LC Pump Plus Hardware Manual 60053-97120 Revision G December 2013...
  • Page 2 © 2013 Thermo Fisher Scientific Inc. All rights reserved. LightPipe is a trademark and Xcalibur, Surveyor, Foundation, and ChromQuest are registered trademarks of Thermo Fisher Scientific Inc. in the United States. Super Flangeless is a trademark of IDEX Health & Science, LLC. PEEK is a trademark of Vitrex PLC. The following are registered trademarks in the United States and other countries: Windows is a registered trademark of Microsoft Corporation.
  • Page 3 Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards, replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives. Surveyor LC Pump Plus EMC Directive 89/336/EEC EMC compliance has been evaluated by Underwriters Laboratories Inc.
  • Page 4 FCC Compliance Statement THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO THE FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED, INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION. CAUTION Read and understand the various precautionary notes, signs, and symbols contained inside this manual pertaining to the safe use and operation of this product before using the device.
  • Page 5 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 6 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
  • Page 7 CAUTION Symbol CAUTION VORSICHT PRECAUCIÓN MISE EN GARDE Risk electric shock: This instrument uses Stromschlaggefahr: Dieses Gerät arbeitet mit Riesgo de descargas eléctricas: Este instrumento Risque de choc électrique : l’instrument utilise des voltages that can cause electric shock and/or Spannungen, die Stromschläge und/oder utiliza voltajes que pueden causar descargas tensions susceptibles de provoquer une électrocution...
  • Page 8 CAUTION Symbol CAUTION VORSICHT PRECAUCIÓN MISE EN GARDE Laser hazard: This instrument uses a laser that is Gefahr durch Laserstrahlen: Der in diesem Gerät Peligro por láser: Este instrumento utiliza un láser Danger lié au laser : l’instrument utilise un laser capable of causing personal injury.
  • Page 9 CAUTION Symbol CAUTION 警告 危险警告 Risk electric shock: This instrument 触电危险：本仪器所用电压可能导致电击或人身伤害。进行维修服务 感電の危険性 : この機器では、感電および / または身体傷害を引き起こ uses voltages that can cause electric 前，务必关闭仪器电源并断开其电源连接。操作此仪器时，不要卸下 すおそれのある電圧を使用しています。整備点検の前には、機器の電 shock and/or personal injury. Before 顶盖。勿卸下印刷电路板组件 （PCBA）的保护盖。 源を切り、電源コードを抜いてください。機器の作動中は、カバーを servicing, shut down the instrument and 付けたままにしてください。プリント基板アセンブリ (PCBA) から保護 disconnect it from line power.
  • Page 10 CAUTION Symbol CAUTION 警告 危险警告 レーザー光線の危険性 : この機器では、身体傷害を引き起こすおそれ 激光危险：本仪器所用激光会导致人身伤害。此符号也会在仪器上出 Laser hazard: This instrument uses a laser that is capable of causing personal のあるレーザーを使用しています。この標識記号は機器にも表示され 现。有关此危险的详细信息，参阅适当的仪器手册。 injury. This symbol also appears on the ています。この危険の詳細については、機器のマニュアルを参照して instrument. For details about the hazard, ください。 refer to the instrument manual. Ultra violet light hazard: Do not look 紫外光の危険性...

  • Page 11: Table Of Contents

    Calibrating the LC Pump ......... 29 Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 12 Checking the Pressure Pulsation of the LC Pump ..... 81 Troubleshooting the Cause of Excessive Pressure Variation ... . . 82 Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 13 Index ............. . .121 Thermo Scientific Surveyor LC Pump Plus Hardware Manual xiii...

  • Page 15: Preface

    Note Highlights information of general interest. Tip Highlights helpful information that can make a task easier. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 16: Good Laboratory Practices

    0.2 μm (or less) fluorocarbon filter before placing them in the solvent reservoir significantly prolongs the life and effectiveness of the inlet filters, check valves, piston seals, autosampler injection valve, and LC column. Typically, HPLC-grade solvents do not require filtration. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 17 LC system. However, if a leak occurs, correct it as soon as possible. Always wear eye and skin protection when operating or maintaining an LC system. Always shut down the system and return it to atmospheric pressure before attempting any maintenance. Thermo Scientific Surveyor LC Pump Plus Hardware Manual xvii...

  • Page 18: Contacting Us

    To suggest changes to the documentation or to the Help • Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62. • Send an e-mail message to the Technical Publications Editor at techpubs-lcms@thermofisher.com. xviii Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 19: Introduction

    Introduction This chapter introduces the Surveyor LC Pump Plus (see Figure 1), a member of the Surveyor Plus™ family of liquid chromatography (LC) instruments. Contents • Functional Description • Status LEDs • Flow Control • Specifications Figure 1. Surveyor LC Pump Plus...

  • Page 20: Functional Description

    Functional Description Functional Description The Surveyor LC Pump Plus is a dual-piston, quaternary, low-pressure mixing pump with a built-in vacuum degasser and pulse dampener. The pumping system provides flow rates from 0.001 to 9.999 mL/min and can run precise gradients from 0.200 to 2.000 mL/min. The integral vacuum degasser offers efficient solvent degassing while requiring only 500 μL of...

  • Page 21: Vacuum Degasser Assembly

    Introduction Functional Description Vacuum Degasser Assembly The Surveyor LC Pump Plus contains a vacuum-membrane degasser that removes dissolved gases from the mobile phase. The solvent degassing system consists of four independent chambers (see Figure 3) maintained at a constant vacuum of approximately 27 in. Hg. Each chamber contains an 18 in.
  • Page 22 Figure 4. Pump heads with tubing connections Crossover Outlet tube tube Primary Secondary pump head pump head DRAIN Purge manifold Inlet check Outlet check valve valve Solvent line from proportioning valve Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 23 Hastelloy™ C that forces the inside flange of the seal against the piston to prevent leaks. The seals are not leak proof. In fact, wetting the surface of the pistons requires a Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 24 Because check valves must have a very precise fit between the balls and seat, particulate matter compromises the sealing action of the check valves and causes erratic flow rates. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 25 Solvent path Low-pressure solvent lines Primary pump Secondary pump Solvent reservoir bottles head head Inlet check Outlet check valve valve Solvent proportioning assembly Figure 7. Check valve (schematic) Spring Ruby balls Sapphire seats Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 26 Figure 8 shows the setup for rinsing the pump head assemblies. Figure 8. Solvent flow path for the pump head assemblies Wash tubing assembly Wash seal Piston seal Rinsing tube assembly Syringe Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 27: Purge Manifold Assembly

    The replacement frequency for the inline filter frit depends on the purity of your mobile phase solvents and the wear rate for the pump piston seals. Figure 10. Line filter body and frit Line filter body Frit Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 28: Pulse Dampening Assembly

    The pulse dampening assembly is located behind the front panel of the Surveyor LC Pump Plus. This dampening coil effectively smooths the delivery of mobile phase to the rest of the HPLC, while adding only 400 μL of gradient delay volume. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 29: Status Leds

    (see Figure 12). Table 1 lists the LED states. Figure 12. Status LEDs Table 1. States and their meaning for the Surveyor LC Pump Plus LEDs Status Meaning Power Green The LC pump is switched on and receiving power.

  • Page 30: Flow Control

    Flow Control Figure 13 contains a schematic of the Surveyor LC Pump Plus flow control hardware. A stepping motor that runs at 12 800 pulses per revolution controls the flow rate of the mobile phase. The stepping motor drives a camshaft that is fitted with two cams. As the cams rotate, the cam-followers at the back of the pistons translate the rotary motion of the cams into linear motion.
  • Page 31 Introduction Flow Control Figure 13. Flow control of the Surveyor LC Pump Plus by the step motor To autosampler Drain valve diversion To waste Pressure sensor Purge manifold Pulse Outlet tube dampener Secondary Stepping pump head motor Outlet check valve...
  • Page 32 Introduction Flow Control Figure 14. Composite flow rate produced by the two reciprocating pistons Primary piston Delivery stroke [degrees] Intake stroke Secondary piston Delivery stroke Intake 180 200 stroke Composite flow rate Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 33: Specifications

    Introduction Specifications Specifications The Surveyor LC Pump Plus has the following specifications: Minimum Flow Rate: 0.001 mL/min isocratic, 0.100 mL/min gradient Maximum Flow Rate: 9.999 mL/min Flow Rate Accuracy: ±2% or ±2 μL/min, whichever is greater when water is pumped at 0.100 to 5.000 mL/min, 10 to 400 bar, 20 °C Flow Rate Precision: ±0.3% when water is pumped at 0.100 to 5 mL/min,...

  • Page 35: Installation

    Installation Follow the procedures in this chapter to install your Surveyor LC Pump Plus or to troubleshoot the Surveyor LC system connections. Use the “Installation Checklist” page 18 as a quick reference to conducting a successful installation. Make a copy of the checklist and fill it out when the installation is complete.

  • Page 36: Installation Checklist

    Installation Installation Checklist Installation Checklist The following checklist summarizes the installation steps for the Surveyor LC Pump Plus.  Unpack and inspect the instrument. (page  Read the safety notices (in the preface of this manual).  Make the initial instrument preparations.

  • Page 37: Unpacking And Inspecting The Instrument

    If you find any damage, save the shipping materials and immediately contact the shipping company. The shipping container should contain the following items: • Surveyor LC Pump Plus • Surveyor LC Pump Plus Accessory Kit (see “Surveyor LC Pump Accessory Kit” page 104) •...

  • Page 38: Connecting The Back Panel Cables

    Connecting the Back Panel Cables Connecting the Back Panel Cables Proper operation of the Surveyor LC Pump Plus from your Thermo Scientific data system requires connecting an Ethernet cable and a system synchronization cable to the back panel of the pump (see Figure 15).

  • Page 39: System Synchronization Cable

    To connect the cable with five combicon connectors to the Surveyor system 1. To connect the Surveyor LC Pump Plus, plug the connector with the blue sticker labeled LC PUMP into the eight-pin socket on the pump’s back panel (see...

  • Page 40: Ethernet Cable

    The arrow on the left switch points to the “tens” digit of the unit ID. The arrow on the right switch points to the “ones” digit of the unit ID. The Surveyor LC Pump Plus ships with the unit ID preset to 1.

  • Page 41: Low-Pressure Solvent Reservoir Lines

    These lines are used to deliver each of the four solvents to the vacuum degassing assembly inside the Surveyor LC Pump Plus. The accessory kit also includes four solvent reservoir bottle caps as well as a set of stick-on labels with the letters A, B, C, and D.
  • Page 42 17). When you place the three components of the fitting on the end of a solvent line, the flat side of the ring faces the nut and the angled side of the ring faces the ferrule. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 43 Compression tool b. Insert the tubing with the fitting assembly into the other end of the union. c. Press the tubing to the bottom of the tool while tightening down the nut. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 44 6. Repeat this procedure for the B, C, and D solvent reservoir lines. Figure 20 shows the solvent reservoir lines connected to the inlet ports of the vacuum degassing unit. Figure 20. Vacuum degas connection Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 45: High-Pressure Solvent Line Between The Pump And The Autosampler

    To connect the heat exchanger inlet tubing from the autosampler to the pump 1. Place the Surveyor Autosampler Plus on top of the Surveyor LC Pump Plus. Locate the autosampler inlet tubing that extends out of the column oven area.

  • Page 46: Powering On The Lc Pump For The First Time

    The Power LED turns solid green. If the LED does not illuminate, see the first troubleshooting tip in Table 8 page 98. If you cannot resolve the problem, call your Thermo Fisher Scientific service representative for assistance. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 47: Preparing The Lc Pump For Daily Operation

    Calibrating the LC Pump The Surveyor LC Pump Plus is factory calibrated to produce a flow rate of 1 mL/min with distilled water at 20 °C. The flow rate adjustment (alpha) value is printed on the motor casing of the pump.

  • Page 49: Software Instrument Configuration

    Software Instrument Configuration You can control the Surveyor LC Pump Plus from a Thermo Scientific™ data system. To add the LC pump to the instrument configuration and specify the signal polarities for the autosampler, follow the appropriate procedure in this chapter.

  • Page 50: Pump Configuration Options In The Chromquest Data System

    Adding the Pump to the ChromQuest Instrument Configuration Pump Configuration Options in the ChromQuest Data System The following procedure assumes that you are adding the Surveyor LC Pump Plus to a Surveyor instrument that already exists in the ChromQuest Enterprise. If you have not yet added an instrument to your Enterprise, refer to the Surveyor Plus User Guide for the ChromQuest Data System.
  • Page 51 Choose Configure > Instrument from the shortcut menu (see Figure 26). Figure 26. Shortcut menu for an instrument The Instrument Configuration dialog box appears (see Figure 27). Figure 27. Instrument Configuration dialog box Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 52 Modules area to add the pump to the instrument configuration. The Surveyor LC Pump icon moves to the Configured Modules pane (see Figure 29). Figure 29. Surveyor dialog box with added modules Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 53 Click OK to close the dialog box. 8. Do not close the Surveyor dialog box. Go to the next procedure to modify the signal polarities for the Surveyor Autosampler configuration. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 54: Autosampler Input Signals In The Chromquest Data System

    3. Exit the Configuration application and return to the Main Menu window as follows: a. Click OK to close the Surveyor AS Configuration dialog box. b. Click OK to close the Surveyor dialog box. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 55: Adding The Pump To The Thermo Foundation Instrument Configuration

    • Autosampler Input Signals in the Thermo Foundation Instrument Configuration Tip If you are controlling the Surveyor LC Pump Plus from the Xcalibur™ 2.0.7 data system or earlier, use the Instrument Configuration window to set up the instrument configuration for the Surveyor Plus devices. To open the Instrument Configuration window, choose Start >...
  • Page 56 4. In the Configured Devices area, double-click the Surveyor LC Pump icon. The Surveyor LC Pump dialog box appears (see Figure 33). Figure 33. Surveyor LC Pump configuration dialog box (Thermo Foundation platform) Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 57: Autosampler Input Signals In The Thermo Foundation Instrument Configuration

    2. In the Configured Devices area, double-click the Surveyor Autosampler icon. The Surveyor Autosampler Configuration dialog box appears with the Tray page displayed. 3. Click the Signal polarity tab. The Signal polarity page appears (see Figure 34). Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 58 4. Make sure that the Pump Ready Active High and the Injection Hold Release Active High check boxes are selected. 5. Click OK to close the Surveyor Autosampler Configuration dialog box. 6. Click Done to close the Instrument Configuration window. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 59: Maintenance

    Maintenance The five main components of the Surveyor LC Pump Plus are the degassing unit, the solvent proportioning unit, the pulse dampener, the pump head assemblies, and the purge manifold assembly. You are responsible for the routine maintenance of the pump head assemblies. You are also responsible for replacing the frit that is inside the line filter body connected to the top of the purge manifold assembly.

  • Page 60: Maintenance Schedule

    The high-pressure connections of the LC pump are made with standard 316 stainless steel tubing and two-piece fittings as shown in Figure Figure 35. Standard 316 stainless steel tubing and two-piece fittings Tube Male nut Ferrule Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 61: Maintenance Page In The Chromquest Data System

    Maintenance Page in the ChromQuest Data System Maintenance Page in the ChromQuest Data System If you are controlling the Surveyor LC Pump Plus from the ChromQuest data system, you must open the Maintenance page to perform and record maintenance on the pump head assemblies.

  • Page 62: Recording Maintenance In The Chromquest Data System

    Recording Maintenance in the ChromQuest Data System The ChromQuest data system has a built-in feature for tracking the volume of mobile phase pumped by the Surveyor LC Pump Plus. This information, which pertains to seal wear, appears in the Maintenance page.

  • Page 63: Setting The Pistons To The Maintenance Position From The Chromquest Data System

    2. Click OK to set the pistons to the maintenance position. Maintenance Page in the Xcalibur Data System If you are operating the Surveyor LC Pump Plus from the Xcalibur data system, use the Maintenance page to set the pistons to the maintenance position and record maintenance.

  • Page 64: Opening The Maintenance Page In The Xcalibur Data System

    4. From the menu bar, choose Surveyor LC Pump > Direct Control > Maintenance. The Maintenance – Surveyor LC Pump Direct Control dialog box appears (see Figure 39). Figure 39. Maintenance – Surveyor LC Pump Direct Control dialog box Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 65: Recording Maintenance In The Xcalibur Data System

    Maintenance Page in the Xcalibur Data System Recording Maintenance in the Xcalibur Data System The Surveyor LC Pump Plus view of the Instrument Setup window in the Xcalibur data system has a built-in feature for tracking the volume of mobile phase pumped by the Surveyor LC Pump Plus.

  • Page 66: Rinsing The Pistons

    • For boric acid buffers (low concentration), rinse every few hours. Rinsing between the piston seals and the wash seals requires these items: Description Part number Rinsing tube assembly 00950-30001 Disposable syringe 3301-0151 Beaker to be used as a waste-water container Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 67 5. Insert the needle of the disposable syringe into the other rinsing tube. Depress the syringe plunger to force water into the tubing and through the pump heads. Collect the wastewater in the beaker (see Figure 41). Figure 41. Rinsing behind the pump heads Waste Syringe Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 68: Cleaning The Check Valves

    If pumping IPA through the pump fails to clean the check valves, remove them from the pump, and then sonicate them in a solution of IPA as described in the next procedure. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 69: Sonicating The Check Valves

    Use a 10 mm open-end wrench to hold the outlet check valve. Then use an 8 mm open-end wrench to unscrew the fitting that connects the crossover tube to the outlet check valve. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 70 Outlet check valve (10 mm) 8 mm Step 2b 3. Unscrew the solvent line from the inlet check valve (see Figure 44). Figure 44. Removing the solvent line Inlet check valve Solvent line Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 71 To clean the check valves 1. Put the inlet and outlet check valves in an ultrasonic bath filled with IPA. Sonicate them for approximately 5 minutes. 2. Remove the check valves from the bath. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 72: Maintaining The Pump Head Assemblies

    To maintain the pump head assemblies, follow these procedures: • Replacing the Piston Seals • Replacing the Wash Seals • Cleaning and Replacing a Piston Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 73: Replacing The Piston Seals

    Installing New Piston Seals Reinstalling the Pump Heads Removing the Pump Heads The Surveyor LC Pump Plus has two pump heads. Each pump head contains a piston seal that requires periodic replacement.  To remove one or both of the pump heads 1.
  • Page 74 5. Using a 3 mm L-hex wrench, alternately loosen and then remove the two hex socket head bolts that hold the pump heads to the pump head holder as shown in Figure Figure 47. Unscrewing the hex socket head bolts Left pump head Right pump head Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 75 To install and remove the piston and wash seals, use the special double-ended tool (seal tool) in the Surveyor LC Pump Accessory Kit (see Figure 49). Figure 49. Double-ended tool for installing and removing seals (seal tool) Headless end End with head Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 76 Therefore, take care not to lose it as you pull the piston seal out of the pump head. Figure 51. Removing the piston seal Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 77 Align the groove in the pump head with the alignment pin, and then mount the pump head onto the head holder (see Figure 53). Figure 53. Aligning the pump head with the alignment pin Left pump Alignment head Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 78 10 mm open-end wrench. b. Screw the outlet tube into the top of the right pump head, and then tighten the connection with an 8 mm open-end wrench. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 79 Figure 57. Reconnecting the crossover tube Step 4a Crossover tube 8 mm Outlet check valve (10 mm) 8 mm Step 4b Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 80: Replacing The Wash Seals

    Replaceable parts Part number O-ring, wash seal holder 00950-01-00108 Wash seal 00950-01-00084 To replace the wash seals, follow these steps: Removing the Wash Seal Holders Removing a Wash Seal Installing a Wash Seal Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 81 Fit the piston removal tool over the wash seal holder, and then screw in the two socket head cap screws until they contact the washing flow line openings in the wash seal holder (see Figure 60). Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 82 Alternately tighten the bolts by using the L-hex wrench until they press against the pump head holder (see Figure 61). The compression of the two bolts on the pump head holder forces the ring holder out. Figure 61. Tightening the hex socket head bolts Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 83 3. Replace the two screws and tighten with a Phillips screwdriver. 4. Reinstall the wash seal holder by carefully sliding it over the piston. 5. Reinstall the pump heads as described in “Reinstalling the Pump Heads” page Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 84: Cleaning And Replacing A Piston

    “Removing the Wash Seal Holders” page 3. Using the hex socket end of the piston removal tool, rotate the piston nut counterclockwise, and then remove the piston assembly from the piston chamber (see Figure 63). Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 85 3. If you cannot remove caked-on deposits with IPA, use a mild abrasive, such as toothpaste. Then rinse the piston with water, followed by IPA. 4. If the piston is scratched, replace it with a new piston assembly. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 86: Replacing The Line Filter Frit

    Replace the frit if the system pressure increases beyond its normal limits. Replacing the line filter frit requires these tools and parts: Tools Part number Two 8 mm × 10 mm open-end wrenches 00950-30005 Replaceable parts Part number Filter frit 00950-30009 Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 87 Figure 67. Line filter body removed from purge manifold assembly Line filter body To remove 3. Remove the dirty frit from the line filter body (see Figure 68). Figure 68. Removing the frit Nippers Frit Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 88: Replacing The Solvent Reservoir Filter

    10 μm pore size, stainless steel filter A4929-010 Figure 69 shows the Teflon filter. Figure 69. Solvent reservoir filter and solvent line End of solvent line (that has been scored by the solvent reservoir filter) Solvent reservoir filter Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 89: Purging Air From The Solvent Lines Or The Pump Heads

    You must open the drain valve before you use the purge feature.  To open the drain valve Turn the drain valve knob 180° counterclockwise as shown in Figure Figure 70. Drain valve in the purge position Open position Closed position DRAIN Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 90: Using The Purge Feature

    Click Diagnostics. The Diagnostics dialog box appears. d. Click the Operation tab. The Operation page appears (see Figure 71). Figure 71. Operation page of the Diagnostics dialog box (ChromQuest data system) Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 91 In the Solvent Valve list, select the solvent line that you want to purge: A, B, C, or D. The Surveyor LC Pump Plus is a quaternary pump capable of proportioning up to four solvents. If the solvent lines have been plumbed correctly, the solvent valves should reflect the contents of the solvent lines and the respective solvent bottles in the solvent platform at the top of the Surveyor stack.
  • Page 92 Click Purge. The pump flow increases to approximately 10 mL/min. 4. After you finish purging the line, close the purge valve by gently turning the drain valve knob clockwise until you feel resistance. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 93: Replacing The Drain Valve Knob O-Ring

    2. Using needle-nose pliers, remove the O-ring from the drain valve shaft. 3. Place a new O-ring on the shaft. 4. Screw the drain valve knob back into the purge manifold assembly. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 94: Replacing The Fuses

    Figure 74. Using a screwdriver to pry off the fuse holder 3. Pull out the fuse holder, and remove the fuses (see Figure 75). Figure 75. Fuses in fuse holder Fuses Fuse holder Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 95 Figure 76. Placing a new fuse into the fuse holder Fuse 5. Push the fuse holder into its socket until it clicks in place. 6. Plug the power cord into line power and turn on the power to the pump. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 97: Diagnostic Tests And Calibration Options

    2.9 to 9.8 MPa (420 to 1420 psi) at a flow rate of 1.0 mL/min • 10 mL graduated cylinder to measure volume • Analytical balance (optional) to indirectly measure volume • Stopwatch to measure elapsed time Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 98 (g)] / density of water = Volume (mL) filled Where: = weight of the graduated cylinder filled with water filled = weight of the dry graduated cylinder Volume = volume of water in the graduated cylinder Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 99: Pressure Pulsation Check

    Purge the flow line that connects the solvent reservoir to the solvent proportioning valve until water replaces the former solvent in the flow line. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 100: Troubleshooting The Cause Of Excessive Pressure Variation

    “Cleaning and Replacing a Piston” page 66. If you detect a leaking wash seal, replace it as described in “Replacing the Wash Seals” page Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 101: Proportioning Accuracy Check

    Proportioning Accuracy Test—Conditions • Proportioning Accuracy Test—Running Proportioning Accuracy Test—Preparation To perform the proportioning accuracy test, prepare a spiked methanol solution and set up your instrument. Material Part number HPLC-grade methanol Ethyl paraben A4904-010 Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 102: Proportioning Accuracy Test-Conditions

    Table 5 lists the gradient program for testing proportioning accuracy. The gradient profile produced by this program contains four steps (see Figure 77 page 86). Inaccuracies in the step heights indicate proportioning errors. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 103: Proportioning Accuracy Test-Running

    10.0 40.01 100.0 50.00 100.0 Proportioning Accuracy Test—Running To test the proportioning accuracy of your Surveyor LC Pump Plus, prepare your instrument as described in “Proportioning Accuracy Test—Preparation” page 83, create an instrument method as described in “Proportioning Accuracy Test—Conditions”...

  • Page 104: Analyzing The Test Results

    Figure 77. Record the absorbance levels at the set concentrations of 0%, 10%, 50%, 90%, and 100% spiked eluent. Figure 77. Step gradient for testing the solvent proportioning of your LC pump Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 105 If the pump fails the proportioning test, try to restore proper performance by running the pump for one hour with 100% IPA on all four channels. If cleaning the proportioning valves fails to restore proper pump performance, contact your local Thermo Fisher Scientific service representative. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 106: Gradient Delay Volume Check

    Subtract the time required to switch mobile phases from 100% A to 100% B. For the pump program given, this switch time is 0.01 minutes. c. Determine the gradient delay volume by multiplying the resulting gradient delay time by the flow rate. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 107 Figure 78. Graphical display of the baseline shift that occurs as the spiked mobile phase reaches the detector Pump to detector Injector to Pump to detector injector Baseline shift Unretained peak Inject Time (in minutes) Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 108: Calibration Options

    • Pressure Transducer Zero Tip For information about accessing these calibration options, refer to the data system Help for the Surveyor LC Pump Plus or the Surveyor Plus user guide for your Thermo Scientific data system. Compressibility The compressibility of a liquid is a measure of its resistance to a decrease in volume caused by an increase in pressure.
  • Page 109 Table 6. Measured flow rate and pressure pulsation versus compressibility setting Compressibility setting % Pressure pulsation Actual flow rate (mL/min) % Rel diff –1 0.45 GPa ±0.2% 3.01 +0.3% –1 1.25 GPa ±0.4% 3.05 +1.8% –1 3.0 GPa ±1% 3.18 +5.9% Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 110: Pressure Recorder Full Scale

    = 49 bar = 711 psi Tip Record the scaling factor. This parameter is not backed up when the power is turned off. Figure 80. Terminals located on the back panel of the Surveyor LC Pump Plus UNIT ID ENET...

  • Page 111: Flow Rate Adjustment (Alpha)

    Calibration Options Flow Rate Adjustment (Alpha) The alpha value for your Surveyor LC Pump Plus is factory-calibrated based on a flow rate of 1.000 mL/min for distilled water. A sticker containing the factory-calibration value is located inside the pump on the motor casing. You can optimize the alpha value for your application by updating the alpha setting.

  • Page 113: Troubleshooting

    Troubleshooting If properly maintained, the Surveyor LC Pump Plus will operate trouble-free for many years. You can avoid most pump problems by simple, periodic maintenance, as described in Chapter 4, “Maintenance.” However, in the event that an error message is displayed or if you suspect a mechanical or electrical failure, use the troubleshooting information in this chapter to diagnose the problem.

  • Page 114: Troubleshooting Tips

    1. System column not equilibrated; allow more equilibration time. 2. Refer to the troubleshooting guide in the hardware manual for your detector.  Baseline Noise 1. Check for air bubbles in the system. 2. Check for solvent contamination. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 115 1. Check cable connections. 2. Check the system software configuration. 3. Refer to the troubleshooting guide for the instrument in your LC system that is not communicating with the chromatography data system. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 116: Lc Pump Troubleshooting Guide

    LC Pump Troubleshooting Guide LC Pump Troubleshooting Guide Table 8 lists common pump-related problems and suggestions for corrective action. Table 8. Surveyor LC Pump Plus Troubleshooting Guide (Sheet 1 of 2) Symptom or malfunction Possible cause Corrective action 1. The power does not come The power plug is disconnected.
  • Page 117 Troubleshooting LC Pump Troubleshooting Guide Table 8. Surveyor LC Pump Plus Troubleshooting Guide, continued (Sheet 2 of 2) Symptom or malfunction Possible cause Corrective action 5. The flow rate is below the The check valve is not working properly. Pump IPA through the flow line to clean set value.

  • Page 118: Pump Pulsation

    Abnormal Pressure Pulsation Normal Pressure Pulsation Under normal operating conditions, the Surveyor LC Pump Plus shows very small variation in pressure. The pressure variation is about 4 bar with a backpressure of 200 bar (1 bar = 0.10 MPa = 14.5 psi).

  • Page 119: Baseline Noise At Low Pressures (Under 40 Bar)

    You can usually fix this problem by adding a flow restrictor to your system between the Surveyor LC Pump Plus and the autosampler to increase backpressure back to a level greater than 40 bar. Capillary LC at 3 to 5 μL/min and 15 to 20 bar (for example, a 300 μm column at 3 μL/min) is a typical application susceptible to this problem.

  • Page 120: Blocked Proportioning Valve

    “Proportioning Accuracy Test—Running” page Inaccuracy of the steps is most likely caused by contamination of the proportioning valves. Run the pump at least 2 hours with isopropyl alcohol before repeating the test. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 121: Accessories And Replaceable Parts

    Accessories and Replaceable Parts This chapter contains a list of parts and tools for maintaining the Surveyor LC Pump Plus, and a list of parts that only a Thermo Fisher Scientific field service engineer can replace. To order parts, contact your local Thermo Fisher Scientific service representative. To facilitate the ordering process, part numbers are listed next to their respective parts.

  • Page 122: Surveyor Lc Pump Accessory Kit

    Kit, Surveyor LC Pump Plus Accessory ......60053-62003 Assembly, drain tube, OEM LC pump .

  • Page 123: Replacement Parts Required For Routine Maintenance

    Wash tube assembly..........00950-30019 Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 124: Nonconsumable Replacement Parts

    Chassis, Surveyor LC Pump Plus........60053-10001...

  • Page 125: Replacement Parts Required For Nonroutine Maintenance

    Valve assembly, gradient, 4-way Teflon block......00950-30003 Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 126: Solvent Interconnect Kit

    The Solvent Interconnect Kit used to connect the solvent reservoir bottles to the degas unit of the Surveyor LC Pump Plus contains these parts: Kit, Solvent Interconnect ......... F5050-010 Bottle cap, solvent bottles .

  • Page 127: Appendix A Firmware

    Firmware Periodically, upgrades to the Ethernet or CPU firmware for the Surveyor LC Pump Plus might become available. In anticipation of future upgrades, both the ChromQuest and LC Devices CD contain the Surveyor Firmware Upgrade utility and the ROM Upgrade utility.

  • Page 128: Copying The Firmware Files From The Chromquest Cd

    The Firmware folder contains folders for all the Surveyor instruments and the Surveyor Firmware Upgrade Utility. The firmware files for the ChromQuest 5.0 data system might differ from those shown in Figure Figure 81. CD drive:\ChromQuest\Firmware folder for ChromQuest 4.2 Surveyor LC Pump Plus Hardware Manual Thermo Scientific...

  • Page 129: Updating The Ethernet Firmware For The Lc Pump

    To prepare the Surveyor LC Pump Plus to download the Ethernet firmware 1. Ensure that you have the Surveyor LC Pump Plus firmware files that you intend to download and the Surveyor Firmware Upgrade Utility on the data system computer.

  • Page 130: Downloading The Communication Firmware Files

    Updating the Ethernet Firmware for the LC Pump Downloading the Communication Firmware Files  To download the communication firmware files to the Surveyor LC Pump Plus 1. Start the Surveyor Firmware Upgrade Utility by double-clicking the Surveyor Firmware Upgrade Utility application.
  • Page 131 Figure 83. Surveyor Firmware Upgrade Utility with the LC pump firmware files selected 4. After you select the appropriate firmware files, click Download, and then wait for the download to finish. The utility notifies you when the download is complete. Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

  • Page 132: Returning The Lc Pump To Normal Operation

    Firmware Updating the CPU Firmware for the LC Pump Returning the LC Pump to Normal Operation After you finish upgrading the firmware, return the Surveyor LC Pump Plus to normal operation.  To return the LC pump to normal operation 1.

  • Page 133: Preparing The Lc Pump For A Cpu Firmware Upgrade

    Preparing the LC Pump for a CPU Firmware Upgrade To prepare the Surveyor LC Pump Plus for a firmware upgrade, you must change the rotary switch position on the back panel of the pump and connect the LC pump to the data system computer with an RS232 serial communication cable.
  • Page 134 6. Using a serial communication cable, connect the computer COM port to the RS232 connection on the back panel of the LC pump (see Figure 86). Figure 86. Back panel connections between the data system computer and the Surveyor LC Pump Plus Ethernet Switch Serial Port with DB9 to RJ45 Adapter...

  • Page 135: Using The Flash Rom Update Utility To Update The Cpu Firmware

    Using the Flash ROM Update Utility to Update the CPU Firmware The file name of the CPU firmware update utility for the Surveyor LC Pump Plus is LCPUMP tf UPdate.exe. The file name of the CPU firmware for the Surveyor LC Pump Plus is LCPUMP tfv302.ROM, where 302 is the version number.
  • Page 136 The ROM Open file dialog box appears (see Figure 90). Figure 90. ROM Open file dialog box b. Browse to the following folder: drive:\Thermo\Instruments\LC Devices\Surveyor Firmware c. Select LCPUMP tfv302.ROM. d. Click Open. Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 137 9. Reset the rotary switches on the back panel of the Surveyor LC Pump Plus, disconnect the RS232 cable, and remove the 8-pin, Mini-Combicon connector with the jumper. 10. Turn on the power to the Surveyor LC Pump Plus and check the status of the LEDs. Thermo Scientific...

  • Page 139: Index

    (table) causing piston abrasion configuration, device driver rinsing from pump after use in the ChromQuest data system bushings, rinsing tube in the Foundation Instrument Configuration application Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 140 Ethernet firmware downloads solvent reservoir lines cable connections system synchronization cable rotary switch settings unpacking ethyl paraben solution, preparation instruments, configuring in ChromQuest data system isopropyl alcohol (IPA) pistons, cleaning pump heads, cleaning Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 141 Surveyor LC Pump Accessory Kit pulse dampener, description solvent interconnect kit peak area, reproducibility Thermo Scientific Surveyor LC Pump Plus Hardware Manual...
  • Page 142 75–76 seals for replacing a piston piston for replacing a piston seal cause of degradation for replacing a wash seal description trapped air, removing replacing wash description replacing sinker frit Surveyor LC Pump Plus Hardware Manual Thermo Scientific...
  • Page 143 WEEE compliance Xcalibur data system Maintenance page Maintenance Position button purge controls, LC pump Thermo Scientific Surveyor LC Pump Plus Hardware Manual...

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