Thermo Scientific Dionex AXP Operator's Manual
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Thermo Scientific Dionex AXP Operator's Manual

Thermo Scientific Dionex AXP Operator's Manual

Metering pump
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Dionex
AXP/AXP-MS Metering Pump
Operator's Manual
Document No. 031897
Revision 05
December 2011

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Summary of Contents for Thermo Scientific Dionex AXP

  • Page 1 Dionex AXP/AXP-MS Metering Pump Operator's Manual Document No. 031897 Revision 05 December 2011...
  • Page 2 For Research Use Only. Not for use in diagnostic procedures. SAFETY REGULATIONS The CE Mark label on the Dionex AXP/AXP-MS indicates that the pump is in compliance with the following standards: EN 61010-1:1993 (safety), CAN/CSA-C22.2 No. 1010.1-92 + A2:97 (safety), UL 6101A-1...

  • Page 3: Table Of Contents

    Seals ______________________________________________________________________27 General ____________________________________________________________________27 Interfacing Information__________________________________________________ 28 Rear Panel Serial Communications Port ________________________________________28 Rear Panel 6-Pin and 10-Pin Terminal Board Connectors __________________________31 Dionex AXP-MS Valve Plumbing and Port Assignments __________________________36 EZ Grip Fitting Guide _______________________________________________________37 Warranty___________________________________________________________________ 38 Customer Support ___________________________________________________________ 39...

  • Page 4: Introduction

    The Dionex AXP pump flow rate can be set from 0.01 to 10.00 mL/min (in increments of 0.01 mL) with a precision of 0.5%. The Dionex AXP-MS pump flow rate can be set from 0.01 to 1.00 mL/min (in increments of 0.01 mL) with a precision of 0.5%.
  • Page 5 Note: When the Dionex AXP is used as a sample loading pump for trace ion applications, the convoluted diaphragm will cause sample contamination.

  • Page 6: Specifications For The Dionex Axp/Axp-Ms Pump

    Dionex AXP/AXP-MS Manual 1.2 Specifications for the Dionex AXP/AXP-MS Pump Flow Rates 0.01 to 10.00 mL/min (Dionex AXP) 0.01 to 1.00 mL/min (Dionex AXP-MS) Pressure 0 to 2500 psi Flow Accuracy Flow Precision 0.5% RSD Dimensions 6.25 in. high x 7.75 in. wide x 14 in. deep...

  • Page 7: Installation

    Dionex AXP/AXP-MS Manual 2 INSTALLATION 2.1 Unpacking and Inspection Prior to opening the Dionex AXP/AXP-MS pump shipping container, inspect it for damage or evidence of mishandling. If it has been damaged or mishandled, notify the carrier before opening the container. Once the container is opened, inspect the contents for damage.
  • Page 8 Dionex AXP/AXP-MS Manual technique for degassing is to sparge the solvent with standard laboratory-grade (99.9+%) helium. Helium is only sparingly soluble in HPLC solvents, so other gases dissolved in the solvent diffuse into the helium bubbles and are swept from the system. Solvent filtration is not an effective alternative to helium degassing.
  • Page 9 2.4.4 Solvents with Harmful Effects Except for PEEK pump heads, all portions of the Dionex AXP/AXP- MS pump that contact mobile phase are manufactured of type 316 stainless steel, ceramic, sapphire, or ruby. Some of these materials are extremely sensitive to acids (including some Lewis acids) and acid halides.

  • Page 10: Instrument Installation

    PEEK tubing may be cut with a plastic tubing cutter or razor knife. If the Dionex AXP is being used as a sample loading pump for trace ion applications, the convoluted diaphragm will cause sample contamination.

  • Page 11: Preparation For Storage Or Shipping

    Dionex AXP/AXP-MS Manual 2.5.5 Priming the Pump and the Flushing Lines Connect a syringe to the priming adapter. Run the pump at a flow rate of 3.00 to 5.00 mL/min. Prime the pump by pulling mobile phase and any air bubbles through the system and into the syringe (a minimum of 20 mL).
  • Page 12 2.6.2 Packaging for Shipping CAUTION: Reship the AXP/Dionex AXP-MS in the original carton, if possible. If the original carton is not available, wrap the pump in several layers of bubble wrap and cushion the bottom, top, and all four sides with 2 in. of packaging foam. Although heavy, an HPLC pump is a delicate instrument and must be carefully packaged to withstand the shocks and vibration of shipment.

  • Page 13: Operation

    Dionex AXP/AXP-MS Manual 3 OPERATION 3.1 Front Panel Controls and Indicators ON / OFF Figure 3-1. AXP/Dionex AXP-MS Pump Front Panel 3.1.1 Control Panel 3.1.1.1 Digital Display The three-digit display shows the pump flow rate (mL/min), the system pressure (psi), or the selected upper or lower pressure limit (psi) when operating.
  • Page 14 Dionex AXP/AXP-MS Manual 3.1.1.2 Keypad When pressed, this button alternately starts and RUN/ stops the pump. STOP When pressed, this button increases the flow rate.  When pressed, this button decreases the flow rate.  When the PRIME button is pressed, the pump runs at the maximum flow rate for the pump head.
  • Page 15 Dionex AXP/AXP-MS Manual 3.1.1.4 Power-up Configuration Pressure Compensation: On power-up, press the PRIME button on the front panel while pressing the Power On switch on the rear of the pump. The pump will display a number from 0 to 60 that represents the running pressure of the pump from 0 to 6000 psi.

  • Page 16: Rear Panel Remote Input

    Ship Kit. When connecting to a USB port, the driver provided with the serial-to-USB adapter must be installed on the PC before the converter cable is attached to the USB port. See Section 7 for details on connection and operation. Figure 3-2. Dionex AXP/AXP-MS Pump Rear Panel...

  • Page 17: Theory Of Operation

    4.1 Mechanical Operation 4.1.1 Liquid System Flow Path The flow path of the Dionex AXP/AXP-MS pump starts at the inlet reservoir filter, passes through the inlet check valve, then through the pump head, and finally exits through the outlet check valve.

  • Page 18: Electronic Control

    The flow rate of any high pressure pump can vary, depending on the operating pressure and the compressibility of the fluid being pumped. The Dionex AXP/AXP-MS pump is calibrated at 1000 psi, using an 80:20 mixture of water and isopropanol.
  • Page 19 Dionex AXP/AXP-MS Manual The motor stall detector is enabled or disabled during power-up by pressing and holding the RUN/STOP and the PRIME buttons while the power is switched on. Release the buttons when the display reads SFE. To enable the motor stall detector, press the up arrow button and the display will read ON.

  • Page 20: Maintenance

    Dionex AXP/AXP-MS Manual 5 MAINTENANCE Cleaning and minor repairs of the Dionex AXP/AXP-MS pump can be performed as outlined below. 5.1 Filter Replacement 5.1.1 Inlet Filters Inlet filters should be checked periodically to ensure that they are clean and do not restrict flow. A restriction could cause cavitation and flow loss in the pump.
  • Page 21 Dionex AXP/AXP-MS Manual CAUTION: Be careful not to break the piston when removing the pump head. Twisting the pump head can cause the piston to break. 7. Carefully separate the pump head from the pump. Move the pump head straight out from the pump and remove it from the piston. Be careful not to break or damage the piston.
  • Page 22 Dionex AXP/AXP-MS Manual Figure 5-1. Self-Flushing Pump Head Assembly 5.2.3 Cleaning the Pump Head Assembly Note: If you choose to remove the piston seal or self-flush diaphragm, you should have a new set on hand to install after cleaning. It is not recommended that you reinstall the used piston seal or diaphragm, since they may be scratched and damaged during removal and would not provide a reliable seal if reused.
  • Page 23 Dionex AXP/AXP-MS Manual 2. The pump head, check valves, and flushing housing may be further cleaned using a laboratory-grade detergent solution in an ultrasonic bath for at least 30 minutes, followed by rinsing for at least 10 minutes in distilled water. Be sure that all particles loosened by the above procedures have been removed from the components before reassembly.
  • Page 24 Dionex AXP/AXP-MS Manual Figure 5-2. Check Valves 5.2.3.1 Removing the Seals 1. Remove the pump head as described in Section 5.2.1. 2. Insert the flanged end of the seal insertion/removal tool into the seal cavity on the pump head. Tilt it slightly, so that the flange is under the seal, and pull out the seal.
  • Page 25 Dionex AXP/AXP-MS Manual 5.2.3.2 Cleaning the Piston It is not necessary to remove the piston from the housing to clean the piston. Use the scouring pad included in the seal replacement kit to clean the piston. Gently squeeze the piston within a folded section of the pad and rub the pad along the length of the piston.
  • Page 26 Dionex AXP/AXP-MS Manual 5. Remove the snap ring from the groove on the old piston and place it into the groove on the new piston. 6. Place a small amount of high quality grease on the back end of the metal base of the piston assembly.

  • Page 27: Check Valve Cleaning And Replacement

    5.5 Fuse Replacement Three fuses protect the Dionex AXP/AXP-MS pump. Two of the fuses are located in the power entry module at the rear of the cabinet and are in series with the AC input line.

  • Page 28: Battery Replacement

    Dionex AXP/AXP-MS Manual 5.6 Battery Replacement The battery provides power for the memory that holds the current pump configuration. If the pump is set at a flow rate other than 1.00 or 10.00 mL/min and the power is turned off, the flow rate last selected should still be in effect when the power is turned back on.
  • Page 29 Dionex AXP/AXP-MS Manual Quick Guide to Problem Solving You Notice This May Mean Possible Cause You Should 1. Uneven pressure 1. Bubble in 1. Solvent not properly 1. Check to be certain that mobile phase is trace. check valve. degassed.

  • Page 30: List Of Replacement Parts

    Dionex AXP/AXP-MS Manual 6 LIST OF REPLACEMENT PARTS PEEK Head Part No. Description 061035 PEEK/Bioclean check valve kit 052306 0.010-in. ID x 1/16-in. OD PEEK tubing, 5 ft 052309 0.020-in. ID x 1/16-in. OD PEEK tubing, 5 ft 061038 PEEK/Bioclean coupling 061039 E-Z Grip 1/16-in.

  • Page 31: Interfacing Information

    Dionex AXP/AXP-MS Manual 7 INTERFACING INFORMATION 7.1 Rear Panel Serial Communications Port An RS-232C modular jack is provided on the rear panel. To control the pump using Chromeleon version 6.80 (or later), connect the pump to the serial or USB port on the Chromeleon PC, using the cables included in the Ship Kit.
  • Page 32 Dionex AXP/AXP-MS Manual 7.1.2 Handshaking The pump uses hardware handshaking. The pump will not transmit on the TXD output if the DSR input is at a low logic level. And, the pump will not receive on the RXD input when the DTR output is at a low logic level.
  • Page 33 Dionex AXP/AXP-MS Manual OK,xxx.x,y,z,PSI,w,v,u/ Reads the current pump setup, where: x.x, xx.x, or xxx.x = Flow rate in mL/min (x.x, xx.x, or xxx.x) y, yy, or yyy = Upper pressure limit (y, yy, or yyy) z, zz, or zzz = Lower pressure limit...

  • Page 34: Rear Panel 6-Pin And 10-Pin Terminal Board Connectors

    Dionex AXP/AXP-MS Manual OK,a.aa,b,c,d,e,f,g,h,i,j,k,l, Reads the current pump setup, where: m,n,o,p,q/ a.a, aa.a, or aaa.a = Flow rate in mL/min b = Run status (0 = stopped, 1 = running) (a.a, aa.a, or aaa.a) c or cc = Pressure compensation...
  • Page 35 Dionex AXP/AXP-MS Manual EVENT 1 (Pole), EVENT 2 (NC), and EVENT 3 (NO) terminals. When the pump stops because the sensed pressure exceeds the set pressure limits or due to a motor stall fault, the connection between the EVENT 1 terminal and the EVENT 2 and EVENT 3 terminals is affected.
  • Page 36 Dionex AXP/AXP-MS Manual 7.2.2 General Information on Inputs The pump's inputs are on the 10-pin terminal board connector. The pinout is: Function VOLTAGE COM VOLTAGE IN FREQ IN ENABLE IN PUMP-RUN PUMP-STOP No connection No connection No connection 7.2.3 General Information on Run, Stop, and Enable Inputs The PUMP-RUN, PUMP-STOP, and ENABLE IN inputs operate from an internal 5 VDC source.
  • Page 37 Dionex AXP/AXP-MS Manual Single-Signal Level: To enable this m ode of operation, the PUMP- STOP input m ust be perm anently connected to COM with a jum per wire. To start the pum p, put a low logic level on the PUMP-RUN input.
  • Page 38 Dionex AXP/AXP-MS Manual 7.2.7 Voltage Input The remote voltage flow control is implemented by connecting a negative input to the rear panel VOLTAGE COM connection and a positive input to the VOLTAGE IN connection. A 0-10 VDC input corresponds to a 0 to 10.00 mL/min flow rate. Any device capable of sourcing at least 0.0005 amp will work.

  • Page 39: Dionex Axp-Ms Valve Plumbing And Port Assignments

    Dionex AXP/AXP-MS Manual 7.3 Dionex AXP-MS Valve Plumbing and Port Assignments Figure 7-1. Liquid Connections for the Dionex AXP-MS...

  • Page 40: Ez Grip Fitting Guide

    Dionex AXP/AXP-MS Manual 7.4 EZ Grip Fitting Guide...

  • Page 41: Warranty

    Dionex AXP/AXP-MS Manual WARRANTY The product described in this manual, other than seals, check valves, inlet/outlet filters, pistons, and pulse damper, is warranted against defective material and workmanship for a period of three (3) years from the date of shipment. Seals and valves, whether sold independently or as component parts of other products, are warranted against defective material and workmanship for a period of ninety (90) days from date of shipment.

  • Page 42: Customer Support

    Dionex AXP/AXP-MS Manual CUSTOMER SUPPORT Thermo Scientific Dionex products are supported by Thermo Fisher Scientific Technical Support Representatives. For assistance, contact Technical Support for Dionex products. In the U.S. and Canada, call 1- 800-346-6390. Outside the U.S. and Canada, call the nearest Thermo...

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