Thermo Scientific Vanquish VH-P10 Operating Manual
  1. Manuals
  2. Brands
  3. Thermo Scientific Manuals
  4. Water Pump
  5. Vanquish VH-P10
  6. Operating manual

Thermo Scientific Vanquish VH-P10 Operating Manual

Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
 
 
 
thermoscientific
Vanquish
Pumps
VH-P10
Operating Manual 
4820.4401‐EN Revision 2.0a 
 
 
• 
September 2017 
 

Advertisement

Table of Contents
loading
Need help?

Need help?

Do you have a question about the Vanquish VH-P10 and is the answer not in the manual?

Questions and answers

Subscribe to Our Youtube Channel

Related Manuals for Thermo Scientific Vanquish VH-P10

Summary of Contents for Thermo Scientific Vanquish VH-P10

  • Page 1         thermoscientific Vanquish Pumps VH-P10 Operating Manual  4820.4401‐EN Revision 2.0a  •  September 2017     ...
  • Page 2 Copyright © 2015 Thermo Fisher Scientific Inc. All rights reserved. Trademarks MP35N is a registered trademark of SPS Technologies. PharMed is a registered trademark of Saint-Gobain Performance Plastics. Torx is a registered trademark of TEXTRON INDUSTRIES, INC. Acrobat, Adobe, and Adobe Reader are trademarks or registered trademarks of Adobe Systems Incorporated in the United States and other countries.
  • Page 3 Contacting Us Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact your local Thermo Fisher Scientific sales organization. For contact information, go to Contact Us on http://www.thermoscientific.com. Technical Assistance For technical support for HPLC products, contact your local Thermo Fisher Scientific support organization.
  • Page 4 Contacting Us Page 4 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 5: Table Of Contents

    Contents Contents 1 Using this Manual ................11 About this Manual ....................12 Conventions ......................13 1.2.1 Safety Messages ..................13 1.2.2 Special Notices and Informational Notes ..........13 1.2.3 Typographical Conventions ..............14 Reference Documentation ..................15 2 Safety ....................17 Safety Symbols and Signal Words .................
  • Page 6 Contents 4 Unpacking and Transport ..............41 Unpacking and Moving ..................42 Scope of Delivery ....................45 5 Installation ..................47 Safety Guidelines for Installation ................. 48 Installing the Pump ....................49 Site Requirements ....................51 Accessing the Interior Components ..............53 Setting Up the Hardware ..................
  • Page 7 Contents Optimizing the Performance of the Pump ............99 6.9.1 General Guidelines ................... 99 6.9.2 Gradient Delay Volume, Dwell Volume, and Ripple ......100 6.9.3 Installing Solvent Shut Off Valves ............106 6.10 Shutting Down the Pump ..................109 6.10.1 Short-Term Shutdown (Interruption of Operation) ....... 109 6.10.2 Long-Term Shutdown ................
  • Page 8 Contents Inline Filter ......................159 7.8.1 Testing the Inline Filter for Permeability ..........159 7.8.2 Replacing the Inline Filter or Capillary Mixer ........160 Mixing System ....................162 7.9.1 Testing the Static Mixer for Permeability ..........162 7.9.2 Replacing the Static Mixer or Capillary Mixer ........163 7.10 Solvent Lines and Solvent Line Filters ..............
  • Page 9 Contents 10.3 Optional Accessories ................... 210 10.4 Consumables and Replacement Parts ..............211 11 Appendix ..................217 11.1 Compliance Information ..................218 11.1.1 Declarations of Conformity ..............218 11.1.2 WEEE Compliance .................. 218 11.1.3 FCC Compliance ..................219 11.2 Digital I/O ......................220 12 Index ...................
  • Page 10 Contents Page 10 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 11: Using This Manual

    • Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Vanquish Pumps (VH-P10) Operating Manual Page 11...

  • Page 12: About This Manual

    • Using this Manual About this Manual This manual describes the functional features and operating principle of your Vanquish™ pump and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. The layout of this manual is designed to provide quick reference to the sections of interest to the user.

  • Page 13: Conventions

    • Using this Manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Safety Messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.

  • Page 14: Typographical Conventions

    • Using this Manual 1.2.3 Typographical Conventions These typographical conventions apply to the descriptions in this manual: Data Input and Output • The following appears in bold type: ♦ Input that you enter by the keyboard or that you select with the mouse ♦...

  • Page 15: Reference Documentation

    • Using this Manual Reference Documentation In addition to this operating manual, other documentation is available for reference. Hardware Documentation Additional hardware documentation includes the following: • Operating manuals for the other modules of the Vanquish system A printed version of the manual is shipped with the device. •...
  • Page 16 • Using this Manual Chromeleon™ 6.8 Help • The Chromeleon 6.8 Help provides extensive information for all aspects of the software, including device installation and configuration. TIP The Chromeleon Help and documents are included in the software shipment. Third-Party Documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs).

  • Page 17: Safety

    • Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the pump. Vanquish Pumps (VH-P10) Operating Manual Page 17...

  • Page 18: Safety Symbols And Signal Words

    • Safety Safety Symbols and Signal Words 2.1.1 Safety Symbols and Signal Words in This Manual This manual contains safety messages to prevent injury of the persons using the pump. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.

  • Page 19: Observing This Manual

    • Safety 2.1.2 Observing this Manual Observe the following: • Before installing or operating the pump, read this manual carefully to be familiar with the pump and this manual. The manual contains important information with regard to user safety as well as use and care of the pump.

  • Page 20: Rating Plate

    • Safety 2.1.4 Rating Plate The rating plate is present on the pump near the electrical connections. The rating plate indicates the serial number, part number, module name, line and fuse rating, and the manufacturer's address. TIP An additional type label on the leak tray of the pump indicates the serial number, part number, and module name.

  • Page 21: Intended Use

    • Safety Intended Use The pump is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze a mixture of compounds in sample solutions. The pump is for use by qualified personnel and in laboratory environment only. The pump and Vanquish system are intended for laboratory research use only.

  • Page 22: Safety Precautions

    • Safety Safety Precautions 2.3.1 General Safety Information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the pump. If the pump is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the pump could be impaired.

  • Page 23: Qualification Of The Personnel

    • Safety 2.3.2 Qualification of the Personnel Observe the information below on the proper qualification of the personnel installing and/or operating the pump. Installation Only skilled personnel are permitted to install the pump and to establish the electrical connections according to the appropriate regulations. Thermo Fisher Scientific recommends always having service personnel certified by Thermo Fisher Scientific perform the installation (for brevity, referred to as Thermo Fisher Scientific service engineer).

  • Page 24: Electrical Safety Precautions

    • Safety Protective Eyewear To prevent liquids from striking your eyes, put on appropriate protective eyewear, such as safety glasses with side shields. If there is a risk of splashing liquids, put on goggles. Gloves To protect you from harmful liquids and avoid personal injury during maintenance or service, put on appropriate protective gloves.

  • Page 25: General Residual Hazards

    • Safety 2.3.5 General Residual Hazards Pay attention to the following general residual hazards when working with the pump: WARNING—Hazardous Substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. •...
  • Page 26 • Safety WARNING—Self-Ignition of Solvents Solvents with a self-ignition temperature below 150 °C might ignite when in contact with a hot surface (for example, due to leakage in the chromatography system). Avoid the use of these solvents. WARNING—Hazardous Vapors Mobile phases and samples might contain volatile or flammable solvents.

  • Page 27: In Case Of Emergency

    • Safety 2.3.6 In Case of Emergency WARNING—Safety Hazard In case of emergency, disconnect the pump from the power line. Vanquish Pumps (VH-P10) Operating Manual Page 27...

  • Page 28: Solvent And Additive Information

    • Safety Solvent and Additive Information To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • The system must be used with reversed-phase (RP) compatible solvents and additives only. • Use only solvents and additives that are compatible with all parts in the flow path.
  • Page 29 • Safety NOTICE If the system flow path includes a Vanquish charged aerosol detector, observe the specific solvent and additive information for this detector. Refer to the operating manual for the Vanquish charged aerosol detector. • Follow any specific recommendations presented in other sections of this manual.

  • Page 30: Compliance Information

    • Safety Compliance Information Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the device is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual.

  • Page 31: Pump Overview

    • Pump Overview 3 Pump Overview This chapter introduces you to the pump and the main components. Vanquish Pumps (VH-P10) Operating Manual Page 31...

  • Page 32: Pump Features

    • Pump Overview Pump Features The pump includes the following main features: • Two-channel pump with each channel based on a dual-piston in- parallel design • Independent drive for each piston for high accuracy and increased flexibility • Active piston seal wash system for high lifetime of pistons and piston seals The piston seal wash system keeps the pistons wet and rinses the piston seals automatically.

  • Page 33: Operating Principle

    • Pump Overview Operating Principle The pump can generate pressures up to 151 MPa with ultra-low pulsation. The pump is based on a two-channel, dual-piston design with electronic compressibility compensation. The picture illustrates how the pump operates: Figure 1: Operating Principle Description Solvent degasser Solvent selectors...
  • Page 34 • Pump Overview The pump uses independent piston drives and highly precise position and pressure sensors. This leads to high accuracy, increased reliability, and ultra-low pulsation when delivering compressible liquids. The flow rate at atmospheric pressure is always kept constant. The vacuum degasser enhances the flow stability and allows best detector sensitivity by continuously removing air that may be trapped in the solvents.

  • Page 35: Interior Components

    • Pump Overview Interior Components The user-accessible components of the pump are located directly behind the front doors: Figure 2: Interior view Description Keypad with buttons for pump control Pump heads Tubing clips for routing solvent lines A1 to A3 to the solvent selector Solvent selectors Leak sensor Type label, indicating the part number, module name, and serial number...

  • Page 36: Piston Seal Wash System

    • Pump Overview Piston Seal Wash System To keep the pistons wet and rinse the piston seals, the pump has an active piston seal wash system (seal wash system). Piston seal washing prolongs the lifetime of the pistons and seals by preventing crystallization and particle deposits on the piston surfaces and seals, which may otherwise damage the pistons and seals.

  • Page 37: Purge Unit

    • Pump Overview Purge Unit The purge unit combines the solvent streams from the pump heads, connects the system pressure sensor to the flow system, and allows easy and automatic purging. The pump is shipped from the manufacturing site with the capillaries and tubing installed to the purge unit. Figure 4: Connections on purge unit Description Capillary from left pump head...

  • Page 38: Leak Detection

    • Pump Overview Leak Detection Leaks are a potential safety issue. The leak sensor inside the pump monitors the pump for liquid leaks from the flow connections. The liquid is collected in the leak tray and guided to the drain port. From the drain port, the liquid is discharged to waste through the drain system of the Vanquish system.

  • Page 39: Operation

    • Pump Overview Operation The pump is designed to be operated from a computer configured with the Chromeleon Chromatography Data System (CDS). The Chromeleon software provides complete instrument control, data acquisition, and data management. For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.
  • Page 40 • Pump Overview Page 40 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 41: Unpacking And Transport

    • Unpacking and Transport 4 Unpacking and Transport This chapter provides information for unpacking and moving the pump and informs you about the scope of delivery. Vanquish Pumps (VH-P10) Operating Manual Page 41...

  • Page 42: Unpacking And Moving

    • Unpacking and Transport Unpacking and Moving Damaged Packaging, Defective on Arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the pump for any signs of mechanical damage that might have occurred during shipment. If you suspect that the pump may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
  • Page 43 • Unpacking and Transport Figure 5: Carrying handles on the pump Component Carrying handles Attachment screw (one on each carrying handle) 4. Place the pump on a stable surface. 5. If applicable Remove any additional packing material. Leave any protective films attached to the surfaces of the pump until it is properly positioned in the system stack.
  • Page 44 • Unpacking and Transport 9. Some surfaces including the doors of the pump are covered by a protective film during shipment. Remove the protective film from all surfaces as applicable. Moving the Pump after Installation If you have to move the pump after it has been set up and installed in the Vanquish system, prepare the pump for transport and move it to the new location.

  • Page 45: Scope Of Delivery

    • Unpacking and Transport Scope of Delivery The following items are included in the delivery: • Pump • Ship Kit For details about the kit content, see section 10.2 Ship Kit, page 209. • Operating manual • Power cord Vanquish Pumps (VH-P10) Operating Manual Page 45...
  • Page 46 • Unpacking and Transport Page 46 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 47: Installation

    • Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the pump in the Vanquish system and in the chromatography software. Vanquish Pumps (VH-P10) Operating Manual Page 47...

  • Page 48: Safety Guidelines For Installation

    • Installation Safety Guidelines for Installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented section 2.3 Safety Precautions (see page 22). CAUTION—Heavy Load, Bulky Device The pump is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the pump, observe the following guidelines: •...

  • Page 49: Installing The Pump

    • Installation Installing the Pump The Vanquish system is installed and set up by a Thermo Fisher Scientific service engineer, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified.
  • Page 50 • Installation ♦ In the Chromeleon 6.8 Browser: Click Qualification > Instruments IQ. Follow the instructions in the Instruments Installation Qualification Operating Instructions. The manual provides information about the required materials and detailed instructions. NOTICE If the pump is operated with another data system, refer to the documentation for the software that you are using and/or perform the qualification manually.

  • Page 51: Site Requirements

    • Installation Site Requirements The operating environment is important to ensure optimal performance of the pump. This section provides important requirements for the installation site. Note the following: • Operate the pump only under appropriate laboratory conditions. • The pump is intended to be part of the Vanquish system. Observe the site requirements for the Vanquish system as stated in the Vanquish System Operating Manual.
  • Page 52 • Installation Power Cord The power cords are designed to match the wall socket requirements of the country in which they are used. The end of the power cords that plugs into the power socket on the device is identical for all power cords. The end of the power cords that plugs into the wall socket is different.

  • Page 53: Accessing The Interior Components

    • Installation Accessing the Interior Components To access the interior components in the pump, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the pump are located directly behind the doors. Figure 7: Opening the pump doors Vanquish Pumps (VH-P10) Operating Manual Page 53...

  • Page 54: Setting Up The Hardware

    • Installation Setting Up the Hardware This section describes how to set up the hardware and provides information about the pump connectors and cables. 5.5.1 System Arrangement The pump is part of the Vanquish system. The system modules are typically arranged in a system stack, with the arrangement depending on the system configuration.
  • Page 55 • Installation Description Autosampler Pump System Base Column Compartment For instructions how to set up the system stack, refer to the Vanquish System Operating Manual. Vanquish Pumps (VH-P10) Operating Manual Page 55...

  • Page 56: Connecting The Pump

    • Installation 5.5.2 Connecting the Pump Pump Connectors The following connectors are provided on the pump: Figure 9: Electrical and device connectors on the pump Description Rating plate, indicating the serial number, part number, module name, line and fuse rating, and the manufacturer's address Main power switch (on/off control) Fuse holder Power-inlet connector...
  • Page 57 • Installation Description USB hub ("A" type connector) - Allows connection to other modules in the Vanquish system USB (Universal Serial Bus) port ("B" type connector) - Allows connection to other modules in the Vanquish system or the computer on which the data management system is installed, such as the Chromeleon software TIP Thermo Fisher Scientific recommends using the USB ports only as described above.

  • Page 58: Setting Up The Flow Connections

    • Installation Setting Up the Flow Connections 5.6.1 General Information and Guidelines The pump is shipped from the manufacturing site with the flow connections installed between the interior components. This section describes how to set up the flow connections to and from the pump and additional flow connections if required.
  • Page 59 • Installation 1. Connect the pump to the drain system (see section 5.6.4, page 63). 2. Connect the solvent lines (see section 5.6.5, page64). 3. Set up the seal wash system (see section 5.6.6, page 69). 4. Connect the pump to the autosampler (see section 5.6.7, page 76).

  • Page 60: Guiding Capillaries And Tubing Through The System

    • Installation 5.6.2 Guiding Capillaries and Tubing Through the System Flow connections between the modules of the Vanquish system are guided through either the tubing chase in the devices or the guide holes or capillary clips of the devices. Tubing Chase, Tubing Guide, Tubing Bracket To guide certain tubes and lines (solvent tubing, wash liquid tubing, detector waste line) from the top module to the bottom module in the Vanquish system stack, the stackable modules have a tubing chase on...

  • Page 61: Connecting Fittings, Capillaries, And Tubing

    • Installation Tubing brackets are available for holding the tubing in place. Slip the bracket side onto the drain pipe, and clamp the other side behind the front panel (see picture). Figure 11: Tubing bracket (left), tubing bracket installed (right) Guide Holes and Capillary Clips Guide holes and capillary clips are provided at specific positions on the system modules.
  • Page 62 • Installation Connecting Viper Capillaries 5.6.3.2 This section describes how to connect Viper™ capillaries. All Viper flow connections in the Vanquish system are designed to be finger-tight. To connect Viper capillaries with knurl, follow these steps: NOTICE • Tighten or loosen Viper capillaries only with your fingers. Do not use tools other than the knurl that comes with the capillary.

  • Page 63: Guiding Liquids To Waste

    • Installation 5.6.4 Guiding Liquids to Waste The following liquids leave the pump through the outlets at the bottom right of the leak tray: • Leaking liquid from the leak tray Leaking liquid collects in the leak tray and flows off through the drain port outlet into the drain system of the Vanquish system.

  • Page 64: Connecting The Solvent Lines

    • Installation 5.6.5 Connecting the Solvent Lines The pump is shipped from the manufacturing site with the solvent lines installed between the components in the pump. To complete the setup, install the solvent lines between the degasser inlet ports and the solvent reservoirs. Parts and tools required •...
  • Page 65 • Installation c) Screw the filter top to the filter bottom. Description Solvent line filter, bottom Filter frit Solvent line filter, top Figure 14: Assembling the solvent line filter • Remove the plugs on the degasser inlets that protect the inlet during shipment, if applicable.
  • Page 66 • Installation 2. Route the solvent lines from the degasser inlets to the solvent rack, through the tubing guides in the pump and all modules above the pump in the system stack. Observe the routing guidelines in the Guiding Capillaries and Tubing Through the System section in this manual.
  • Page 67 • Installation Description Solvent line Retaining guide Reservoir cap Cap plugs Solvent line filter Figure 17: Preparing the solvent line and reservoir cap 5. Place the solvent reservoirs in the solvent rack. Position the solvent lines straight in the tubing guides. 6.
  • Page 68 • Installation Figure 18: Installing a shut off valve in a solvent line Description Solvent line Fitting Ferrule Shut off valve Description Shut off valve Retaining guide Figure 19: Solvent line with shut off valve installed 10. Check the solvent lines across the entire flow path. Make sure that the lines are not bent, pinched, or squeezed at any point in the flow path.

  • Page 69: Setting Up The Seal Wash System

    • Installation 5.6.6 Setting Up the Seal Wash System The flow path of the seal wash system passes through the metering device head in the autosampler and the pump heads in the pump. The seal wash system comprises the following parts: •...
  • Page 70 • Installation TIP To avoid the formation of gas bubbles in the seal wash reservoir, place the reservoir in the system stack on the same level or higher than the autosampler. The setup description below assumes that the reservoir is placed in the solvent rack. Figure 20: Seal wash system in the Vanquish system Description Description...
  • Page 71 • Installation Description Description Seal wash inlet port (right tubing Wash lines from seal wash pump connector) to pump head Wash line from inlet port to Wash line from pump head to metering device head seal wash detector Wash line to seal wash pump Seal wash detector Follow these steps 1.
  • Page 72 • Installation e) Check that the right tubing connector rests on the tubing holder. If it does not, push the tubing connector onto the holder. 2. In the autosampler, the seal wash line to the pump and the seal wash inlet port (right tubing connector) are interconnected. Disconnect the seal wash line (clear silicone tubing) from the seal wash inlet port (right tubing connector).
  • Page 73 • Installation 8. Route the seal wash line through the guide hole in the solvent rack. Figure 23: Guide hole and tubing guides in the solvent rack Description Guide hole Not to be used for wash liquid line; reserved for other tubing Tubing guide for wash liquid line Not to be used for wash liquid line;...
  • Page 74 • Installation 10. Fill the seal wash reservoir with seal wash liquid. Mind the requirements outlined in the previous section. Before using a seal wash reservoir for the first time, rinse the reservoir thoroughly. Use a high-purity solvent for rinsing. 11.
  • Page 75 • Installation Flushing the Seal Wash System from Hand To flush the seal wash system from hand, follow these steps: 1. Disconnect the tubing from the seal wash detector. Description Seal wash detector Tubing connection port (port labeled in) Figure 25: Seal wash detector 2.

  • Page 76: Connecting The Pump And Autosampler

    • Installation 5.6.7 Connecting the Pump and Autosampler Parts required Capillary to connect the pump to the autosampler Follow these steps 1. Guide the connecting capillary through the guide holes in the housings of both, the pump and autosampler. Figure 26: Guide holes in the autosampler and pump (left: view from autosampler;...

  • Page 77: Turning On The Pump

    • Installation Turning On the Pump TIP Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows®...

  • Page 78: Setting Up The Pump In The Software

    • Installation Setting Up the Pump in the Software This manual assumes that the chromatography software is already installed on the data system computer and a valid license is available. For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual.

  • Page 79: Operation

    • Operation 6 Operation This chapter describes the elements for pump control, provides information for routine operation and for shutdown. Vanquish Pumps (VH-P10) Operating Manual Page 79...

  • Page 80: Introduction To This Chapter

    • Operation Introduction to this Chapter The information in this chapter assumes that the initial setup of the pump has already been completed. If this is not the case, refer to the instructions in chapter 5 Installation before proceeding (see page 47).

  • Page 81: Safety Guidelines For Operation

    • Operation Safety Guidelines for Operation When operating the pump, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented section 2.3 Safety Precautions (see page 22). CAUTION—High Luminosity of LED The high luminosity produced by the LED illuminating the inside of the pump can be harmful to the eyes.

  • Page 82: Control Elements

    • Operation Control Elements The pump is designed to be operated mainly from a computer running with the chromatography software. In addition, the following elements are available on the pump: • Keypad The keypad buttons allow you to perform certain functions directly from the pump.
  • Page 83 • Operation SELECT Pressing the button selects a pump head. The LEDs next to the button indicate which pump head is selected, with L referring to the left pump head and R referring to the right pump head. The LED is green when the pump head is selected.

  • Page 84: Status Indicators

    • Operation When the pump is connected in the Chromeleon software The button functionality is as follows when the pump is connected in the Chromeleon software: • No injection (sample) or sequence is running: All functions are available from the keypad. •...
  • Page 85 • Operation STATUS LED The STATUS LED on the keypad inside the pump provides the following information: STATUS LED Description Off (dark) The power to the pump is turned off. Green The pump is functioning properly. A problem or error has occurred. For the related message, check the Chromeleon Audit Trail.

  • Page 86: Power On/Off Control

    • Operation Power On/Off Control The power switch on the pump is the main switch for power on/off control. The main power switch is turned on during initial installation of the pump. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.

  • Page 87: Use Of Solvents And Additives

    • Operation Use of Solvents and Additives Particles entering the chromatography system can block capillaries and valves, increase wear, and damage the column or system. Especially with aqueous solvents, algae and other microorganisms can grow and deposit in the chromatography system and block the solvent line filters. Blocked capillaries or filters can cause increased or unstable system pressure.
  • Page 88 • Operation • Always use the appropriate solvent line filters as recommended by Thermo Fisher Scientific. Check the filter frits for permeability at regular intervals and replace them as necessary. • The pump is shipped with UHMW-PE piston seals. Using tetrahydrofuran, ketones, or ammonium hydroxide as solvents can cause swelling and damage the seals.

  • Page 89: Preparing The Pump For Operation

    • Operation Preparing the Pump for Operation This section gives information on any additional steps that are required to prepare the pump for operation and sample analysis. Before Operating the Pump for the First Time Prepare the pump for the first-time operation, observing the following: NOTICE Flush the system flow path thoroughly before operating the pump for the first time: •...
  • Page 90 • Operation • Make sure that the chromatography system is properly equilibrated. System equilibration should include the following operations: ♦ Purging the pump (all channels, including those not used for the application) ♦ Flushing the entire chromatography system with the starting solvent to rinse out any solvent from a previous analysis run ♦...

  • Page 91: Important Operating Parameters

    • Operation Important Operating Parameters The parameters described in this section should be considered for routine operation of the pump. You can usually access them from the Chromeleon user interface. If a parameter listed below is not available in Chromeleon, consider updating the firmware and Chromeleon version. For more information, refer to Chromeleon Help and documents.
  • Page 92 • Operation Parameter Description Flow ramps Set the flow acceleration and flow deceleration. Recommendation: Set the parameters to a value between 1/3 and factor 3 of the (column) flow rate. Flow acceleration The flow acceleration (Maximum Flow Ramp Up) determines how fast the pump starts delivering with the selected flow rate.
  • Page 93 • Operation Parameter Description Pressure limits The allowed pressure range is indicated in the dialog box for the pump in the Chromeleon 7 Instrument Configuration Manager (Chromeleon 6.8 Server Configuration). You can adapt the upper and lower pressure limits within the allowed range. •...
  • Page 94 • Operation Parameter Description Solvent composition In the dialog box for the pump in the Chromeleon 7 Instrument Configuration Manager (Chromeleon 6.8 Server Configuration), set the number of the solvents to be used with the pump. You can change the standard solvent names (%A1, %A2, %A3, %B1, %B2, %B3) as required.
  • Page 95 • Operation Parameter Description Waste level You can monitor the liquid level in the waste container when you have entered the following information: • Volume of liquid in the waste container at the beginning of a sequence • Upper limit for the liquid level in the waste container The Remain Time property for the waste reports the time left until the liquid level is expected to reach the upper limit.

  • Page 96: Purging The Pump

    • Operation Purging the Pump Purging the pump means rinsing the pump for a short time at a higher flow rate, using the selected solvents. From the purge unit, the solvent is automatically dispensed to waste. When • The pump is operated for the first time To flush out the isopropanol that is present in some components of the pump when the pump is shipped from the manufacturing site •...
  • Page 97 • Operation Setting Description Channel to be Select the channel that you want to purge and the solvent to be purged and used. For details, see further down in this section. solvent to be used To Purge the Pump Select one of the following alternatives: •...
  • Page 98 • Operation Purging the Pump from the Keypad To purge the pump from the keypad, follow these steps: 1. Select the channels that you want to purge. On the keypad, check the LEDs next to the Select button. A green LED (L or R or both) indicates that the pump head (channel) is selected.

  • Page 99: Optimizing The Performance Of The Pump

    • Operation Optimizing the Performance of the Pump This section provides information for best performance of the pump and gives hints on what you can do to optimize the performance further. 6.9.1 General Guidelines To optimize the pump performance, consider the following general guidelines: •...

  • Page 100: Gradient Delay Volume, Dwell Volume, And Ripple

    • Operation 6.9.2 Gradient Delay Volume, Dwell Volume, and Ripple The gradient delay volume of an HPLC system is defined as the volume of the entire flow path between the point where the solvent streams from the pump heads are combined and the point where the mobile phase enters the column.
  • Page 101 • Operation Figure 29: Dwell volume of the pump Description Solvent degasser Solvent selectors Pump heads (two pistons, parallel) Purge unit Pressure transducer Inline filter (static filter) Pump outlet (outlet of the inline filter) Dwell volume of the pump In the standard configuration, the pump has been optimized for the dwell volume and ripple.
  • Page 102 • Operation Available Mixing Systems 6.9.2.1 Each mixing system comprises a static mixer and a capillary mixer. The volumes of both mixers determine the entire volume of the mixing system. The table lists the available mixing systems: Description Mixing system, volume: 200 µL, comprising: Static mixer, volume: 150 µL Capillary mixer, volume: 50 µL Mixing system, volume: 400 µL, comprising:...
  • Page 103 • Operation Installing the Mixing System 6.9.2.2 When • For highest sensitivity when mixing ripples interfere with the detection, for example, when you use UV absorbing solvents or solvent additives that amplify mixing ripples by interaction with the stationary phase •...
  • Page 104 • Operation 4. On the filter outlet, disconnect the capillary that connects the inline filter to the autosampler injection valve. 5. Pull the inline filter out of the mounting bracket. You can remove the mounting bracket or leave it in the pump. 6.
  • Page 105 • Operation 8. Connect the capillary mixer from the mixer kit to the port labeled OUT on the purge unit and to the static mixer inlet. 9. On the static mixer outlet, reconnect the capillary to the autosampler injection valve. Figure 32: Mixing system installed and connected Description Capillary mixer on purge unit (OUT port)

  • Page 106: Installing Solvent Shut Off Valves

    • Operation 6.9.3 Installing Solvent Shut Off Valves When To shut off the solvent flow through the system at the solvent reservoir, for example, to prevent solvent from flowing through the system when you open a flow connection on the low-pressure side •...
  • Page 107 • Operation 4. Install the shut off valve: a) Cut the solvent line near the reservoir cap with a tubing cutter. Make sure that the cut is at a right angle to the length of the line. b) Onto the free end of each solvent line, slide a fitting and a ferrule.
  • Page 108 • Operation 5. Check the solvent lines across the entire flow path. Make sure that the lines are not bent, pinched, or squeezed at any point in the flow path. 6. To remove any air bubbles from the solvent lines, purge the pump. Page 108 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 109: Shutting Down The Pump

    • Operation 6.10 Shutting Down the Pump If the pump will not be operated for some time, follow the instructions in this section. TIP The Chromeleon software provides procedures for automatically preparing the chromatography system for shutdown. The procedures include, for example, operations for reducing the flow rate, reducing the temperature in temperature-controlled devices, and turning off the detector lamps.

  • Page 110: Long-Term Shutdown

    • Operation • Make sure that the temperature of the column does not exceed 40 °C. • When resuming operation, let the flow equilibrate and verify that the operating parameters for the other system modules are set as required before proceeding. 6.10.2 Long-Term Shutdown Shutting Down the Pump To interrupt operation for a longer period, follow the instructions in this...
  • Page 111 • Operation 2. Turn off the pump flow and wait until the system pressure is down to zero. 3. Disconnect the solvent lines: a) Unscrew the caps of the solvent reservoirs and remove the solvent lines together with the caps from the reservoirs. Protect the solvent lines filter from contamination with a plastic bag.
  • Page 112 • Operation Restarting the Pump To restart the pump, follow these steps: 1. Engage the peristaltic tubing in the seal wash pump. 2. Reconnect the seal wash line and the solvent lines to the appropriate reservoirs. Retighten the reservoir caps hand-tight. Verify that the retaining guide remains in the hole in the reservoir cap.

  • Page 113: Maintenance And Service

    • Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Vanquish Pumps (VH-P10) Operating Manual Page 113...

  • Page 114: Introduction To Maintenance And Service

    • Maintenance and Service Introduction to Maintenance and Service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).

  • Page 115: Safety Guidelines For Maintenance And Service

    • Maintenance and Service Safety Guidelines for Maintenance and Service When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements that are presented in section 2.3 Safety Precautions, page WARNING—High Voltage High voltages are present inside the pump that could cause an electric shock.
  • Page 116 • Maintenance and Service CAUTION—Spraying Solvent Solvents can spray when under high pressure. • Stop the pump flow prior to opening the flow path. • Wait until the system pressure is down to zero. • When opening the flow path, wear appropriate protective equipment.

  • Page 117: General Rules For Maintenance And Service

    • Maintenance and Service General Rules for Maintenance and Service For successful maintenance and service procedures, follow these rules and recommendations: • Before starting maintenance or service procedures, shut down the pump when instructed to do so. • Use only the replacement parts specifically authorized and qualified for the pump by Thermo Fisher Scientific.

  • Page 118: Routine And Preventive Maintenance

    • Maintenance and Service Routine and Preventive Maintenance Optimum pump performance, maximum uptime of the pump, and accurate results can be obtained only if the pump is in good condition and properly maintained. 7.4.1 Maintenance Plan Perform the maintenance procedures in the table on a regular basis. The frequency given in the table is a suggestion.

  • Page 119: Flushing The Pump

    • Maintenance and Service Frequency What you should do… Annually Clean the check valves in an ultrasonic bath. Replace the seal wash seals (see section 7.6.7, page 146). Inspect the pistons for particle deposits or indications of damage, for example, scratches or scores (see section 7.6.4, page 139).
  • Page 120 • Maintenance and Service • If you use water and acetonitrile or methanol for your chromatography, flushing the channels once a week is an appropriate interval. • Mobile phases containing salts, buffering agents and/or other additives are inherently prone to organic contamination and/or biodegradation.
  • Page 121 • Maintenance and Service Follow these steps 1. Turn off the pump flow and wait until the system pressure is down to zero. 2. Install the backpressure capillary on the pump outlet. Guide the free end of the capillary to the waste container. 3.

  • Page 122: Cleaning Or Decontaminating The Pump

    • Maintenance and Service 7.4.3 Cleaning or Decontaminating the Pump Cleaning and decontamination must be performed by qualified personnel wearing suitable personal protective equipment. Always observe national and local regulations. NOTICE Wipe up all liquids spilled onto the system immediately. If surfaces are exposed for longer periods, these liquids can cause damage.
  • Page 123 • Maintenance and Service NOTICE Observe the following: • Only use cleaning detergents that will not damage the surfaces of the system. • Never use sharp tools or brushes for cleaning any surfaces. • Do not use sprays for cleaning. •...

  • Page 124: Predictive Performance

    • Maintenance and Service 7.4.4 Predictive Performance The Chromeleon software supports functions for estimating the lifetime of consumables and for monitoring and recording service and qualification information about the device. These functions, which are called Predictive Performance, allow you to schedule maintenance procedures based on the actual operating and usage conditions of the device.

  • Page 125: Seal Wash System

    • Maintenance and Service Seal Wash System Follow the sequence of steps for the maintenance procedure that you want to perform: • To test the seal wash system for leakage, see the next section further down. • To replace the seal wash lines, see section 7.5.2, page 126.

  • Page 126: Replacing The Seal Wash Lines

    • Maintenance and Service 2. Insert a syringe into the open end of the tubing. 3. To allow the liquid to travel easily through the seal wash system, press down the lever of the seal wash pump and hold it in that position with one hand.
  • Page 127 • Maintenance and Service Tools required Tubing cutter (optional) Preparations 1. Unscrew the cap of the seal wash reservoir and remove the seal wash line together with the cap from the reservoir. 2. To empty the seal wash line, press down the lever of the seal wash pump.
  • Page 128 • Maintenance and Service Description Seal wash line from autosampler Peristaltic tubing Seal wash tubing to pump head a) Disconnect the peristaltic tubing from the silicone tubing. b) Press down the lever on the seal wash pump and remove the peristaltic tubing from the pump and from the tubing holders.

  • Page 129: Replacing The Seal Wash Detector

    • Maintenance and Service 4. Check the seal wash lines over the entire flow path: a) Make sure that no bending, pinching or squeezing of the seal wash lines is present at any point in the flow path. b) If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
  • Page 130 • Maintenance and Service 4. Install the new seal wash detector assembly in the pump: a) The seal wash detector and funnel come as an assembled unit. If you have to assemble both parts for any reason, push the detector into the funnel. Mind the orientation of the funnel. b) Push the assembly into the pump.

  • Page 131: Pump Head

    • Maintenance and Service Pump Head Follow the sequence of steps for the maintenance procedure that you want to perform: • To replace a pump head, see section 7.6.2, page 133. • To replace a piston, see section 7.6.3, page 136.

  • Page 132: Overview Of Pump Head Parts

    • Maintenance and Service 7.6.1 Overview of Pump Head Parts The picture shows the main pump head parts. Figure 39: Overview of pump head parts Description Inlet assembly Inlet check valves Pump head Pump head seals (3 PTFE O-ring seals) Piston seals Support rings Seal wash bodies (pump head bushing)

  • Page 133: Replacing The Pump Head

    • Maintenance and Service 7.6.2 Replacing the Pump Head When • Damage of pump head • Problems with pump performance Parts required Pump head Tools and additional items required • Hexagon wrench, size 6 • Solvent suitable for purging to flush out harmful substances •...
  • Page 134 • Maintenance and Service TIP If a piston remained in the pump, write down the installation position in the pump head (right or left) and pull the piston out. If both pistons remained in the pump, be careful not to interchange them.
  • Page 135 • Maintenance and Service ♦ New pump heads can be installed in either position when installed for the first time. They have no label on the pressure sensor cable. For easier identification during future maintenance, consider labeling them according to the installation position (L for the left pump head or R for the right pump head).

  • Page 136: Replacing The Pistons

    • Maintenance and Service 7.6.3 Replacing the Pistons When Damage of piston Parts and additional items required • Pistons • Isopropanol Preparations Remove the pump head (see section 7.6.2, page 133). Follow these steps 1. Remove the pistons from the pump head (or pump if necessary). 2.
  • Page 137 • Maintenance and Service 5. Insert the pistons into the cavities. Figure 42: Installing the pistons 6. Place the spacing tool onto the pump head. Figure 43: Installing the spacing tool and pistons Description Spacing tool Pistons 7. Push in the pistons until they contact the tool. 8.
  • Page 138 • Maintenance and Service 10. In Chromeleon, as applicable ♦ When installing a new piston a) Update the related Piston Calibration Value property with the value from the new piston: Pump Head Property to be updated For the left pump head Under Pump_Wellness_LeftBlock Left piston PistonCalibrationValue_Drv1...

  • Page 139: Cleaning The Piston

    • Maintenance and Service 7.6.4 Cleaning the Piston When When reinstalling the piston you removed Items required • Dry, lint-free tissue • Isopropanol Follow these steps 1. Inspect the piston for signs of damage. TIP When the entire piston is still black and the fingernail test does not reveal rough areas on the piston surface, the piston is in good condition.

  • Page 140: Replacing The Piston Seals Or Support Ring

    • Maintenance and Service 7.6.5 Replacing the Piston Seals or Support Ring When • Damage to piston seals, leaky seals • Unstable flow rates or baseline noise Parts required • Piston seals • Support ring Note that the support ring is not a wear part. You need not replace the support ring when you replace a piston seal.
  • Page 141 • Maintenance and Service Preparations 1. Remove the pump head (see section 7.6.2, page 133). 2. Remove the pistons (see section 7.6.3, page 136). Follow these steps 1. Remove the screws on the rear of the pump head (on the seal wash plate).
  • Page 142 • Maintenance and Service 5. Remove the piston seal. With the removal pin, push the seal-handling tool into the pump head as far as it goes in and remove the tool. The piston seal is removed together with the tool. Once the seal has been removed, it cannot be reused.
  • Page 143 • Maintenance and Service 10. Remove the tool from the pump head. The piston seal and support ring remain in the pump head. Figure 48: Pump head, piston seals, and support rings installed 11. Insert the seal wash bodies into the pump head if applicable. Mind the correct orientation.
  • Page 144 • Maintenance and Service 14. With the screwdriver, hand-tighten the screws step by step in the order shown in the picture. Figure 51: Tightening order for seal wash screws 15. Tighten the screws step by step in the given order once again. 16.

  • Page 145: Recommendations For New Piston Seals

    • Maintenance and Service 7.6.6 Recommendations for New Piston Seals When When new piston seals have been installed TIP The actions recommended in this section directly influence pump performance and the piston seal life cycle. Items required • Backpressure capillary (from Diagnostics tool kit) •...

  • Page 146: Replacing The Seal Wash Seals

    • Maintenance and Service 7.6.7 Replacing the Seal Wash Seals When • Damage to the seal wash seal, leaky seal • Once a year Parts required Seal wash seals Tools and additional items required • Screwdriver, Torx TX25 • Seal-handling tool (see Figure 44, page 140) •...
  • Page 147 • Maintenance and Service Figure 53: Removing the seal wash plate with the spacing tool Description Pump head Seal wash plate, channel side Seal wash seals Pistons 3. If the seal wash bodies are present in the seal wash plate, remove them from the plate.
  • Page 148 • Maintenance and Service 6. To facilitate seal installation, inject a few drops of isopropanol into the seal cavities on the seal wash plate (no-channel side). 7. On the seal wash plate (no-channel side), set the insertion pin of the seal-handling tool at a 90°...
  • Page 149 • Maintenance and Service 10. Place the seal wash plate onto the pump head and insert the screws. Figure 57: Pump head with seal wash plate and plate screws 11. With the screwdriver, hand-tighten the screws step by step in the order shown in the picture.

  • Page 150: Replacing The Pump Head Seals

    • Maintenance and Service 7.6.8 Replacing the Pump Head Seals When Damage to pump head seals, leaky seals Parts and tools required • Pump head seals • Tweezers Preparations 1. Remove the pump head (see section 7.6.2, page 133). 2. Remove the pistons (see section 7.6.3, page 136).
  • Page 151 • Maintenance and Service 3. Remove the seal wash bodies from the pump head if applicable. 4. Remove the pump head seals. Using tweezers facilitates the procedure for the seals labeled 1 and 3 in the picture. Figure 60: Pump head seals Description Pump head seal, O-ring 65x1.5 Pump head seal, O-ring 9x1.5...
  • Page 152 • Maintenance and Service 7. Place the seal wash plate onto the pump head and insert the screws. Figure 62: Pump head with seal wash plate and plate screws 8. With the screwdriver, hand-tighten the screws step by step in the order shown in the picture.

  • Page 153: Testing The Piston Seals For Leakage

    • Maintenance and Service 7.6.9 Testing the Piston Seals for Leakage When Pressure pulsation, shift in retention times and/or poor reproducibility can indicate possible piston seal leakage. If messages regarding piston seal leakage appear in the chromatography software, for example, "The piston seal leakage has exceeded the recommended limit (code 4121)".
  • Page 154 • Maintenance and Service 7. Depends on your observation: ♦ Liquid level in the tubing remains unchanged The piston seals seal tightly. Reconnect the seal wash tubing to the seal wash detector (port labeled in). This completes the test. ♦ Liquid level in the tubing is decreasing The seal wash seals are leaky.

  • Page 155: Check Valves

    • Maintenance and Service Check Valves Follow the sequence of steps for the maintenance procedure that you want to perform: • To replace the inlet check valves, see the next section. • To replace the outlet check valves, see section 7.7.2, page 157.
  • Page 156 • Maintenance and Service Preparations 1. To flush out harmful substances, purge the pump with an appropriate solvent. 2. Turn off the pump flow and wait until the system pressure is down to zero. Follow these steps 1. On the inlet assembly of the pump head, disconnect the tubing from the solvent selector.

  • Page 157: Replacing The Outlet Check Valves

    • Maintenance and Service 7.7.2 Replacing the Outlet Check Valves The picture shows the parts that must be removed. Description Pump head Outlet check valves U-tubes Figure 66: Replacing an outlet check valve When Damage of check valve or leaky check valve Parts required Outlet check valve Additional items required...

  • Page 158: Cleaning The Check Valves

    • Maintenance and Service 4. Install the U-tube. 5. Purge the pump using the solvent for your application. 6. Inspect the check valves and connections for indications of leakage. Tighten leaky connections. 7. Optional Test the pump for leakage (see section 7.11, page 170).

  • Page 159: Inline Filter

    • Maintenance and Service Inline Filter Follow the sequence of steps for the maintenance procedure that you want to perform: • To test the inline filter for permeability, see the next section. • To replace the inline filter or the capillary mixer (volume: 25 µL) connecting the purge unit to the filter inlet, see section 7.8.2, page...

  • Page 160: Replacing The Inline Filter Or Capillary Mixer

    • Maintenance and Service 7.8.2 Replacing the Inline Filter or Capillary Mixer When • Impaired filter permeability • Damage of inline filter or capillary mixer, which connects the purge unit to the inline filter Parts and additional items required • As applicable ♦...
  • Page 161 • Maintenance and Service 7. As applicable, reconnect the capillary mixer to the filter inlet or install a new capillary mixer. When installing a new capillary mixer, mind the direction of flow through the mixer (indicated by the arrow on the capillary mixer). 8.

  • Page 162: Mixing System

    • Maintenance and Service Mixing System Follow the sequence of steps for the maintenance procedure that you want to perform: • To test the static mixer for permeability, see the next section. • To replace the static mixer or capillary mixer, see section 7.9.2, page 163.

  • Page 163: Replacing The Static Mixer Or Capillary Mixer

    • Maintenance and Service 7.9.2 Replacing the Static Mixer or Capillary Mixer When • Impaired permeability of the static mixer • Damage of static mixer • Leaky capillary mixer Parts and additional items required • As applicable ♦ Static mixer ♦...
  • Page 164 • Maintenance and Service 7. As applicable, reconnect the capillary mixer to the static mixer inlet or install a new capillary mixer. When installing a new capillary mixer, mind the direction of flow through the capillary mixer (indicated by the arrow on the capillary mixer).

  • Page 165: Solvent Lines And Solvent Line Filters

    • Maintenance and Service 7.10 Solvent Lines and Solvent Line Filters Follow the sequence of steps for the maintenance procedure that you want to perform: • To replace solvent lines, see section 7.10.2. • To replace the solvent line filter, see section 7.10.3, page 168.
  • Page 166 • Maintenance and Service Solvent Lines From Reservoir To Degasser 7.10.2.1 When Damage or blockage of the solvent line Parts required Solvent line between solvent reservoir and degasser inlet port Additional items required Solvent suitable for purging Preparations Empty the solvent lines (see section 7.10.1, page 165).
  • Page 167 • Maintenance and Service Solvent Lines From Degasser to Selector 7.10.2.2 When Damage or blockage of the solvent line Parts and additional items required • Solvent line between degasser outlet port and solvent selector • Solvent suitable for purging Preparations Empty the solvent lines (see section 7.10.1, page 165).

  • Page 168: Replacing The Solvent Line Filter

    • Maintenance and Service 7.10.3 Replacing the Solvent Line Filter When • Damage of solvent line filter • Impaired permeability of filter frit Parts required As applicable: • Filter holder • Filter frit Preparations Turn off the pump flow and wait until the system pressure is down to zero.
  • Page 169 • Maintenance and Service 5. Slide the filter holder onto the solvent line. 6. Retighten the reservoir cap hand-tight. Verify that the retaining guide remains in the hole in the reservoir cap. If it does not, press the retaining guide into the hole to ensure that the tubing is kept in place in the cap.

  • Page 170: Testing The Pump For Leakage

    • Maintenance and Service 7.11 Testing the Pump for Leakage When After maintenance on the fluidic system of the pump, for example on the flow connections, pump head, or check valves Parts and additional items required • Fitting plug, Viper •...
  • Page 171 • Maintenance and Service ♦ Piston seals ♦ Test the piston seals for leakage. Replace leaky piston seals. ♦ If leakage is observed with new piston seals, run in the seals. Using the solvent of your application or water, operate the pump for 2 hours or longer at 35 MPa.

  • Page 172: Replacing The Main Power Fuses

    • Maintenance and Service 7.12 Replacing the Main Power Fuses When Blown fuses Parts required Fuses (2 fuses, 5AT, 230V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric Shock...
  • Page 173 • Maintenance and Service 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the pump. 5.

  • Page 174: Updating The Pump Firmware

    • Maintenance and Service 7.13 Updating the Pump Firmware When Updating the pump firmware might be required, for example, when a new firmware version is released that adds functionality or solves problems of a previous version. Items required Firmware version/Chromeleon Service Release as appropriate TIP When a new firmware version is released, the new version will be included in the next available Chromeleon Service Release.
  • Page 175 • Maintenance and Service NOTICE A firmware downgrade or incomplete firmware update may result in loss of functionality or malfunctioning of the pump. • Do not interrupt communication between the Chromeleon software and the pump at any time during the procedure. •...

  • Page 176: Replacing The Doors

    • Maintenance and Service 7.14 Replacing the Doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the related steps in this section. Parts required Replacement door Follow these steps...
  • Page 177 • Maintenance and Service 2. Slightly tilt the door to the outside, away from the housing, and remove the door. 3. To install the door, align the door with the hinges on the housing. Be careful not to clamp tubing or capillaries between the door and the enclosure.

  • Page 178: Transporting Or Shipping The Pump

    • Maintenance and Service 7.15 Transporting or Shipping the Pump If you want to transport the pump to a new location or if you need to ship the pump, first prepare the pump for transport and then move or ship the pump as required. Follow the instructions in this section. Observe the following safety guidelines: CAUTION—Heavy Load, Bulky Device The pump is too heavy or bulky for one person alone to handle safely.
  • Page 179 • Maintenance and Service 7. Install the carrying handles and remove the pump from the Vanquish system. Follow the instructions on dismounting the system stack in the Transporting or Shipping the System section of the Vanquish System Operating Manual. TIP To remove the slide-in module from the pump, follow the related steps in section 7.16 Replacing the Slide-In Module, page...
  • Page 180 • Maintenance and Service 2. If you need to return the pump to Thermo Fisher Scientific for depot repair, contact your local Thermo Fisher Scientific support organization for the appropriate procedure. CAUTION—Possible Contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel.

  • Page 181: Replacing The Slide-In Module

    • Maintenance and Service 7.16 Replacing the Slide-In Module CAUTION—Heavy Load, Bulky Device The pump is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the pump, observe the following guidelines: •...
  • Page 182 • Maintenance and Service 2. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to remove the slide-in module properly from the enclosure in the next step.

  • Page 183: Installing The Slide-In Module

    • Maintenance and Service NOTICE The packaging for the slide-in module differs from the packaging for the complete module. Shipping the slide-in module or the pump incorrectly leads to damage on the device. Always pack and ship the slide-in module and the pump in the respective, dedicated packaging.
  • Page 184 • Maintenance and Service Follow these steps 1. Unpack the slide-in module. Remove any foam spacers from the module. 2. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to insert the slide-in module properly into the enclosure in the next step.
  • Page 185 • Maintenance and Service 5. Set up the slide-in module: a) To connect the slide-in module and to set up flow connections, follow the instructions in the Installation chapter in this operating manual. b) To prepare the slide-in module for first-time operation, follow the instructions in the Preparing the Pump for Operation section in this operating manual.
  • Page 186 • Maintenance and Service Page 186 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 187: Troubleshooting

    • Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the pump. Vanquish Pumps (VH-P10) Operating Manual Page 187...

  • Page 188: General Information About Troubleshooting

    • Troubleshooting General Information about Troubleshooting The following features help you to identify and eliminate the source for problems that may arise during operation of the pump. NOTICE When the Vanquish system includes a Vanquish charged aerosol detector, the detector may affect the operation and/or performance of the pump.
  • Page 189 • Troubleshooting Chromeleon Audit Trail Messages If the device firmware detects a problem, the problem is reported to the Chromeleon software. The Chromeleon software logs information about all events related to instrument operation for the current day in an Audit Trail. The Audit Trail is named with the current date, using the format yyyymmdd.

  • Page 190: Messages

    • Troubleshooting Messages This section provides information about the messages that may appear in the Chromeleon Audit Trail during operation of the pump. For easier identification of the source for a problem, the following information may appear in front of a message: Name Description PumpModule...
  • Page 191 • Troubleshooting TIP If a message appears in the Audit Trail that is not listed in the table, write down the code and wording of the message. If you are unable to resolve the problem, contact Thermo Fisher Scientific Technical Support for assistance.
  • Page 192 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4098 Upper pressure limit Check the flow path for blockage. exceeded. If the flow path is blocked: • The column may be contaminated. Rinse or replace the column. If the problem occurs due to column ageing, it may be sufficient to increase the setting for the upper pressure limit.
  • Page 193 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4112 Self-test failed Changes in pressure have occurred during the self-test. Wait until the system because of fluctuating pressure. pressure is down or depressurize the system and repeat the self-test. Code 4113 Self-test failed The pressure has not been down to zero during the self-test.
  • Page 194 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4125 Degasser malfunction The vacuum level monitoring function detected insufficient degasser vacuum. Turn the pump off and on again. Check the degasser vacuum in Chromeleon. After about 1 minute, the setting should change from NotOk to Ok. If the degasser vacuum is still insufficient, the degasser may be leaky.
  • Page 195 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4176 The pressure Follow these steps: exceeded the absolute limit. 1. Verify that the pressure sensor cables are properly connected. Reconnect the Check pressure sensor cables for cables if necessary. proper connection and flow path 2.
  • Page 196 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4212 Nonlinear pressure Start the pressure recalibration procedure (see section 8.5, page 200). sensor. Please perform pressure recalibration. Code 4213 Head pressure does First, run the self-test. If the message appears again, perform pressure not match system pressure recalibration (see section 8.5, page...

  • Page 197: Resolving Leakage

    • Troubleshooting Resolving Leakage When The leak sensor is wet. The leak sensor reports leakage. If leakage is not resolved after 3 minutes, the pump stops the flow. Preparations When resolving leakage, observe the safety guidelines and general rules for maintenance and service as presented in chapter 7 Maintenance and Service, page 113.

  • Page 198: Checking The Compression Values

    • Troubleshooting Checking the Compression Values The compression values of the pump head can provide valuable information for troubleshooting. When You observe pressure pulsation or shifts in retention time. Follow these steps 1. In Chromeleon, check the Compression values for the pump head (compression on the left side in the pump head = CompressionDrv1, on the right side = CompressionDrv2).
  • Page 199 • Troubleshooting ♦ The piston seal may be defective. Replace the piston seal. ♦ The inlet check valve may be defective. Replace the inlet check valve. Very high compression (message "Compression limit reached" ♦ and low pressure) ♦ Air bubbles may be present in the pump. Make sure that no air bubbles are present in the solvent lines and then, purge the pump.

  • Page 200: Performing Pressure Recalibration

    • Troubleshooting Performing Pressure Recalibration When Messages regarding the pump head pressure or nonlinear pressure sensor appear in chromatography software, for example: • Code 4212 Nonlinear pressure sensor. Please perform pressure recalibration. • Code 4213 Head pressure does not match system pressure signal. Execute self-test or perform pressure recalibration.

  • Page 201: Testing The Inline Filter/Static Mixer For Permeability

    • Troubleshooting Testing the Inline Filter/Static Mixer for Permeability When If you observe pressure pulsation, inconstant pressure, or high backpressure at the column and pump Additional items required • Water as solvent • Tissue Follow these steps • The pump has an inline filter installed Test the inline filter by following the steps in section 7.8.1, page 159.
  • Page 202 • Troubleshooting Page 202 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 203: Specifications

    • Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the pump. Vanquish Pumps (VH-P10) Operating Manual Page 203...

  • Page 204: Performance Specifications

    • Specifications Performance Specifications The pump performance is specified as follows: Type Specification* Operating principle Parallel dual-piston pump with independent piston drives and variable stroke volume Compressibility compensation Fully automated, independent of the composition of the mobile phase Flow range (settable) 0.001 –...
  • Page 205 • Specifications Type Specification* Materials in the flow path MP35N, DLC (diamond like carbon), titanium, zirconium oxide, sapphire, aluminum oxide, PEEK, PTFE, ECTFE, FEP, UHMW polyethylene, perfluoro-elastomer, amorphous fluoropolymer (AF) NOTICE For information about the chemical resistance of materials, refer to the technical literature. Solvent and additive information section 2.4, page 28 Safety features...

  • Page 206: Physical Specifications

    • Specifications Physical Specifications The physical conditions of the pump are specified as follows: Type Specification Range of use Indoor use only Ambient temperature 5 °C – 35 °C Ambient humidity 20% - 80% relative humidity (non-condensing) Pollution degree Power requirements 100 –...

  • Page 207: Accessories, Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10 Accessories, Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the pump and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts. Vanquish Pumps (VH-P10) Operating Manual Page 207...

  • Page 208: General Information

    • Accessories, Consumables and Replacement Parts 10.1 General Information The pump must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.

  • Page 209: Ship Kit

    • Accessories, Consumables and Replacement Parts 10.2 Ship Kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. See the content list included in the kit for the most recent information about the kit content at the time when the pump is shipped.

  • Page 210: Optional Accessories

    • Accessories, Consumables and Replacement Parts 10.3 Optional Accessories Item Remarks Part No. Solvent shut off valve To prevent the solvent from flowing through the system, for 6036.0010 example, when opening a flow connection on the low- pressure side Mixing systems •...

  • Page 211: Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10.4 Consumables and Replacement Parts Pump head and components Description Part No. Pump head (complete assembly), including spacing tool 6044.1200 Piston seal (RP) and seal wash seal (pack of 2 each) 6266.0309 Support ring 6040.0012 Piston, sapphire 6267.0050...
  • Page 212 • Accessories, Consumables and Replacement Parts Solvent lines, solvent line filters, waste tubing Description Part No. Solvent lines to connect the degasser inlet ports to the solvent 6036.1701 reservoirs Tubing kit, including: 6044.2050 • Solvent lines from degasser to solvent selector •...
  • Page 213 • Accessories, Consumables and Replacement Parts Seal wash system Description Part No. Seal wash detector (drop detector) and funnel 6044.1898 Peristaltic and wash tubing kit 6044.1150 The kit includes peristaltic tubing (PharMed), silicone tubing, and tubing connectors for use in •...
  • Page 214 • Accessories, Consumables and Replacement Parts Miscellaneous cables, capillaries and tubing Description Part No. Cable, mini-DIN, 6-pin, cable length: 5 m 6000.1004 USB cables, type A to type B, high-speed, USB 2.0 • 6035.9035 Cable length: 1 m 6911.0002 • Cable length: 5 m Capillary kit, system pressure sensor (P-Sys) 6044.1933...
  • Page 215 • Accessories, Consumables and Replacement Parts Description Part No. Power cord, UK 6000.1020 Power cord, USA 6000.1001 Power cord, Switzerland 6000.1030 Vanquish Pumps (VH-P10) Operating Manual Page 215...
  • Page 216 • Accessories, Consumables and Replacement Parts Page 216 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 217: Appendix

    • Appendix 11 Appendix This chapter provides additional information about compliance and the use of the digital I/O ports. Vanquish Pumps (VH-P10) Operating Manual Page 217...

  • Page 218: Compliance Information

    • Appendix 11.1 Compliance Information 11.1.1 Declarations of Conformity CE Declaration of Conformity The device has satisfied the requirements for the CE mark and is compliant with the applicable requirements. cTUVus Compliance The cTUVus label on the device indicates that the device has satisfied the requirements for the cTUVus mark.

  • Page 219: Fcc Compliance

    • Appendix 11.1.3 FCC Compliance This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment.

  • Page 220: Digital I/O

    • Appendix 11.2 Digital I/O The digital I/O ports (Dig I/O) on the pump can be used to exchange digital signals with external devices. Each port provides one digital input, one relay output, and one bidirectional input/output. Figure 74: Digital I/O port Description Bidirectional input/output Relay output —...
  • Page 221 • Appendix Wire Color Signal Name Signal Level Remarks Pink Input/output Input (low active): Configure as either input or output. On: 0-0.4 V Reference potential is ground. Note Off: 2.2-5 V the following: Open Collector Output: • The maximum input voltage at the 0-5 V, 0-2 mA input must not exceed +5 V with reference to ground.
  • Page 222 • Appendix Page 222 Vanquish Pumps (VH-P10) Operating Manual...

  • Page 223: Index

    • Index 12 Index Vanquish Pumps (VH-P10) Operating Manual Page 223...
  • Page 224 • Index accessories ............207 decontamination ........... 122 optional ............210 degasser mode ............91 ship kit .............. 209 Dig I/O ............. 56, 220 additives digital I/O ............56, 220 information ............28 Dock (button) ............83 use ..............87 door air bubbles (remove) ..........
  • Page 225 • Index inline filter ............35, 159 outlet check valve permeability ............. 159 clean ..............158 replace .............. 160 replace .............. 157 InlineFilterChanged ..........124 overview (functional) ..........31 installation .............. 47 interior view ............35 Interlink ..............56 peristaltic tubing (replace) ........126 permeability inline filter ............
  • Page 226 • Index ship kit ..............209 short-term shutdown ..........109 QualificationDone ..........124 shutdown long-term ............110 short-term ............109 signal words ............18 rating plate ............. 20 slide-in module rear seal wash cycle ..........92 install ..............183 rear seal wash system ........ 36, 69, 125 remove .............
  • Page 227 • Index TFA ................99 transport ............... 178 troubleshooting............. 187 check compression values ........ 198 messages ............190 resolve leakage ..........197 tubing (install) ............61 tubing bracket ............60 tubing chase ............60 tubing guides ............60 type label..............20 Universal Serial Bus ..........
  • Page 228 www.thermofisher.com Thermo Fisher Scientific Inc. 168 Third Avenue Waltham Massachusetts 02451...

Table of Contents