Thermo Scientific Vanquish VH-P10-A-02 Operating Manual
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Thermo Scientific Vanquish VH-P10-A-02 Operating Manual

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thermoscientific
Vanquish
Pumps
VH-P10-A-02
Operating Manual
4820.4411-EN Revision 1.0
•
October 2017

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Summary of Contents for Thermo Scientific Vanquish VH-P10-A-02

  • Page 1 thermoscientific Vanquish Pumps VH-P10-A-02 Operating Manual 4820.4411-EN Revision 1.0 • October 2017...
  • Page 2 Copyright © 2017 Thermo Fisher Scientific Inc. All rights reserved. Original Operating Manual The hardware descriptions in this manual revision refer to pumps VH-P10-A-02. For the manual release history, see page 229. Trademarks MP35N is a trademark of SPS Technologies. PharMed is a trademark of Saint-Gobain Performance Plastics.
  • Page 3 Contacting Us Contacting Us There are several ways to contact us: Ordering Information For ordering information or sales support for HPLC products, contact your local Thermo Fisher Scientific sales organization. For contact information, go to Contact Us on http://www.thermofisher.com. Technical Assistance For technical support for HPLC products, contact your local Thermo Fisher Scientific support organization.
  • Page 4 Contacting Us Page 4 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 5: Table Of Contents

    Contents Contents 1 Using this Manual ................11 About this Manual ....................12 Conventions ......................13 1.2.1 Safety Messages ..................13 1.2.2 Special Notices and Informational Notes ..........13 1.2.3 Typographical Conventions ..............14 Reference Documentation ..................15 2 Safety ....................17 Safety Symbols and Signal Words .................
  • Page 6 Contents 4 Unpacking ..................41 Unpacking ......................42 Scope of Delivery ....................45 5 Installation ..................47 Safety Guidelines for Installation ................. 48 Installing the Pump ....................49 Site Requirements ....................51 Accessing the Interior Components ..............53 Setting Up the Hardware ..................54 5.5.1 System Arrangement................
  • Page 7 Contents Optimizing the Performance of the Pump ............101 6.9.1 General Guidelines ................. 101 6.9.2 Gradient Delay Volume, Dwell Volume, and Ripple ......102 6.9.3 Installing Solvent Shut Off Valves ............109 6.10 Shutting Down the Pump ..................111 6.10.1 Short-Term Shutdown (Interruption of Operation) ....... 111 6.10.2 Long-Term Shutdown ................
  • Page 8 Contents Inline Filter ......................167 7.9.1 Testing the Inline Filter for Permeability ..........167 7.9.2 Replacing the Inline Filter or Capillary Mixer ........168 7.10 Mixing System ....................170 7.10.1 Testing the Static Mixer for Permeability ..........170 7.10.2 Replacing the Static Mixer or Capillary Mixer ........171 7.11 Solvent Lines and Solvent Line Filters ..............
  • Page 9 Contents 10 Accessories, Consumables and Replacement Parts ...... 219 10.1 General Information ................... 220 10.2 Ship Kit ........................ 221 10.3 Optional Accessories ................... 222 10.4 Consumables and Replacement Parts ..............223 11 Appendix ..................227 11.1 Compliance Information ..................228 11.1.1 Declarations of Conformity ..............
  • Page 10 Contents Page 10 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 11: Using This Manual

    • Using this Manual 1 Using this Manual This chapter provides information about this manual, the conventions used throughout the manual, and the reference documentation that is available in addition to this manual. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 11...

  • Page 12: About This Manual

    • Using this Manual About this Manual This manual describes the functional features and operating principle of your Vanquish™ pump and provides instructions for installation, set up, start up, shut down, operation, maintenance and troubleshooting. The layout of this manual is designed to provide quick reference to the sections of interest to the user.

  • Page 13: Conventions

    • Using this Manual Conventions This section describes the conventions that are used throughout this manual. 1.2.1 Safety Messages The safety messages and precautionary statements in this manual appear as follows: • Safety messages or precautionary statements that apply to the entire manual and all procedures in this manual are grouped in the Safety chapter.

  • Page 14: Typographical Conventions

    • Using this Manual 1.2.3 Typographical Conventions These typographical conventions apply to the descriptions in this manual: Data Input and Output • The following appears in bold type: ♦ Input that you enter by the keyboard or that you select with the mouse ♦...

  • Page 15: Reference Documentation

    • Using this Manual Reference Documentation In addition to this operating manual, other documentation is available for reference. Hardware Documentation Additional hardware documentation includes the following: • Operating manuals for the other modules of the Vanquish system A printed version of the manual is shipped with the device. •...
  • Page 16 • Using this Manual TIP The Chromeleon Help and documents are included in the software shipment. Third-Party Documentation Refer also to the user documentation provided by the manufacturers of third-party components and materials, for example, Safety Data Sheets (SDSs). Page 16 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 17: Safety

    • Safety 2 Safety This chapter provides general and specific safety information and informs about the intended use of the pump. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 17...

  • Page 18: Safety Symbols And Signal Words

    • Safety Safety Symbols and Signal Words 2.1.1 Safety Symbols and Signal Words in This Manual This manual contains safety messages to prevent injury of the persons using the pump. The safety symbols and signal words in this manual include the following: Always be aware of the safety information.

  • Page 19: Observing This Manual

    • Safety 2.1.2 Observing this Manual Observe the following: • Before installing or operating the pump, read this manual carefully to be familiar with the pump and this manual. The manual contains important information with regard to user safety as well as use and care of the pump.

  • Page 20: Rating Plate

    • Safety 2.1.4 Rating Plate The rating plate is present on the pump near the electrical connections. The rating plate indicates the serial number, part number, module name, revision number (if any), line and fuse rating, and the manufacturer's address. TIP An additional type label on the leak tray of the pump indicates the module name, serial number, part number, and revision number (if any).

  • Page 21: Intended Use

    • Safety Intended Use The pump is intended to be part of the Vanquish system. The intended use of the Vanquish system is to analyze mixtures of compounds in sample solutions. The pump is for use by qualified personnel and in laboratory environment only. The pump and Vanquish system are intended for laboratory research use only.

  • Page 22: Safety Precautions

    • Safety Safety Precautions 2.3.1 General Safety Information All users must observe the general safety information presented in this section and all specific safety messages and precautionary statements elsewhere in this manual during all phases of installation, operation, troubleshooting, maintenance, shutdown, and transport of the pump. If the pump is used in a manner not specified by Thermo Fisher Scientific, the protection provided by the pump could be impaired.

  • Page 23: Qualification Of The Personnel

    • Safety 2.3.2 Qualification of the Personnel Observe the information below on the proper qualification of the personnel installing and/or operating the pump. Installation Only skilled personnel are permitted to install the pump and to establish the electrical connections according to the appropriate regulations. Thermo Fisher Scientific recommends always having service personnel certified by Thermo Fisher Scientific perform the installation (for brevity, referred to as Thermo Fisher Scientific service engineer).

  • Page 24: Electrical Safety Precautions

    • Safety Protective Clothing To protect you from chemical splashes, harmful liquids, or other contamination, put on appropriate protective clothing, such as a lab coat. Protective Eyewear To prevent liquids from striking your eyes, put on appropriate protective eyewear, such as safety glasses with side shields. If there is a risk of splashing liquids, put on goggles.

  • Page 25: General Residual Hazards

    • Safety 2.3.5 General Residual Hazards Pay attention to the following general residual hazards when working with the pump: WARNING—Hazardous Substances Solvents, mobile phases, samples, and reagents might contain toxic, carcinogenic, mutagenic, infectious, or otherwise harmful substances. The handling of these substances can pose health and safety risks. •...

  • Page 26: In Case Of Emergency

    • Safety WARNING—Self-Ignition of Solvents Solvents with a self-ignition temperature below 150 °C might ignite when in contact with a hot surface (for example, due to leakage in the chromatography system). Avoid the use of these solvents. WARNING—Hazardous Vapors Mobile phases and samples might contain volatile or flammable solvents.

  • Page 27: Solvent And Additive Information

    • Safety Solvent and Additive Information To protect optimal functionality of the Vanquish system, observe these recommendations on the use of solvents and additives: • The system must be used with reversed-phase (RP) compatible solvents and additives only. • Use only solvents and additives that are compatible with all parts in the flow path.
  • Page 28 • Safety NOTICE If the system flow path includes a Vanquish charged aerosol detector, observe the specific solvent and additive information for this detector. Refer to the operating manual for the Vanquish charged aerosol detector. • Follow any specific recommendations presented in other sections of this manual.

  • Page 29: Compliance Information

    • Safety Compliance Information Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations. When the device is delivered to you, it meets all pertinent electromagnetic compatibility (EMC) and safety standards as described in this manual.
  • Page 30 • Safety Page 30 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 31: Pump Overview

    • Pump Overview 3 Pump Overview This chapter introduces you to the pump and the main components. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 31...

  • Page 32: Pump Features

    • Pump Overview Pump Features The pump includes the following main features: • Two-channel pump with each channel based on a dual-piston in- parallel design • Independent drive for each piston for high accuracy and increased flexibility • Advanced Thermal Effect Compensation (ATEC) and electronic compressibility compensation (SmartFlow™) for precise and virtually pulse-free flow •...

  • Page 33: Operating Principle

    • Pump Overview Operating Principle The pump can generate pressures up to 151 MPa with ultra-low pulsation. The pump is based on a two-channel, dual-piston design with electronic compressibility compensation. The picture illustrates how the pump operates: Figure 1: Operating principle Description Solvent selector (pump inlet) Solvent degasser (vacuum degasser)
  • Page 34 • Pump Overview Each pump head comprises two cylinders that are connected in parallel. Thus, the solvent passes only one cylinder when the pump is delivering. To achieve continuous delivery, one cylinder is refilled while the other cylinder is delivering at the selected flow rate. The pump uses independent piston drives and highly precise position and pressure sensors.

  • Page 35: Interior Components

    • Pump Overview Interior Components The user-accessible components of the pump are located directly behind the front doors: Figure 2: Interior view Description Keypad with buttons for pump control Pump head, left Tubing clips for routing solvent lines to the pump heads Pump head, right Leak sensor Type label, indicating the module name, serial number, part number, and...
  • Page 36 • Pump Overview Description Purge unit Y-connector, left System pressure sensor Page 36 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 37: Piston Seal Wash System

    • Pump Overview Piston Seal Wash System To keep the pistons wet and rinse the piston seals, the pump has an active piston seal wash system (seal wash system). Piston seal washing prolongs the lifetime of the pistons and seals by preventing crystallization and particle deposits on the piston surfaces and seals, which may otherwise damage the pistons and seals.

  • Page 38: Purge Unit

    • Pump Overview Purge Unit The purge unit combines the solvent streams from the pump heads, connects the system pressure sensor to the flow system, and allows easy and automatic purging. The pump is shipped from the manufacturing site with the capillaries and tubing installed to the purge unit. Figure 4: Connections on purge unit Description Capillary from left Y-connector...

  • Page 39: Leak Detection

    • Pump Overview Leak Detection Leaks are a potential safety issue. The leak sensor inside the pump monitors the pump for liquid leaks from the flow connections. The liquid is collected in the leak tray and guided to the drain port. From the drain port, the liquid is discharged to waste through the drain system of the Vanquish system.

  • Page 40: Operation

    • Pump Overview Operation The pump is designed to be operated from a computer configured with the Chromeleon Chromatography Data System (CDS). The Chromeleon software provides complete instrument control, data acquisition, and data management. For a basic description of instrument control and automated sample analysis with the Chromeleon software, refer to the Vanquish System Operating Manual.

  • Page 41: Unpacking

    • Unpacking 4 Unpacking This chapter provides information for unpacking the pump and informs you about the scope of delivery. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 41...

  • Page 42: Unpacking

    • Unpacking Unpacking Damaged Packaging, Defective on Arrival Inspect the shipping container for signs of external damage and, after unpacking, inspect the pump for any signs of mechanical damage that might have occurred during shipment. If you suspect that the pump may have been damaged during shipment, immediately notify the incoming carrier and Thermo Fisher Scientific about the damage.
  • Page 43 • Unpacking 3. Remove the pump from the shipping container: Grasp the pump by the carrying handles. Slowly and carefully, lift the pump out of the shipping container. Figure 5: Carrying handles on the pump Component Carrying handles Attachment screw (one on each carrying handle) 4.
  • Page 44 • Unpacking TIP Keep the shipping container, the carrying handles with the attachment screws, and all packing material. These items will be needed if the pump is transported to a new location or shipped. 9. Some surfaces including the doors of the pump are covered by a protective film during shipment.

  • Page 45: Scope Of Delivery

    • Unpacking Scope of Delivery The following items are included in the delivery: • Pump • Ship Kit For details about the kit content, see section 10.2 Ship Kit, page 221. • Operating manual • Power cord Vanquish Pumps (VH-P10-A-02) Operating Manual Page 45...
  • Page 46 • Unpacking Page 46 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 47: Installation

    • Installation 5 Installation This chapter specifies the requirements for the installation site and describes how to set up, install, and configure the pump in the Vanquish system and in the chromatography software. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 47...

  • Page 48: Safety Guidelines For Installation

    • Installation Safety Guidelines for Installation Pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented section 2.3 Safety Precautions (see page 22). CAUTION—Heavy Load, Bulky Device The pump is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the pump, observe the following guidelines: •...

  • Page 49: Installing The Pump

    • Installation Installing the Pump The Vanquish system is installed and set up by a Thermo Fisher Scientific service engineer, including all modules and options or parts shipped with them. The service engineer checks that the installation is correct and that the Vanquish system and modules operate as specified.
  • Page 50 • Installation ♦ In the Chromeleon 6.8 Browser: Click Qualification > Instruments IQ. Follow the instructions in the Instruments Installation Qualification Operating Instructions. The manual provides information about the required materials and detailed instructions. NOTICE If the pump is operated with another data system, refer to the documentation for the software that you are using and/or perform the qualification manually.

  • Page 51: Site Requirements

    • Installation Site Requirements The operating environment is important to ensure optimal performance of the pump. This section provides important requirements for the installation site. Note the following: • Operate the pump only under appropriate laboratory conditions. • The pump is intended to be part of the Vanquish system. Observe the site requirements for the Vanquish system as stated in the Vanquish System Operating Manual.
  • Page 52 • Installation Power Cord The power cords are designed to match the wall socket requirements of the country in which they are used. The end of the power cords that plugs into the power socket on the device is identical for all power cords. The end of the power cords that plugs into the wall socket is different.

  • Page 53: Accessing The Interior Components

    • Installation Accessing the Interior Components To access the interior components in the pump, open the front doors. To allow easy access from the front, the user-accessible components and flow connections in the pump are located directly behind the doors. Figure 7: Opening the pump doors Vanquish Pumps (VH-P10-A-02) Operating Manual Page 53...

  • Page 54: Setting Up The Hardware

    • Installation Setting Up the Hardware This section describes how to set up the hardware and provides information about the pump connectors and cables. 5.5.1 System Arrangement The pump is part of the Vanquish system. The system modules are typically arranged in a system stack, with the arrangement depending on the system configuration.
  • Page 55 • Installation Description Pump System Base Column Compartment For instructions on how to set up the system stack, refer to the Vanquish System Operating Manual. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 55...

  • Page 56: Connecting The Pump

    • Installation 5.5.2 Connecting the Pump Pump Connectors The following connectors are provided on the pump: Figure 9: Electrical and device connectors on the pump Description Rating plate, indicating the serial number, part number, module name, revision number (if any), line and fuse rating, and the manufacturer's address Main power switch (on/off control) Fuse holder Power-inlet connector...
  • Page 57 • Installation Description Digital I/O ports (Dig I/O) Allow exchange of digital signals with external instruments Each digital I/O port provides one input, one relay output, and one bidirectional input/output. For connection and pin assignment information, see section 11.2, page 230.
  • Page 58 • Installation NOTICE Before connecting the pump to the power line, be sure that no condensation is present in the device. Condensation in the device can damage the electronics. If you suspect that condensation is present, allow the device to warm up to room temperature. This may take several hours.

  • Page 59: Setting Up The Flow Connections

    • Installation Setting Up the Flow Connections 5.6.1 General Information and Guidelines The pump is shipped from the manufacturing site with the flow connections installed between the interior components. This section describes how to set up the flow connections to and from the pump and additional flow connections if required.

  • Page 60: Guiding Capillaries And Tubing Through The System

    • Installation 1. Connect the pump to the drain system (see section 5.6.4, page 64). 2. Connect the solvent lines (see section 5.6.5, page65). 3. Set up the seal wash system (see section 5.6.6, page 69). 4. Connect the pump to the autosampler (see section 5.6.7, page 77).
  • Page 61 • Installation Figure 10: Tubing chase with tubing guides (left: view from inside, right: view from top) Use for Solvent tubing (up to three solvent lines) Solvent tubing (up to three solvent lines) Wash liquid tubing (seal wash, autosampler needle wash) Detector waste line Tubing brackets are available for holding the tubing in place.

  • Page 62: Connecting Fittings, Capillaries, And Tubing

    • Installation 5.6.3 Connecting Fittings, Capillaries, and Tubing This section provides information about how to connect and handle capillaries, fittings, and tubing. General Guidelines 5.6.3.1 When connecting capillaries and tubing, follow these general recommendations: • Use only the capillaries and tubing (for example, solvent lines or waste tubing) that are shipped with the pump or additional or spare capillaries and tubing as recommended by Thermo Fisher Scientific.
  • Page 63 • Installation Figure 12: Viper fitting with knurl Description Knurl Capillary Slot 1. Insert the Viper capillary into the connection port. 2. Tighten the connection by the knurl. TIP Note the slot in the knurl. For narrow connections, you can easily remove the knurls from neighboring capillaries through this slot and attach them again later.

  • Page 64: Guiding Liquids To Waste

    • Installation 5.6.4 Guiding Liquids to Waste The following liquids leave the pump through the outlets at the bottom right of the leak tray: • Leaking liquid from the leak tray Leaking liquid collects in the leak tray and flows off through the drain port outlet into the drain system of the Vanquish system.

  • Page 65: Connecting The Solvent Lines

    • Installation 5.6.5 Connecting the Solvent Lines The pump is shipped from the manufacturing site with the solvent lines installed between the components in the pump. To complete the setup, install the solvent lines between the pump inlet ports and the solvent reservoirs.
  • Page 66 • Installation c) Screw the filter top to the filter bottom. Description Solvent line filter, bottom Filter frit Solvent line filter, top Figure 14: Assembling the solvent line filter • On the pump inlet, remove the plug that protects the inlet during shipment, if applicable.
  • Page 67 • Installation 3. Feed the solvent lines through the guide hole in the solvent rack and fix them in the appropriate tubing guides in the solvent rack. Figure 16: Guide hole and tubing guides in the solvent rack Description Guide hole (solvent rack) Solvent lines (up to three solvent lines) Solvent lines (up to three solvent lines) Not to be used for solvent lines;...
  • Page 68 • Installation 5. Place the solvent reservoirs in the solvent rack. Position the solvent lines straight in the tubing guides. 6. Slide a filter holder onto each solvent line. 7. Fill the solvent reservoirs with solvent, observing the guidelines and recommendations in the Use of Solvents and Additives section in this manual.

  • Page 69: Setting Up The Seal Wash System

    • Installation Description Shut off valve Retaining guide Figure 19: Solvent line with shut off valve installed 10. Check the solvent lines across the entire flow path. Make sure that the lines are not bent, pinched, or squeezed at any point in the flow path.
  • Page 70 • Installation Setting Up the Seal Wash System 5.6.6.2 Parts and tools required • Seal wash reservoir assembly, including reservoir, reservoir cap, cap plugs, and retaining guide for the seal wash line • Seal wash line (silicone tubing) for connection to the seal wash pump;...
  • Page 71 • Installation Figure 20: Seal wash system in the Vanquish system Description Seal wash reservoir Seal wash lines in the autosampler (thick tubing): Wash line from reservoir to inlet port Seal wash inlet port (right tubing connector) Wash line from inlet port to metering device head Wash line to seal wash pump Metering device head Seal wash pump (peristaltic pump)
  • Page 72 • Installation Description Seal wash lines in the pump (thin tubing): Wash lines from seal wash pump to left pump head Wash line from left pump head to right pump head Wash line from right pump head to seal wash detector Seal wash detector (drop detector) Follow these steps 1.
  • Page 73 • Installation b) With your other hand, insert the tubing between the lever and the rotor and wind it around the rotor. c) Release the lever. d) Check that the peristaltic tubing is properly inserted in the tubing holders. If it is not, push the tubing into the holders. Make sure that the tubing is not pinched or squeezed in the holders.
  • Page 74 • Installation 5. Locate the seal wash line for connection from the autosampler to the seal wash reservoir. 6. Connect the seal wash line to the seal wash inlet port (right tubing connector) on the autosampler. 7. Route the seal wash line from the autosampler to the solvent rack through the tubing guides in the autosampler and all modules above the autosampler in the system stack.
  • Page 75 • Installation Description Seal wash line Retaining guide Reservoir cap Cap plugs Figure 24: Preparing the seal wash line 10. Fill the seal wash reservoir with seal wash liquid. Mind the requirements outlined in the previous section. Before using a seal wash reservoir for the first time, rinse the reservoir thoroughly.
  • Page 76 • Installation Flushing the Seal Wash System from Chromeleon To flush the seal wash system from Chromeleon, start a seal wash cycle by setting the seal wash pump to Active mode (SealWashPump = Active). During the seal wash cycle, the seal wash system is automatically filled with the seal wash liquid.

  • Page 77: Connecting The Pump And Autosampler

    • Installation 5.6.7 Connecting the Pump and Autosampler Parts required Capillary to connect the pump to the autosampler Follow these steps 1. On the connecting capillary, remove the knurls. 2. Guide the connecting capillary through the guide holes in the housings of both, the pump and autosampler.

  • Page 78: Turning On The Pump

    • Installation Turning On the Pump TIP Before turning on the power to a Vanquish system module for the first time, verify that the chromatography software is installed on the data system computer. When the power is turned on, the required USB drivers are automatically found and the Windows™...

  • Page 79: Setting Up The Pump In The Software

    • Installation Setting Up the Pump in the Software This manual assumes that the chromatography software is already installed on the data system computer and a valid license is available. For more information about setting up the Vanquish system in the software, refer to the Vanquish System Operating Manual.
  • Page 80 • Installation Page 80 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 81: Operation

    • Operation 6 Operation This chapter describes the elements for pump control, provides information for routine operation and for shutdown. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 81...

  • Page 82: Introduction To This Chapter

    • Operation Introduction to this Chapter The information in this chapter assumes that the initial setup of the pump has already been completed. If this is not the case, refer to the instructions in chapter 5 Installation before proceeding (see page 47).

  • Page 83: Safety Guidelines For Operation

    • Operation Safety Guidelines for Operation When operating the pump, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements presented section 2.3 Safety Precautions (see page 22). CAUTION—High Luminosity of LED The high luminosity produced by the LED illuminating the inside of the pump can be harmful to the eyes.

  • Page 84: Control Elements

    • Operation Control Elements The pump is designed to be operated mainly from a computer running with the chromatography software. In addition, the following elements are available on the pump: • Keypad The keypad buttons allow you to perform certain functions directly from the pump.
  • Page 85 • Operation MUTE ALARM If beeping alerts you that the pump firmware detected a problem, for example leakage, beeping continues until you turn off the beep. Pressing this button turns off the beep for the current alarm. As a standard, beeping starts again after 10 minutes if the problem is still present or if the firmware detected another problem.

  • Page 86: Status Indicators

    • Operation The LEDs next to the button indicate the following: Description Off (dark) The pump flow is off or zero. Green The pump head is delivering. Green, flashing The pistons are undocked. When the pump is connected in the Chromeleon software The button functionality is as follows when the pump is connected in the Chromeleon software: •...
  • Page 87 • Operation LED Bar Description Green The pump is equilibrated, but no data acquisition is running. The pump flow is on. Blue A sample or sequence is running, including data acquisition. A problem or error has occurred. For the related message, check the Chromeleon Audit Trail.

  • Page 88: Power On/Off Control

    • Operation Power On/Off Control The power switch on the pump is the main switch for power on/off control. The main power switch is turned on during initial installation of the pump. For easier handling, you can use the power button on the front left of the Vanquish system base (system power button) for power on/off.

  • Page 89: Use Of Solvents And Additives

    • Operation Use of Solvents and Additives Particles entering the chromatography system can block capillaries and valves, increase wear, and damage the column or system. Especially with aqueous solvents, algae and other microorganisms can grow and deposit in the chromatography system and block the solvent line filters. Blocked capillaries or filters can cause increased or unstable system pressure.
  • Page 90 • Operation • Always use the appropriate solvent line filters as recommended by Thermo Fisher Scientific. Check the filter frits for permeability at regular intervals and replace them as necessary. • The pump is shipped with UHMW-PE piston seals. Using tetrahydrofuran, ketones, or ammonium hydroxide as solvents can cause swelling and damage the seals.

  • Page 91: Preparing The Pump For Operation

    • Operation Preparing the Pump for Operation This section gives information on any additional steps that are required to prepare the pump for operation and sample analysis. Before Operating the Pump for the First Time Prepare the pump for the first-time operation, observing the following: NOTICE Flush the system flow path thoroughly before operating the pump for the first time: •...
  • Page 92 • Operation • Verify that the doors of the modules in the Vanquish system are closed. • Make sure that the chromatography system is properly equilibrated. System equilibration should include the following operations: ♦ Purging the pump (all channels, including those not used for the application) ♦...

  • Page 93: Important Operating Parameters

    • Operation Important Operating Parameters The parameters described in this section should be considered for routine operation of the pump. You can usually access them from the Chromeleon user interface. If a parameter listed below is not available in Chromeleon, consider updating the firmware and Chromeleon version. For more information, refer to Chromeleon Help and documents.
  • Page 94 • Operation Parameter Description Flow ramps Set the flow acceleration and flow deceleration. Recommendation: Set the parameters to a value between 1/3 and factor 3 of the (column) flow rate. Flow acceleration The flow acceleration (Maximum Flow Ramp Up) determines how fast the pump starts delivering with the selected flow rate.
  • Page 95 • Operation Parameter Description Pressure limits The allowed pressure range is indicated in the dialog box for the pump in the Chromeleon 7 Instrument Configuration Manager (Chromeleon 6.8 Server Configuration). You can adapt the upper and lower pressure limits within the allowed range. •...
  • Page 96 • Operation Parameter Description Solvent composition In the dialog box for the pump in the Chromeleon 7 Instrument Configuration Manager (Chromeleon 6.8 Server Configuration), set the number of the solvents to be used with the pump. You can change the standard solvent names (%A1, %A2, %A3, %B1, %B2, %B3) as required.
  • Page 97 • Operation Parameter Description Waste level You can monitor the liquid level in the waste container when you have entered the following information: • Volume of liquid in the waste container at the beginning of a sequence • Upper limit for the liquid level in the waste container The Remain Time property for the waste reports the time left until the liquid level is expected to reach the upper limit.

  • Page 98: Purging The Pump

    • Operation Purging the Pump Purging the pump means rinsing the pump for a short time at a higher flow rate, using the selected solvents. From the purge unit, the solvent is automatically dispensed to waste. When • The pump is operated for the first time To flush out the isopropanol that is present in some components of the pump when the pump is shipped from the manufacturing site •...
  • Page 99 • Operation Setting Description Channel to be Select the channel that you want to purge and the solvent to be purged and used. For details, see further down in this section. solvent to be used To Purge the Pump Select one of the following alternatives: •...
  • Page 100 • Operation Purging the Pump from the Keypad To purge the pump from the keypad, follow these steps: 1. Select the channels that you want to purge. On the keypad, check the LEDs next to the Select button. A green LED (L or R or both) indicates that the pump head (channel) is selected.

  • Page 101: Optimizing The Performance Of The Pump

    • Operation Optimizing the Performance of the Pump This section provides information for best performance of the pump and gives hints on what you can do to optimize the performance further. 6.9.1 General Guidelines To optimize the pump performance, consider the following general guidelines: •...

  • Page 102: Gradient Delay Volume, Dwell Volume, And Ripple

    • Operation 6.9.2 Gradient Delay Volume, Dwell Volume, and Ripple The gradient delay volume of an HPLC system is defined as the volume of the entire flow path between the point where the gradient is formed and the point where the mobile phase enters the column. Thus, the gradient delay volume of an HPLC system is the volume that the pump must deliver until a change in solvent composition reaches the column inlet.
  • Page 103 • Operation Figure 29: Dwell volume of the pump Description Solvent selector Solvent degasser Pump head, left (two pistons, parallel) Y-connector, left Pump head, right (two pistons, parallel) Y-connector, right Purge unit System pressure sensor Inline filter (static filter) Pump outlet (outlet of the inline filter) Dwell volume of the pump In the standard configuration, the pump has been optimized for the dwell volume and ripple.
  • Page 104 • Operation Available Mixing Systems 6.9.2.1 Each mixing system comprises a static mixer and a capillary mixer. The volumes of both mixers determine the entire volume of the mixing system. The table lists the available mixing systems: Description Mixing system, volume: 200 µL, comprising: Static mixer, volume: 150 µL Capillary mixer, volume: 50 µL Mixing system, volume: 400 µL, comprising:...
  • Page 105 • Operation Installing the Mixing System 6.9.2.2 When • For highest sensitivity when mixing ripples interfere with the detection, for example, when you use UV absorbing solvents or solvent additives that amplify mixing ripples by interaction with the stationary phase •...
  • Page 106 • Operation 5. On the filter outlet, disconnect the capillary that connects the inline filter to the autosampler injection valve. 6. Pull the inline filter out of the mounting bracket. You can remove the mounting bracket or leave it in the pump. 7.
  • Page 107 • Operation Description Mounting holes for mounting bracket Mounting bracket Mounting screws (Torx) 8. Insert the static mixer into the mounting bracket. Mind the direction of flow through the mixer (indicated by the arrow on the static mixer). Description Static mixer inlet Static mixer outlet Figure 32: Static mixer 9.
  • Page 108 • Operation 11. Reinstall the Y-connector. 12. Reconnect the cable of seal wash detector to the DROP DET port. 13. Reconnect the pump head cable to P-WORK port if applicable. 14. Apply the flow rate of your application and have the pump deliver for a short time.

  • Page 109: Installing Solvent Shut Off Valves

    • Operation 6.9.3 Installing Solvent Shut Off Valves When To shut off the solvent flow through the system at the solvent reservoir, for example, to prevent solvent from flowing through the system when you open a flow connection on the low-pressure side •...
  • Page 110 • Operation 5. Install the shut off valve: a) Cut the solvent line near the reservoir cap with a tubing cutter. Make sure that the cut is at a right angle to the length of the line. b) Onto the free end of each solvent line, slide a fitting and a ferrule.

  • Page 111: Shutting Down The Pump

    • Operation 6.10 Shutting Down the Pump If the pump will not be operated for some time, follow the instructions in this section. TIP The Chromeleon software provides procedures for automatically preparing the chromatography system for shutdown. The procedures include, for example, operations for reducing the flow rate, reducing the temperature in temperature-controlled devices, and turning off the detector lamps.

  • Page 112: Long-Term Shutdown

    • Operation 6.10.2 Long-Term Shutdown Shutting Down the Pump To interrupt operation for a longer period, follow the instructions in this section. TIP Shutting down the pump affects the operation of the system. When shutting down the pump, also observe the shutting down instructions for the other Vanquish system modules and take appropriate action (refer to the Operating Manuals for the modules).
  • Page 113 • Operation 4. Disconnect the solvent lines: a) Unscrew the caps of the solvent reservoirs and remove the solvent lines together with the caps from the reservoirs. Protect the solvent lines filter from contamination with a plastic bag. b) Purge the pump until the solvent lines are empty. c) Disconnect the solvent lines on the pump inlets.
  • Page 114 • Operation Restarting the Pump To restart the pump, follow these steps: 1. Engage the peristaltic tubing in the seal wash pump. 2. Reconnect the seal wash line and the solvent lines to the appropriate reservoirs. Retighten the reservoir caps hand-tight. Verify that the retaining guide remains in the hole in the reservoir cap.

  • Page 115: Maintenance And Service

    • Maintenance and Service 7 Maintenance and Service This chapter describes the routine maintenance and the service procedures that the user may perform. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 115...

  • Page 116: Introduction To Maintenance And Service

    • Maintenance and Service Introduction to Maintenance and Service This chapter describes the routine maintenance and service and repair procedures that the user may perform. Additional maintenance or service procedures must be performed only by service personnel certified by Thermo Fisher Scientific (for brevity, referred to as Thermo Fisher Scientific service personnel).

  • Page 117: Safety Guidelines For Maintenance And Service

    • Maintenance and Service Safety Guidelines for Maintenance and Service When performing maintenance or service procedures, pay attention to the following safety guidelines: Observe all warning messages and precautionary statements that are presented in section 2.3 Safety Precautions, page WARNING—High Voltage High voltages are present inside the pump that could cause an electric shock.
  • Page 118 • Maintenance and Service CAUTION—Spraying Solvent Solvents can spray when under high pressure. • Stop the pump flow prior to opening the flow path. • Wait until the system pressure is down to zero. • When opening the flow path, wear appropriate protective equipment.

  • Page 119: General Rules For Maintenance And Service

    • Maintenance and Service General Rules for Maintenance and Service For successful maintenance and service procedures, follow these rules and recommendations: • Before starting maintenance or service procedures, shut down the pump when instructed to do so. • Use only the replacement parts specifically authorized and qualified for the pump by Thermo Fisher Scientific.

  • Page 120: Routine And Preventive Maintenance

    • Maintenance and Service Routine and Preventive Maintenance Optimum pump performance, maximum uptime of the pump, and accurate results can be obtained only if the pump is in good condition and properly maintained. 7.4.1 Maintenance Plan Perform the maintenance procedures in the table on a regular basis. The frequency given in the table is a suggestion.
  • Page 121 • Maintenance and Service Frequency What you should do… Annually Clean the check valves in an ultrasonic bath. Replace the seal wash seals (see section 7.7.7, page 153). Inspect the pistons for particle deposits or indications of damage, for example, scratches or scores (see section 7.7.4, page 145).

  • Page 122: Flushing The Pump

    • Maintenance and Service 7.4.2 Flushing the Pump When • To avoid contamination of the pump, see section 7.4.2.1. • With persistent contamination, see section 7.4.2.2, page 123. Avoiding Contamination 7.4.2.1 When To avoid contamination of the degassing channels Items required Fresh solvent Observe the following guidelines The following guidelines refer to conventional use of the Vanquish...
  • Page 123 • Maintenance and Service With Persistent Contamination Only 7.4.2.2 When TIP With conventional use of the Vanquish system as recommended in this manual, regularly flushing the degassing channels is usually sufficient (see the previous section in this manual). With persistent contamination, for example, if ghost peaks appear reproducibly in your blank chromatogram without injecting a sample, consider a harsher cleaning procedure.

  • Page 124: Cleaning Or Decontaminating The Pump

    • Maintenance and Service 8. For 2 hours, apply the flow rate of your application and flush the pump with fresh acetonitrile. 9. Connect the new solvent reservoirs, which contain fresh solvent. 10. Turn off the pump flow and wait until the system pressure is down to zero.
  • Page 125 • Maintenance and Service CAUTION—Explosive Gas Mixtures from Alcoholic Cleaning Detergents Alcohol-containing cleaning detergents may form flammable and explosive gas mixtures when exposed to air. • Use such cleaning detergents only when required and only in adequately ventilated rooms. • Avoid open flames or exposure to excessive heat during the cleaning process.

  • Page 126: Predictive Performance

    • Maintenance and Service 3. Wipe the cleaned surfaces with purified water to ensure that all cleaning detergent residues have been removed. 4. Wipe the surfaces dry using a soft, lint-free cloth or wipe. 7.4.4 Predictive Performance The Chromeleon software supports functions for estimating the lifetime of consumables and for monitoring and recording service and qualification information about the device.
  • Page 127 • Maintenance and Service • PistonsChanged For each pump head, a common counter is available for both pistons. • SealsChanged For each pump head, a common counter is available for all piston seals in the pump head. • QualificationDone • ServiceDone Vanquish Pumps (VH-P10-A-02) Operating Manual Page 127...

  • Page 128: Seal Wash System

    • Maintenance and Service Seal Wash System Follow the sequence of steps for the maintenance procedure that you want to perform: • To test the seal wash system for leakage, see section 7.5.1. • To replace the seal wash lines, see section 7.5.2, page 129.

  • Page 129: Replacing The Seal Wash Lines

    • Maintenance and Service 2. Insert a syringe into the open end of the tubing. 3. To allow the liquid to travel easily through the seal wash system, press down the lever of the seal wash pump and hold it in that position with one hand.
  • Page 130 • Maintenance and Service Tools required Tubing cutter (optional) Preparations 1. Unscrew the cap of the seal wash reservoir and remove the seal wash line together with the cap from the reservoir. 2. To empty the seal wash line, press down the lever of the seal wash pump.
  • Page 131 • Maintenance and Service Description Peristaltic tubing Seal wash tubing to pump head a) Disconnect the peristaltic tubing from the silicone tubing. b) Press down the lever on the seal wash pump and remove the peristaltic tubing from the pump and from the tubing holders.

  • Page 132: Replacing The Seal Wash Detector

    • Maintenance and Service 4. Check the seal wash lines over the entire flow path: a) Make sure that no bending (kink), pinching or squeezing of the seal wash lines is present at any point in the flow path. b) If you have to cut tubing to length, use a tubing cutter. Make sure that the cut is at right angle to the length of the line.
  • Page 133 • Maintenance and Service 4. Install the new seal wash detector assembly in the pump: a) The seal wash detector and funnel come as an assembled unit. If you have to assemble both parts for any reason, push the detector into the funnel. Mind the orientation of the funnel. b) Push the assembly into the pump.

  • Page 134: Y-Connector

    • Maintenance and Service Y-Connector When Damage of Y-connector Parts required Y-connector Tools and additional items required • Solvent suitable for purging to flush out harmful substances • Solvent suitable for your application Preparations 1. To flush out harmful substances, purge the pump with an appropriate solvent.
  • Page 135 • Maintenance and Service 2. Install the new Y-connector: First, connect the Y-connector capillaries to the outlet check valves. Then, connect the capillary to the purge unit. 3. Purge the pump using the solvent for your application. 4. Inspect the flow connections of the Y-connector for indications of leakage.

  • Page 136: Pump Head

    • Maintenance and Service Pump Head Follow the sequence of steps for the maintenance procedure that you want to perform: • To replace a pump head, see section 7.7.2, page 138. • To replace a piston, see section 7.7.3, page 142.

  • Page 137: Overview Of Pump Head Parts

    • Maintenance and Service 7.7.1 Overview of Pump Head Parts The picture shows the main pump head parts. Figure 41: Overview of pump head parts Description Inlet assembly Inlet check valves Pump head Pump head seals (3 PTFE O-ring seals) Piston seals Support rings Seal wash bodies (pump head bushing)

  • Page 138: Replacing The Pump Head

    • Maintenance and Service 7.7.2 Replacing the Pump Head When • Damage of pump head • Problems with pump performance Parts required Pump head Tools and additional items required • Hexagon wrench, size 6 • Solvent suitable for purging to flush out harmful substances •...
  • Page 139 • Maintenance and Service 8. Hold the pump head with one hand, remove the pump head screw, and then remove the pump head by pulling it toward you. The pistons are usually removed from the pump together with the pump head. TIP If a piston remained in the pump, write down the installation position in the pump head (right or left) and pull the piston out.
  • Page 140 • Maintenance and Service Description Spacing tool Seal wash plate Pistons ♦ New pump heads can be installed in either position when installed for the first time. They have no label on the pressure sensor cable. For easier identification during future maintenance, consider labeling them according to the installation position (L for the left pump head or R for the right pump head).
  • Page 141 • Maintenance and Service a) From Chromeleon, run the pump-specific Performance Qualification tests (PQ tests) (= Flow Precision Test and Gradient Accuracy Test). For details, refer to the Operational and Performance Qualifications manual. b) In Chromeleon, execute the QualificationDone command. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 141...

  • Page 142: Replacing The Pistons

    • Maintenance and Service 7.7.3 Replacing the Pistons When Damage of piston Parts and additional items required • Pistons • Isopropanol Preparations Remove the pump head (see section 7.7.2, page 138). Follow these steps 1. Remove the pistons from the pump head (or pump if necessary). 2.
  • Page 143 • Maintenance and Service 5. Insert the pistons into the cavities. Figure 44: Installing the pistons 6. Place the spacing tool onto the pump head. Figure 45: Installing the spacing tool and pistons Description Spacing tool Pistons 7. Push in the pistons until they contact the tool. 8.
  • Page 144 • Maintenance and Service 10. In Chromeleon, as applicable ♦ When installing a new piston a) Update the related Piston Calibration Value property with the value from the new piston: Pump Head Property to be updated For the left pump head Under Pump_Wellness_LeftBlock Left piston PistonCalibrationValue_Drv1...

  • Page 145: Cleaning The Piston

    • Maintenance and Service 7.7.4 Cleaning the Piston When When reinstalling the piston you removed Items required • Dry, lint-free tissue • Isopropanol Follow these steps 1. Inspect the piston for signs of damage. 2. Carefully rinse the piston, preferably using isopropanol. 3.

  • Page 146: Replacing The Piston Seals Or Support Ring

    • Maintenance and Service 7.7.5 Replacing the Piston Seals or Support Ring When • Damage to piston seals, leaky seals • Unstable flow rates or baseline noise Parts required • Piston seals • Support ring Note that the support ring is not a wear part. You need not replace the support ring when you replace a piston seal.
  • Page 147 • Maintenance and Service Preparations 1. Remove the pump head (see section 7.7.2, page 138). 2. Remove the pistons (see section 7.7.3, page 142). Follow these steps 1. Remove the screws on the rear of the pump head (on the seal wash plate).
  • Page 148 • Maintenance and Service 5. Remove the piston seal. With the removal pin, push the seal-handling tool into the pump head as far as it goes in and remove the tool. The piston seal is removed together with the tool. Once the seal has been removed, it cannot be reused.
  • Page 149 • Maintenance and Service 9. With the insertion pin, push the seal-handling tool into the pump head as far as it goes in. 10. Remove the tool from the pump head. The piston seal and support ring remain in the pump head. Figure 50: Pump head, piston seals, and support rings installed 11.
  • Page 150 • Maintenance and Service 13. Place the seal wash plate onto the pump head and insert the screws. Figure 52: Pump head with seal wash plate and plate screws 14. With the screwdriver, hand-tighten the screws step by step in the order shown in the picture.

  • Page 151: Recommendations For New Piston Seals

    • Maintenance and Service 22. Recommended Perform performance qualification for the pump: a) From Chromeleon, run the pump-specific Performance Qualification tests (PQ tests) (= Flow Precision Test and Gradient Accuracy Test). For details, refer to the Operational and Performance Qualifications manual. b) In Chromeleon, execute the QualificationDone command.
  • Page 152 • Maintenance and Service 5. Remove the backpressure capillary from the pump outlet and reconnect the pump to the system. 6. Purge the pump using the solvent for your application. 7. If an increased leakage rate is observed with new piston seals after several hours of operation, operate the pump for at least 2 hours at 35 MPa.

  • Page 153: Replacing The Seal Wash Seals

    • Maintenance and Service 7.7.7 Replacing the Seal Wash Seals When • Damage to the seal wash seal, leaky seal • Once a year Parts required Seal wash seals Tools and additional items required • Screwdriver, Torx TX25 • Seal-handling tool (see Figure 46, page 146) •...
  • Page 154 • Maintenance and Service Description Pump head Opening on seal wash plate Spacing tool Seal wash plate Figure 54: Removing the seal wash plate with the spacing tool Figure 55: Pump head, seal wash plate and pistons removed Description Pump head Seal wash plate, channel side Seal wash seals Pistons...
  • Page 155 • Maintenance and Service 4. Remove the seal wash seal. On the seal wash plate (channel side), set the insertion pin of the seal-handling tool at a slight angle to the seal. Push the seal out of the plate and remove the tool. Figure 56: Removing the seal wash seal 5.
  • Page 156 • Maintenance and Service 9. Insert the seal wash bodies into the pump head if applicable. Mind the correct orientation. The even side faces the pump head. Figure 58: Orientation of the seal wash body Description Side facing the pump head Side facing the seal wash plate 10.
  • Page 157 • Maintenance and Service 12. Tighten the screws step by step in the given order once again. 13. Install the pistons. Follow the related steps in section 7.7.3, page 142. 14. Install the pump head. Follow the related steps in section 7.7.2, page 138.

  • Page 158: Replacing The Pump Head Seals

    • Maintenance and Service 7.7.8 Replacing the Pump Head Seals When Damage to pump head seals, leaky seals Parts and tools required • Pump head seals • Tweezers Preparations 1. Remove the pump head (see section 7.7.2, page 138). 2. Remove the pistons (see section 7.7.3, page 142).
  • Page 159 • Maintenance and Service 3. Remove the seal wash bodies from the pump head if applicable. 4. Remove the pump head seals. Using tweezers facilitates the procedure for the seals labeled 1 and 3 in the picture. Figure 62: Pump head seals Description Pump head seal, O-ring 65x1.5 Pump head seal, O-ring 9x1.5...
  • Page 160 • Maintenance and Service 7. Place the seal wash plate onto the pump head and insert the screws. Figure 64: Pump head with seal wash plate and plate screws 8. With the screwdriver, hand-tighten the screws step by step in the order shown in the picture.

  • Page 161: Testing The Piston Seals For Leakage

    • Maintenance and Service 7.7.9 Testing the Piston Seals for Leakage When Pressure pulsation, shift in retention times and/or poor reproducibility can indicate possible piston seal leakage. If messages regarding piston seal leakage appear in the chromatography software, for example, "The piston seal leakage has exceeded the recommended limit (code 4121)".
  • Page 162 • Maintenance and Service 5. Apply a flow rate of 3 mL using isopropanol. 6. To evaluate possible leakage, observe the liquid level in the tubing. Finish the observation before a new seal wash cycle starts (after one hour). 7. Depends on your observation: ♦...

  • Page 163: Check Valves

    • Maintenance and Service Check Valves Follow the sequence of steps for the maintenance procedure that you want to perform: • To replace the inlet check valves, see the next section. • To replace the outlet check valves, see section 7.8.2, page 165.
  • Page 164 • Maintenance and Service Follow these steps 1. On the inlet assembly of the pump head, disconnect the tubing from the degasser. 2. Remove the inlet assembly: a) Set the lever on the assembly into the Unlock position. b) Pull the assembly downward. TIP Pushing down the assembly on the left side facilitates removing the assembly.

  • Page 165: Replacing The Outlet Check Valves

    • Maintenance and Service 7.8.2 Replacing the Outlet Check Valves The picture shows the parts that must be removed. Description Pump head Outlet check valves Figure 68: Replacing an outlet check valve When Damage of check valve or leaky check valve Parts required Outlet check valve Additional items required...

  • Page 166: Cleaning The Check Valves

    • Maintenance and Service 5. Inspect the check valves and connections for indications of leakage. Tighten leaky connections when the system pressure is down to zero. 6. Optional Test the pump for leakage (see section 7.12, page 178). 7.8.3 Cleaning the Check Valves When •...

  • Page 167: Inline Filter

    • Maintenance and Service Inline Filter Follow the sequence of steps for the maintenance procedure that you want to perform: • To test the inline filter for permeability, see the next section. • To replace the inline filter or the capillary mixer (volume: 25 µL) connecting the purge unit to the filter inlet, see section 7.9.2, page...

  • Page 168: Replacing The Inline Filter Or Capillary Mixer

    • Maintenance and Service 7.9.2 Replacing the Inline Filter or Capillary Mixer When • Impaired filter permeability • Damage of inline filter or capillary mixer, which connects the purge unit to the inline filter Parts and additional items required • As applicable ♦...
  • Page 169 • Maintenance and Service 7. Insert the new inline filter into the mounting bracket. Mind the direction of flow through the filter (indicated by the arrow on the filter). 8. As applicable, reconnect the capillary mixer to the filter inlet or install a new capillary mixer.

  • Page 170: Mixing System

    • Maintenance and Service 7.10 Mixing System Follow the sequence of steps for the maintenance procedure that you want to perform: • To test the static mixer for permeability, see the next section. • To replace the static mixer or capillary mixer, see section 7.10.2, page 171.

  • Page 171: Replacing The Static Mixer Or Capillary Mixer

    • Maintenance and Service 7.10.2 Replacing the Static Mixer or Capillary Mixer When • Impaired permeability of the static mixer • Damage of static mixer • Leaky capillary mixer Parts and additional items required • As applicable ♦ Static mixer ♦...
  • Page 172 • Maintenance and Service 7. Insert the new static mixer into the mounting bracket. Mind the direction of flow through the static mixer (indicated by the arrow on the static mixer). 8. As applicable, reconnect the capillary mixer to the static mixer inlet or install a new capillary mixer.

  • Page 173: Solvent Lines And Solvent Line Filters

    • Maintenance and Service 7.11 Solvent Lines and Solvent Line Filters Follow the sequence of steps for the maintenance procedure that you want to perform: • To replace solvent lines, see section 7.11.2. • To replace the solvent line filter, see section 7.11.3, page 176.
  • Page 174 • Maintenance and Service • Solvent line from the solvent reservoir to the pump inlet Follow the steps in the next section. • Solvent line from the pump inlet to the degasser Follow the steps in section 7.11.2.2, page 175. •...
  • Page 175 • Maintenance and Service Solvent Lines From Pump Inlet to Degasser 7.11.2.2 When Damage or blockage of the solvent line Parts and additional items required • Solvent line between pump inlet (solvent selector) and degasser • Solvent suitable for purging Preparations To flush out harmful substances, purge the pump with an appropriate solvent.

  • Page 176: Replacing The Solvent Line Filter

    • Maintenance and Service 7.11.3 Replacing the Solvent Line Filter When • Damage of solvent line filter • Impaired permeability of filter frit Parts required As applicable: • Filter holder • Filter frit Additional items required Solvent suitable for purging Preparations Turn off the pump flow and wait until the system pressure is down to zero.
  • Page 177 • Maintenance and Service c) Screw the filter top to the filter bottom. Description Solvent line filter, bottom Filter frit Solvent line filter, top Figure 69: Assembling the solvent line filter 5. Slide the filter holder onto the solvent line. 6.

  • Page 178: Testing The Pump For Leakage

    • Maintenance and Service 7.12 Testing the Pump for Leakage When After maintenance on the fluidic system of the pump, for example on the flow connections, pump head, or check valves TIP If you are using Chromeleon 7.2.6 and later, run the Advanced Leak Test (see section 8.3, page 207).
  • Page 179 • Maintenance and Service Possible sources for leakage: ♦ Capillary connections Inspect the capillary connections for signs of leakage. Tighten or replace leaky connections. ♦ Piston seals ♦ Test the piston seals for leakage. Replace leaky piston seals. ♦ If leakage is observed with new piston seals, run in the seals. Using the solvent of your application or water, operate the pump for 2 hours or longer at 35 MPa.

  • Page 180: Replacing The Main Power Fuses

    • Maintenance and Service 7.13 Replacing the Main Power Fuses When Blown fuses Parts required Fuses (2 fuses, 5AT, 230V AC, slow-blow, 5 x 20 mm) from Fuses Kit Tools required Slotted screwdriver, any size between 3.3 mm and 5.5 mm is appropriate Preparations WARNING—Electric Shock...
  • Page 181 • Maintenance and Service 1. Use the screwdriver to remove the fuse holder. 2. Replace the two fuses with new fuses of the specified type and current rating. Always replace both fuses. 3. Reinstall the fuse holder. 4. Reconnect the power cord to the power source and to the pump. 5.

  • Page 182: Updating The Pump Firmware

    • Maintenance and Service 7.14 Updating the Pump Firmware When Updating the pump firmware might be required, for example, when a new firmware version is released that adds functionality or solves problems of a previous version. Items required Firmware version/Chromeleon Service Release as appropriate TIP When a new firmware version is released, the new version will be included in the next available Chromeleon Service Release.
  • Page 183 • Maintenance and Service NOTICE A firmware downgrade or incomplete firmware update may result in loss of functionality or malfunctioning of the pump. • Do not interrupt communication between the Chromeleon software and the pump at any time during the procedure. •...

  • Page 184: Replacing The Doors

    • Maintenance and Service 7.15 Replacing the Doors When Damage of door TIP The maintenance procedures do not require that you remove the doors. If this should ever be required for a specific reason or procedure, follow the related steps in this section. Parts required Replacement door Follow these steps...
  • Page 185 • Maintenance and Service 4. Insert the hinges in the groove, by pushing up and slightly turning the door. 5. Push the door downward to lock it in place. You can close the door only when it is properly installed. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 185...

  • Page 186: Transporting Or Shipping The Pump

    • Maintenance and Service 7.16 Transporting or Shipping the Pump If you want to transport the pump to a new location or if you need to ship the pump, first prepare the pump for transport and then move or ship the pump as required. Follow the instructions in this section. Observe the following safety guidelines: CAUTION—Heavy Load, Bulky Device The pump is too heavy or bulky for one person alone to handle safely.

  • Page 187: Preparing The Pump For Transport

    • Maintenance and Service 7.16.1 Preparing the Pump for Transport To prepare the pump for transport, follow these steps: 1. Perform a long-term shut down of the pump. See section 6.10.2 Long-Term Shutdown, page 112. 2. Turn off the pump with its main power switch and disconnect the power cord.

  • Page 188: Transporting The Pump To A New Location

    • Maintenance and Service 7.16.2 Transporting the Pump to a New Location To transport the pump to a new location, follow these steps: 1. Observe the notes for handling and lifting the pump safely. 2. Transport the pump to the new location. 3.
  • Page 189 • Maintenance and Service CAUTION—Possible Contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel. • Decontaminate all parts of the device that you want to return for repair. • Fill in and sign the Decontamination Certificate, which is part of the Service Return Form.

  • Page 190: Replacing The Slide-In Module

    • Maintenance and Service 7.17 Replacing the Slide-In Module CAUTION—Heavy Load, Bulky Device The pump is too heavy or bulky for one person alone to handle safely. To avoid personal injury or damage to the pump, observe the following guidelines: •...
  • Page 191 • Maintenance and Service 2. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to remove the slide-in module properly from the enclosure in the next step.

  • Page 192: Installing The Slide-In Module

    • Maintenance and Service NOTICE Shipping the slide-in module improperly leads to damage on the device. Always ship the slide-in module as described in this operating manual. CAUTION—Possible Contamination Hazardous substances may have contaminated the device during operation and may cause personal injury to service personnel. •...
  • Page 193 • Maintenance and Service Follow these steps 1. Push all tubing and capillaries, which are present in the tubing chase of the Vanquish system modules, into the tubing chase. Otherwise, you will not be able to insert the slide-in module properly into the enclosure in the next step.
  • Page 194 • Maintenance and Service 4. Set up the slide-in module: a) To connect the slide-in module and to set up flow connections, follow the instructions in the Installation chapter in this operating manual. b) To prepare the slide-in module for first-time operation, follow the instructions in the Preparing the Pump for Operation section in this operating manual.

  • Page 195: Troubleshooting

    • Troubleshooting 8 Troubleshooting This chapter is a guide to troubleshooting issues that may arise during operation of the pump. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 195...

  • Page 196: General Information About Troubleshooting

    • Troubleshooting General Information about Troubleshooting The following features help you to identify and eliminate the source for problems that may arise during operation of the pump. NOTICE When the Vanquish system includes a Vanquish charged aerosol detector, the detector may affect the operation and/or performance of the pump.
  • Page 197 • Troubleshooting Chromeleon Audit Trail Messages If the device firmware detects a problem, the problem is reported to the Chromeleon software. The Chromeleon software logs information about all events related to instrument operation for the current day in an Audit Trail. The Audit Trail is named with the current date, using the format yyyymmdd.
  • Page 198 • Troubleshooting Firmware Failure If a firmware failure occurred during operation of the module, an exception log has been created about the processes during the firmware failure. The firmware sends the exception log to the Audit Trail when the module is connected in Chromeleon. In this case, observe the following: •...

  • Page 199: Messages

    • Troubleshooting Messages This section provides information about the messages that may appear in the Chromeleon Audit Trail during operation of the pump. For easier identification of the source for a problem, the following information may appear in front of a message: Name Description PumpModule...
  • Page 200 • Troubleshooting TIP If a message appears in the Audit Trail that is not listed in the table, write down the code and wording of the message. If you are unable to resolve the problem, contact Thermo Fisher Scientific Technical Support for assistance.
  • Page 201 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 91 Data changed during • This may be a temporary error. Turn off the module. Wait for 5 seconds and read. turn on the module again. • The firmware may be defective. Update the firmware, see section 7.14, page 182.
  • Page 202 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4100 Purge pressure limit A pressure of more than 5 MPa built up after a purge cycle has started. exceeded. Perform the following sequence of steps: 1. On the purge unit, remove the capillary from the system pressure sensor. 2.
  • Page 203 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4120 The rear seal wash This message alerts you only when the drop counter functionality of the seal system has run out of wash wash detector is enabled (standard setting). solution.
  • Page 204 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4152 Pressure sensor Verify that the cable of the pressure sensor for which the message appears is malfunction. Check cable properly plugged into the connection port. Restart the flow. connection and retry.
  • Page 205 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4208 System pressure too Follow these steps: high. Please relief pressure and 1. Verify that the pressure sensor cables are properly connected. Reconnect the retry. cables if necessary. 2. Check the flow path for clogging and remedy the situation if necessary. 3.
  • Page 206 • Troubleshooting Message and Code Possible Cause and Remedial Action Code 4232 The XX pump head XX is the left pump head or right pump head has been exchanged. The message appears when a pump head has been replaced or when the left and right pump heads have been interchanged, for example, accidentally during maintenance/service.

  • Page 207: Testing From Chromeleon

    • Troubleshooting Testing From Chromeleon With Chromeleon 7.2.6 and later The Chromeleon software supports functions for helping you identify and eliminate the source for a problem. A wizard guides you through the process and provides information on remedial actions. When •...

  • Page 208: Resolving Leakage

    • Troubleshooting Resolving Leakage TIP If you are using Chromeleon 7.2.6 or later and suspect that leakage may be present in the pump, run the Simple Leak Test (see section 8.3, page 207). When the leak sensor reports leakage, see further down. When The leak sensor is wet.
  • Page 209 • Troubleshooting 4. Allow the sensor to adjust to the ambient temperature for a few minutes. 5. If leakage is no longer reported, you can resume operation. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 209...

  • Page 210: Pressure Problems Or Shifts In Retention Time

    • Troubleshooting Pressure Problems or Shifts in Retention Time 8.5.1 Introduction When you observe pressure issues or shifts in retention time, the following sections help you to identify and eliminate the source for the problem: • Pressure pulsation section 8.5.2, page 211 section 8.5.4, page 214.

  • Page 211: Resolving Pressure Pulsation Or Shifts In Retention Time

    • Troubleshooting 8.5.2 Resolving Pressure Pulsation or Shifts In Retention Time When You observe pressure pulsation or shifts in retention time. Chromeleon 7.2.6 and later Run the Advanced Leak Test (see section 8.3, page 207). Chromeleon 6.8 and Chromeleon 7 earlier than Chromeleon 7.2.6 Check the compression values.
  • Page 212 • Troubleshooting 2. Take remedial action if required. Compression value lower than indicated in the table ♦ Replace the outlet check valve. ♦ Compression value higher than indicated in the table ♦ The piston seal may be defective. Replace the piston seal. ♦...

  • Page 213: Performing Pressure Recalibration

    • Troubleshooting 8.5.3 Performing Pressure Recalibration When Messages regarding the pump head pressure or nonlinear pressure sensor appear in chromatography software, for example: • Code 4212 Nonlinear pressure sensor. Please perform pressure recalibration. • Code 4213 Head pressure does not match system pressure signal. Execute self-test or perform pressure recalibration.

  • Page 214: Testing The Inline Filter/Static Mixer For Permeability

    • Troubleshooting 8.5.4 Testing the Inline Filter/Static Mixer for Permeability When If you observe pressure pulsation, inconstant pressure, or high backpressure at the column and pump Additional items required • Water as solvent • Tissue Follow these steps • The pump has an inline filter installed Test the inline filter by following the steps in section 7.9.1, page 167.

  • Page 215: Specifications

    • Specifications 9 Specifications This chapter provides the physical and performance specifications, including information about the materials used in the flow path of the pump. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 215...

  • Page 216: Performance Specifications

    • Specifications Performance Specifications The pump performance is specified as follows: Type Specification* Operating principle Parallel dual-piston pump with independent piston drives and variable stroke volume Compressibility compensation Fully automated, independent of the composition of the mobile phase Flow range (settable) 0.001 –...
  • Page 217 • Specifications Type Specification* Materials in the analytical flow path MP35N, DLC (diamond like carbon), titanium, ceramics, PEEK, UHMW PE, fluoropolymers For information about the seal wash liquid to be used, see section 5.6.6.1 Choosing the Seal Wash Liquid, page NOTICE For information about the chemical resistance of materials refer to the technical literature.

  • Page 218: Physical Specifications

    • Specifications Physical Specifications The physical conditions of the pump are specified as follows: Type Specification Range of use Indoor use only Ambient operating temperature 5 °C – 35 °C Ambient storage temperature -20 °C – 45 °C Ambient operating humidity 20% - 80% relative humidity (non-condensing) Ambient storage humidity Maximum 60% relative humidity (non-condensing)

  • Page 219: Accessories, Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10 Accessories, Consumables and Replacement Parts This chapter describes the standard accessories that are shipped with the pump and the accessories that are available as an option. This chapter also provides information for reordering consumables and replacement parts. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 219...

  • Page 220: General Information

    • Accessories, Consumables and Replacement Parts 10.1 General Information The pump must be operated only with the replacement parts and additional components, options, and peripherals specifically authorized and qualified by Thermo Fisher Scientific. Accessories, consumables, and replacement parts are always maintained at the latest technical standard.

  • Page 221: Ship Kit

    • Accessories, Consumables and Replacement Parts 10.2 Ship Kit The ship kit includes the items listed in the table. The kit content is subject to change and may vary from the information in this manual. See the content list included in the kit for the most recent information about the kit content at the time when the pump is shipped.

  • Page 222: Optional Accessories

    • Accessories, Consumables and Replacement Parts 10.3 Optional Accessories Item Remarks Part No. Solvent shut off valve To prevent the solvent from flowing through the system, for 6036.0010 example, when opening a flow connection on the low- pressure side Mixing systems •...

  • Page 223: Consumables And Replacement Parts

    • Accessories, Consumables and Replacement Parts 10.4 Consumables and Replacement Parts Inline filter Description Part No. Inline filter set, including: 6044.5018 • Inline filter (static filter, volume: 10 µL) • Capillary mixer (volume: 25 µL) to connect the purge unit to the static inline filter •...
  • Page 224 • Accessories, Consumables and Replacement Parts Miscellaneous Description Part No. Capillary kit, system pressure sensor (P-Sys) 6044.1933 The kit includes the capillaries from system pressure sensor to purge unit (short capillary) and from purge unit to system pressure sensor (long capillary). Capillary, nanoViper (length: 750 mm, inner diameter: 75 µm) 6041.5780 To be used, for example, when running in new piston seals.
  • Page 225 • Accessories, Consumables and Replacement Parts Pump head and components Description Part No. Pump head (complete assembly), including spacing tool 6044.1201 Piston seal (RP) and seal wash seal (pack of 2 each) 6266.0309 Support ring 6040.0012 Piston, sapphire 6267.0050 Inlet assembly 6044.2330 Inlet check valve 6044.2300...
  • Page 226 • Accessories, Consumables and Replacement Parts Seal wash system Description Part No. Seal wash detector (drop detector) and funnel 6044.1898 Peristaltic and wash tubing kit 6044.1150 The kit includes peristaltic tubing (PharMed), silicone tubing, and tubing connectors for use in •...

  • Page 227: Appendix

    • Appendix 11 Appendix This chapter provides additional information about compliance and the use of the digital I/O ports. Vanquish Pumps (VH-P10-A-02) Operating Manual Page 227...

  • Page 228: Compliance Information

    • Appendix 11.1 Compliance Information 11.1.1 Declarations of Conformity CE Declaration of Conformity The device has satisfied the requirements for the CE mark and is compliant with the applicable requirements. cTUVus Compliance The cTUVus label on the device indicates that the device has satisfied the requirements for the cTUVus mark.

  • Page 229: Weee Compliance

    • Appendix 11.1.2 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Figure 75: WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.

  • Page 230: Digital I/O

    • Appendix 11.2 Digital I/O The digital I/O ports (Dig I/O) on the pump can be used to exchange digital signals with external devices. Each port provides one digital input, one relay output, and one bidirectional input/output. Figure 76: Digital I/O port Description Bidirectional input/output Relay output —...
  • Page 231 • Appendix Wire Color Signal Name Signal Level Remarks Pink Input/output Input (low active): Configure as either input or output. On: 0-0.4 V Reference potential is ground. Note Off: 2.2-5 V the following: Open Collector Output: • The maximum input voltage at the 0-5 V, 0-2 mA input must not exceed +5 V with reference to ground.
  • Page 232 • Appendix Page 232 Vanquish Pumps (VH-P10-A-02) Operating Manual...

  • Page 233: Index

    • Index 12 Index Vanquish Pumps (VH-P10-A-02) Operating Manual Page 233...
  • Page 234 • Index consumables ..........219, 223 cTUVus mark ............228 curve ............... 93 accessories ............219 optional ............222 ship kit .............. 221 additives decontamination ........... 124 information ............27 degasser mode ............93 use ..............89 Dig I/O ............. 57, 230 Advanced Leak Test .......
  • Page 235 • Index operation ............40, 81 interrupt ............111 inlet check valve long-term shutdown ......... 112 clean ..............166 short-term shutdown ........111 replace .............. 163 outlet check valve inline filter ............35, 167 clean ..............166 permeability ............. 167 replace ..............
  • Page 236 • Index purge unit ............... 38 setup purge valve ............. 38 flow connections ..........59 purging ..............98 hardware ............54 software ............. 79 ship ................ 186 ship kit ..............221 QualificationDone ..........127 short-term shutdown ..........111 shutdown long-term ............112 short-term ............
  • Page 237 • Index support ring ............146 system arrangement ..........54 Viper ................ 62 system equilibration ..........91 System Interlink ............56 system power button ..........88 waste ............... 64 waste level monitoring ..........97 waste outlet port ............. 64 TFA ................ 101 WEEE ..............
  • Page 238 www.thermofisher.com Thermo Fisher Scientific Inc. 168 Third Avenue Waltham Massachusetts 02451...

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