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Thermo Scientific Accela 600 Hardware Manual

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Thermo
Accela 600 Pump and
1250 Pump
Hardware Manual
60157-97010 Revision J
December 2013

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Summary of Contents for Thermo Scientific Accela 600

  • Page 1 Thermo Accela 600 Pump and 1250 Pump Hardware Manual 60157-97010 Revision J December 2013...
  • Page 2 © 2013 Thermo Fisher Scientific Inc. All rights reserved. Exactive, Foundation, MSQ, and TSQ are trademarks, and Accela, ChromQuest, LTQ, and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc. in the United States. The following are registered trademarks in the United States and other countries: Fluorinert is a registered trademark of 3M.
  • Page 3 Thermo Fisher Scientific. To ensure continued compliance with EMC and safety standards, replacement parts and additional components, options, and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives. Accela 600 Pump EMC Directive 2004/108/EC EMC compliance has been evaluated by TÜV Rheinland of North America Inc.
  • Page 4 Accela 1250 Pump EMC Directive 2004/108/EC EMC compliance has been evaluated by TÜV Rheinland of North America Inc. EN 55011: 2007 EN 61000-4-5: 2005 EN 61000-3-2: 2006 EN 61000-4-6: 2007 EN 61000-3-3: 1995, A1: 2001, A2: 2005 EN 61000-4-11: 2004 EN 61000-4-2: 1995, A1: 1999, A2: 2001 EN 61326-1: 2006 EN 61000-4-3: 2006...
  • Page 5 Notice on Lifting and Handling of Thermo Scientific Instruments For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher Scientific instrument requires a team effort to lift and/or move the instrument.
  • Page 7 WEEE Compliance This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union (EU) Member State, and these companies should dispose of or recycle this product.
  • Page 8 Conformité DEEE Ce produit doit être conforme à la directive européenne (2002/96/EC) des Déchets d'Equipements Electriques et Electroniques (DEEE). Il est marqué par le symbole suivant: Thermo Fisher Scientific s'est associé avec une ou plusieurs compagnies de recyclage dans chaque état membre de l’union européenne et ce produit devrait être collecté...
  • Page 9 San Jose products. im Unklaren sind, setzen Sie sich, bevor Sie póngase en contacto con el personal de servicio técnico l’assistance technique pour les produits Thermo Scientific fortfahren, mit dem technischen Support für de los productos Thermo Scientific San Jose.
  • Page 10 Technical Support for Thermo Scientific im unklaren sind, setzen Sie sich, bevor Sie póngase en contacto con el personal de servicio técnico l’assistance technique pour les produits Thermo Scientific San Jose products. fortfahren, mit Ihrer lokalen technischen de los productos Thermo Scientific San Jose.
  • Page 11 す。この標識記号は機器にも表示されています。この危険の詳細につい is not included in the other categories. 危险的详细信息，参阅适当的仪器手册。若对任何步骤的安全事项有疑 ては、機器のマニュアルを参照してください。 This symbol also appears on the 问，联系 Thermo Scientific San Jose 产品的技术支持中心。 手順の安全性にご不明な点がある場合は、Thermo Scientific San Jose 製品の instrument. For details about the hazard, テクニカルサポートまでお問い合わせください。 refer to the instrument manual. When the safety of a procedure is questionable, contact Technical Support for Thermo Scientific San Jose products.
  • Page 12 作業の障害物 : 床にあるコード、ホース、その他の物体に注意してく 绊倒危险：注意地面上的线、管或其他物品。 or other objects located on the floor. ださい。 When the safety of a procedure is 手順の安全性にご不明な点がある場合は、Thermo Scientific San Jose 製品の 如对安全程序有疑问，联系 Thermo Scientific San Jose 产品的技术支持 questionable, contact Technical Support テクニカルサポートまでお問い合わせください。 中心。 for Thermo Scientific San Jose products.

  • Page 13: Table Of Contents

    Chapter 2 Accela 600 Pump or Accela 1250 Pump Installation ..... . .11 Accela Pump Installation Checklist ........12 Shipment Inspection .
  • Page 14 Error Messages ..........116 Accela 600 and 1250 Pump Hardware Manual...
  • Page 15 Accela 600 Pump Accessory Kit........
  • Page 17 Preface This manual describes how to install, maintain, and troubleshoot the Thermo Scientific™ Accela™ 600 Pump and the Accela 1250 Pump. You can operate these Accela pumps from a computer with an installation of either the ChromQuest™ or Thermo Xcalibur™ data system.

  • Page 18: Preface

    In addition to this manual, the following documents provide information about the Accela 600 Pump and the Accela 1250 Pump. You can access the manuals as PDF files from the Start menu on the computer taskbar and the Help files from data system’s Help menu.
  • Page 19 6. On the Documents page, click the link to the right of Download to view the PDF. 7. Click the Save a Copy icon, , browse to an appropriate folder, and then click Save to save a copy of the document. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 20: Safety And Special Notices

    CAUTION Highlights chemical hazards to humans, property, or the environment. Each chemical notice is accompanied by the chemical caution symbol. CAUTION Highlights eye hazards to humans. Each eye hazard notice is accompanied by the safety goggles caution symbol. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 21: Safety Information

    CAUTION To prevent personal injury, observe good laboratory practice when handling solvents, changing tube lines. or both. Consult the pertinent material safety data sheets (MSDSs) for the solvents used for HPLC analysis. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 22: Environmental Conditions

    • Use HPLC-grade or LC/MS-grade solvents • Connect the drainage tubes from the pump, autosampler, and detector to an appropriate waste receptacle. Dispose of solvents as specified by local regulations. xxii Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 23: Keeping Good Records

    LC system. However, if a leak occurs, correct it as soon as possible. Always wear eye and skin protection when operating or maintaining an LC system. Always turn off the pump before attempting any maintenance. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual xxiii...

  • Page 24: Contacting Us

    To suggest changes to the documentation or to the Help • Fill out a reader survey online at www.surveymonkey.com/s/PQM6P62 –or– Click the link below. • Send an e-mail message to the Technical Publications Editor at techpubs-lcms@thermofisher.com. xxiv Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 25: Introduction

    Note For information about earlier versions of the Accela pumps, see Appendix A, “Legacy Hardware Components.” Note In this manual, the term Accela pump refers to the Accela 600 Pump and the Accela 1250 Pump. For information about the Accela Pump, refer to the Accela Pump Hardware Manual.

  • Page 26: Functional Description

    50 to 2000 μL/min (isocratic) 1250 bar (18 130 psi) to 2000 μL/min (gradient) The gradient delay volume of the LDA is 90 μL. The gradient delay volume of the LDA is 70 μL. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 27: Hardware Components

    The integral vacuum degasser offers superb solvent degassing efficiency using a built-in vacuum membrane degasser that removes dissolved gasses from the mobile phase. The Accela 600 Pump and the Accela 1250 Pump are benchtop units that you can integrate with other Accela instruments.

  • Page 28: Vacuum Degasser Assembly

    2 ppm. By varying its speed, the vacuum pump maintains a constant vacuum level. This eliminates the excess baseline noise and drift caused by vacuum pump cycling. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 29: Liquid Displacement Assembly (Lda)

    35 μL of gradient delay volume to the system. You can plumb the system to bypass the dynamic mixer for mobile phases that do not require additional mixing. For information about installing the dynamic mixer, see “Dynamic Mixer Installation” page Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 30 In addition to the dynamic mixer and the seal wash pump, you can add an optional leak sensor that detects the presence of liquid on the pump’s drip tray. For information about installing the leak sensor, see “Leak Sensor Installation” page Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 31: Status Leds

    Flashing amber A failure, such as a loss of vacuum, has occurred. For additional diagnostics information, see “Degasser Troubleshooting Guide” page 118. Green Sufficient vacuum has developed to perform liquid chromatography. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 32: Specifications

    36.0 cm (14 in.) Depth 45.7 cm (18 in.) Height 18.0 cm (7.1 in.) Weight 19.5 kg (43 lb) Table 5. Accela 600 Pump operating specifications Specification Description Backpressure range 0 to 600 bar (8702 psi) Flow rate range 1 to 5000 μL/min Residual pulsation <...
  • Page 33 Teflon AF Table 8. Electronic specifications Specification Description Communications 19 200 baud, 1 start, 8 data, 1 stop, no parity and no handshaking, proprietary protocol/USB 1.0 Audio output Piezo buzzer Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 35: Accela 600 Pump Or Accela 1250 Pump Installation

    Accela 600 Pump or Accela 1250 Pump Installation To install an Accela pump as part of an Accela LC system, follow the “Accela Pump Installation Checklist” page 12 and the procedures in this chapter. For information about installing hardware options, see Chapter 3, “Hardware Upgrades.”...

  • Page 36: Accela Pump Installation Checklist

    Accela 600 Pump or Accela 1250 Pump Installation Accela Pump Installation Checklist Accela Pump Installation Checklist When the Accela pump (600 or 1250) arrives at your facility, a Thermo Fisher Scientific field service engineer performs the initial installation. When you move the pump from one location to another, follow the steps in this installation checklist to ensure that the Accela pump operates properly as part of an Accela LC system.

  • Page 37: Shipment Inspection

    Accela 600 Pump or Accela 1250 Pump Installation Shipment Inspection Shipment Inspection Carefully remove the Accela pump from the shipping container and inspect the pump and packing for any signs of damage. If you find any damage, save the shipping materials and immediately contact the shipping company.
  • Page 38 Accela 600 Pump or Accela 1250 Pump Installation Benchtop Setup The minimum benchtop width for a system that includes an additional LC pump or an additional detector is 94 cm (37 in.). The minimum benchtop width for a system that includes an additional LC pump and an additional detector is 1.5 m (5 ft).

  • Page 39: Layout For An Accela Lc System With An Accela Open Autosampler

    Accela 600 Pump or Accela 1250 Pump Installation Benchtop Setup Layout for an Accela LC System with an Accela Open Autosampler Figure 4 shows the recommended layout for a stand-alone Accela LC system with an Accela Open Autosampler. This setup requires a minimum benchtop area 68 cm (27 in.) wide by 72 cm (28 in.) deep.
  • Page 40 Accela 600 Pump or Accela 1250 Pump Installation Benchtop Setup Figure 4. Recommended layout for an Accela LC system with an Accela Open Autosampler Width 68 cm (27 in.) Accela Open Autosampler Height RS-232 connection 104.5 cm to the data system...

  • Page 41: Back Panel Connections

    Accela 600 Pump or Accela 1250 Pump Installation Back Panel Connections Back Panel Connections To operate the Accela pump from the data system computer, connect the power cable and the USB communication cable. To operate the Accela pump as part of an Accela LC system, connect the interconnect cable that coordinates the operation of the pump with the other Accela modules.

  • Page 42: Connecting The Power Cable

    Accela 600 Pump or Accela 1250 Pump Installation Back Panel Connections To make the back panel connections, follow these procedures: • Connecting the Power Cable • Connecting the Pump to the Data System Computer • Connecting the Accela System Interconnect Cables •...

  • Page 43: Connecting The Pump To The Data System Computer

    Accela 600 Pump or Accela 1250 Pump Installation Back Panel Connections Connecting the Pump to the Data System Computer The Accela pump communicates with the data system computer through a USB connection.  To connect the pump to the computer with a USB cable 1.
  • Page 44 1. Plug one of the pump connectors with a yellow PUMP sticker into the 8-pin socket on the back panel of the Accela pump (all models). Table 10 lists the pin out descriptions for the 8-pin socket. Table 10. Accela 600 Pump interconnect cable socket pin out descriptions Description Description Pump ready...
  • Page 45 Accela 600 Pump or Accela 1250 Pump Installation Back Panel Connections Figure 9. System interconnect cable connections for the Accela LC system Connector labeled Connector labeled DETECTOR DETECTOR 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16...
  • Page 46 Figure 10 shows the contact closure cable for an Accela LC system with an Accela Open Autosampler and one or two Accela 600 Pumps, Accela 1250 Pumps, or a combination of these pumps. Tip An unlabeled cable equivalent to the Accela Open Autosampler interconnect cable (P/N 60157-63024) ships with the autosampler.
  • Page 47 1. Connect the Accela Open Autosampler interconnect cable (P/N 60157-63024) to the Accela Open Autosampler and the Accela 600 or 1250 Pump as follows: a. Plug the DB15 connector into the Interface 1 receptacle on the back panel of the Accela Open Autosampler.
  • Page 48 Accela 600 Pump or Accela 1250 Pump Installation Back Panel Connections Figure 12. Contact closure connections for an Accela LC system with an Accela Open Autosampler Accela Open Autosampler INTERFACE 1 Connection between the Accela Open Autosampler interconnect cable and the detector adapter cable...

  • Page 49: Additional Hardware Configurations

    Accela 600 Pump or Accela 1250 Pump Installation Back Panel Connections Additional Hardware Configurations Use the D-sub 25-pin connector on the back panel of the Accela pump to connect the pump to an Accela Open Autosampler or a valve interface module.

  • Page 50: Solvent Line Connections

    High-Pressure Solvent Line Between the Pump and the Autosampler Low-Pressure Solvent Reservoir Lines The Accela 600 (and 1250) Pump Inlet Tubing Kit contains four preassembled solvent reservoir lines and the Accela System Kit contains four solvent reservoir bottles and four solvent reservoir bottle caps.
  • Page 51 Accela 600 Pump or Accela 1250 Pump Installation Solvent Line Connections d. Reconnect the 20 μm particle size, stainless steel filter to the end of the solvent line (see Figure 13). IMPORTANT Use only stainless steel, 20 μm particle size sinker frits to terminate the solvent reservoir lines.
  • Page 52 Solvent Line Connections Connecting the Solvent Reservoir Bottles to the Degassing Unit The Accela 600 Pump Inlet Tubing Kit, which ships with both the Accela 600 Pump and the Accela 1250 Pump, contains four preassembled solvent reservoir lines. Each solvent line terminates with a color-coded Super Flangeless fitting at one end and a stainless steel, 20 μm particle size sinker frit at the other end.
  • Page 53 Accela 600 Pump or Accela 1250 Pump Installation Solvent Line Connections Figure 15. Flat and angled sides of the steel compression ring Angled side Flat side  To connect the solvent lines to the degasser inlets of the Accela pump 1.

  • Page 54: High-Pressure Solvent Line Between The Pump And The Autosampler

    Accela 600 Pump or Accela 1250 Pump Installation Solvent Line Connections Priming the Degassing Membranes Prime the degassing membranes as part of the initial Accela pump installation procedure. CAUTION To avoid damaging the degassing unit and the piston seals, prime the degassing membranes by using a syringe to pull solvent through the solvent lines into the degassing unit.
  • Page 55 Accela 600 Pump or Accela 1250 Pump Installation Solvent Line Connections Connecting the High-Pressure Solvent Line to the Pump Outlet Use the supplied precut, stainless steel tubing (0.005 in. ID × 1/16 in. OD) to connect the pump to the autosampler: •...
  • Page 56 Accela 600 Pump or Accela 1250 Pump Installation Solvent Line Connections Figure 18 shows the two-piece, compression fitting. The length of the nut is 0.45 inches, and the length of the ferrule is 0.19 inches. Figure 18. Two-piece stainless steel fitting 1/16 in.
  • Page 57 Accela 600 Pump or Accela 1250 Pump Installation Solvent Line Connections CAUTION To prevent personal injury, before installing the LC column or connecting the pump to the autosampler, ensure that the column oven compartment is at room temperature. The column oven compartment can reach temperatures as high as 95 C (203 F).
  • Page 58 Accela 600 Pump or Accela 1250 Pump Installation Solvent Line Connections Figure 20. Connection between the Accela pump and the Accela Autosampler Heat exchanger inlet connection Access slot in the autosampler drip tray Dynamic mixer Connecting the Solvent Line to the Accela Open Autosampler Injection Valve The Accela Open Autosampler has a high-pressure Valco™...

  • Page 59: Powering On The Pump

    Accela 600 Pump or Accela 1250 Pump Installation Powering On the Pump Figure 21. Accela Open Autosampler injection valve and wash station Injection port of the injection valve Wash station High-pressure solvent line connection to the LC column High-pressure solvent line connection from the LC pump 3.

  • Page 61: Hardware Upgrades

    To install the optional dynamic mixer, seal wash pump, or leak sensor, follow the instructions in this chapter. Contents • Dynamic Mixer Installation • Seal Wash Pump Installation • Leak Sensor Installation • Setting Up Continuous Flushing Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 62: Dynamic Mixer Installation

    Connecting the Dynamic Mixer to the Pump’s Front Panel Connecting the Dynamic Mixer to the Controller Board Reconnecting the Pump Chassis to the Enclosure Reconnecting the Static Mixer and the High-Pressure Lines Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 63: Removing The Pump Chassis From The Enclosure

    (placeholder) module 3. If the high-pressure tubing is connected between the pump and the autosampler, using a 1/4 in. open-end wrench, disconnect the high-pressure solvent line from the blind outlet module. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 64 Figure 25. Front view of the pump with the doors open Screw that secures Four screws that secure the metal plate the enclosure to the chassis 5. Disconnect the LED cable from the left door of the pump (see Figure 26). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 65 If you are installing one of these other upgrade options, do the following: • To continue installing the seal wash pump, go to step 4 page • To continue installing the leak sensor, go to step 6 page Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 66: Connecting The Dynamic Mixer To The Pump's Front Panel

    Figure 27. Back side of the pump’s front panel showing the screws (with washers) being removed Screws that secure the blind outlet (placeholder module) to the front panel of the pump Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 67 3. Using a 1/4 in. open-end wrench, disconnect the static mixer from the LDA (see Figure 29 Figure 30). Figure 29. Static mixer connection to the LDA Connection between LDA and static mixer Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 68 4. Insert the dynamic mixer into the port vacated by the blind outlet (placeholder) module (see Figure 31). Figure 31. Dynamic mixer inserted into the port vacated by the blind outlet module Dynamic mixer Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 69 Figure 33. Dynamic mixer secured to the pump chassis Screws that secure the dynamic mixer to the pump Go to the next procedure, “Connecting the Dynamic Mixer to the Controller Board.” Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 70: Connecting The Dynamic Mixer To The Controller Board

    To complete the installation of the dynamic mixer, go to the next procedure, “Reconnecting the Static Mixer and the High-Pressure Lines.” Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 71: Reconnecting The Static Mixer And The High-Pressure Lines

    LDA outlet Static Dynamic mixer mixer inlet 2. Connect the outlet of the dynamic mixer to the autosampler inlet (see “High-Pressure Solvent Line Between the Pump and the Autosampler” page 30). Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 72: Seal Wash Pump Installation

    (see Figure 36). Set the screws aside. Note You use these screws in step c page 50 to secure the seal wash pump to the front panel of the pump. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 73 With the cable connector facing up (see Figure 37), slide the seal wash pump into the front panel of the pump (see Figure 38 page 50). Figure 37. Seal wash pump Cable connector Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 74 Seal wash pump c. Using the two hex-socket screws that you removed in step a page 48, secure the seal wash pump to the front panel of the pump (see Figure 39). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 75 Using a 2.5 mm hex ball driver, tighten the hex-socket screws. 3. Remove the pump chassis from the enclosure (see “Removing the Pump Chassis from the Enclosure” page 39). CAUTION Observe precautions for handling electrostatic sensitive devices. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 76 5. Reconnect the pump chassis to the enclosure (see “Reconnecting the Pump Chassis to the Enclosure” page 46). To set up the seal wash pump for continuous flushing, go to “Setting Up Continuous Flushing” page Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 77: Leak Sensor Installation

    Mix the two-component adhesive on a disposable surface, such as a sheet of weighing paper. c. Add a thin layer of adhesive to the back side of the mounting bracket. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 78 CAUTION To avoid damage to the Interface board and the Controller board, observe precautions for handling electrostatic sensitive devices. 5. Remove the pump chassis from the enclosure (see “Removing the Pump Chassis from the Enclosure” page 39). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 79 56, you replace this cable with a cable that connects to the J14, P18, and P8 connectors. Figure 43. Pump chassis with a view of the Interface board and the Controller board J14 connector P8 connector P18 connector Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 80 44) to the J14 connector on the Interface board and the P18 and P8 connectors on the Controller board. Figure 44. New cable (provided in the Leak Sensor Kit) J14 connector P18 connector Leak sensor connector P8 connector Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 81 9. Press the leak sensor latch against the mounting bracket until it clicks in place (see Figure 46). Figure 46. Leak sensor secured to the mounting bracket Leak sensor latch Leak sensor Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 82 10. Route the leak sensor cable through the slot below the mounting fixture for the LDA on the front panel of the pump (see Figure 47). Figure 47. Front panel of the pump with the LDA removed Slot for the leak sensor cable Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 83 12. Reconnect the pump chassis to the enclosure (see “Reconnecting the Pump Chassis to the Enclosure” page 46). 13. Reattach the LDA to the pump’s front panel (see “Reattaching the LDA to the Pump” page 92). Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 84 Green leak sensor LED Figure 50. Red LED indicates a wet drip tray Red leak sensor LED For information on specifying the configuration settings for the leak sensor, see Chapter 4, “Instrument Driver Configuration.” Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 85: Setting Up Continuous Flushing

    2. Remove the inner wash line from the guide bushings (see Figure 51). Figure 51. Guide bushings with the inner wash line removed In port of the seal wash pump Inner wash line removed Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 86 Figure 52 shows the solvent line connections for the seal wash pump. Figure 52. Seal wash pump solvent line connections Seal wash pump Connection to Seal wash pump inlet outlet waste Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 87: Chapter 4 Instrument Driver Configuration

    Specifying the Leak Sensor Settings in the Xcalibur Data System ChromQuest Instrument Configuration To control the Accela 600 Pump or the Accela 1250 Pump from the ChromQuest data system, add an Accela instrument to the ChromQuest Enterprise or add the pump to an existing Accela instrument.
  • Page 88 • Select the New Instrument icon, and then choose File > Configure > Instrument. –or– • Right-click the New Instrument icon, and then choose Configure > Instrument from the shortcut menu. The Instrument Configuration dialog box appears (see Figure 54). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 89 • To create a new instrument, click OK to close the message box. –or– • To modify the configuration of an existing instrument, click Cancel and do not make a selection in the Instrument Type list. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 90 If you have connected two pumps to the data system computer, the last five digits of the serial numbers for both pumps appear in the two Serial Number lists. The serial numbers are on the back panels of the pumps (see Figure 57). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 91 Model: 1250 Pump Serial No: 925000 The Status readback changes from Disconnected to Connected, and the Pump Type readback for Pump 1 (and Pump 2 for a dual-pump system) specifies Accela 600 or Accela 1250 (see Figure 58). In a dual-pump system, the two pumps can be different pump types.
  • Page 92 8. Click OK to accept the configuration and close the Accela Pump Configuration dialog box. 9. Click OK to close the Accela dialog box. 10. Click OK to close the Instrument Configuration dialog box. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 93: Xcalibur Instrument Configuration

    4. For Xcalibur versions earlier than 2.1, from the computer desktop, choose Start > All Programs > Xcalibur > Instrument Configuration. The Instrument Configuration window appears (see Figure 60). Figure 60. Instrument Configuration window (for Xcalibur version 2.0.7) Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 94 Ensure that pump 2 is connected to the data system computer. c. Turn on pump 2. d. Reopen the Instrument Configuration window as described in step 4 page e. Go to step 4 “Adding Pump 2 to the Instrument Configuration” page Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 95: Foundation Instrument Configuration

    You can control one or two Accela pumps from the data system computer. A dual-pump system can contain two Accela 600 Pumps, two Accela 1250 Pumps, or an Accela 600 Pump and an Accela 1250 Pump. The Accela 600 Pump and Accela 1250 Pump drivers can recognize and control either pump.
  • Page 96 Figure 61. Unloading Device Drivers status box Figure 62. Thermo Foundation Instrument Configuration dialog box 5. In the Available Devices area, double-click the Accela 600 or the Accela 1250 icon. Note Because you can install only one Accela pump driver, the Available Devices area contains either an Accela 600 or Accela 1250 icon.
  • Page 97 8. If your Accela pump has a leak sensor, select the Leak Sensor Installed check box. The leak sensor is an option for the Accela 600 Pump and the Accela 1250 Pump. The leak sensor has an optical sensor that turns red when covered by liquid.

  • Page 98: Adding Pump 2 To The Instrument Configuration

    7. If your Accela pump has a leak sensor, select the Leak Sensor Installed check box. 8. Click OK to accept the configuration settings and close the Accela pump type dialog box. 9. Click Done to close the Instrument Configuration application. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 99: Specifying The Leak Sensor Settings In The Xcalibur Data System

    3. In the view bar, click the icon that represents your Accela pump. The instrument setup view for your Accela pump appears. 4. From the menu bar, choose Accela 600 Pump or Accela 1250 Pump > Options. The Options dialog box appears (see Figure 65).
  • Page 100 2. Open the Information view in the Xcalibur data system. 3. Right-click the directory listing for the pump and choose Turn Device On from the shortcut menu. 4. Click OK to accept the selection and close the Options dialog box. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 101: Maintenance

    Cleaning the Instrument Casing • Flushing the LDA • Flushing the Piston Guide Bushings • Removing Air from the Piston Chambers • Cleaning the Displacement Chambers and the Inlet Module • Replacing the Fuses Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 102: Maintenance Schedule

    As needed Removing Air from the Piston As needed Chambers Cleaning the Displacement Chambers As needed and the Inlet Module Replacing the Fuses As needed Replacing the Secondary Piston Seals As needed Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 103: Accessing, Disassembling, And Reassembling The Lda

    4 mm hex ball driver To loosen the LDA attachment screws To loosen the fastening screws that secure the holding hooks to the LDA 2.5 mm hex ball driver To remove the Z-arm cover Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 104 Figure 66. Four proportioning valves plugged into the front panel Four plugs for the Four solvent lines connected proportioning valves to the inlet module Figure 67. Proportioning valve plugs unplugged from the front panel Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 105 5. Using a 1/4 in. open-end wrench, unscrew the static mixer at the outlet of the LDA and at the inlet of the placeholder module (see Figure 68). Figure 68. Static mixer connections to the LDA and the outlet module LDA outlet Static Placeholder module mixer inlet Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 106 4.0 cm screws 7. Using a 4 mm hex wrench, loosen the two clamping screws by three or four turns (see Figure 70). Figure 70. Loosening the two clamping screws Clamping screws Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 107 9. Gently push the piston heads to the left so that they do not contact the Z-arms (see Figure 72). Figure 72. Piston heads pushed to the left, away from the Z-arms Z-arm Piston head Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 108 Valve D (in front of the lower right attachment screw) 11. Gently pull the LDA off the two guide pins. Do not let the pistons touch the Z-arms (see Figure 74). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 109: Disassembling The Lda

    The cable is hardwired to the valve. As you disassemble the LDA, take care to avoid damaging the electrical wires inside the insulated cable. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 110 To disassemble the LDA 1. Remove the piston back flush tubes by gently pulling them off (see Figure 76). Figure 76. Location of the piston back flush tubes Piston back flush tubes Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 111 3. Carefully remove the pistons, guide bushings, and holding hooks from the LDA body (see Figure 78). Figure 78. LDA with the pistons, guide bushings, and holding hooks removed Holding hook Piston Guide bushing Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 112 5. Slide out the outlet module, check valves, and displacement chambers (see Figure 80). Figure 80. Sliding out the check valves, displacement chambers, and outlet module Check Check Outlet valve valve module Lower Upper displacement displacement Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 113: Reassembling The Lda

    LDA without the cables. Figure 82. Inserting the displacement chambers, check valves, and outlet module into the LDA Check Check Outlet valve valve module Lower Upper displacement displacement chamber chamber Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 114 Figure 83. Installing the compression lid Compression lid 8. Using the 4 mm hex wrench, firmly tighten the compression lid screw (see Figure 84). Figure 84. Tightening the compression lid screw Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 115 Repeat the same procedure for the second piston unit. Figure 85. Inserting the piston/guide bushing/holding hook assembly 11. Firmly tighten both holding hooks (see Figure 86). Figure 86. Tightening the holding hooks Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 116: Reattaching The Lda To The Pump

    Figure 87. LDA with the piston back flush tubes reinstalled Piston back flush tubes 3. Place the LDA onto the drive unit using the two alignment pins as a guide (see Figure 88). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 117 CAUTION When tightening the screw near the inlet valve, do not touch or apply force on inlet valve D or it might break. Figure 89. LDA attachment screws attachment screws Valve D (in front of the lower right attachment screw) Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 118 Figure 90. Proper orientation of the piston retaining springs Piston retaining springs 6. Using the 4 mm hex wrench, tighten the two clamping screws (see Figure 91). Figure 91. Tightening the two clamping screws Clamping screws Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 119 8. Carefully reconnect the static mixer (see Figure 93) to the LDA outlet and the placeholder module inlet. Figure 93. Static mixer connected to the LDA and the placeholder module LDA outlet Static Placeholder module mixer inlet Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 120 9. Reconnect the solvent lines to the inlet module. 10. Plug the proportioning valve cables into the receptacles on the front panel of the pump (see Figure 94). Figure 94. Plugging the proportioning valve cables into the receptacles Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 121: Replacing The Primary Piston Seals

    “Disassembling the LDA” page 85). 3. Remove the piston from the holding hook and guide bushing (see Figure 96). Figure 96. Piston, holding hook, and guide bushing assembly Piston Guide bushing Holding hook Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 122 6 mm (0.24 in.) (see Figure 98). Figure 98. Piston, guide bushing, and replacement piston seal assembly Guide bushing Piston seal 6 mm (0.24 in.) Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 123 Figure 99. Using the guide bushing and piston to insert the piston seal 11. Hold down the guide bushing and slide the piston up until it is out of the seal (see Figure 100). Figure 100. Sliding the piston out of the seal Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 124 14. Reassemble the LDA and reattach it to the pump (see “Reassembling the LDA” page 89). 15. Reattach the LDA to the pump (see “Reattaching the LDA to the Pump” page 92). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 125: Replacing The Secondary Piston Seals

    Thermo Fisher Scientific representative has instructed you to do so as part of a corrective or troubleshooting procedure. The secondary piston seals are made of polyethylene (PE) for the Accela 600 Pump and GFP for the Accela 1250 Pump.
  • Page 126 10. Reassemble the LDA (see “Reassembling the LDA” page 89). 11. Reattach the LDA to the pump (see “Reattaching the LDA to the Pump” page 92). Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 127: Cleaning The Check Valves

    Note If the pistons are scratched or appear cloudy, replace them. 4. Reassemble the LDA (see “Reassembling the LDA” page 89). 5. Reattach the LDA to the pump (see “Reattaching the LDA to the Pump” page 92). Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 128: Cleaning The Instrument Casing

    2. Pump LC/MS-grade methanol or IPA through the LDA for an additional 10 minutes.  To flush non-buffered acetonitrile mobile phases out of the LDA Pump LC/MS-grade methanol or IPA through the LDA for10 minutes. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 129: Flushing The Piston Guide Bushings

    1. Connect a 10 mL syringe filled with distilled water to the bottom-outer tubing (see Figure 105). Figure 105. Syringe connected to the guide bushings for flushing Purge valve wing nut 2. Gently push the water through the tubing. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 130: Removing Air From The Piston Chambers

    4. Close the purge valve, remove the syringe, and return to the initial conditions. CAUTION Wear safety gloves when handling solvents. 5. Check the pressure variation to determine if removing the trapped air stabilized the system pressure. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 131: Cleaning The Displacement Chambers And The Inlet Module

    “Removing the LDA from the Pump” page 79). • Reassemble the LDA and reattach it to the pump (see “Reassembling the LDA” page 89 “Reattaching the LDA to the Pump” page 92, respectively). Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 132: Replacing The Fuses

    5 × 20 mm, 2.0 A, 250 V, time-lag, UL, CSA 230 Vac 5 × 20 mm, 1.0 A, 250 V, time-lag, UL, VDE 4. Plug the pump power cord into the power outlet. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 133: Troubleshooting

    Run these tests if you want to validate the pump’s performance or if you suspect the pump is not functioning properly. • Testing the Flow Rate • Testing the Residual Pulsation • Testing the Pump Proportioning Accuracy Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 134: Testing The Flow Rate

    8. Compare the actual flow rate with the set flow rate. Under these conditions, the accuracy of the flow should be ±0.5%. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 135: Testing The Residual Pulsation

    9. Wait 5 minutes to allow the pump pressure to equilibrate. 10. Observe the pressure graph for pressure fluctuations. The pressure standard deviation should be less than 1 bar (14.5 psi). Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 136: Testing The Pump Proportioning Accuracy

    Wait an additional 2 minutes. 3. Pump 100% water and stabilize the baseline at 254 nm as follows: a. Set channel A to 100%. b. Set the UV detector to monitor 254 nm. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 137 30.1 Hold on channel C 34.0 Step E (34.0–34.1) 34.1 Hold (34.1–38.5) 38.5 Step F (38.5–38.6) 38.6 Hold (38.6–43.0) 43.0 Step G (43.0–43.1) 43.1 Hold (43.1–48.5) 48.5 Step H (48.5–48.6) 48.6 Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 138 6. Determine the height (absorbance value) of the steps (A through H) from baseline to top (see Figure 110). Figure 110. Step gradient profile for testing compositional accuracy (Accela pump validation software) Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 139 Pass criteria 25% Step Height: _______% 24.5–25.5% 50% Step Height: _______% 49.5–50.5% 75% Step Height: _______% 74.5–75.5% 9. If any value falls outside the range, call Thermo Fisher Scientific Technical Support. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 140: Error Messages

    Power Failure Error The drive motor has drawn excessive current. Solenoid Error A proportioning valve’s solenoid is disconnected or defective. Physical problem with the hardware or connectors: Contact an authorized dealer. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 141: Troubleshooting Guides

    “Cleaning the Check Valves” page 103. Pressure limit error Check the pressure limits. Pump remote start/stop Incorrect wiring Check the wiring. not working. Incorrect gradient table settings Check the method and the method options. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 142: Degasser Troubleshooting Guide

    Draw the solvent through the channel to dissolve the buffer. Do not push the solvent through the channel. If this flushing action does not work, contact your Thermo Fisher Scientific field service representative. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 143: Chapter 7 Replacement Parts

    • Accela 600 Pump Accessory Kit • Accela 1250 Pump Accessory Kit • Accela System Kit • Accela 600 Pump and Accela 1250 Pump Inlet Tubing Kit • Compression Fittings for High-Pressure Connections • Tools • Seal Wash Pump Kit •...

  • Page 144: Main Body

    Figure 111. Although some of the descriptions specify the Accela 600 Pump, all of the parts in Table 20 are compatible with both the Accela 600 Pump and the Accela 1250 Pump. Table 20. Main body assembly parts...
  • Page 145 Z-arm cover Screw M3 × 40 (14) (11) (10) (16) (15) (13) (12) Cable Cable Cable Inlet Cable Drip tray Front panel valve A valve B valve C Module valve D Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 146: Accela 600 Pump Lda

    Table 21 Figure 112. The item numbers in the table correspond to the numbers in the figure. The Accela 600 Pump has ceramic pistons. The primary and secondary piston seals ares made of GFP. Table 21. Accela 600 Pump LDA parts...
  • Page 147 Replacement Parts Accela 600 Pump LDA Figure 112. Accela 600 Pump LDA parts Outlet module UHP sealing ring, PEEK Upper displacement chamber Check valve, titanium Lower displacement chamber UHP LDA main body Piston unit (S-finish), ceramic Holding hook Secondary piston seal (PE)

  • Page 148: Accela 1250 Pump Lda

    Table 22. Accela 1250 Pump LDA parts Item Quantity Description Part number Outlet module, complete 00950-01-00284 (compatible with both the Accela 600 Pump and the Accela 1250 Pump) Ultra-high-pressure PEEK sealing ring 00950-01-10013 Displacement chamber, upper 00950-01-00123 Check valve, titanium and ruby...
  • Page 149 Lower displacement chamber UHP LDA main body Sapphire piston Holding hook (13) Secondary piston seal (GFP) Compression lid (11) including set (10) Primary piston screw Guide bushing seal (GFP) (12) LDA adapter Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

  • Page 150: Inlet Valve Assembly

    00950-01-00287 O-ring, 2.69 × 1.14 mm 00950-01-00286 Parts for valve fixation 00950-01-00288 Figure 114. Inlet valve assembly Proportioning Flow O-ring Parts for valve valve and cable brake 2.69 × 1.14 fixation Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 151: Service Spares

    Service Spares Table 24 lists services spares common to the Accela 600 Pump and the Accela 1250 Pump that you can order from Thermo Fisher Scientific. Table 24. Service spares that are common to both pumps (Sheet 1 of 2)
  • Page 152 Valve, proportioning, Accela 600 Pump 00950-01-00285 Valve, check 00110-05110 Table 25 lists the services spares for the Accela 600 Pump that you can order from Thermo Fisher Scientific. Table 25. Accela 600 Pump service spares Description Part number Bushing, guide, Accela 600 Pump...

  • Page 153: Accela 600 Pump Accessory Kit

    Replacement Parts Accela 600 Pump Accessory Kit Accela 600 Pump Accessory Kit Table 27 lists the parts provided in the Accela 600 Pump Accessory Kit. Table 27. Accela 600 Pump Accessory Kit (P/N 60157-62006) Quantity Description Part number Luer adapter Fuse, 1.0 A, 250 V, 5 ×...

  • Page 154: Accela 1250 Pump Accessory Kit

    00302-99-00014 Wrench, L-hex, 4 mm, RoHS Tool, ball driver, 2.5 mm Seal, high-pressure, GFP, Accela Pump 00950-01-00129 Tool, fitting extender, Accela 600 Pump 00725-01-00023 Kit, Inlet Tubing, Accela 600 Pump 60157-62008 Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 155: Accela System Kit

    Marker letter D, clip on for 1/8 in. OD tubing 608250004 Tubing, stainless steel, 0.005 in. ID × 1/16 in. OD, 00301-01-00009 20 cm length (connects the Accela Pump outlet to the inlet of the Accela Autosampler heat exchanger) Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 156 Tubing, stainless steel, 0.005 in. ID × 1/16 in. OD, 00028-01-00035 26 cm length (connects the Accela 600 Pump or the Accela 1250 Pump outlet to inlet of the Accela Autosampler heat exchanger) Tubing, stainless steel, 0.005 in. ID × 1/16 in. OD,...

  • Page 157: Accela 600 Pump And Accela 1250 Pump Inlet Tubing Kit

    Accela 600 Pump and Accela 1250 Pump Inlet Tubing Kit The Accela 600 (and 1250) Pump Inlet Tubing Kit (P/N 60157-62008) contains four preassembled solvent reservoir lines. Use this kit for both the Accela 600 Pump and the Accela 1250 Pump.

  • Page 158: Seal Wash Pump Kit

    Seal Wash Pump Kit. Figure 115. Tubing, fittings, solvent filter, and solvent bottle in the Seal Wash Pump Kit Figure 116. Seal wash pump in the Seal Wash Pump Kit Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 159: Dynamic Mixer

    The part number for the optional dynamic mixer (see Figure 118) is 00950-01-00298. Tip To secure the dynamic mixer to the Accela 600 Pump, use the screws that secure the blind outlet (placeholder) module. Figure 118. Dynamic mixer Outlet port...

  • Page 161: Appendix A Legacy Hardware Components

    This appendix describes legacy hardware components for the Accela pumps. Contents • Proportioning Valves • Fittings and Tubing • Replacement Parts for the Legacy Proportioning Valves Proportioning Valves Figure 119 shows the original proportioning valves for the Accela pumps. Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 162 Legacy Hardware Components Proportioning Valves Figure 119. Front panel of the Accela 600 Pump, showing the original proportioning valves Liquid displacement assembly Vacuum degasser Static (Optional) dynamic mixer or assembly mixer outlet adapter Purge valve (Optional) seal wash pump or cover plate ...

  • Page 163: Fittings And Tubing

    High-Pressure Fittings Low-Pressure Solvent Lines The original Inlet Tubing Kit for the Accela 600 and Accela 1250 pumps (P/N 60157-62008) contained four solvent reservoir lines. Each solvent line consisted of a 3 m (10 ft) length of 1/16 in. ID × 1/8 in. OD, FEP tubing with a 1/4 in. × 28 PEEK flangeless fitting at one end and a 20 μm particle size, stainless steel filter at the other end.
  • Page 164 3. For each solvent reservoir line, connect the flangeless fitting to the appropriate inlet port of the built-in degassing unit. Figure 123 shows the flangeless fitting connected to the solvent reservoir line. The flangeless fittings come in four colors: blue, green, yellow, and orange. Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 165: High-Pressure Fittings

    To replace a legacy proportioning valve, use the following part numbers. Description Part number Proportioning valve cable 00950-01-00289 Proportioning valve 00950-01-00285 Figure 124 shows the proportioning valve cable. Figure 125 shows the proportioning valve (item 4). Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 166 Legacy Hardware Components Replacement Parts for the Legacy Proportioning Valves Figure 124. Proportioning valve cable Figure 125. Inlet valve assembly Flow O-ring Parts for valve Proportioning brake 2.69 × 1.14 mm fixation valve Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...

  • Page 167: Index

    D-sub 25-pin connector checklist, installation dual-pump system contact closure, Accela Open Autosampler ChromQuest data system, instrument configuration 22–24 Thermo Foundation Instrument Configuration Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...
  • Page 168 Accela 600 and 1250 Pump Hardware Manual Thermo Scientific...
  • Page 169 (legacy) solvent requirements xxiii Thermo Scientific Accela 600 and 1250 Pump Hardware Manual...

This manual is also suitable for:

Accela 1250

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