Acceleration Rate And Deceleration Rate - Thermo Scientific Sorvall MTX 150 Instruction Manual

2

Acceleration rate and deceleration rate

In order to meet various experimental protocols, the acceleration and deceleration rates can be
adjusted.
The figure and table below show the relationship between ACCEL/DECEL code numbers selected and
resulting approximate acceleration/deceleration times.
Note The times for acceleration and deceleration may be longer than the values listed above,
depending upon the type of rotor in use.
Typical examples of application of acceleration and deceleration rates
Type of centrifugation
Density gradient centrifugation
using a vertical rotor
DNA separation by CsCl isopycnic
separation (self-forming gradients)
Pelleting using a fixed angle rotor
Density gradient centrifugation
using a swinging bucket rotor
2-14 Sorvall MTX 150
1 - 9 : ACCEL/DECEL code numbers
1 - 9 : ACCEL/DECEL code numbers
Code No.
ACCEL DECEL
5 7
9 7
9 9
5 to 8 5 to 8
Code
No.
9
8
7
(No Brake)
6
5
4
3
2
1
F
*
rotor is being accelerated or decelerated with the
Time
maximum torque of the driving motor. This time varies
with the type of rotor in use.
Characteristic of separation
The sample and gradient in tubes reorient during acceleration
and deceleration. Therefore, the sample and gradient can
become mixed, especially in wide tubes, if you use rapid
acceleration or deceleration.
You can operate at maximum acceleration because the density
gradient is not formed during the run. As for the deceleration, it
is better to decelerate slowly to obtain sharp bands.
Rapid pelleting of samples is possible (the run time decreases).
The sample and gradient do not reorient. Therefore, mixing of the
layers is less than that in the case of using a vertical rotor. But it
is safe not to accelerate or decelerate the rotor by selecting
minimum time.
Acceleration time
(minutes)
from rest to 5000 rpm from 5000 rpm to rest
Minimum time*
0.5
1
1.5
2
2.5
3
3.5
4
-
The minimum time is the one that occurs when the
Deceleration time
(minutes)
Minimum time*
1
2
3
4
5
6
7
8
Coasting
deceleration
Thermo Scientific