Thermo Scientific MicroCL 17 Instructions For Use Manual
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Thermo Scientific MicroCL 17 Instructions For Use Manual

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IEC
M
CL 17/ 17R 21/21R
ICRO
Instructions for use

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Summary of Contents for Thermo Scientific MicroCL 17

  • Page 1 CL 17/ 17R 21/21R ICRO Instructions for use...
  • Page 2 How to use this manual Use this manual to get acquainted with your centrifuge and its accessories. Overleaf you will find a graphic illustra- tion of the control panel with a survey of The manual helps you to avoid inappropriate handling. the most important functions Make sure to keep it always close to the centrifuge.
  • Page 3 (only for instruments with refrigeration unit) temperature speed/RCF run time quick run rotor turns open lid stop start rpm/RCF “set“ keys “set“ keys pre-temperature-regulation switch key (only for instruments with refrigeration unit) display Before switching on the centrifuge Note that “min ”...
  • Page 4 Control panel Keys Display panels start: start of the centrifuge Speed / RCF stop: manually stop of a run Resting state: current value (0) or pre-selected set-point value open lid: open lid During run: present current value of speed or RCF (possible only with the instrument switched on and (after activation of switch key) standstill of the rotor)

  • Page 5: Table Of Contents

    Contents Contents For your safety..........3 Handling the rotor ........21 Safety instructions in this manual ......3 Rotor lid with a spring lock ......... 21 Proper use..............4 Operation without rotor lid ........22 Improper use ............4 Rotor lid with screw plug ........23 Centrifuging hazardous materials ......
  • Page 6 Contents Pretemp .............. 36 Troubleshooting ........47 Starting the centrifuge ..........37 Mechanical emergency lid release......47 Changing the settings during the run ..... 37 Problems you can handle yourself ......49 Stopping the centrifuge .......... 38 Contacting Service ..........55 Preset run time ...........

  • Page 7: For Your Safety

    For your safety IEC centrifuges are manufactured according to current For your safety technical standards and regulations. Nonetheless, centrifuges may pose danger to individuals and sur- Safety instructions in this manual rounding if • they are not used as designed This symbol denotes potential hazards •...

  • Page 8: Proper Use

    For your safety being centrifuged, aerosol-tight bio-seals have to be Proper use used. The centrifuge is designed to separate liquid- For materials in a higher risk group, more than one suspended materials having different densities and precaution is required. particle size (maximum sample density is 1.2 g/cm³ at •...

  • Page 9: Conformity To Current Standards

    For your safety • Never open the lid manually if the rotor is still turn- Conformity to current standards ing. IEC centrifuges are manufactured and tested accord- • You may use the emergency lid release only in ing to the following standards and regulations case of emergency, e.g.
  • Page 10 For your safety for your notes...

  • Page 11: Description Of The Instrument

    Description of the instrument Pieces delivered Description of the instrument • rotor The descriptions apply generally for all instrument • a special wrench for securing the rotor variants. • cable for mains connection • this Manual Safety systems CL 17/21 CL 17R/21R ICRO ICRO...

  • Page 12: Properties

    Description of the instrument Properties If you press one of the keys repeatedly, you increase or decrease the corresponding pre-selected set point value stepwise. If you press the chosen key The M CL 17/21 and M CL 17R/21R are table- ICRO ICRO and hold it pressed, the respective chosen measure-...

  • Page 13: Before Use

    Before use Before use Transport and installation the centrifuge Where to install the centrifuge The centrifuge may only be used indoors. Its location Transport the centrifuge only in must meet the following criteria: the upright position, using the • A safety zone of 30 cm around the centrifuge must special box provided with the in- be maintained.

  • Page 14: Mains Connection

    Before use Mains connection Removing the transport protection Make sure that the mains supply and the frequency Before using the centrifuge, make sure meet the specifications printed on the centrifuge type that the rotor transport protection has plate. been removed! First turn the mains switch of the centrifuge off (press Turn the instrument on at the power switch.

  • Page 15: Accessories

    Accessories Accessories You may choose from among a large variety of rotors available as accessories. In addition, there are sets of adapters and reduction sleeves for diverse commercially available vessels. Please consult our sales documentation for a complete collection of accessories including technical data and order numbers.

  • Page 16: Rotors For The Iec Mirco Cl 17

    Accessories Rotors for the IEC M CL 17 IRCO Table 1: Performance characteristics for IEC M CL 17 ICRO rotor designation Micro liter-rotor Micro liter-rotor Dual-rotor 24 x 2 ml 36 x 0,5 ml 18 x 2 ml / 0,5 ml order no.
  • Page 17 Accessories Table 1: Performance characteristics for IEC M CL 17 ICRO rotor designation PCR-rotor PCR-rotor Haematocrit-rotor 4 x 8 8 x 8 order no. 75003440 75003489 75003473 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0,5)

  • Page 18: Rotors For The Iec Micro Cl 21

    Accessories Rotors for the IEC M CL 21 ICRO Table 2: Performance characteristics for IEC M CL 21 ICRO rotor designation Micro liter-rotor Micro liter-rotor Dual-rotor 24 x 2 ml 36 x 0,5 ml 18 x 2 ml / 0,5 ml order no.
  • Page 19 Accessories Tabelle 2: Performance characteristics for IEC M CL 21 ICRO rotor designation PCR-rotor PCR-rotor Haematocrit-rotor 4 x 8 8 x 8 order no. 75003440 75003489 75003473 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0,5)

  • Page 20: Rotors For The Iec Micro Cl 17R

    Accessories Rotors for the IEC M CL 17R ICRO Table 3: Performance characteristics for IEC M CL 17R ICRO rotor designation Micro liter-rotor Micro liter-rotor Dual-rotor 24 x 2 ml 36 x 0,5 ml 18 x 2 ml / 0,5 ml order no.
  • Page 21 Accessories Table 3: Performance characteristics for IEC M CL 17R ICRO rotor designation PCR-rotor PCR-rotor Haematocrit-rotor 4 x 8 8 x 8 order no. 75003440 75003489 75003473 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0,5)

  • Page 22: Rotors For The Iec Micro Cl 21R

    Accessories Rotors for the IEC M CL 21R ICRO Table 4: Performance characteristics for IEC M CL 21R ICRO rotor designation Micro liter-rotor Micro liter-rotor Dual-rotor 24 x 2 ml 36 x 0,5 ml 18 x 2 ml / 0,5 ml order no.
  • Page 23 Accessories Table 4: Performance characteristics for IEC M CL 21R ICRO rotor designation PCR-rotor PCR-rotor Haematocrit-rotor 4 x 8 8 x 8 order no. 75003440 75003489 75003473 places / volume 4 x PCR-Strip 8 x PCR-Strip 24 x Blood capillary tubes 75 mm maximum permissible load [ g ] 4 x 4 (32 x 0,5)

  • Page 24: Adapter

    Accessories Adapter Table 5: Adapter max. tube tube capacity number color no. of Adapter for dimensions [ ml ] per set order Micro Liter Rotor 7500 3424 x length Dual-rotor 7500 3418 [ mm ] reduction sleeve PCR 6.2 x 20 grey 7600 3750 reduction sleeve...

  • Page 25: Handling The Rotor

    Handling the rotor Rotor lid with a spring lock Handling the rotor Opening Rotor temperature The rotor lid is held on the central rotor nut integrated The rotors are only to be used within into the rotor the temperature range from - 9°C to +40°C.

  • Page 26: Operation Without Rotor Lid

    Handling the rotor - Closing Operation without rotor lid For closing the rotor place the rotor lid centrically on If you are going to operate the rotor without the rotor lid the rotor nut. the aerosol-seals must be removed prior to use. Press the rotor lid down now until the lock is audio- visually locked Aerosol-seals...

  • Page 27: Rotor Lid With Screw Plug

    Handling the rotor Rotor lid with screw plug For this kind of rotor the rotor lid will be held centrically These rotors are not designed for on the rotor collar. aerosoltight applications! The O-Ring in the rotor collar pro- vides only for tight fit of screw plug. The external lid lip can not be sealed for these rotors.

  • Page 28: Aerosol-Tight Application

    Handling the rotor Tubes are only to be filled to a level where the sample Aerosol-tight application does not reach the brim of the tubes during centrifuga- Only with the designated rotors! tion. See the rotor tables from page 12 Please observe the permissible filling volumes! Nominal volume: Permissible volume:...
  • Page 29 Handling the rotor Correct operation when filling the sample tubes and The following steps have to be carried out: closing the rotor lid are prerequisites for aerosol bio- • Lubricate the seals before inserting them containment. (lubricant order no. 76003500) Prior to each application, the seals in the rotors have to be checked for correct fit, abrasion or damage and...

  • Page 30: Checking For Aerosol-Tightness

    Handling the rotor Checking for aerosol-tightness For a quick test one can check the fixed angle rotors The checking of the rotor type and bucket was done according to the following procedure: according to the dynamic microbiological test proce- • Slightly grease all seals.

  • Page 31: Operation

    Operation Operation Opening the lid Switching on the centrifuge For activating the lid release the centrifuge Set the main power switch of the instrument. must be connected to mains and switched For a couple of seconds the following reading appears in the control panel: (the temperature display field only- for cooled instrument) To open the lid press the „open-lid“-button...

  • Page 32: Installing The Rotor

    Operation Installing the rotor Proceed as follows: 1. Open the centrifuge lid and make sure that the Improper or improperly combined rotor chamber and the rotor are clean. Remove accessories may cause severe dam- eventual dust, debris or sample residues. The age to the centrifuge! thread and the O-Ring on the motor shaft must be in perfect condition.
  • Page 33 Operation 4. Press the rotor gently down until it stops. 5. Grip the rotor tightly and use the provided rotor wrench to tighten up the rotor. Do not push the rotor down using force. If the rotor can not be tightened, you have to remove it carefully, align the notch and the retaining pin again and re-install it.

  • Page 34: Loading The Rotor

    Operation Filling the centrifuge tubes Loading the rotor Maximum load Please note that plastic sample vessels only have a limited service life - particularly when Overloading may cause the rotor used at maximum performance (rpm or tem- to explode! perature) - and must be replaced as neces- sary! Exploding parts may severely damage the centrifuge!

  • Page 35: Placing The Tubes In The Rotor

    Operation Placing the tubes in the rotor Uneven loading may lead to damage of The rotor must be loaded symmetrically. When loading the rotor and centrifuge in extreme the rotor only partially, you must ensure that opposite cases. Imbalance not only causes a bores always receive tubes of equal weight (when cen- noisy run, but also causes premature trifuging a single sample, place a centrifuge tube e.g.

  • Page 36: Entering Parameters

    Operation Entering parameters Switching from speed to RCF display By pressing the key briefly, you increase or decrease Upon turning on the centrifuge, the speed display is the speed in one step (of set. 100 rpm). By activating the selection key you can switch to the This option is supposed to RCF- value or change between rpm and RCF displays.

  • Page 37: Entering The Rcf Value

    Operation Concerning the RCF value Entering the RCF value The relative centrifugal force (RCF) is given in multi- You can adjust the RCF pre-selected value in steps of ples of the earth gravity g. It is a dimensionless number 100 g. The setting of pre-selected value is entered value that allows one to compare the efficiency of analogously to the speed.

  • Page 38: Selecting The Run Time

    Operation Selecting the run time 3. Release the key as soon as you have reached the You can select a run time between 1 and 99 min or desired value and fine tune if necessary by repeat- continuous operation [hd]. edly briefly pressing one of the keys.

  • Page 39: Setting The Temperature

    Operation Setting the temperature 3. Release the key as soon as you are close to the To determine the sample temperature for instruments desired value and fine tune if necessary by repeat- with refrigeration unit, operate as adviced: edly briefly pressing the key. The display flashes for 1.

  • Page 40: Pretemp

    Operation Pretemp If you wish to change the temperature of your samples, The pretemp-function allows quick and easy tempering please consider that the time required for temperature of the unloaded rotor. adjustment is prolonged. The farther apart initial and After calling the function by pressing the key only final temperature, the longer it takes for the tempera- the desired temperature still must be chosed.

  • Page 41: Starting The Centrifuge

    Operation Changing the settings during the run Starting the centrifuge You can change the settings while the rotor is spinning. Once the rotor is properly installed, the main switch Trough a one-time pressing of any key of the control turned on and the lid closed, you can start the centri- panel, the current value will switch into the pre- fuge.

  • Page 42: Stopping The Centrifuge

    Operation Stopping the centrifuge Short-time centrifugation Preset run time For short-term operation, the centrifuge is equipped with the "quick run" function. Normally the run time has been pre-selected, and all you have to do, is to wait until the centrifuge terminates Short-term centrifugation is started by pressing the the run automatically.

  • Page 43: Removing The Rotor

    Operation Removing the rotor Audible alarm To remove the rotor you have to proceed in the reserve Accompanying all error messages, a warning signal is order as at the installation of the rotor. given out which can be silenced upon pressing any key.

  • Page 44: Turning Off The Centrifuge

    Operation Turning off the centrifuge WEEE Compliance: The centrifuge is turned off by switching the main This product is required to comply with the European switch into the "0"position. Union`s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: The main power switch should be turned off after a complete centrifugation run.

  • Page 45: Maintenance And Care

    Maintenance and care Cleaning Maintenance and care Maintenance to be performed by the Pull mains plug before cleaning the customer instrument! For the protection of persons, environment and mate- rial you are obliged to clean the centrifuge regularly Clean the casing, the rotor chamber, the rotor and the and to disinfect it if necessary.

  • Page 46: Cleaning The Filter Unit

    Maintenance and care Please clean the venting slots regularly! To protect the refrigeration unit, the cooled devices are equipped with an air filter unit. Depending environment situation Sucking grid recommended to clean the filter at least every three months. Disconnect the centrifuge from mains switch before cleaning the venting slots and the filter unit.

  • Page 47: Disinfection

    Maintenance and care Disinfection If a centrifuge tube containing infectious material Rotor and rotor chamber must be treated with a neu- leaks, during a centrifugation run, you have to disinfect tral, universal desinfectant. Best suited for this purpose immediately the rotor, and/or also the centrifuge. are disinfectant sprays, ensuring that all rotor and ac- cessory surfaces are covered evenly.
  • Page 48 Maintenance and care 5. Treat the rotor and the rotor lid according to the Disinfection with bleaching lye instructions given for the disinfectant (soaking in liquid or spraying). You must strictly observe the These agents contain highly ag- specified action times! gressive hypochlorites and must 6.

  • Page 49: Decontamination

    Maintenance and care Decontamination The rotor must be cleaned and rinsed with distilled For general radioactive decontamination, use a solu- water before being autoclaved. Remove the rotor lid, tion of equal parts of 70% ethanol, 10% SDS and wa- the centrifuge tubes and the adapters. Place plastic ter.

  • Page 50: The Thermo Electron Service Offer

    Maintenance and care The Thermo Electron Service offer Warranty conditions Thermo Electron recommends annual servicing of the The warranty period starts with the day of delivery. centrifuge and the accessories by authorized customer Within the warranty period the centrifuge is repaired or service or trained professionals.

  • Page 51: Troubleshooting

    Troubleshooting Troubleshooting Mechanical emergency lid release 1. Make sure the rotor stands still. (control window). In case of a power failure you cannot open the lid nor- mally using the electric emergency release. To permit 2. Unplug the mains cord. unloading even in this case, the centrifuge is equipped 3.
  • Page 52 Troubleshooting 4. Gently press the centrifuge lid down to disengage the lid-latch. 5. While pressing down the lid push the wire down further until you hear and feel the lid-latch unlock- ing. Remove the auxiliary tool and open the centri- fuge lid.

  • Page 53: Problems You Can Handle Yourself

    Troubleshooting Problems you can handle yourself If problems other than those described in the following tables arise, you must consult your nearest authorized service. Error Symptom Possible causes and corrective measures Displays remain dark The drive stops. Mains voltage disconnection The rotor stops without 1.
  • Page 54 Troubleshooting Error Symptom Possible causes and corrective measures – Exceptionally running Imbalance. noise. 1. Stop the centrifuge by pressing the "stop" key, in case of emergency, unplug mains power cord. 2. Wait until the centrifuge comes to a complete stop. 3.
  • Page 55 Troubleshooting Error Symptom Possible causes and corrective measures „bAL“ The rotor stops with Imbalance switch releases. deceleration. 1. Open the instrument by pressing the “lid open” in case. 2. Check whether the rotor is properly loaded. 3. Check whether a broken tube or a damage to the rotor release the imbalance switch.
  • Page 56 Troubleshooting Error Symptom Possible causes and corrective measures E-22/E-23 Rotor stops without Error in speed entry deceleration to standstill. Switch the instrument off and on again. Instrument cannot be If the error persists, contact service representative. operated. E-24 Instrument cannot be Wrong status information from the lid-latch.
  • Page 57 Troubleshooting Error Symptom Possible causes and corrective measures E-31 Rotor stops without Overtemperature in the motor. deceleration to standstill or 1. Turn instrument off and unplug mains power cord. does not start. 2. Check and clean the venting slots if necessary and respectively the filter unit of the cooled centrifuge.
  • Page 58 Troubleshooting Error Symptom Possible causes and corrective measures E-41 Rotor stops without Internal program error. deceleration to standstill. Switch the instrument off and on again. Instrument cannot be E-56 If the error persists, contact service representative. operated. E-60 Rotor stops with decelera- Insufficient temperature in the refrigeration unit.

  • Page 59: Contacting Service

    Troubleshooting Contacting Service Subsequently, the following readings will be dis- played for 5 seconds each Should you require help from your service represen- SoFt 0 5 8 tative, please indicate the catalog and serial number Software number of your instrument. You will find the pertinent infor- _ 0 1 Software version mation at the specifications, near the socket for the...
  • Page 60 Troubleshooting for your notes...

  • Page 61: Technical Data

    Technical data Technical data Component parts Part / function Description Hausing/body Sheet steel chassis with attached plastic housing and steel reinforcement Keys and display panel Keys and display panel covered with easy care protective foil Operation "Easycontrol" system Rotor chamber dimensions (D x H) (diameter x height): 190 mm x 70 mm IEC M...

  • Page 62: The "Easycontrol" User Interface

    Technical data The "Easycontrol" user interface Function Performance Start key ( Start Stop key ( Stop Quick starting and stopping "Quick run" key ( ): short-time run when pressed permanently; stop when released Indication of operating state Spinning rotor indicated by rotating lights (LED) in the speed display panel End of centrifugation Speed display reads "End"...

  • Page 63: Performance

    Technical data Performance Performance Value/description CL 17 / IEC M CL 17R in parentheses) (IEC M ICRO ICRO environmental conditions indoor use maximum elevation 2000 m above sea level maximum relative humidity 80 % up to 31 °C; linearly decreasing down to 50 % relative humidity at 40 °C. permissible temperature of the environment 5 °C to + 40 °C during operation (no condensation) -10 °C to 50 °C for storage and shipping...
  • Page 64 Technical data Performance Value/description dimensions (H x W x D) IEC M CL 17/21 230 mm x 240 mm x 350 mm ICRO IEC M CL 17R/21R 330 mm x 292 mm x 440 mm ICRO weight with rotor IEC M CL 17/21 10,5 kg ICRO...

  • Page 65: Electrical Connections

    100 V 50/60 Hz 2,9 A 170 W 2 x 6,3 AT 250V (6,3 x 32 mm) IEC MicroCL 17, Haematocrit 7500 2495 230 V 50/60 Hz 1,4 A 180 W 2 x 4,0 AT 250V (5 x 20 mm)
  • Page 66 Technical data for your notes...

  • Page 67: Appendix

    Appendix Appendix...
  • Page 68 Appendix...

  • Page 69: Speed / Rcf Diagram

    Appendix Speed / RCF diagram Speed/RCF diagram Micro liter rotor 24 x 2 ml 7500 3424 100000 = 14 800 min = 8,6 cm RCF (r ) = 21 058 = 5,1 cm 10000 1000 1000 10000 100000 speed (rpm)
  • Page 70 Appendix Speed/RCF diagram Micro liter rotor 36 x 0,5 ml 75003436 100000 = 14 800 min = 7,9 cm RCF (r ) = 19 344 = 5,0 cm 10000 1000 1000 10000 100000 speed (min...
  • Page 71 Appendix Speed/RCF diagram Dual rotor 18 x 2 ml / 0,5 ml 75003418 100000 = 14 800 min = 8,5 cm RCF (r ) = 20 813 = 4,8 cm 10000 1000 1000 10000 100000 Speed (min...
  • Page 72 Appendix Speed/RCF diagram PCR-rotor 4 x 8 75003440 100000 = 14 800 min = 6,6 cm RCF (r ) = 16 161 = 4,7 cm 10000 1000 1000 10000 100000 speed (min...
  • Page 73 Appendix Speed /RCF diagram PCR-rotor 8 x 8 75003489 100000 = 14 800 min = 7,0 cm RCF (r ) = 17 140 = 4,4 cm 10000 1000 1000 10000 100000 speed (min...
  • Page 74 Appendix For your notes...

  • Page 75: Autoclaving Protocol

    Appendix Autoclaving protocol Date Remark Operator Signature...
  • Page 76 Appendix Autoclaving protocol Date Remark Operator Signature...

  • Page 77: Index

    Index Index corrosion 4 corrosive substances protective tubes for corrosive substances 4 acceleration 8 acceleration time 12, 13, 14 aerosol-tight 12, 13, 14, 26 aerosol-tightness damage test 26 symbol for potential 3 aluminum rotor: 44 dangerous chemicals 4 approved rotors 28 deceleration time 12, 13, 14 autoclaving 45 decontamination 43, 45...
  • Page 78 Index E-01 ... E-60 51 "quick run" 8 "start" 37 fine tuning keys temperature 35 general operation 8 formula kinetic energy 59 maximum permissible load 30 fuses 61 blockage 49 handling the centrifuge 4 Lid lock with safety check 7 Handling the rotor 21 light hazardous substances 4...
  • Page 79 Index mechanical lid release 47 pretemp 36 problems handling of 49 protective tubes for corrosive substances 4 noise 59 pulse function 38 noise suppression g 60 pulse key 8 operation quick run function 38 continuous 38 quick run key 8 preselected run time 34 short-time 38 Order no.
  • Page 80 Index partial loading 31 short-time operation 38 rotor cap 29 site of installation 9 rotor insertion software check temperature 28 internal 27 rotor wrench 39 software version rotors determination 55 approved 28 speed 32 run time permissible 30 continuous operation 34 speed, maximum 12, 13, 14, 59 range 34 start key 37...
  • Page 81 Index tube breakage with infectious material 43 Turning the centrifuge off 40 warning signal 39 warranty conditions 46 wear 4 weight 60 wrench unbalance detection 31 for rotor insertion 28...
  • Page 82 Index...
  • Page 83 D-63505 Langenselbold Phone: +49 (0) 61 84 / 90 60 00 Fax: +49 (0) 61 84 / 90 70 00 20058331 IEC MicroCL 17/21-R UK 10/2006 Printed in Germany...

This manual is also suitable for:

Microcl 17rMicrocl 21Microcl 21r

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