How To Use The Option Function - Thermo Scientific Sorvall MTX 150 Instruction Manual

Type of centrifugation
Density gradient
centrifugation using a
vertical rotor
DNA separation by CsCl
isopycnic separation
(self-forming gradients)
Pelleting using a fixed
angle rotor
Density gradient
centrifugation using a
swinging bucket rotor

How to use the option function

How to use the option function
This micro-ultracentrifuge incorporates a number of features, such as step-mode and other
programmed running, display and setting of centrifugal force, and RTC (real-time control) that can
run the micro-ultracentrifuge at a required date and time. Buttons for these features are displayed and
specified on the Run screen.
SPEED/RCF button
Figure 2-6. Option buttons
Thermo Scientific
Code No.
Characteristic of separation
ACCEL DECEL
5 7 The sample and gradient in tubes reorient during acceleration
and deceleration. Therefore, the sample and gradient can
become mixed, especially in wide tubes, if you use rapid
acceleration or deceleration.
9 7 You can operate at maximum acceleration because the density
gradient is not formed during the run. As for the deceleration,
it is better to decelerate slowly to obtain sharp bands.
9 9 Rapid pelleting of samples is possible (the run time
decreases).
5 to 8 5 to 8 The sample and gradient do not reorient. Therefore, mixing of
the layers is less than that in the case of using a vertical rotor.
But it is safe not to accelerate or decelerate the rotor by
selecting minimum time.
2
RTC button
PROGRAM button
Sorvall MTX 150 2-15