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Summary of Contents for Thermo Scientific mDrop
- Page 1 Thermo Scientific μDrop and μDrop Duo Plates User Manual Cat. No. N12691 Rev 4.0 2020...
- Page 2 Copyright Copyright © 2020 Thermo Fisher Scientific Inc. All rights reserved. Reproduction of the accompanying user documentation in whole or in part is prohibited Manufacturer Life Technologies Holdings Pte. Ltd. (a part of Thermo Fisher Scientific Inc.) 33, Marsiling Industrial Estate Road 3, #7-06, Singapore 739256 Trademarks “μDrop”...
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Page 3: Preface
Preface About This Guide This manual gives instructions on performing absorbance measurements using the Thermo Scientific™ μDrop™ and μDrop™ Duo Plates with the following instruments: • Thermo Scientific™ Multiskan™ SkyHigh • Thermo Scientific™ Multiskan™ Sky • Thermo Scientific™ Multiskan™ GO •... -
Page 4: Contacting Us
About This Guide Contacting Us For the latest information on products and services, visit our website at: www.thermofisher.com/platereaders Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific... -
Page 5: Table Of Contents
General specifications ..........23 Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual... - Page 6 Contents Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific...
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Page 7: Introduction To The Μdrop And Μdrop Duo Plates
Introduction to the μDrop and μDrop Duo Plates Introduction Thermo Scientific™ μDrop™ and μDrop™ Duo Plates are tools designed for quick and easy absorbance measurement of low sample volumes (2–10 μl) and cuvettes with the following instruments: • Thermo Scientific™ Multiskan™ SkyHigh •... -
Page 8: General Description
On the right-hand side, the μDrop and μDrop Duo Plates have a slot for cuvette measurement (see Figure 1 Figure Figure 1. A closed μDrop Plate showing the low-volume measurement area (1), adjustment screw (2), and the cuvette measurement point (3). Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific... - Page 9 (3), low-volume area (4), and cuvette slot (5). Figure 3. A closed μDrop Duo Plate showing the low-volume measurement area (1), adjustment screw (2), and cuvette measurement point (3). Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual...
- Page 10 The measurement sessions for Multiskan SkyHigh, Multiskan Sky, Multiskan GO and Varioskan LUX can be created using Thermo Scientific™ SkanIt™ Software. It is also possible to measure the μDrop and μDrop Duo Plates with Multiskan SkyHigh and Multiskan Sky internal software ready-made protocols.
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Page 11: Low-Volume Measurements With Skanit Software
Figure 5. SkanIt Cloud library For more details about the SkanIt Cloud Library, refer to the Thermo Scientific™ SkanIt™ Software for Microplate Readers Technical Manual (Cat. No. N16046). It is recommended to visit the SkanIt Cloud Library to get the latest versions of the sessions, and also any possible new sessions. -
Page 12: User-Made Sessions
Software procedure. However, there are certain special features to observe: 1. Plate Layout Use the Thermo Scientific μDrop Plate or Thermo Scientific μDrop Duo Plate plate template in SkanIt Software. Select the correct template from the Change plate template dropdown menu, see Figure Thermo Scientific μDrop and μDrop Duo Plates User Manual... - Page 13 Selecting the plate template All existing sample types are available for the layout (see Figure 7 Figure 8) and calculations. Figure 7. Example layout of μDrop Plate containing only blanks Thermo Fisher Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual...
- Page 14 Note The μDrop and μDrop Duo Plates support only photometric measurements. Note The μDrop and μDrop Duo Plates do not support dispensing. Note Shaking the μDrop and μDrop Duo Plates is not recommended. Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Fisher Scientific...
- Page 15 Figure 10). Figure 10. Custom Formula For more details about SkanIt Software, refer to the Thermo Scientific™ SkanIt™ Software for Microplate Readers Technical Manual (Cat. no. N16046). Blanks Each measurement should contain a blank subtraction. It is recommended to use at least one blank sample in each measurement.
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Page 16: Placing The Samples
1. Pipette at least 2 μl of distilled deionized laboratory water into each well. Check that there are no air bubbles in the sample. 2. Measure the absorbance in each well with the daily routine measurement wavelength. Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Fisher Scientific... - Page 17 Plate thoroughly. For further instructions, refer to “Cleaning the μDrop and μDrop Duo Plates” page A spectral measurement is also a suitable tool to verify, for example, traces of DNA in the sample positions. Thermo Fisher Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual...
- Page 18 Low-volume measurements with SkanIt Software Cleaning Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Fisher Scientific...
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Page 19: Cuvette Measurements With Skanit Software
Placing the cuvette The cuvette is placed into the cuvette slot with the stopper pointing towards the adjustment screw and the reading window facing upwards (see Figure 12). Thermo Fisher Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual... -
Page 20: Creating A New Measurement Session
Software procedure. However, there are certain special features to observe: 1. Plate Layout Use the Thermo Scientific μDrop Plate or Thermo Scientific μDrop Duo Plate plate template in SkanIt Software. Select the correct template from the Change plate template dropdown menu. - Page 21 All existing sample types are available for the layout and calculations. Figure 13. Cuvette layout of μDrop Plate with a blank sample cuvette, Figure 14. Cuvette layout of μDrop Plate with an unknown sample Thermo Fisher Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual...
- Page 22 3. Results All available calculation methods may be used as with normal microplates. For more details about SkanIt Software, refer to the Thermo Scientific™ SkanIt™ Software for Microplate Readers Technical Manual (Cat. No. N16046). Thermo Scientific μDrop and μDrop Duo Plates User Manual...
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Page 23: Setting Up A Measurement From The Instrument
Multiskan Sky and Multiskan SkyHigh can be operated using the touch screen display of the instrument. For more details, refer to the Thermo Scientific™ Multiskan™ Sky Technical Manual (Cat. No. N18965) and Thermo Scientific™ Multiskan™ SkyHigh Technical Manual (Cat. No. N21872). - Page 24 3. Select the μDrop Plate or μDrop Duo Plate plate template. 4. Define the measurement parameters. 5. Tap Start to start the measurement. 6. View the results of the measurement. 7. Export the results if desired. Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific...
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Page 25: Chapter 5 Maintenance
5. If necessary, clean the other parts of the μDrop and μDrop Duo Plates with a soft cloth dampened with distilled deionized laboratory water or a mild detergent. 6. Perform cleaning verification. For more information, refer to “Cleaning verification” page Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual... -
Page 26: Troubleshooting
If you have spilled infectious agents, follow decontamination procedures. WARNING Decontamination procedures should be performed by authorized trained personnel wearing disposable gloves, protective glasses and clothing in a well-ventilated room. Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific... - Page 27 6. Close the bag firmly and leave the μDrop or μDrop Duo Plate in the bag for at least 24 hours. 7. Remove the μDrop and μDrop Duo Plates from the bag. 8. After decontamination, clean the μDrop or μDrop Duo Plate using a mild detergent. Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual...
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Page 28: Disposal Of The Μdrop And Μdrop Duo Plates
Follow laboratory and country-specific procedures for biohazardous or radioactive waste disposal. Use the recycling operators familiar to you for the original packaging and packing materials. For more information, contact your local Thermo Fisher Scientific representative. Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific... -
Page 29: Chapter 6 Technical Specifications
Cuvette function Cuvette with stopper: Dimensions: 12.5 (W) x 12.5 (D) x 47–54 (H) mm Beam center height: 8.5 mm Beam window: ≥ 4 mm Wavelength range 200–1000 nm Thermo Scientific Thermo Scientific μDrop and μDrop Duo Plates User Manual... - Page 30 Technical specifications General specifications Thermo Scientific μDrop and μDrop Duo Plates User Manual Thermo Scientific...
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