Create A Results Table Using The Quantitation Wizard - AB Sciex QTRAP 4500 LC/MS/MS system System User's Manual

ii. In the Internal Standard column, from the list, select the internal standard to
be associated with each analyte.
iii. In the Q1/Q3 column, select 609.400/195.039.
iv. If required, add one or more of the other compounds for a more complex
analysis.
Note: If the Compound ID field was populated for the samples and
internal standards in the acquisition method, then in the Analytes table, the
Name field and Q1/Q3 field are populated.
7. Click the Integration tab.
In general, the preset integration parameters are suitable for most peaks.
8. If the integration is not suitable, then change the algorithm.
9. Click the Show or Hide Parameters icon to show the additional integration
algorithms.
10. Click the Calibration tab. The preset parameters are suitable for these samples.
11. Save the quantitation method.
The new method can be used when a batch is created in the Batch Editor or when
the Quantitation Wizard is used to generate a Results Table.
Note: The quantitation method can only be used in the current project
unless it is copied to another project. To do this, click Tools > Project >
Copy Data. A new project must be created and selected to be available for
use.

Create a Results Table using the Quantitation Wizard

Prerequisite Procedures
• Select the Example project. Refer to
• Use the Analyst Classic algorithm. Refer to
It is recommended that the user validate any queries that are used to analyze data in a Results
Table.
1. In the navigation bar, under Quantitate, double-click Quantitation Wizard.
The Create Quantitation Set - Select Samples page opens.
2. In the Available Data Files list, double-click the Triple Quad folder.
3. Select Mix_Batch_2.wiff.
4. Click Add All Files.
5. Click Next.
The Create Quantitation Set - Select Settings & Query page opens.
6. In the Default Query section, click Select Existing: Query.
7. In the Query list, select Accuracy 15%.
System User Guide
RUO-IDV-05-0264-A
Operating Instructions — Analyze and Process Quantitative Data
Switch Between Projects and Subprojects on page
Show Basic Quantitative Data on page
47.
81.
4500 Series of Instruments
97 of 138