Disassembly And Cleaning Of Flow Cell - PerkinElmer 200A Series User Manual

Series 200a Fl User's Guide
138.
Fig. 6-7 Injection by Syringe
6. Replace the washing solution with the eluent that is used for separation, and repeat the
procedure in step (5).
7. If air bubbles are observed in the flow cell, supply solution into the cell with the pump at a flow
rate within 1.0 mL/min and a pressure within 1.0 MPa. While the solution is being supplied into
the flow cell, pinch the end of the drain tube with your fingers for a few seconds to increase
the pressure inside the cell, then release your fingers quickly. Repeat this procedure a few
more times until all the air bubbles have been removed.
Fig. 6-8 Removing Air Bubbles
Applying too much pressure may damage the flow cell. Set the pressure limiter of the pump at 1.0
NOTE:
MPa max. before removing air bubbles.

Disassembly and Cleaning of Flow Cell

1. Disassembly of Flow Cell
If contamination is not removed by just flushing the interior of the flow cell, then disassemble
and clean the cell in the procedure below.