PerkinElmer LAMBDA 365 User Manual
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PerkinElmer LAMBDA 365 User Manual

Uv spectrometers
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UV SPECTROMETERS
L
365
AMBDA
Users Guide

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Summary of Contents for PerkinElmer LAMBDA 365

  • Page 1 UV SPECTROMETERS AMBDA Users Guide...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    Operating Procedure ............... 53 Procedure .................... 54 Accessories ..................55 Accessories for the Lambda 365 ............. 56 Single Cell Holder ................56 Water Jacketed Single Cell Holder............ 57 8 Position Multicell Holder / Water Jacketd 8-Position Multicell Holder . 57 Micro Cell Holder ................
  • Page 4 4 . Lambda 365 Users Guide...

  • Page 5: Introduction

    Introduction . 5 I ntroduction...

  • Page 6: Overview

    6 . Lambda 365 Users Guide Overview This manual provides instructions for installing and operating the Lambda 365 Double Beam UV-Visible Spectrophotometer and UV Express software. For more detailed information on software features including Quantification, kinetics and bio software, refer to the UV Express...

  • Page 7: About This Manual

    Information on installing and re-installing your instrument should you ever need to move your system is provided. Chapter 4 UV Ex press Softw are I nstallation The complete UV Express software installation procedures for the Lambda 365 is in this chapter. Chapter 5 I nterface Setup Interface procedures for the Lambda 365.

  • Page 8: Basic Theory

    8 . Lambda 365 Users Guide Basic Theory Electromagnetic radiation can pass through most organic and inorganic compounds. Generally, the UV range is from 190 to 380 nm and visible range is from 380-800 nm. Figure 1. Electromagnetic Radiation When continuous radiation passes through a sample, some is absorbed by the sample. An absorption spectrum can be obtained by monitoring the radiation that penetrates the sample and reaches a detector.
  • Page 9 Introduction . 9 Figure 2. Energy Absorption Once a sample absorbs light energy, the intensity of incoming light P (the energy from the light source) reduces to P by losing some of its energy; therefore, we define the transmittance of a sample as follows: Figure 3.

  • Page 10: Double Beam Uv-Visible Spectrophotometer

    Figure 4. Transmittance and Absorbance Double Beam UV-Visible Spectrophotom eter Lambda 365 double-beam UV-Visible spectrophotometer, the light is split into two beams before it reaches the sample. One beam is used as the reference; the other beam passes through the sample. The reference beam intensity is taken as 100% Transmission (or 0 Absorbance), and the measurement displayed is the ratio of the two beam intensities.

  • Page 11: Safety Summary

    Introduction . 11 Safety Sum m ary The following safety symbols are used on this product. Warning Description Always refer to the system manual when working near locations at which the alert mark shown on the left is attached. If the operation, etc., is performed without heeding the advice in the System manual, there is a risk of personal injury.
  • Page 12 Warning NE PAS tenter d'ouvrir le boîtier ou de démonter ses pièces internes. Seul un représentant du service PerkinElmer qui est conscient des risques d'incendie et d’électrocution devrait réparer cet équipement. Cet équipement contient des parties à haute tension présentant des risques de blessure grave ou d’électrocution au personnel non qualifié.
  • Page 13 Introduction . 13 Warning Description For continued protection against risk of fire, replace only with same type and rating of fuse. Before changing the fuses, ALWAYS remove the power cord from the power-outlet and replace the blown fuses. Changing Fuse 250V~ T3.15AL indicates a time-lag fuse.

  • Page 14: Instrument Shipping Kit

    14 . Lambda 365 Users Guide I nstrum ent Shipping K it The following table shows what is included in the Lambda 365 Spectrophotometer shipping kit. Item Name Qty. Main Lambda 365 Double Beam UV-Vis Instrument Spectrophotometer Accessories Power Cord (US/EU/UK)

  • Page 15: Conventions Used In This Manual

    UPPERCASE text, for example ENTER or ALT, refers to keys on the PC keyboard. '+' is used to show that you have to press two keys at the same time, for example, ALT+F. All eight digit numbers are PerkinElmer part numbers unless stated otherwise. N otes, cautions and w arnings Three terms, in the following standard formats, are also used to highlight special circumstances and warnings.
  • Page 16 16 . Lambda 365 Users Guide We use the term CAUTION to inform you about situations that CAUTION could result in serious dam age to the instrum ent or other equipment. Details about these circumstances are in a box like this one.
  • Page 17 Introduction . 17 We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons. Details about these circumstances are in a box like this one. WARNING W arning (W arnung) Bedeutet, daß...

  • Page 18: Contact Us

    If you are located within the U.S., call toll free 1-800-762-4002, 8 a.m. to 8 p.m. EST. Your order will be shipped promptly, usually within 24 hours. If you are located outside of the U.S., call your local PerkinElmer sales or service office.

  • Page 19: Safety Practices

    Safety Practices . 19 Safety P ractices...

  • Page 20: Overview

    This advice is intended to supplement, not supersede, the normal safety codes in the user's country. It is also a supplement to the PerkinElmer standard Safety and Health Policy. The information provided does not cover every safety procedure that should be practiced.

  • Page 21: Precautions

    à prox im ité. The following precautions must be observed when using the Lambda 365: Be sure that the voltage of the Lambda 365 corresponds to the voltage used in your • laboratory.

  • Page 22: General Operating Conditions

    If any adjustment, maintenance and repair of the opened instrument is necessary, this must be done by a skilled person who is aware of the hazard involved. Whenever it is likely that the Lambda 365 is unsafe, make it inoperative. The Lambda 365 may be unsafe if it: shows visible damage •...

  • Page 23: Electrical Safety

    Safety Practices . 23 Electrical Safety The instrument has been designed to protect the operator from potential electrical hazards. This section describes some recommended electrical safety practices. Lethal voltages are present at certain areas w ithin the instrum ent. I nstallation and internal m aintenance of the instrum ent should only be perform ed by a P erk inElm er service engineer or sim ilarly authorized and trained person.
  • Page 24 24 . Lambda 365 Users Guide Do not attempt to make internal adjustments or replacements except as directed in the • manuals. • Disconnect the instrument from all voltage sources before opening it for any adjustment, replacement, maintenance, or repair. If afterwards, the opened instrument must be operated for further adjustment, maintenance, or repair, this must only be done by a PerkinElmer Service engineer.

  • Page 25: Emc Compliance

    EM C Com pliance EC directive The Lambda 365 has been designed and tested to meet the requirements of the EC directive 2006/95/EC. The Lambda 365 complies with the EMC standard EN61326-1, (EMC standard for electrical equipment for measurement, control and laboratory use) and EN61000-3.

  • Page 26: Warning Labels

    WARNING Lorsque cette étiquette est attachée à un instrument, il est nécessaire de voir le manuel. See the following figure for the location of warning labels on the Lambda 365. Warning labels on the back of the instrument: Warning Warning...

  • Page 27: Weee Instructions For Perkinelmer Products

    For Customer Care telephone numbers select “Contact us” on the web page. Products from other manufacturers may also form a part of your PerkinElmer system. These other producers are directly responsible for the collection and processing of their own waste products under the terms of the WEEE Directive.

  • Page 28: Decontamination

    762-4000 or (+1) 203-925-4602, 8:30 a.m. - 7 p.m. EST and speak to Customer Support. In Canada, call toll free 800-561-4646 and speak to Customer Support. If you are located outside of the United States or Canada, please call your local PerkinElmer sales office for more information.

  • Page 29: Fuses

    Safety Practices . 29 Fuses There is risk of receiving a fatal electric shock if the fuses are replaced with the power cord connected. WARNING Il y a un risque d’électrocution si les fusibles sont remplacés tandis que le cordon d'alimentation est encore branché. The fuse is located at the rear of the instrument.
  • Page 30 30 . Lambda 365 Users Guide...

  • Page 31: Lambda 365 Installation

    Lambda 365 Installation . 31 Lam bda 365 I nstallation...

  • Page 32: Preparing The Laboratory

    Pow er A grounded AC power supply must be used. The Lambda 365 accepts 100 to 240 VAC at 50 Hz or 60 Hz. I n order to protect the system from electrical noise and current surges, please connect the pow er cable to a GROUN DED OUTLET.
  • Page 33 Lambda 365 Installation . 33 Environm ental R equirem ents This system is for indoor use only. Requirement Description Indoor use Altitude up to 2000 m (6,500 ft.) Pollution degree 2 491 mm (W) × 538 mm (D) × 258 mm (H) Dimensions [19.3 in.

  • Page 34: Installation

    34 . Lambda 365 Users Guide I nstallation Carefully remove the instrument and all of its components from the shipping container. Verify that all of the components received match the packing list. Verify that all system components required for installation are present, this, must include: ...

  • Page 35: Uv Express Software Installation

    UV Express Software Installation . 35 UV Ex press Softw are I nstallation...

  • Page 36: How To Install The Uv Express Software

    36 . Lambda 365 Users Guide How to I nstall the UV Ex press Softw are The Sleep Mode in the Power Saver is not recommended to us while running the UV Express NOTE: software. Set the Power Saver as follows: a.
  • Page 37 Interface Setup . 37 ® UV Express InstallShield Wizard starts. Select Next.
  • Page 38 38 . Lambda 365 Users Guide Enter customer information, if necessary, and select Next. The software will be installed in C:\UV Express as a destination folder. To install the software to a different folder, select Change and select the desired folder.
  • Page 39 Interface Setup . 39 Verify the current settings and select Install. The following window shows the status of the UV Express installation.
  • Page 40 40 . Lambda 365 Users Guide After the setup is completed successfully, select Finish. The UV Express software icon will appear on the Desktop.

  • Page 41: Interface Setup

    Interface Setup . 41 I nterface Setup...
  • Page 42 42 . Lambda 365 Users Guide USB I nterface Setup 1. Connect the USB cable between the computer and the instrument. 2. Turn on the computer and the instrument. 3. Click Computer → CD Drive → Open. 4. Select USB Driver folder.
  • Page 43 Interface Setup. 43 5. Double click USBCom-CDM_20828. 6. Select Extract.
  • Page 44 44 . Lambda 365 Users Guide 7. Select Next. 8. The following dialog box will appear. After installation is completed successfully, select Finish.
  • Page 45 Interface Setup. 45 9. Change the USB serial Ports after finishing driver setup. See the following chapter for that procedure.
  • Page 46 46 . Lambda 365 Users Guide...

  • Page 47: Setting Usb Serial Ports

    Setting USB Serial Ports. 47 Setting USB Serial Ports...

  • Page 48: Procedure

    48 . Lambda 365 Users Guide Procedure 1. Select Computer →Properties. 2. Click on Device Manager.
  • Page 49 Setting USB Serial Ports. 49 3. Click on the Ports (COM & LPT) to expand the listing. These are the devices currently connected to the COM ports. USB Serial Port (COMx) is visible when the driver installation is completed successfully. 4.
  • Page 50 50 . Lambda 365 Users Guide 5. Select on the Port Settings tab and click on the Advanced… button. 6. Change the parameter values as shown below.
  • Page 51 7. Verify the parameters and click OK. 8. Launch the UV Express software. 9. If the Lambda 365 fails to communicate with the PC, change the COM Port Number as following procedure. 10. Open Advanced Setting for COMx window following on page 48~50.
  • Page 52 52 . Lambda 365 Users Guide 12. Make sure that the changed COM Port Number is applied, and select OK. 13. Restart the computer after finishing driver setting.

  • Page 53: Operating Procedure

    Operating Procedure . 53 Operating P rocedure...

  • Page 54: Procedure

    54 . Lambda 365 Users Guide Procedure 1. Turn on the power switch and allow the instrument to warm-up for at least 20 minutes. * Check the LED light, which is the lower left hand in front of the system.

  • Page 55: Accessories

    Accessories . 55 Accessories...

  • Page 56: Accessories For The Lambda 365

    If you are located within the U.S., call toll free 1-800-762-4002, 8 a.m. to 8 p.m. EST. Your order will be shipped promptly, usually within 24 hours. If you are located outside of the U.S., call your local PerkinElmer sales or service office. For more detailed information about the accessories, please refer to the instruction sheet for...

  • Page 57: Water Jacketed Single Cell Holder

    Accessories . 57 W ater Jack eted Single Cell Holder Use this accessory to control the temperature of a sample in a standard 10 mm rectangular cuvette. This accessory needs a circulator for control temperature with a separately purchased circulator. Optional stirring is available with a separately purchased stir controller. 8 P osition M ulticell Holder / W ater Jack etd 8-P osition M ulticell Holder Use this accessory to measure eight samples automatically.

  • Page 58: Variable Pathlength Cuvette Holder

    58 . Lambda 365 Users Guide Variable P athlength Cuvette Holder Use this accessory to hold rectangular cells with 5, 10, 20, 40, 50, 100 mm pathlengths. A moveable block secures cells in position. Advanced Transm ission Holder Use this accessory to measure the transmittance spectrum of various solid samples including films, glass, filters, and optical components.

  • Page 59: Variable Angle Transmission Holder

    Accessories . 59 Variable Angle Transm ission Holder Use for measuring the transmittance of various features of Film, Glass, and Plate, etc according to the variable angle. 50 m m Transm ission / R eflectance Sphere Used for measuring the reflectance or total transmittance of solid samples such as; metals, glass, textiles, paper, gems, and powders.

  • Page 60: Single Cell Peltier Holder, Controller

    60 . Lambda 365 Users Guide Single Cell P eltier Holder, Controller Control the temperature of a single 10 mm pathlength rectangular cell between -5 and 100℃. Peltier control offers fast ramping with recirculating liquid for exceptional temperature control. Integrated purging capability can be used to decrease condensation. This accessory includes a temperature probe, temperature controller, Peltier accessory and all accessories necessary for its operation and connection to the instrument.

  • Page 61: Nano Stick

    Accessories . 61 with heated reference without heated reference Peltier Controller N ano Stick Use this accessory to measure micro-volume sample such as DNA, RNA and Protein. Autosam pler Automate the measurement of up to 360 samples at a time with an autosampler accessory. Two and four rack configurations are available.
  • Page 62 62 . Lambda 365 Users Guide...

  • Page 63: Lamp Replacement

    Accessories . 63 Lam p Replacem ent...

  • Page 64: D2 Lamp Replacement

    64 . Lambda 365 Users Guide D2 Lam p R eplacem ent Make sure that the main power is turned off. If the system and lamp(s) were turned on at least 20 minutes should pass before the lamp changing process is started. This time allows the lamps to cool and return to room temperature.
  • Page 65 Accessories . 65 Loosen the two screws using a Phillips screwdriver. Note that there is no need to take the screws out from the lamp module. Carefully lift up the D2 lamp module and disconnect the D2 lamp cable connector. Replace the D2 lamp module with a new one and reassemble the parts in reverse order.

  • Page 66: Tungsten Lamp Module Replacement

    66 . Lambda 365 Users Guide Tungsten Lam p M odule Replacem ent Make sure that the main power is turned off. If the system and lamp(s) were turned on at least 20 minutes should pass before the lamp changing process is started. This time allows the lamps to cool and return to room temperature.
  • Page 67 Accessories . 67 Remove the two M3*6 screws on the sub cover using a Phillips screwdriver. Put the tungsten lamp changing tool on the tungsten lamp module carefully by aligning the notches over the lamp module securing screws.
  • Page 68 68 . Lambda 365 Users Guide Fasten the cap screw on the tungsten lamp changing tool to fix the tool to the tungsten lamp module. Using a Phillips screwdriver to loosen the two screws that are located on the tungsten lamp module.
  • Page 69 Accessories . 69 Carefully lift up the tungsten lamp changing tool which holds the tungsten lamp module and disconnect the tungsten lamp cable connector. Loosen the cap screw on the tungsten lamp changing tool (Part No. N4102048) and separate the tungsten lamp from the tool. Replace the tungsten lamp with a new one and reassemble the parts in reverse order.

  • Page 70: Index

    70 . Lambda 365 Users Guide I ndex About This Manual ..........7 Laboratory Preparation ......... 32 Accessories ............56 Lamp Replacement D2 Lamp ............64 Tunsten Lamp ..........66 Cleaning ............... 28 Cleaning the Instrument ........28 Computer Requirements ........33 Operating Conditions ..........

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