Check And Calibration Of Wavelength Accuracy - PerkinElmer 200A Series User Manual

Series 200a Fl User's Guide
130.
Fig. 6-5 Drift Measurement
Carry out the drift measurement after the xenon lamp has stabilized (at least one hour after the
NOTE:
lamp is turned on).

Check and Calibration of Wavelength Accuracy

1. Check of Wavelength Accuracy
a. Principle of measurement
Wavelength accuracy of the emission side monochromator is checked by using the bright
line at 254 nm of the built in Hg lamp. As for the wavelength accuracy of the excitation
side monochromator, the Em side is fixed at the wavelength position where the
abovementioned bright line was detected, then the Ex wavelength where a Rayleigh
scattering peak of 254 nm is produced is measured.
b. Check of wavelength accuracy
Check the wavelength accuracy by using the bright line at 254 nm of the Hg lamp (see
section 4.4.4 (6)). "GOOD" will be indicated if the wavelength error is within (3 nm, or
"FAIL" will be indicated if the error exceeds (3 nm.
NOTES: