Leakage From The Flow Cell - PerkinElmer 200A Series User Manual

Fluorescence detector
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Series 200a Fl User's Guide
140.
NOTE: Make sure to put the flow cell in a paper or plastic container when cleaning it. The use of a glass
container may scratch the cell or preclude obtaining the desired performance from the instrument.
3. Assembly of Flow Cell
a. Assemble the flow cell into the cell holder in the orientation shown in Fig. 6-11 and fix it
with the cell clamp.
b. Tighten the upper and lower retaining screws 1 and 2.
Fig. 6-11 Assembly of Flow Cell Unit
c. Attach the cell window and shielding plate.
d. After assembling the flow cell in the order reverse to disassembly, flow a liquid through the unit
and make sure there is no leakage.
Be careful not to scratch the flow cell surface when assembling it.
NOTE:
Take care about the orientation of the polished surfaces of the flow cell when putting it into the cell
NOTE:
holder (see Fig. 6-11). A mistake in the orientation may preclude obtaining the proper performance
from the instrument.
Tighten the setscrew 1 fully with hand, and then tighten the setscrew 2 about 30° manually or
NOTE:
tighten the setscrew 1 further 30° with a wrench.

Leakage from the Flow Cell

It is important to check for any liquid leakage from the flow cell unit prior to analysis. If a leak is
found, stop the pump immediately and remove the flow cell unit. Take the following procedure to
remedy the leak.

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