Operating Instructions; To Adjust The Auto Zero; Setting The Wavelength - PerkinElmer 200A Series User Manual

Fluorescence detector
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Series 200a Fi User's Guide
76.

Operating Instructions

Make sure the power switch of the detector is ON, and the display presents the 1st monitor screen.
Then the unit should be allowed to warm up at least 1 hour for stabilization of the xenon lamp
and accuracy of measurement.
Default value or currently set value is indicated before making entries.

To Adjust the Auto Zero

The output value should be zeroed when zero point adjustment is required or when the data baseline
deviates from the recorder zero point.
1. Press the A/Z key.
2. The FL indication on the 1st monitor screen is reset to zero and output signal becomes zero.
<The 1st monitor screen>
Setting value range: 0 to 1000
If the range is exceeded, an error message is displayed.

Setting the Wavelength

Setting items:
Ex (Excitation wavelength)
Em (Emission wavelength)
1. Press the WL key.
2. The following display will be presented. Excitation wavelength (Ex) is settable (cursor appears under
Ex).
<Ex wavelength setting screen>
Press numeric keys (to input 2 0 0 to 8 5 0) and then ENT key.
This wavelength is settable within a range from 200 to 850 nm in increments of 1 nm.
Default value: 250
For canceling the input excitation wavelength value, press the ESC key or
monitor screen returns.
TIME
Ex
Em
0.0
250
350
Ex
Em
(200 - 850) nm
250
350
FL
PROG
LAMP
0.0
1
Xe
[I]
key. The 1st

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