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Summary of Contents for PerkinElmer IVIS Lumina S5
- Page 1 H A R D W A R E M A N U A L IVIS Lumina S5 ® January 2018 PN CLS149599...
- Page 2 PerkinElmer reserves the right to revise this publication and to make changes from time to time in the content hereof without obligation of PerkinElmer to notify any person of such...
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Page 3: Table Of Contents
Location for the IVIS Lumina S5 ........ - Page 4 Basic Operation ......... . 18 Starting the IVIS Lumina S5 Imaging System ......18 Electrical Connections and Gas Plumbing .
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Page 5: Chapter 1 Welcome
Welcome Introduction Contact Information on page 2 1.1 Introduction The IVIS Lumina S5 is a high-sensitivity, low-noise imaging system enabling both fluorescent and bioluminescent imaging in vivo (Figure 1.1). The imaging system is compatible with the Smart High Throughput Imaging and ID Kit (CLS148875) and the MVI-2 Imager for multi-view imaging. -
Page 6: Contact Information
® IVIS Lumina S5 Hardware Manual Chapter 1 | Welcome 1.2 Contact Information If you have questions regarding this manual or the IVIS Lumina S5, please contact PerkinElmer Technical Support. PerkinElmer Health Sciences 940 Winter Street Waltham, Massachusetts 02451 USA www.PerkinElmer.com... -
Page 7: Chapter 2 Important Safety Instructions
Observance of safety precautions will help avoid actions that could damage or adversely affect the performance of the IVIS Lumina S5. If the equipment is used in a manner not specified in this manual, the protection provided by the equipment may be impaired. -
Page 8: Heed Warnings
2.4 Environmental Considerations for the System Components Location for the IVIS Lumina S5 Before the IVIS Lumina S5 is installed, consider the proper environment for the components. Install the equipment in an environment where: The temperature does not fluctuate widely and is maintained between 15-25 °C (59-77 °F). -
Page 9: Water And Moisture
Pay close attention to receptacles and to points of connection between cords and equipment. Power Outages If the IVIS Lumina S5 experiences a loss of supply power, turn off the power switches for all components and do not restart the system until reliable power has been restored. -
Page 10: Overloading
2). If the IVIS Lumina S5 is damaged and requires service, unplug the instrument from the outlet and contact PerkinElmer Technical Support. Servicing by anyone other than an authorized PerkinElmer representative voids the warranty covering the IVIS Lumina S5. -
Page 11: Chapter 3 Warnings
Contact PerkinElmer Technical Support for electrical service needs (see page 3.2 Mechanical Safety The imaging chamber of the IVIS Lumina S5 is heavy and weighs 175lbs. (80 kg). IMPORTANT: The IVIS Lumina S5 may only be moved on an IWB Workbench. See Appendix A on page 30 for instructions. -
Page 12: Chemical And Biological Safety
Dispose of all waste materials according to appropriate environmental health and safety guidelines. It is your responsibility to decontaminate the IVIS Lumina S5 before requesting service by PerkinElmer Technical Support. Ask your laboratory safety officer to advise you about the level of containment required for your application and about the proper decontamination or sterilization procedures to follow. -
Page 13: Korean Emc Registration Statement
® IVIS Lumina S5 Hardware Manual Chapter 3 | Warnings 3.5 Korean EMC Registration Statement EMC Registration is done on this equipment for business use only. The equipment may cause interference if it is used in the home. This warning statement applies to a product for business use. -
Page 14: Chapter 4 Legal Notices
Disclaimers on page 12 4.1 Introduction This manual is provided to you by PerkinElmer Health Sciences, Inc. ("PEHS") on behalf of itself and its affiliates, Caliper Life Sciences, Inc. ("Caliper") and Xenogen Corporation ("Xenogen"). PEHS, Caliper and Xenogen are referred to collectively throughout this manual as "PerkinElmer". -
Page 15: Patents
PerkinElmer warrants that such software will conform to PerkinElmer's program manuals current at the time of shipment to Customer when properly installed, provided, however, that PerkinElmer does not warrant that the operation of the software will be uninterrupted or error- free. -
Page 16: Trademarks
Chapter 4 | Legal Notices 4.4 Trademarks IVIS and Living Image are registered trademarks of PerkinElmer. The names of companies and products mentioned herein may be the trademarks of their respective owners. Microsoft and Windows are either registered trademarks or trademarks of Microsoft Corporation in the United States and/or other countries. -
Page 17: Chapter 5 Components And Specifications
Environmental Requirements on page 17 5.1 Overview The IVIS Lumina S5 is an imaging system that consists of a charged coupled device (CCD) camera that can image animal subjects (primarily mice) using bioluminescence or fluorescence modalities. IVIS Lumina S5 includes: A highly sensitive CCD camera. -
Page 18: Ccd Camera
Lumina S5 Hardware Manual Chapter 5 | Components and Specifications IMPORTANT: If you modify the IVIS Lumina S5 in any way, without prior approval from PerkinElmer, all warranties that cover this product are void. In addition, the computer included with the IVIS Lumina S5 is specifically configured to run all system-related applications. -
Page 19: Imaging Chamber Features
® IVIS Lumina S5 Hardware Manual Chapter 5 | Components and Specifications Figure 5.2 IVIS Lumina S5 – Imaging Chamber Imaging Chamber Features Custom zero-background imaging chamber 8-position emission filter wheel with 7 filters (See Table 5.1 on page 16.) -
Page 20: Optics
® The filter wheel settings are selected in the Living Image software. See the Living Image User Manual – IVIS Lumina S5 for instructions. Table 5.1 IVIS Lumina S5 Filters Excitation Filters Emission Filters... -
Page 21: Acquisition Computer
® is installed as well as the Living Image software that controls the IVIS Lumina S5. The computer controls the IVIS Lumina S5 hardware, including the CCD camera. A printer can be connected to the computer. Computer Features High speed Windows-based PC ... -
Page 22: Basic Operation
6.1 Starting the IVIS Lumina S5 Imaging System NOTE: All components of the IVIS Lumina S5 should be left on at all times. Periodically rebooting the computer is permissible and does not affect camera operation. Plug the devices into the wall sockets in the installation location. -
Page 23: Electrical Connections And Gas Plumbing
When the temperature is locked at -90°C, as indicated by the green light in the control panel, the ® instrument is ready for operation. (See the Living Image Software Manual for IVIS Lumina S5 for operating instructions.) Figure 6.2 IVIS Acquisition Control Panel in the Living Image Software... -
Page 24: Gas Plumbing
USB connection for system control electronics. Connection for accessory control. Gas Plumbing Anesthesia gas tubing is built into the IVIS Lumina S5 imaging chamber. The left side of the imaging chamber has 1/4" hose barbs marked "GAS OUT" and "GAS IN" (Figure 6.4). -
Page 25: Stage Dock And Rodent Manifold For Anesthesia
® IVIS Lumina S5 Hardware Manual Chapter 6 | Basic Operation 6.4 Stage Dock and Rodent Manifold for Anesthesia The stage dock with rodent manifold can accommodate up to five mice or two rats at a time (Figure 6.5). It supplies fresh isoflurane to keep animals anesthetized and scavenges waste gas to prevent isoflurane buildup in the imaging chamber. -
Page 26: Imaging Basics
Most plastics, almost all tape, plants, paint, rodent food (mostly plants), mouse urine, and animal bedding have been found to glow. Use caution when introducing materials into the IVIS Lumina S5. It is advisable to pre-screen all items by imaging them alone, before imaging them with samples under study. PerkinElmer... -
Page 27: Restarting The System After A Power Outage
Chapter 6 | Basic Operation 6.6 Restarting the System After a Power Outage If the IVIS Lumina S5 experiences a loss of supply power, turn off the power switch on each component. Do not restart the system until reliable line power has been restored. -
Page 28: Care And Maintenance
70% ethyl alcohol/30% deionized water solution 3 - 5% bleach (in deionized water) NOTE: PerkinElmer makes no claims as to the sterility of the IVIS Lumina S5 imaging chamber after using the solutions in Table 7.1. Please refer to the manufacturer’s literature for information as to the applicability of the compound for the organism of interest. -
Page 29: Camera Coolant
IVIS Lumina S5 Hardware Manual Chapter 7 | Care and Maintenance 7.2 Camera Coolant The IVIS Lumina S5 includes a liquid cooling system with a reservoir (located under the system cover) which maintains the camera temperature (Figure 7.1). Figure 7.1 IVIS Lumina S5 Imaging System... - Page 30 ® IVIS Lumina S5 Hardware Manual Chapter 7 | Care and Maintenance If the coolant level is lower than the top of the embossed plastic panel inside the reservoir (Figure 7.3), add Koolance 705 cooling fluid. Remove the system cover (two people are recommended because the cover is bulky). Remove the black reservoir cap.
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Page 31: Troubleshooting
® software programs the CCD camera to maintain the CCD at -90° C. If the IVIS Lumina S5 remains on, the system maintains this temperature regardless of whether the Living Image software is open or the computer is turned on. -
Page 32: Photographic Image Is Unacceptable
8.3 Luminescent Image Is Unacceptable Binning, F/Stop, and exposure time affect the appearance of a luminescent image. Please refer to the Living Image Software Manual for IVIS Lumina S5 for instructions on setting binning, exposure time, and F/Stop values. In order to function properly and reduce camera noise, the CCD camera must be cooled to the demand temperature before acquiring an image. -
Page 33: No Image Produced
Close the Living Image ® software and restart the computer. Restart the Living Image software and try to acquire an image. If you are still unable to produce an image after restarting the computer, contact PerkinElmer Technical Support for assistance (see page... -
Page 34: Appendix A Moving The Imaging System On An Iwb Workbench
Moving the Imaging System on an IWB Workbench Starting the Imaging System on page 31 This appendix explains how to move the IVIS Lumina S5 configured with the IWB Workbench. CAUTION: PerkinElmer recommends that you do not move an IVIS Lumina S5 that is not located on an IWB Workbench. -
Page 35: Starting The Imaging System
A.3 Starting the Imaging System Plug the devices into the wall sockets in the new location. Turn on the computer and monitor. Turn on the IVIS Lumina S5 imaging chamber using the power switch located on the right-hand of the instrument. ®... -
Page 36: Appendix B Options And Accessories
Appendix B Options and Accessories Table B.1 Optional Equipment and Accessories for IVIS Lumina S5 Item Part Number XBP-24 Black Paper (1 tablet of 24 sheets) 117837 XLS-4 Calibrated Light Source 118897 KH-2 Reflective Hemisphere 118937 XPM-2 Bioluminescent Phantom Mouse... -
Page 37: Index
32 specifications 17 other equipment 6 computer monitor specifications 17 contact information 2 PerkinElmer contact information 2 coolant 25 – 26 photographic image unacceptable 28 power cord 5 power outage 5 door operation 20 restarting the system 23... - Page 38 IVIS ® Lumina S5 Hardware Manual Index shut down 23 start up 18 – 19 , 31 system cover 8 Technical Support 2 temperature measured unequal to demand 27 troubleshooting camera noise 29 measured and demand unequal 27 no image produced 29 unacceptable luminescent image 28 unacceptable photographic image 28 water and moisture safety 5...