PerkinElmer OPERETTA Technical Manual
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PerkinElmer OPERETTA Technical Manual

Cellular imaging and analysis
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Summary of Contents for PerkinElmer OPERETTA

  • Page 1 ® PERETTA Technical Manual...
  • Page 2 The information contained in this document is subject to change without notice. Except as specifically set forth in its terms and conditions of sale, PerkinElmer makes no warranty of any kind with regard to this document, including, but not limited to, the implied warranties of merchantability and fitness for a particular purpose.

  • Page 3: Table Of Contents

    1.3.11 Disposal Warning Signs Protection against Harmful Interference Warranty Information Conformity Service Schedule Setup Requirements Hardware Description Introduction 2.1.1 Instrument Features Operetta Device 2.2.1 Right Side 2.2.2 Left Side 2.2.3 Front Side 2.2.4 Instrument Status 2.2.5 Sample Chamber 2.2.6 CCD Camera Temperature and CO2 Option (TCO) 2.3.1...
  • Page 4 3.2.3 Replacing a Blown Fuse 3.2.4 Transportation Maintenance by PerkinElmer Service IT Policy Network Integration 4.1.1 Overview 4.1.2 Simple Integration 4.1.3 Alternative Solutions 4.1.4 Automation Upgrade (plate::handler II) Microsoft Windows Security Updates / Service Packs Requirements for Harmony Installation on an Office PC Computer Systems 4.4.1...

  • Page 5: System Safety

    1 System Safety System Safety Operetta Technical Manual...

  • Page 6: Purpose-Oriented Application

    (biosafety levels 1 and 2, see also section 1.3.3 "Process Liquids or Substances", page 9). Due to its technical design the Operetta is particularly suitable for cellular applications. The instrument is not designed to be used in the clinical or medical-diagnostical area.

  • Page 7: Signal Words And Symbols

    Parts can suddenly start moving. Hot Surface! Concentrated laser beam! Use of controls or adjustments or performance of procedures other than those specified herein may result in hazardous radiation exposure. Electrical shock! Direct and indirect electrical contact. Operetta Technical Manual...

  • Page 8: Indication Of Hazards And Danger Zones

    Do not reach inside the Operetta through the scanning table flap. The Operetta has a moving scanning table. This table is accessible if driven out of the device for loading of new sample carriers (only for automation upgrade). An interlock switch stops driving of the table motors immediately as soon as the Operetta's lid is opened.

  • Page 9: Process Liquids Or Substances

    Always wear your personnel safety equipment. The processing of samples on the Operetta covers only probes at biosafety level 1 and 2 (BSL-1 or BSL-2, CDC http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4s3.htm). BSL-1 includes “well-characterized agents not known to consistently cause disease in healthy adult humans, and of minimal potential hazard to laboratory personnel and the environment”.

  • Page 10: Light Sources

    1 System Safety 1.3.4 Light Sources The Operetta is equipped with a class 3B autofocus laser and a high voltage Xenon lamp. Danger! Laser radiation - Eye injury Class 3B invisible laser radiation may be present inside the Operetta`s protective housing during operation. Since the housing’s...

  • Page 11: Carbon Dioxide (Only With Tco Upgrade)

    However, the flow limiter does not protect you if the carbon dioxide supply itself (or other components outside the Operetta like tubing, pressure controller, fitting etc.) is damaged or not installed correctly. To reduce the residual risk, at least one of the following measures should be carried out by the customer.

  • Page 12: Electric Shock

    1.3.7 Disconnecting from Mains Supply The on/off switch does not switch off the Operetta immediately. There will be a controlled shutdown of the electronics with a certain delay before the instrument is really switched off. Danger! Direct electrical contact - Electrical shock.

  • Page 13: Spilling Of Liquids

    Ensure the area is well ventilated. For cleaning the outer surface of the Operetta use a slightly moistened, damp cloth. For cleaning the workstation inside, the operator must specify suitable precautions, especially with regard to biological contaminated and infectious materials.

  • Page 14: Warning Signs

    Warning Signs Attention must always be paid to the black and yellow warning signs. If these signs are not attached to the system, contact the PerkinElmer customer support. Replacement labels will be supplied free of charge. Protection against Harmful Interference This equipment has been tested and found to comply with the limits for a Class B digital device, pursuant to part 15 of the FCC Rules.
  • Page 15 Maintenance work or repairs beyond the operations described in this manual may only be carried out by PerkinElmer service staff or by persons expressly authorized by PerkinElmer to do so. In case of a malfunction, contact customer support.

  • Page 16: Conformity

    1 System Safety Conformity Operetta Technical Manual...

  • Page 17: Service Schedule

    1 System Safety Service Schedule In order to guarantee a perfect and reliable function, the Operetta system should be subjected to a regular inspection by the PerkinElmer service staff. Certain maintenance tasks have to be performed regularly by the user. Please see chapter 3 “Retooling and Maintenance”...

  • Page 19: Hardware Description

    2 Hardware Description Hardware Description Operetta Technical Manual...

  • Page 20: Introduction

    The Operetta Application Guide helps you getting started with HCS. It describes the essentials of setting up an HCS assay and how to use the Operetta and image analysis software for the most common applications.

  • Page 21: Operetta Device

    Do not use an Operetta without protective shields installed if the scanning table flap is unlocked.

  • Page 22: Left Side

    2. Main switch (on/off) 3. Network (1Gbit): Connection to external control PC (must be connected to the separate network socket labeled with "OPERETTA"), not intended to connect 4. Fiber port: Port for Liquid Light Guide (Xenon lamp fiber) 5. RS232 interface for communication with Xenon light source 6.
  • Page 23 The 24 LEDs arranged in a 4×6 pattern on the lid indicate the progress of a measurement on a percentage basis. During normal operation, the sample lid of the Operetta is closed. The lid may only be opened in the following situations upon prior permission from Harmony: Exchange sample carrier: Use the Open Lid button in Harmony.

  • Page 24: Instrument Status

    2 Hardware Description 2.2.4 Instrument Status The status light in the balcony of the Operetta indicates the instrument status: Signal Description No Light Off/Dimmed The Operetta is switched off or the status light has been dimmed. Blinking Blue Pre/Post Operation Indicates ...

  • Page 25: Sample Chamber

    (see also previous chapter). 2. Scanning stage with plate holder Mechanism to hold or release the sample carrier Sample carrier bedding Above the objective lens: Swivel arm with transmission light source Operetta Technical Manual...

  • Page 26: Ccd Camera

    For brightfield measurments in combination with climatization see chapter 2.3.2 "Live Cell Chamber", page 28. 2.2.6 CCD Camera The Operetta uses one high resolution 14 bit CCD camera for cell imaging. The camera is cooled down (20 °C offset) by a Peltier element. Operetta Technical Manual...

  • Page 27: Temperature And Co2 Option (Tco)

    Option (TCO) 2.3.1 Overview If the Operetta is equipped with the TCO upgrade, the climate in the sample chamber can be controlled via the Harmony software: Temperature: 37 °C or unregulated concentration: 3 to 8.5 % (0.5 % steps) or unregulated...

  • Page 28: Live Cell Chamber

    (due to adjustment and tolerances). The Live Cell Chamber can be removed completely, e.g. for cleaning. 1. Electrical connector 2. Transmission light source Operetta Technical Manual...

  • Page 29: Co2 Supply

    2 Hardware Description 2.3.3 Supply With the TCO upgrade the Operetta's side panel is extended by a CO connection. The tubing has to be connected to your CO supply. Danger! Carbon Dioxide Gas In high concentration, carbon dioxide is harmful to health. Depending...
  • Page 30 > 15 l/h Required CO purity: ≥ 99.998 %, particle-free Notice If you connect or disconnect the Operetta from the CO supply, dust particles may be introduced into the tubing. This can lead to malfunctions and blockages. Blow through the tubing using CO before reconnecting it to the Operetta.

  • Page 31: Control Pc (Harmony Pc)

    2 Hardware Description Control PC (Harmony PC) The Operetta system is equipped with a control PC, TFT monitor, mouse and keyboard. The user can control the Operetta using the Harmony software installed on the control PC. The Harmony software allows for fully automated image acquisition, online image evaluation and data management.

  • Page 32: Additional Upgrades

    Option (TCO) See section 2.3 "Temperature and CO2 Option (TCO)", page 27. Field upgrades The confocal upgrade, more excitation filters, objectives, automation components (plate::handler II, plate hotel, incubator) and server extensions can be provided as field upgrades. Operetta Technical Manual...

  • Page 33: Retooling And Maintenance

    3 Retooling and Maintenance Retooling and Maintenance Operetta Technical Manual...

  • Page 34: Retooling

    3 Retooling and Maintenance This chapter describes retooling tasks for changing the Operetta`s optical setup and maintenance procedures, which need to be carried out periodically to keep the instrument in good operating condition. The individual steps for each task are described and a schedule for the maintenance tasks is given.
  • Page 35 You can avoid this if you let the objective warm up before you screw it in, e.g. by putting it on the glass plate inside the Operetta for 30-60 minutes.

  • Page 36: Correction Collar

    3.1.2 Correction Collar Some objective lenses for the Operetta (see table on the next page) have an integrated correction collar. It can be rotated and allows the correction of spherical aberrations mainly caused by differences in plate bottom thickness. Underneath the colored ring of the objective you can find a small white line which should be set directly on the value of the plate bottom thickness.
  • Page 37 2. Turn the correction collar and adjust it to the given value [mm]. 3. If the mark of the correction collar is not visible from your position, use the provided mirror to adjust for the correct value. Operetta Technical Manual...

  • Page 38: Emission Filter

    3.1.3 Emission Filter The Operetta is equipped with an eight position emission filter wheel. The four emission filters 410 - 480 nm, 500 – 550 nm, 560 – 630 nm and 650 – 760 nm are always included in the instrument. All of the other selections can be chosen as an option at any time for customer installation.
  • Page 39 3. Insert the new filter to the intended position. 4. Close the door. The filter wheel starts homing and the filter information will be read by the internal barcode reader. 5. A summary table shows all installed filters.Click OK to close the wizard. Operetta Technical Manual...

  • Page 40: User Maintenance

    Repeated ignition attempts without lamp going into operation. Low light output evident with dark picture at high light level adjustment. The delivery of the Operetta is carried out including a replacement lamp. Alternatively the whole lamp module can be easily exchanged, which has to be ordered separately.

  • Page 41: Cleaning

    The objective lens(es) should only be cleaned if they are obviously soiled or dusty. Shut down the Operetta to move the scanning table to the service position. Wet the objective lens with 70% ethanol. Take a dust-free optical tissue, wipe the lens a few times very carefully without force and let it dry.

  • Page 42: Replacing A Blown Fuse

    3 Retooling and Maintenance 3.2.3 Replacing a Blown Fuse If one of the two fuses of the Operetta has blown, it can be exchanged by the user. Danger! Electric Shock! Switch off the Operetta and disconnect the power cable before changing the fuse(s).

  • Page 43: Transportation

    3 Retooling and Maintenance 3.2.4 Transportation It is only permitted to move the Operetta on the bench by four persons. Warning! The four handles of the Operetta are not designed to carry the device for relocation! The instrument is very heavy (120 kg). If it falls down or tips over it can cause severe injuries.

  • Page 44: It Policy

    4 IT Policy IT Policy Operetta Technical Manual...

  • Page 45: Network Integration

    Operetta System into any network environment. Notice The purpose of the Operetta IT Policy is to ensure the effective protection and proper usage of the computer systems belonging to the "Operetta System". The IT Policy will assist in maintaining systems at operational level.

  • Page 46: Simple Integration

    4 IT Policy Notice The Harmony PC is a dedicated computer to control the ® Operetta. This PC runs with Windows 7 (64 bit), includes the database and cannot be substituted by any customer-supplied computer. The optional Columbus software (especially the Acapella Server) may not be installed on the Harmony PC or on a PC with Harmony office installation.

  • Page 47: Alternative Solutions

    Alternative Solutions ® In the case where Windows 7 is not allowed in the customer's company network, use one of the following solutions to isolate the "Operetta network" from the company network. 4.1.3.1 Office PC as interface (small solution) 4.1.3.2...
  • Page 48 4 IT Policy 4.1.3.3 Router Operetta Technical Manual...

  • Page 49: Automation Upgrade (Plate::handler Ii)

    PC (both in red, see figure above) are configured with static IP addresses by your local IT department. The network configuration (IP, Gateway, Subnet, Wins, DNS) has to be adapted according to the configuration of the company network. Operetta Technical Manual...

  • Page 50: Microsoft Windows Security Updates / Service Packs

    PerkinElmer cannot guarantee that future Windows updates provided by Microsoft will not compromise the stability of the "Operetta System". We assume that the impact is low but to ensure to always have a stable "Operetta System" it is recommended to disable Windows updates.

  • Page 51: Requirements For Harmony Installation On An Office Pc

    PerkinElmer installation of a computer supplied by the customer is available for an additional fee. PerkinElmer is not responsible for problems caused by unspecified system components, software, and/or accessories.

  • Page 52: Computer Systems

    The Harmony PC can receive the network address from a DHCP server. The general configuration is to obtain the IP address automatically from a DHCP. 1. The usage of any additional network adapter of any computer of the "Operetta System" is not allowed.

  • Page 53: Data

    4.4.5 Back Up 1. PerkinElmer is not responsible for the implementation of an effective backup strategy. 2. PerkinElmer is not responsible for the backup of any files from the "Operetta System". 4.4.6 Security Settings & Anti-Virus Protection The Harmony software is tested with Microsoft Forefront Client Security.

  • Page 54: Remote Support

    Remote Support PerkinElmer uses the service of WebEx (http://evoportal.webex.com) to solve remote support issues in real time for "Operetta Systems". If WebEx support is not allowed please contact PerkinElmer Service and ask for other options. (Please note: Other options may increase costs for the service contract) Minimum system requirement for WebEx remote support: ®...

  • Page 55: Technical Data

    5 Technical Data Technical Data Operetta Technical Manual...

  • Page 56: Optical Configuration

    The Operetta has a high speed high precision auto focus unit based on an infrared laser for detection of the sample carrier surface and measurements in a defined imaging plane above the sample carrier surface.
  • Page 57 CCD camera. Special optics enable viewing of an enlarged field of view / number of cells in an image view without compromising optical resolution. The Operetta unit contains all necessary hardware and software for four color measurements (UV, green, orange and far red) using a 20x long WD objective – see below for the accessories included.

  • Page 58: Basic Components

    Xenon light source External Xenon lamp, fiber-coupled Spectral width: 14 nm General wavelength range: 350-680 nm Input Voltage: 100 – 240 V AC, 50/60 Hz, 6.0A Lamp module: 300 W Lifetime: 500 hours Easy exchange Brightfield LED Operetta Technical Manual...

  • Page 59: Specifications

    60x high NA 225 × 170 0.33 100x long WD 0.85 1.2-1.9 135 × 102 0.35 5.3.2 Sample Carrier Recommended sample carriers for the Operetta: Plate type Bottom Plate bottom Suitable for material allows use of the following supports high NA...

  • Page 60: Power Supply

    60x high NA* 60x long WD 100x long WD* * Restrictions - some edge wells cannot be measured 5.3.3 Power Supply The Operetta is connected to mains by a separate power cable which is specified as follows: Specifications Europe Cordage Rating 16A/250V~AC...
  • Page 62 PerkinElmer Schnackenburgallee 114 22525 Hamburg Germany Internet: http://www.perkinelmer.com email: info@perkinelmer.com...

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