Setting Of Recorder And Spectrum Output Factors - PerkinElmer 200A Series User Manual

<Scan monitor screen (spectral acquisition under way)>
g. When the spectral data memorization is completed, the system returns to the 1st monitor

Setting of Recorder and Spectrum Output Factors

The following items are settable as factors used in recorder and/or spectrum output.
• Full scale range of recorder
•
Output speed of recorder
• Output spectrum data
With an emission spectrum, scattered excitation light, Raman scattering due to solvent, emission due to
NOTE:
solvent or impurities in it, and second (or third) order emission may be superimposed on the emission of
the analyte element. Similarly, an excitation spectrum may be affected by scattered light at the emission
wavelength, by Raman scattering due to solvent, by emission due to solvent or impurities contained in it,
and by second (or third) order scattered light of the excitation beam. These factors must be taken into
consideration when selecting the optimum excitation and emission wavelengths for spectrum acquisition.
Refer to section 3.4 for details.
The excitation and emission spectra output by the Series 200a are not corrected for the wavelength-
NOTE:
dependent sensitivity of the photomultiplier. The obtained spectra therefore differ from the true
excitation and emission spectra of the analyte element.
This difference is especially noticeable at wavelengths below 300 nm.
Initial (default) value: blank
Upon entering a memorize spectrum No., the previous spectral data will be
deleted and the new data will be memorized.
When the Xe lamp goes off, press the ENT key and the 1st monitor screen will
return.
f. The scan monitor screen appears, and spectral acquisition within the set range is
carried out.
SCANNING SPECTRUM NO.: 1 MODE: FL (Em)
Ex: 250 nm Em: *** nm
Pressing ESC key during spectral acquisition will cancel the process. Spectral data
will not be memorized.
The memorize spectrum No. set in the preceding step will be indicated at SCANNING
SPECTRUM NO.
Em is effective within the scan range set above.
screen automatically.
1. Full Scale Range of Recorder
a. Press the RECORD key.
b. The recorder setting screen appears.
<Recorder setting screen>
Operation
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