Description Of Fluorometry; Principle Of Fluorometry - PerkinElmer 200A Series User Manual

Series 200a Fi User's Guide
44.

Description of Fluorometry

Principle of Fluorometry

Excitation
Excited state V =
Excited triplet
state
Excitation light
Ground state V =
Fig. 3-5 Typical Organic Molecular Energy Level Diagram
Stable condition
(Ground state)
Radiationless
transition
3
2
1
0
3
2
1
0
Absorption
Fluorescence or
Phosphorescence
Radiationless
transition
Light
Fluorescence
Unstable condition
(Excited state)
Light
Phosphorescence