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Series 200a Fl User's Guide
120.
Performance Check
Check the performance when the instrument has been installed.
NOTE:
First adjust the xenon lamp position and then check the performance. Positional adjustment of the
lamp is necessary for an accurate performance check.
When this detector unit is used in combination with the EZChrom Elite Client/Server, the lamp
NOTE:
energy, wavelength accuracy and sensitivity can be automatically calculated. For details, refer to
the manual that comes with this option..
Energy Check of Xe Lamp
Check the energy value of the Xe lamp at a wavelength of 254 nm (see chapter 5, Operation).
When the energy value of the Xe lamp has decreased by 50% or more in comparison with the
NOTE:
value at the start of use, the useful life of the lamp is considered to have expired. Use the
decrease in energy value plus baseline fluctuation (data variation or noise) in judging the lifetime of
the lamp.
Check of Wavelength Accuracy by Using Hg Lamp
Check the wavelength accuracy by using the bright line at 254 nm of the Hg lamp (see chapter 5,
Operation
). GOOD will appear on the display when the wavelength error is within (3 nm, or "FAIL"
will appear when the error exceeds 3 nm.
Wavelength calibration is necessary when "FAIL" appears. See the procedure for the calibration,
NOTE:
later in this chapter.
Check of Sensitivity
The S/N ratio of the Raman spectrum of water is used to measure the sensitivity of the
fluorescence detector. The following items must be prepared for the measurement.
The following items are required.
•
Pump
•
Recorder
•
Distilled water
1. Injection of Distilled Water Connect tubing as shown in Fig. 6-1, supply distilled water at a flow
rate of 1 mL/min for 3 minutes with the Series 200a pump in Manual mode, then set the flow
rate to 0 and stop the supply of water. Remove the flow cell unit and check for any bubbles in
the flow cell.
If the flow cell has been left empty for awhile (including when the instrument is installed), first
NOTE:
inject methanol or acetonitrile into the flow cell in order to wet the interior in advance, and then
inject distilled water. Injection of water without first injecting an organic solvent may cause a
reduction of sensitivity.
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Troubleshooting
